Pichia membranifaciens

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Saccharomycotina; Saccharomycetes; Saccharomycetales; Pichiaceae; Pichia

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4160 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Q2YCS2|A0A1Q2YCS2_9ASCO J domain-containing protein OS=Pichia membranifaciens OX=4926 GN=PMKS-000762 PE=4 SV=1
MM1 pKa = 7.14MMSSYY6 pKa = 10.34FQSTYY11 pKa = 10.07NSGRR15 pKa = 11.84IAQTYY20 pKa = 10.23LLASKK25 pKa = 10.46VKK27 pKa = 10.81AKK29 pKa = 8.93LTQEE33 pKa = 3.95AVRR36 pKa = 11.84NDD38 pKa = 3.29VDD40 pKa = 3.6LHH42 pKa = 7.25RR43 pKa = 11.84LVCQANLLDD52 pKa = 4.82NLIEE56 pKa = 4.25NLNHH60 pKa = 7.02HH61 pKa = 6.28EE62 pKa = 4.46SSYY65 pKa = 11.87NNNNSNTTGNTNICYY80 pKa = 10.24EE81 pKa = 4.55SINSNSYY88 pKa = 9.74PLLNNVSVQVNNLDD102 pKa = 3.61YY103 pKa = 11.34SVDD106 pKa = 3.7EE107 pKa = 5.54DD108 pKa = 5.5ADD110 pKa = 3.84SDD112 pKa = 5.02DD113 pKa = 4.63NDD115 pKa = 4.01EE116 pKa = 4.81EE117 pKa = 5.51DD118 pKa = 5.95DD119 pKa = 4.3EE120 pKa = 5.66VDD122 pKa = 3.56EE123 pKa = 4.6NDD125 pKa = 3.6IKK127 pKa = 10.8IEE129 pKa = 3.94RR130 pKa = 11.84APCSDD135 pKa = 3.3VYY137 pKa = 10.22YY138 pKa = 10.53TSDD141 pKa = 4.79DD142 pKa = 3.7SDD144 pKa = 3.98FDD146 pKa = 4.97SDD148 pKa = 4.91DD149 pKa = 5.19DD150 pKa = 4.68SDD152 pKa = 4.55SSFNSDD158 pKa = 2.94SFGGKK163 pKa = 9.74SSDD166 pKa = 3.94PNEE169 pKa = 4.3LCCIALQRR177 pKa = 11.84LNLHH181 pKa = 6.38GGVDD185 pKa = 4.02EE186 pKa = 6.84DD187 pKa = 5.48IDD189 pKa = 6.02DD190 pKa = 5.09DD191 pKa = 5.69DD192 pKa = 5.81SGNQEE197 pKa = 3.79NCSSVTATITLTDD210 pKa = 4.04SEE212 pKa = 4.96SDD214 pKa = 3.17SDD216 pKa = 4.51SNSEE220 pKa = 4.19TEE222 pKa = 4.71SDD224 pKa = 3.38TEE226 pKa = 4.23YY227 pKa = 11.08EE228 pKa = 3.95NDD230 pKa = 3.51SRR232 pKa = 11.84DD233 pKa = 3.6GLFSNNYY240 pKa = 7.46CALVRR245 pKa = 11.84MHH247 pKa = 6.32SQHH250 pKa = 6.45TSLNLNDD257 pKa = 3.78YY258 pKa = 9.46VASLEE263 pKa = 4.07ADD265 pKa = 3.48TSVEE269 pKa = 4.05EE270 pKa = 4.22TDD272 pKa = 3.31SCKK275 pKa = 10.7NIQHH279 pKa = 6.21TSSLDD284 pKa = 3.25EE285 pKa = 4.0MTRR288 pKa = 11.84EE289 pKa = 3.8EE290 pKa = 4.84DD291 pKa = 4.46LPSLSNCSSFSSMEE305 pKa = 4.55EE306 pKa = 3.2ISQPNQNIVRR316 pKa = 11.84LEE318 pKa = 4.09CLDD321 pKa = 3.88SIKK324 pKa = 10.23PQRR327 pKa = 11.84DD328 pKa = 2.99TDD330 pKa = 4.03SPSLYY335 pKa = 9.2LTQSQDD341 pKa = 2.98VFLLL345 pKa = 3.98

Molecular weight: 38.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Q2YIB5|A0A1Q2YIB5_9ASCO DNA-directed RNA polymerases I II and III subunit RPABC1 OS=Pichia membranifaciens OX=4926 GN=PMKS-002769 PE=3 SV=1
MM1 pKa = 7.46ISARR5 pKa = 11.84SIKK8 pKa = 10.29AAAKK12 pKa = 9.64QALAVNARR20 pKa = 11.84IVPRR24 pKa = 11.84LVRR27 pKa = 11.84AYY29 pKa = 9.85HH30 pKa = 5.58QRR32 pKa = 11.84AQGGEE37 pKa = 3.91EE38 pKa = 4.12RR39 pKa = 11.84IAAAACSTRR48 pKa = 11.84ARR50 pKa = 11.84PLAARR55 pKa = 11.84TYY57 pKa = 10.85ASAKK61 pKa = 9.17AQPTEE66 pKa = 3.82VSSILEE72 pKa = 3.96SKK74 pKa = 10.2IRR76 pKa = 11.84GVSEE80 pKa = 3.66EE81 pKa = 4.35ANLDD85 pKa = 3.29EE86 pKa = 4.69TGRR89 pKa = 11.84VLAVGYY95 pKa = 10.47VLLSQQ100 pKa = 4.29

Molecular weight: 10.63 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4160

0

4160

2159257

66

4922

519.1

58.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.003 ± 0.037

1.115 ± 0.012

6.429 ± 0.033

7.073 ± 0.039

4.213 ± 0.027

5.383 ± 0.033

1.993 ± 0.014

6.203 ± 0.033

7.442 ± 0.035

9.175 ± 0.045

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.08 ± 0.013

6.068 ± 0.041

4.218 ± 0.031

3.803 ± 0.033

4.174 ± 0.025

9.076 ± 0.053

5.511 ± 0.029

5.817 ± 0.025

0.922 ± 0.011

3.3 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski