Amino acid dipepetide frequency for Geothermobacter sp. EPR-M

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	11.64AlaAla: 11.64 ± 0.141	1.3AlaCys: 1.3 ± 0.044	5.87AlaAsp: 5.87 ± 0.075	7.302AlaGlu: 7.302 ± 0.092	3.331AlaPhe: 3.331 ± 0.054	9.284AlaGly: 9.284 ± 0.117	1.533AlaHis: 1.533 ± 0.042	4.994AlaIle: 4.994 ± 0.077	2.882AlaLys: 2.882 ± 0.06	10.473AlaLeu: 10.473 ± 0.112	2.36AlaMet: 2.36 ± 0.041	2.29AlaAsn: 2.29 ± 0.053	3.726AlaPro: 3.726 ± 0.063	2.497AlaGln: 2.497 ± 0.046	6.965AlaArg: 6.965 ± 0.088	4.09AlaSer: 4.09 ± 0.08	4.485AlaThr: 4.485 ± 0.079	7.015AlaVal: 7.015 ± 0.086	1.053AlaTrp: 1.053 ± 0.032	2.072AlaTyr: 2.072 ± 0.047	0.0AlaXaa: 0.0 ± 0.0
Cys	1.024CysAla: 1.024 ± 0.032	0.27CysCys: 0.27 ± 0.017	0.754CysAsp: 0.754 ± 0.03	0.646CysGlu: 0.646 ± 0.027	0.51CysPhe: 0.51 ± 0.024	1.444CysGly: 1.444 ± 0.043	0.711CysHis: 0.711 ± 0.092	0.539CysIle: 0.539 ± 0.021	0.326CysLys: 0.326 ± 0.019	1.311CysLeu: 1.311 ± 0.04	0.233CysMet: 0.233 ± 0.016	0.355CysAsn: 0.355 ± 0.019	0.798CysPro: 0.798 ± 0.031	0.38CysGln: 0.38 ± 0.019	1.266CysArg: 1.266 ± 0.04	0.84CysSer: 0.84 ± 0.031	0.528CysThr: 0.528 ± 0.026	0.662CysVal: 0.662 ± 0.026	0.158CysTrp: 0.158 ± 0.012	0.394CysTyr: 0.394 ± 0.021	0.0CysXaa: 0.0 ± 0.0
Asp	4.782AspAla: 4.782 ± 0.073	0.794AspCys: 0.794 ± 0.026	3.025AspAsp: 3.025 ± 0.062	3.636AspGlu: 3.636 ± 0.062	2.549AspPhe: 2.549 ± 0.048	4.613AspGly: 4.613 ± 0.085	1.265AspHis: 1.265 ± 0.036	3.222AspIle: 3.222 ± 0.058	2.049AspLys: 2.049 ± 0.049	6.951AspLeu: 6.951 ± 0.091	1.108AspMet: 1.108 ± 0.032	1.769AspAsn: 1.769 ± 0.044	3.066AspPro: 3.066 ± 0.059	2.112AspGln: 2.112 ± 0.042	4.265AspArg: 4.265 ± 0.072	2.743AspSer: 2.743 ± 0.051	2.108AspThr: 2.108 ± 0.051	3.307AspVal: 3.307 ± 0.065	0.764AspTrp: 0.764 ± 0.027	1.947AspTyr: 1.947 ± 0.039	0.0AspXaa: 0.0 ± 0.0
Glu	5.838GluAla: 5.838 ± 0.093	0.501GluCys: 0.501 ± 0.02	3.161GluAsp: 3.161 ± 0.059	4.861GluGlu: 4.861 ± 0.09	2.137GluPhe: 2.137 ± 0.044	4.22GluGly: 4.22 ± 0.061	1.203GluHis: 1.203 ± 0.034	4.329GluIle: 4.329 ± 0.074	3.789GluLys: 3.789 ± 0.071	7.474GluLeu: 7.474 ± 0.1	1.74GluMet: 1.74 ± 0.04	2.046GluAsn: 2.046 ± 0.044	2.33GluPro: 2.33 ± 0.049	3.536GluGln: 3.536 ± 0.07	4.687GluArg: 4.687 ± 0.085	2.847GluSer: 2.847 ± 0.052	3.546GluThr: 3.546 ± 0.058	4.694GluVal: 4.694 ± 0.066	0.521GluTrp: 0.521 ± 0.024	1.324GluTyr: 1.324 ± 0.039	0.0GluXaa: 0.0 ± 0.0
Phe	3.639PheAla: 3.639 ± 0.069	0.697PheCys: 0.697 ± 0.023	2.508PheAsp: 2.508 ± 0.049	2.19PheGlu: 2.19 ± 0.051	1.904PhePhe: 1.904 ± 0.047	3.406PheGly: 3.406 ± 0.063	0.788PheHis: 0.788 ± 0.028	2.053PheIle: 2.053 ± 0.048	1.367PheLys: 1.367 ± 0.032	4.052PheLeu: 4.052 ± 0.068	0.822PheMet: 0.822 ± 0.029	1.366PheAsn: 1.366 ± 0.032	1.782PhePro: 1.782 ± 0.045	1.2PheGln: 1.2 ± 0.032	2.44PheArg: 2.44 ± 0.051	2.95PheSer: 2.95 ± 0.049	1.942PheThr: 1.942 ± 0.043	2.403PheVal: 2.403 ± 0.047	0.464PheTrp: 0.464 ± 0.023	1.159PheTyr: 1.159 ± 0.035	0.0PheXaa: 0.0 ± 0.0
Gly	6.451GlyAla: 6.451 ± 0.094	1.411GlyCys: 1.411 ± 0.044	4.442GlyAsp: 4.442 ± 0.081	5.277GlyGlu: 5.277 ± 0.071	3.328GlyPhe: 3.328 ± 0.058	6.756GlyGly: 6.756 ± 0.106	1.797GlyHis: 1.797 ± 0.044	4.999GlyIle: 4.999 ± 0.072	3.783GlyLys: 3.783 ± 0.065	9.017GlyLeu: 9.017 ± 0.12	2.309GlyMet: 2.309 ± 0.057	2.53GlyAsn: 2.53 ± 0.063	2.739GlyPro: 2.739 ± 0.047	2.929GlyGln: 2.929 ± 0.05	6.241GlyArg: 6.241 ± 0.089	4.667GlySer: 4.667 ± 0.087	4.25GlyThr: 4.25 ± 0.081	5.657GlyVal: 5.657 ± 0.071	1.051GlyTrp: 1.051 ± 0.037	2.448GlyTyr: 2.448 ± 0.05	0.0GlyXaa: 0.0 ± 0.0
His	1.656HisAla: 1.656 ± 0.047	0.363HisCys: 0.363 ± 0.021	1.2HisAsp: 1.2 ± 0.039	0.963HisGlu: 0.963 ± 0.029	0.994HisPhe: 0.994 ± 0.031	1.854HisGly: 1.854 ± 0.051	0.579HisHis: 0.579 ± 0.027	0.972HisIle: 0.972 ± 0.029	0.599HisLys: 0.599 ± 0.025	2.4HisLeu: 2.4 ± 0.053	0.376HisMet: 0.376 ± 0.017	0.644HisAsn: 0.644 ± 0.028	1.556HisPro: 1.556 ± 0.041	0.858HisGln: 0.858 ± 0.028	1.513HisArg: 1.513 ± 0.035	1.108HisSer: 1.108 ± 0.036	0.811HisThr: 0.811 ± 0.027	1.073HisVal: 1.073 ± 0.036	0.285HisTrp: 0.285 ± 0.016	0.662HisTyr: 0.662 ± 0.025	0.0HisXaa: 0.0 ± 0.0
Ile	5.377IleAla: 5.377 ± 0.077	0.767IleCys: 0.767 ± 0.027	3.764IleAsp: 3.764 ± 0.064	3.737IleGlu: 3.737 ± 0.066	2.125IlePhe: 2.125 ± 0.048	4.485IleGly: 4.485 ± 0.073	1.186IleHis: 1.186 ± 0.033	2.847IleIle: 2.847 ± 0.06	2.028IleLys: 2.028 ± 0.047	5.699IleLeu: 5.699 ± 0.092	1.032IleMet: 1.032 ± 0.029	1.924IleAsn: 1.924 ± 0.043	2.834IlePro: 2.834 ± 0.053	1.539IleGln: 1.539 ± 0.04	3.821IleArg: 3.821 ± 0.063	3.407IleSer: 3.407 ± 0.055	2.485IleThr: 2.485 ± 0.056	3.401IleVal: 3.401 ± 0.064	0.521IleTrp: 0.521 ± 0.02	1.479IleTyr: 1.479 ± 0.04	0.0IleXaa: 0.0 ± 0.0
Lys	3.596LysAla: 3.596 ± 0.065	0.32LysCys: 0.32 ± 0.019	1.94LysAsp: 1.94 ± 0.043	2.473LysGlu: 2.473 ± 0.049	1.152LysPhe: 1.152 ± 0.035	2.956LysGly: 2.956 ± 0.056	0.697LysHis: 0.697 ± 0.027	2.56LysIle: 2.56 ± 0.058	2.547LysLys: 2.547 ± 0.063	4.193LysLeu: 4.193 ± 0.078	1.026LysMet: 1.026 ± 0.035	1.404LysAsn: 1.404 ± 0.038	1.873LysPro: 1.873 ± 0.047	1.698LysGln: 1.698 ± 0.042	2.547LysArg: 2.547 ± 0.054	1.975LysSer: 1.975 ± 0.045	2.25LysThr: 2.25 ± 0.049	2.995LysVal: 2.995 ± 0.06	0.286LysTrp: 0.286 ± 0.017	0.972LysTyr: 0.972 ± 0.033	0.0LysXaa: 0.0 ± 0.0
Leu	12.142LeuAla: 12.142 ± 0.13	1.438LeuCys: 1.438 ± 0.042	6.495LeuAsp: 6.495 ± 0.085	7.135LeuGlu: 7.135 ± 0.106	4.522LeuPhe: 4.522 ± 0.082	8.441LeuGly: 8.441 ± 0.098	2.219LeuHis: 2.219 ± 0.049	5.454LeuIle: 5.454 ± 0.084	4.838LeuLys: 4.838 ± 0.072	14.501LeuLeu: 14.501 ± 0.209	2.12LeuMet: 2.12 ± 0.049	3.244LeuAsn: 3.244 ± 0.05	6.063LeuPro: 6.063 ± 0.089	4.915LeuGln: 4.915 ± 0.089	7.972LeuArg: 7.972 ± 0.096	6.351LeuSer: 6.351 ± 0.077	5.74LeuThr: 5.74 ± 0.081	8.321LeuVal: 8.321 ± 0.084	1.032LeuTrp: 1.032 ± 0.034	2.494LeuTyr: 2.494 ± 0.053	0.0LeuXaa: 0.0 ± 0.0
Met	2.371MetAla: 2.371 ± 0.046	0.17MetCys: 0.17 ± 0.012	1.043MetAsp: 1.043 ± 0.034	1.323MetGlu: 1.323 ± 0.033	0.667MetPhe: 0.667 ± 0.027	1.559MetGly: 1.559 ± 0.043	0.367MetHis: 0.367 ± 0.02	1.213MetIle: 1.213 ± 0.033	1.214MetLys: 1.214 ± 0.035	2.449MetLeu: 2.449 ± 0.05	0.537MetMet: 0.537 ± 0.03	0.864MetAsn: 0.864 ± 0.027	1.138MetPro: 1.138 ± 0.035	0.799MetGln: 0.799 ± 0.028	1.418MetArg: 1.418 ± 0.037	1.288MetSer: 1.288 ± 0.031	1.504MetThr: 1.504 ± 0.037	1.827MetVal: 1.827 ± 0.05	0.135MetTrp: 0.135 ± 0.01	0.34MetTyr: 0.34 ± 0.018	0.0MetXaa: 0.0 ± 0.0
Asn	2.503AsnAla: 2.503 ± 0.057	0.44AsnCys: 0.44 ± 0.021	1.51AsnAsp: 1.51 ± 0.045	1.453AsnGlu: 1.453 ± 0.035	1.177AsnPhe: 1.177 ± 0.034	2.426AsnGly: 2.426 ± 0.076	0.655AsnHis: 0.655 ± 0.026	1.785AsnIle: 1.785 ± 0.039	1.021AsnLys: 1.021 ± 0.032	3.864AsnLeu: 3.864 ± 0.063	0.621AsnMet: 0.621 ± 0.021	1.102AsnAsn: 1.102 ± 0.04	2.059AsnPro: 2.059 ± 0.046	1.09AsnGln: 1.09 ± 0.034	2.529AsnArg: 2.529 ± 0.05	1.497AsnSer: 1.497 ± 0.041	1.259AsnThr: 1.259 ± 0.044	1.792AsnVal: 1.792 ± 0.038	0.421AsnTrp: 0.421 ± 0.022	0.919AsnTyr: 0.919 ± 0.03	0.0AsnXaa: 0.0 ± 0.0
Pro	4.86ProAla: 4.86 ± 0.085	0.512ProCys: 0.512 ± 0.023	3.232ProAsp: 3.232 ± 0.063	4.118ProGlu: 4.118 ± 0.063	1.944ProPhe: 1.944 ± 0.042	4.506ProGly: 4.506 ± 0.081	0.912ProHis: 0.912 ± 0.033	1.985ProIle: 1.985 ± 0.043	1.526ProLys: 1.526 ± 0.044	5.249ProLeu: 5.249 ± 0.077	0.892ProMet: 0.892 ± 0.028	1.154ProAsn: 1.154 ± 0.037	2.266ProPro: 2.266 ± 0.057	1.856ProGln: 1.856 ± 0.044	2.801ProArg: 2.801 ± 0.049	2.255ProSer: 2.255 ± 0.048	2.2ProThr: 2.2 ± 0.048	4.009ProVal: 4.009 ± 0.074	0.602ProTrp: 0.602 ± 0.027	1.146ProTyr: 1.146 ± 0.036	0.0ProXaa: 0.0 ± 0.0
Gln	3.942GlnAla: 3.942 ± 0.064	0.339GlnCys: 0.339 ± 0.019	1.589GlnAsp: 1.589 ± 0.039	2.089GlnGlu: 2.089 ± 0.044	1.254GlnPhe: 1.254 ± 0.034	2.731GlnGly: 2.731 ± 0.049	0.733GlnHis: 0.733 ± 0.023	2.122GlnIle: 2.122 ± 0.048	1.593GlnLys: 1.593 ± 0.042	4.813GlnLeu: 4.813 ± 0.077	0.935GlnMet: 0.935 ± 0.03	1.047GlnAsn: 1.047 ± 0.031	1.912GlnPro: 1.912 ± 0.046	2.366GlnGln: 2.366 ± 0.056	2.941GlnArg: 2.941 ± 0.056	1.746GlnSer: 1.746 ± 0.042	1.78GlnThr: 1.78 ± 0.038	3.129GlnVal: 3.129 ± 0.057	0.377GlnTrp: 0.377 ± 0.02	0.735GlnTyr: 0.735 ± 0.028	0.0GlnXaa: 0.0 ± 0.0
Arg	5.626ArgAla: 5.626 ± 0.081	0.898ArgCys: 0.898 ± 0.034	4.055ArgAsp: 4.055 ± 0.063	5.186ArgGlu: 5.186 ± 0.079	3.077ArgPhe: 3.077 ± 0.059	4.89ArgGly: 4.89 ± 0.075	1.74ArgHis: 1.74 ± 0.04	4.298ArgIle: 4.298 ± 0.066	3.202ArgLys: 3.202 ± 0.055	8.738ArgLeu: 8.738 ± 0.118	1.674ArgMet: 1.674 ± 0.038	2.249ArgAsn: 2.249 ± 0.042	3.187ArgPro: 3.187 ± 0.068	3.887ArgGln: 3.887 ± 0.063	6.257ArgArg: 6.257 ± 0.104	3.635ArgSer: 3.635 ± 0.064	3.077ArgThr: 3.077 ± 0.057	4.926ArgVal: 4.926 ± 0.078	0.838ArgTrp: 0.838 ± 0.029	2.108ArgTyr: 2.108 ± 0.046	0.0ArgXaa: 0.0 ± 0.0
Ser	4.673SerAla: 4.673 ± 0.076	0.848SerCys: 0.848 ± 0.039	2.725SerAsp: 2.725 ± 0.055	2.948SerGlu: 2.948 ± 0.057	2.414SerPhe: 2.414 ± 0.054	5.447SerGly: 5.447 ± 0.091	1.112SerHis: 1.112 ± 0.035	2.732SerIle: 2.732 ± 0.052	1.557SerLys: 1.557 ± 0.045	6.321SerLeu: 6.321 ± 0.079	1.244SerMet: 1.244 ± 0.033	1.582SerAsn: 1.582 ± 0.039	2.664SerPro: 2.664 ± 0.059	1.578SerGln: 1.578 ± 0.042	4.146SerArg: 4.146 ± 0.066	3.163SerSer: 3.163 ± 0.069	2.429SerThr: 2.429 ± 0.056	3.29SerVal: 3.29 ± 0.055	0.736SerTrp: 0.736 ± 0.026	1.549SerTyr: 1.549 ± 0.037	0.0SerXaa: 0.0 ± 0.0
Thr	4.923ThrAla: 4.923 ± 0.082	0.632ThrCys: 0.632 ± 0.038	2.538ThrAsp: 2.538 ± 0.062	2.783ThrGlu: 2.783 ± 0.05	1.841ThrPhe: 1.841 ± 0.037	4.895ThrGly: 4.895 ± 0.092	0.804ThrHis: 0.804 ± 0.027	2.754ThrIle: 2.754 ± 0.052	1.159ThrLys: 1.159 ± 0.032	5.697ThrLeu: 5.697 ± 0.079	1.019ThrMet: 1.019 ± 0.031	1.27ThrAsn: 1.27 ± 0.043	2.872ThrPro: 2.872 ± 0.057	1.031ThrGln: 1.031 ± 0.034	3.412ThrArg: 3.412 ± 0.058	2.553ThrSer: 2.553 ± 0.055	2.704ThrThr: 2.704 ± 0.069	3.725ThrVal: 3.725 ± 0.066	0.499ThrTrp: 0.499 ± 0.023	1.116ThrTyr: 1.116 ± 0.032	0.0ThrXaa: 0.0 ± 0.0
Val	7.186ValAla: 7.186 ± 0.092	0.938ValCys: 0.938 ± 0.034	4.223ValAsp: 4.223 ± 0.066	4.867ValGlu: 4.867 ± 0.077	2.648ValPhe: 2.648 ± 0.048	5.281ValGly: 5.281 ± 0.074	1.271ValHis: 1.271 ± 0.037	3.959ValIle: 3.959 ± 0.062	2.751ValLys: 2.751 ± 0.066	7.523ValLeu: 7.523 ± 0.1	1.539ValMet: 1.539 ± 0.042	2.149ValAsn: 2.149 ± 0.045	3.314ValPro: 3.314 ± 0.06	1.982ValGln: 1.982 ± 0.045	4.833ValArg: 4.833 ± 0.075	4.025ValSer: 4.025 ± 0.06	3.543ValThr: 3.543 ± 0.055	5.591ValVal: 5.591 ± 0.092	0.651ValTrp: 0.651 ± 0.025	1.599ValTyr: 1.599 ± 0.039	0.0ValXaa: 0.0 ± 0.0
Trp	0.731TrpAla: 0.731 ± 0.03	0.145TrpCys: 0.145 ± 0.011	0.587TrpAsp: 0.587 ± 0.025	0.555TrpGlu: 0.555 ± 0.024	0.45TrpPhe: 0.45 ± 0.021	0.787TrpGly: 0.787 ± 0.029	0.256TrpHis: 0.256 ± 0.014	0.54TrpIle: 0.54 ± 0.023	0.435TrpLys: 0.435 ± 0.022	1.407TrpLeu: 1.407 ± 0.045	0.256TrpMet: 0.256 ± 0.016	0.373TrpAsn: 0.373 ± 0.022	0.494TrpPro: 0.494 ± 0.024	0.708TrpGln: 0.708 ± 0.029	0.9TrpArg: 0.9 ± 0.028	0.639TrpSer: 0.639 ± 0.029	0.511TrpThr: 0.511 ± 0.027	0.641TrpVal: 0.641 ± 0.025	0.174TrpTrp: 0.174 ± 0.012	0.284TrpTyr: 0.284 ± 0.015	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.202TyrAla: 2.202 ± 0.044	0.367TyrCys: 0.367 ± 0.018	1.414TyrAsp: 1.414 ± 0.041	1.242TyrGlu: 1.242 ± 0.04	1.109TyrPhe: 1.109 ± 0.036	2.099TyrGly: 2.099 ± 0.042	0.639TyrHis: 0.639 ± 0.023	1.1TyrIle: 1.1 ± 0.032	0.794TyrLys: 0.794 ± 0.033	3.308TyrLeu: 3.308 ± 0.063	0.384TyrMet: 0.384 ± 0.019	0.761TyrAsn: 0.761 ± 0.03	1.351TyrPro: 1.351 ± 0.037	1.212TyrGln: 1.212 ± 0.033	2.592TyrArg: 2.592 ± 0.046	1.38TyrSer: 1.38 ± 0.038	1.073TyrThr: 1.073 ± 0.041	1.416TyrVal: 1.416 ± 0.039	0.304TyrTrp: 0.304 ± 0.019	0.816TyrTyr: 0.816 ± 0.032	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 3263 proteins (1086227 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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