Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) (Methylobacterium populi)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylorubrum; Methylorubrum populi

Average proteome isoelectric point is 6.85

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5325 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B1ZGJ2|B1ZGJ2_METPB Transcriptional regulator XRE family OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) OX=441620 GN=Mpop_3071 PE=4 SV=1
MM1 pKa = 7.8SIPSYY6 pKa = 11.25ARR8 pKa = 11.84TNFQTLLRR16 pKa = 11.84AAGDD20 pKa = 3.71GNLALMEE27 pKa = 4.78CLDD30 pKa = 4.41AVTGSPRR37 pKa = 11.84YY38 pKa = 9.11VICAVGQDD46 pKa = 3.47DD47 pKa = 4.22GDD49 pKa = 4.12YY50 pKa = 10.58VFTPFGHH57 pKa = 7.13LADD60 pKa = 4.52GNPYY64 pKa = 10.31DD65 pKa = 5.22AYY67 pKa = 11.03LPPDD71 pKa = 4.39PDD73 pKa = 3.83DD74 pKa = 4.4PEE76 pKa = 5.52GFVRR80 pKa = 11.84PDD82 pKa = 3.0AGEE85 pKa = 3.94APP87 pKa = 3.83

Molecular weight: 9.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B1ZB75|B1ZB75_METPB pP_pnuc_2 domain-containing protein OS=Methylorubrum populi (strain ATCC BAA-705 / NCIMB 13946 / BJ001) OX=441620 GN=Mpop_2510 PE=4 SV=1
MM1 pKa = 7.34IRR3 pKa = 11.84KK4 pKa = 8.9LLTALVLPRR13 pKa = 11.84VIAFIGRR20 pKa = 11.84RR21 pKa = 11.84LSGRR25 pKa = 11.84AAAPPRR31 pKa = 11.84HH32 pKa = 5.58GRR34 pKa = 3.42

Molecular weight: 3.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5325

0

5325

1649263

30

14829

309.7

33.26

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.141 ± 0.058

0.798 ± 0.013

5.516 ± 0.033

5.752 ± 0.041

3.386 ± 0.026

9.134 ± 0.061

1.939 ± 0.021

4.331 ± 0.03

2.542 ± 0.024

10.444 ± 0.049

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.001 ± 0.023

2.103 ± 0.027

5.828 ± 0.048

2.755 ± 0.019

8.256 ± 0.056

5.014 ± 0.034

5.342 ± 0.053

7.492 ± 0.033

1.24 ± 0.014

1.985 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski