Raccoon-associated polyomavirus 2

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cossaviricota; Papovaviricetes; Sepolyvirales; Polyomaviridae; Deltapolyomavirus; Procyon lotor polyomavirus 2

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1W5X9W6|A0A1W5X9W6_9POLY MT OS=Raccoon-associated polyomavirus 2 OX=1978920 PE=4 SV=1
MM1 pKa = 7.37GGVLSVLVDD10 pKa = 5.05LIQLLPEE17 pKa = 4.7LSASTGFGIEE27 pKa = 4.44AILAGEE33 pKa = 4.15AAASVEE39 pKa = 4.1AQVTSLMLVEE49 pKa = 4.74SLGPLDD55 pKa = 4.78ALASLGLSAEE65 pKa = 4.32SFSLLSAMPGFFQDD79 pKa = 3.73VVGLGVLFQTVSGASSLVAAGIQLRR104 pKa = 11.84HH105 pKa = 5.64EE106 pKa = 4.32VSVVNRR112 pKa = 11.84NMALAPWIPHH122 pKa = 6.69DD123 pKa = 4.9LYY125 pKa = 11.08DD126 pKa = 3.46IYY128 pKa = 11.13FPGVSTFSYY137 pKa = 9.25VVNVITDD144 pKa = 3.31WGISLFHH151 pKa = 6.61TIARR155 pKa = 11.84NVWDD159 pKa = 3.61EE160 pKa = 3.76LVRR163 pKa = 11.84EE164 pKa = 4.21GRR166 pKa = 11.84EE167 pKa = 3.74QIEE170 pKa = 4.15QVTRR174 pKa = 11.84DD175 pKa = 3.18VALRR179 pKa = 11.84GVQTFADD186 pKa = 3.68TMARR190 pKa = 11.84LIEE193 pKa = 4.03RR194 pKa = 11.84ARR196 pKa = 11.84WTITNSPIHH205 pKa = 5.68HH206 pKa = 7.09AYY208 pKa = 9.13WYY210 pKa = 10.99LEE212 pKa = 4.2DD213 pKa = 3.95YY214 pKa = 10.66YY215 pKa = 11.65KK216 pKa = 10.68RR217 pKa = 11.84LPQINPPQARR227 pKa = 11.84QLFRR231 pKa = 11.84RR232 pKa = 11.84IGQRR236 pKa = 11.84PPDD239 pKa = 3.61RR240 pKa = 11.84SNLEE244 pKa = 3.92EE245 pKa = 4.24LEE247 pKa = 4.19KK248 pKa = 10.74EE249 pKa = 4.09SRR251 pKa = 11.84EE252 pKa = 3.8EE253 pKa = 3.59SGQIVEE259 pKa = 4.76KK260 pKa = 10.97YY261 pKa = 8.77EE262 pKa = 4.03PPGGAFQRR270 pKa = 11.84VTPDD274 pKa = 2.31WMLPLILGLYY284 pKa = 10.53GDD286 pKa = 4.64ITPTWSTYY294 pKa = 10.0IEE296 pKa = 4.12QIEE299 pKa = 4.23AEE301 pKa = 3.95EE302 pKa = 4.83DD303 pKa = 3.81GPKK306 pKa = 10.06KK307 pKa = 10.09KK308 pKa = 9.98RR309 pKa = 11.84RR310 pKa = 11.84RR311 pKa = 11.84QQ312 pKa = 3.19

Molecular weight: 34.84 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1W5X9X0|A0A1W5X9X0_9POLY ALTO OS=Raccoon-associated polyomavirus 2 OX=1978920 PE=4 SV=1
MM1 pKa = 8.0RR2 pKa = 11.84NTSLHH7 pKa = 6.26PGVPEE12 pKa = 4.0APEE15 pKa = 4.17PLLLSLVLLLLPLPLLPHH33 pKa = 6.91PLLLQLILLLLLHH46 pKa = 6.91LGGLHH51 pKa = 6.75LCCIPPHH58 pKa = 6.39RR59 pKa = 11.84RR60 pKa = 11.84PPTTTRR66 pKa = 11.84EE67 pKa = 3.69RR68 pKa = 11.84SHH70 pKa = 6.5RR71 pKa = 11.84HH72 pKa = 4.38QLPSRR77 pKa = 11.84ASPHH81 pKa = 5.37PRR83 pKa = 11.84SRR85 pKa = 11.84RR86 pKa = 11.84KK87 pKa = 8.88WMHH90 pKa = 5.89HH91 pKa = 4.94

Molecular weight: 10.44 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7

0

7

2221

91

717

317.3

35.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.178 ± 0.527

2.296 ± 0.706

4.683 ± 0.444

6.529 ± 0.448

3.647 ± 0.368

5.583 ± 0.707

2.341 ± 0.662

3.917 ± 0.707

6.123 ± 1.178

11.571 ± 1.81

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.701 ± 0.248

3.332 ± 0.426

8.915 ± 1.103

4.728 ± 0.199

5.313 ± 0.872

7.564 ± 0.447

5.133 ± 0.644

5.853 ± 0.769

1.441 ± 0.35

3.152 ± 0.29
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski