Clostridium sp. CAG:964

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae; Clostridium; environmental samples

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1860 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6UES8|R6UES8_9CLOT Deoxyguanosinetriphosphate triphosphohydrolase-like protein OS=Clostridium sp. CAG:964 OX=1262848 GN=BN818_00425 PE=3 SV=1
MM1 pKa = 7.75RR2 pKa = 11.84KK3 pKa = 9.19LAVILFSALILTVGGAILTGCGCSNSEE30 pKa = 4.31SSSTSSGSASTEE42 pKa = 3.7PKK44 pKa = 10.15KK45 pKa = 10.85DD46 pKa = 3.52VVGNWGGRR54 pKa = 11.84SDD56 pKa = 3.94NANASFNDD64 pKa = 3.26NGTCEE69 pKa = 3.97IGGVSGTYY77 pKa = 10.0TIDD80 pKa = 3.42KK81 pKa = 10.37DD82 pKa = 3.7NKK84 pKa = 9.76LTVTPNSSDD93 pKa = 3.57GTSTTPMTFDD103 pKa = 4.09YY104 pKa = 10.06YY105 pKa = 10.95TDD107 pKa = 3.59SNYY110 pKa = 10.84SSVPQNQWAIANNTLYY126 pKa = 10.59INGYY130 pKa = 9.88QYY132 pKa = 11.22TKK134 pKa = 10.75DD135 pKa = 3.68SYY137 pKa = 11.55SGQSDD142 pKa = 4.02SNSDD146 pKa = 3.29NSSSGNSSNNSSGSSSNSSSSGSSASGNNSSSSSSSASGNNSSSTLNEE194 pKa = 3.67GDD196 pKa = 3.72EE197 pKa = 4.56SDD199 pKa = 3.57MVTIVDD205 pKa = 4.71NIDD208 pKa = 3.8DD209 pKa = 3.96FASS212 pKa = 3.28

Molecular weight: 21.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6VEH6|R6VEH6_9CLOT Putative stage III sporulation protein AF OS=Clostridium sp. CAG:964 OX=1262848 GN=BN818_01191 PE=4 SV=1
MM1 pKa = 7.55NNRR4 pKa = 11.84FNNQNSQNNQSKK16 pKa = 10.5SSQNNSQNNNQHH28 pKa = 6.16NSQNNNRR35 pKa = 11.84SNSQNNNQNNRR46 pKa = 11.84SSQNKK51 pKa = 8.31NRR53 pKa = 11.84NSNFF57 pKa = 3.14

Molecular weight: 6.67 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1860

0

1860

586356

29

3136

315.2

35.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.592 ± 0.057

1.686 ± 0.024

5.743 ± 0.048

6.531 ± 0.062

4.053 ± 0.039

6.752 ± 0.054

1.452 ± 0.024

7.592 ± 0.056

7.547 ± 0.046

8.393 ± 0.055

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.679 ± 0.028

5.406 ± 0.052

3.213 ± 0.033

3.087 ± 0.034

3.802 ± 0.047

6.63 ± 0.06

5.807 ± 0.057

7.136 ± 0.047

0.71 ± 0.016

4.188 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski