Gordonia phage Dardanus
Average proteome isoelectric point is 6.41
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 72 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A514CX43|A0A514CX43_9CAUD Uncharacterized protein OS=Gordonia phage Dardanus OX=2588489 GN=47 PE=4 SV=1
MM1 pKa = 7.56 SNHH4 pKa = 5.83 TNGAVTIPGEE14 pKa = 4.1 IVPTILTLCVAAIEE28 pKa = 4.14 QLHH31 pKa = 6.76 DD32 pKa = 3.47 EE33 pKa = 4.53 VEE35 pKa = 4.23 RR36 pKa = 11.84 LTAANVEE43 pKa = 4.47 MADD46 pKa = 3.67 EE47 pKa = 4.61 LADD50 pKa = 3.67 EE51 pKa = 4.84 DD52 pKa = 4.82 AAAEE56 pKa = 4.58 LPDD59 pKa = 4.47 EE60 pKa = 4.99 PDD62 pKa = 3.01 AGAFGLYY69 pKa = 9.99 ADD71 pKa = 4.65 RR72 pKa = 11.84 AGDD75 pKa = 3.43 VWRR78 pKa = 11.84 YY79 pKa = 9.8 DD80 pKa = 3.29 ADD82 pKa = 3.04 GWQPIEE88 pKa = 4.12 TSDD91 pKa = 4.19 GVDD94 pKa = 3.38 VTHH97 pKa = 6.8 LTFATEE103 pKa = 3.99 WCDD106 pKa = 3.88 SVADD110 pKa = 4.05 CGPFVRR116 pKa = 11.84 LTGYY120 pKa = 10.62 KK121 pKa = 9.85 LASEE125 pKa = 4.37 EE126 pKa = 4.15 SGEE129 pKa = 4.22 VEE131 pKa = 4.05 NN132 pKa = 5.73
Molecular weight: 14.17 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.756
IPC2_protein 3.732
IPC_protein 3.706
Toseland 3.503
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.846
Wikipedia 3.605
Rodwell 3.528
Grimsley 3.414
Solomon 3.668
Lehninger 3.63
Nozaki 3.808
DTASelect 3.986
Thurlkill 3.554
EMBOSS 3.617
Sillero 3.821
Patrickios 1.837
IPC_peptide 3.668
IPC2_peptide 3.795
IPC2.peptide.svr19 3.753
Protein with the highest isoelectric point:
>tr|A0A514CX33|A0A514CX33_9CAUD Tyrosine integrase OS=Gordonia phage Dardanus OX=2588489 GN=35 PE=3 SV=1
MM1 pKa = 7.15 SAEE4 pKa = 4.13 GVDD7 pKa = 4.21 LEE9 pKa = 4.53 TVDD12 pKa = 4.77 FSRR15 pKa = 11.84 HH16 pKa = 3.54 ATQRR20 pKa = 11.84 ALDD23 pKa = 3.81 MAVGADD29 pKa = 4.06 EE30 pKa = 4.15 IRR32 pKa = 11.84 ACIEE36 pKa = 3.71 RR37 pKa = 11.84 PAEE40 pKa = 4.12 TRR42 pKa = 11.84 RR43 pKa = 11.84 LGYY46 pKa = 9.68 PRR48 pKa = 11.84 GDD50 pKa = 3.2 GTRR53 pKa = 11.84 RR54 pKa = 11.84 WKK56 pKa = 9.15 FTRR59 pKa = 11.84 GRR61 pKa = 11.84 LAVVMQSEE69 pKa = 4.98 SNGRR73 pKa = 11.84 WTAVTVLWSRR83 pKa = 11.84 EE84 pKa = 3.62 GLFRR88 pKa = 11.84 NDD90 pKa = 2.87 FAMHH94 pKa = 6.49 GEE96 pKa = 4.22 YY97 pKa = 10.21 EE98 pKa = 4.0 GRR100 pKa = 11.84 TRR102 pKa = 11.84 KK103 pKa = 9.87 SRR105 pKa = 11.84 TRR107 pKa = 11.84 AATLIRR113 pKa = 11.84 HH114 pKa = 6.05
Molecular weight: 13.07 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.325
IPC2_protein 9.458
IPC_protein 10.687
Toseland 10.687
ProMoST 10.804
Dawson 10.774
Bjellqvist 10.628
Wikipedia 11.111
Rodwell 10.613
Grimsley 10.833
Solomon 11.067
Lehninger 10.994
Nozaki 10.687
DTASelect 10.628
Thurlkill 10.701
EMBOSS 11.14
Sillero 10.73
Patrickios 10.394
IPC_peptide 11.067
IPC2_peptide 9.97
IPC2.peptide.svr19 8.806
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
72
0
72
13392
46
1196
186.0
20.18
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.888 ± 0.554
0.694 ± 0.113
6.847 ± 0.232
6.28 ± 0.269
3.062 ± 0.177
9.692 ± 0.467
1.971 ± 0.208
3.539 ± 0.185
3.666 ± 0.216
7.445 ± 0.273
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.151 ± 0.119
3.076 ± 0.18
4.645 ± 0.244
3.151 ± 0.175
7.646 ± 0.387
4.973 ± 0.202
5.795 ± 0.298
8.027 ± 0.292
1.956 ± 0.154
2.494 ± 0.19
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here