Gordonia phage Dardanus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Vendettavirus; unclassified Vendettavirus

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A514CX43|A0A514CX43_9CAUD Uncharacterized protein OS=Gordonia phage Dardanus OX=2588489 GN=47 PE=4 SV=1
MM1 pKa = 7.56SNHH4 pKa = 5.83TNGAVTIPGEE14 pKa = 4.1IVPTILTLCVAAIEE28 pKa = 4.14QLHH31 pKa = 6.76DD32 pKa = 3.47EE33 pKa = 4.53VEE35 pKa = 4.23RR36 pKa = 11.84LTAANVEE43 pKa = 4.47MADD46 pKa = 3.67EE47 pKa = 4.61LADD50 pKa = 3.67EE51 pKa = 4.84DD52 pKa = 4.82AAAEE56 pKa = 4.58LPDD59 pKa = 4.47EE60 pKa = 4.99PDD62 pKa = 3.01AGAFGLYY69 pKa = 9.99ADD71 pKa = 4.65RR72 pKa = 11.84AGDD75 pKa = 3.43VWRR78 pKa = 11.84YY79 pKa = 9.8DD80 pKa = 3.29ADD82 pKa = 3.04GWQPIEE88 pKa = 4.12TSDD91 pKa = 4.19GVDD94 pKa = 3.38VTHH97 pKa = 6.8LTFATEE103 pKa = 3.99WCDD106 pKa = 3.88SVADD110 pKa = 4.05CGPFVRR116 pKa = 11.84LTGYY120 pKa = 10.62KK121 pKa = 9.85LASEE125 pKa = 4.37EE126 pKa = 4.15SGEE129 pKa = 4.22VEE131 pKa = 4.05NN132 pKa = 5.73

Molecular weight:
14.17 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A514CX33|A0A514CX33_9CAUD Tyrosine integrase OS=Gordonia phage Dardanus OX=2588489 GN=35 PE=3 SV=1
MM1 pKa = 7.15SAEE4 pKa = 4.13GVDD7 pKa = 4.21LEE9 pKa = 4.53TVDD12 pKa = 4.77FSRR15 pKa = 11.84HH16 pKa = 3.54ATQRR20 pKa = 11.84ALDD23 pKa = 3.81MAVGADD29 pKa = 4.06EE30 pKa = 4.15IRR32 pKa = 11.84ACIEE36 pKa = 3.71RR37 pKa = 11.84PAEE40 pKa = 4.12TRR42 pKa = 11.84RR43 pKa = 11.84LGYY46 pKa = 9.68PRR48 pKa = 11.84GDD50 pKa = 3.2GTRR53 pKa = 11.84RR54 pKa = 11.84WKK56 pKa = 9.15FTRR59 pKa = 11.84GRR61 pKa = 11.84LAVVMQSEE69 pKa = 4.98SNGRR73 pKa = 11.84WTAVTVLWSRR83 pKa = 11.84EE84 pKa = 3.62GLFRR88 pKa = 11.84NDD90 pKa = 2.87FAMHH94 pKa = 6.49GEE96 pKa = 4.22YY97 pKa = 10.21EE98 pKa = 4.0GRR100 pKa = 11.84TRR102 pKa = 11.84KK103 pKa = 9.87SRR105 pKa = 11.84TRR107 pKa = 11.84AATLIRR113 pKa = 11.84HH114 pKa = 6.05

Molecular weight:
13.07 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

72

0

72

13392

46

1196

186.0

20.18

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.888 ± 0.554

0.694 ± 0.113

6.847 ± 0.232

6.28 ± 0.269

3.062 ± 0.177

9.692 ± 0.467

1.971 ± 0.208

3.539 ± 0.185

3.666 ± 0.216

7.445 ± 0.273

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.151 ± 0.119

3.076 ± 0.18

4.645 ± 0.244

3.151 ± 0.175

7.646 ± 0.387

4.973 ± 0.202

5.795 ± 0.298

8.027 ± 0.292

1.956 ± 0.154

2.494 ± 0.19

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski