[Bacteroides] pectinophilus ATCC 43243
Average proteome isoelectric point is 6.09
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3243 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B7APT6|B7APT6_9FIRM ABC transmembrane type-1 domain-containing protein OS=[Bacteroides] pectinophilus ATCC 43243 OX=483218 GN=BACPEC_00692 PE=3 SV=1
MM1 pKa = 6.83 NRR3 pKa = 11.84 KK4 pKa = 8.03 TIFCFLTALIMIFVLAGCGGKK25 pKa = 9.96 NSGGNIDD32 pKa = 4.19 NVQIPDD38 pKa = 3.59 WKK40 pKa = 10.24 PSEE43 pKa = 4.49 IYY45 pKa = 10.37 SDD47 pKa = 3.72 SDD49 pKa = 3.24 IEE51 pKa = 4.76 AAFQTVKK58 pKa = 10.83 DD59 pKa = 3.89 YY60 pKa = 11.24 FGNEE64 pKa = 3.64 FDD66 pKa = 4.53 GCTLTKK72 pKa = 9.73 LTYY75 pKa = 10.45 PGDD78 pKa = 3.66 TYY80 pKa = 11.74 ADD82 pKa = 3.72 EE83 pKa = 4.89 FYY85 pKa = 10.92 EE86 pKa = 4.23 WAEE89 pKa = 3.95 QYY91 pKa = 10.84 DD92 pKa = 3.94 ADD94 pKa = 4.06 EE95 pKa = 5.71 AIVILSSFDD104 pKa = 3.37 VDD106 pKa = 3.7 SSGGDD111 pKa = 3.21 GSLNPDD117 pKa = 2.97 STYY120 pKa = 11.61 DD121 pKa = 3.2 DD122 pKa = 3.88 WKK124 pKa = 10.55 WILIRR129 pKa = 11.84 NDD131 pKa = 3.03 SGNWEE136 pKa = 4.45 HH137 pKa = 6.93 VDD139 pKa = 2.97 HH140 pKa = 7.19 GYY142 pKa = 11.09
Molecular weight: 15.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.731
IPC2_protein 3.821
IPC_protein 3.821
Toseland 3.592
ProMoST 3.999
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.77
Rodwell 3.643
Grimsley 3.503
Solomon 3.808
Lehninger 3.77
Nozaki 3.935
DTASelect 4.202
Thurlkill 3.656
EMBOSS 3.783
Sillero 3.948
Patrickios 0.744
IPC_peptide 3.808
IPC2_peptide 3.923
IPC2.peptide.svr19 3.824
Protein with the highest isoelectric point:
>tr|B7AU99|B7AU99_9FIRM Guanylate kinase-like domain-containing protein OS=[Bacteroides] pectinophilus ATCC 43243 OX=483218 GN=BACPEC_02297 PE=4 SV=1
MM1 pKa = 7.67 KK2 pKa = 8.72 MTFQPKK8 pKa = 9.06 KK9 pKa = 8.03 RR10 pKa = 11.84 SRR12 pKa = 11.84 SKK14 pKa = 9.44 VHH16 pKa = 6.17 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MSTPGGRR28 pKa = 11.84 KK29 pKa = 8.7 VLAARR34 pKa = 11.84 RR35 pKa = 11.84 LKK37 pKa = 10.61 GRR39 pKa = 11.84 KK40 pKa = 8.87 KK41 pKa = 10.59 LSAA44 pKa = 3.95
Molecular weight: 5.04 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.494
IPC2_protein 11.125
IPC_protein 12.691
Toseland 12.866
ProMoST 13.349
Dawson 12.866
Bjellqvist 12.852
Wikipedia 13.334
Rodwell 12.676
Grimsley 12.896
Solomon 13.349
Lehninger 13.247
Nozaki 12.866
DTASelect 12.852
Thurlkill 12.866
EMBOSS 13.349
Sillero 12.866
Patrickios 12.398
IPC_peptide 13.349
IPC2_peptide 12.34
IPC2.peptide.svr19 9.084
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3243
0
3243
921347
39
3037
284.1
31.81
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.874 ± 0.054
1.542 ± 0.02
6.371 ± 0.037
6.771 ± 0.043
3.862 ± 0.028
6.889 ± 0.044
1.675 ± 0.016
7.853 ± 0.046
6.53 ± 0.037
8.055 ± 0.04
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.349 ± 0.025
4.954 ± 0.031
2.931 ± 0.022
2.963 ± 0.029
4.609 ± 0.041
6.177 ± 0.039
5.429 ± 0.051
6.952 ± 0.037
0.823 ± 0.014
4.39 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here