Amino acid dipepetide frequency for Streptomyces sp. BA2

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
20.451AlaAla: 20.451 ± 0.114
1.09AlaCys: 1.09 ± 0.021
8.345AlaAsp: 8.345 ± 0.057
8.61AlaGlu: 8.61 ± 0.072
3.52AlaPhe: 3.52 ± 0.038
12.526AlaGly: 12.526 ± 0.084
2.828AlaHis: 2.828 ± 0.041
3.761AlaIle: 3.761 ± 0.042
3.273AlaLys: 3.273 ± 0.05
14.29AlaLeu: 14.29 ± 0.096
2.587AlaMet: 2.587 ± 0.034
1.924AlaAsn: 1.924 ± 0.031
7.022AlaPro: 7.022 ± 0.063
3.817AlaGln: 3.817 ± 0.04
9.55AlaArg: 9.55 ± 0.082
6.238AlaSer: 6.238 ± 0.052
6.928AlaThr: 6.928 ± 0.06
11.839AlaVal: 11.839 ± 0.077
1.909AlaTrp: 1.909 ± 0.028
2.894AlaTyr: 2.894 ± 0.032
0.0AlaXaa: 0.0 ± 0.0
Cys
1.063CysAla: 1.063 ± 0.019
0.089CysCys: 0.089 ± 0.006
0.471CysAsp: 0.471 ± 0.013
0.416CysGlu: 0.416 ± 0.013
0.217CysPhe: 0.217 ± 0.009
0.987CysGly: 0.987 ± 0.021
0.195CysHis: 0.195 ± 0.008
0.178CysIle: 0.178 ± 0.007
0.125CysLys: 0.125 ± 0.007
0.744CysLeu: 0.744 ± 0.016
0.139CysMet: 0.139 ± 0.007
0.127CysAsn: 0.127 ± 0.007
0.479CysPro: 0.479 ± 0.014
0.179CysGln: 0.179 ± 0.008
0.587CysArg: 0.587 ± 0.015
0.424CysSer: 0.424 ± 0.013
0.507CysThr: 0.507 ± 0.013
0.696CysVal: 0.696 ± 0.017
0.126CysTrp: 0.126 ± 0.007
0.158CysTyr: 0.158 ± 0.007
0.0CysXaa: 0.0 ± 0.0
Asp
7.778AspAla: 7.778 ± 0.066
0.437AspCys: 0.437 ± 0.014
3.741AspAsp: 3.741 ± 0.046
4.018AspGlu: 4.018 ± 0.043
1.708AspPhe: 1.708 ± 0.028
6.293AspGly: 6.293 ± 0.053
1.411AspHis: 1.411 ± 0.022
2.026AspIle: 2.026 ± 0.027
1.516AspLys: 1.516 ± 0.028
6.067AspLeu: 6.067 ± 0.052
0.909AspMet: 0.909 ± 0.016
1.005AspAsn: 1.005 ± 0.018
4.321AspPro: 4.321 ± 0.05
1.57AspGln: 1.57 ± 0.024
4.576AspArg: 4.576 ± 0.042
2.726AspSer: 2.726 ± 0.031
3.179AspThr: 3.179 ± 0.039
4.765AspVal: 4.765 ± 0.045
1.012AspTrp: 1.012 ± 0.016
1.134AspTyr: 1.134 ± 0.02
0.0AspXaa: 0.0 ± 0.0
Glu
7.39GluAla: 7.39 ± 0.067
0.377GluCys: 0.377 ± 0.011
2.758GluAsp: 2.758 ± 0.034
3.467GluGlu: 3.467 ± 0.043
1.514GluPhe: 1.514 ± 0.026
4.622GluGly: 4.622 ± 0.048
1.485GluHis: 1.485 ± 0.027
2.274GluIle: 2.274 ± 0.03
1.67GluLys: 1.67 ± 0.032
6.931GluLeu: 6.931 ± 0.066
0.92GluMet: 0.92 ± 0.02
1.053GluAsn: 1.053 ± 0.019
3.376GluPro: 3.376 ± 0.039
2.366GluGln: 2.366 ± 0.031
5.519GluArg: 5.519 ± 0.052
2.773GluSer: 2.773 ± 0.03
2.91GluThr: 2.91 ± 0.034
4.302GluVal: 4.302 ± 0.045
0.782GluTrp: 0.782 ± 0.019
1.137GluTyr: 1.137 ± 0.02
0.0GluXaa: 0.0 ± 0.0
Phe
3.704PheAla: 3.704 ± 0.043
0.258PheCys: 0.258 ± 0.01
1.967PheAsp: 1.967 ± 0.029
1.434PheGlu: 1.434 ± 0.022
0.899PhePhe: 0.899 ± 0.021
3.106PheGly: 3.106 ± 0.042
0.612PheHis: 0.612 ± 0.017
0.841PheIle: 0.841 ± 0.018
0.622PheLys: 0.622 ± 0.015
2.597PheLeu: 2.597 ± 0.035
0.448PheMet: 0.448 ± 0.013
0.608PheAsn: 0.608 ± 0.016
1.387PhePro: 1.387 ± 0.022
0.702PheGln: 0.702 ± 0.016
1.79PheArg: 1.79 ± 0.028
1.539PheSer: 1.539 ± 0.026
2.021PheThr: 2.021 ± 0.028
2.233PheVal: 2.233 ± 0.03
0.426PheTrp: 0.426 ± 0.012
0.595PheTyr: 0.595 ± 0.018
0.0PheXaa: 0.0 ± 0.0
Gly
11.117GlyAla: 11.117 ± 0.076
0.804GlyCys: 0.804 ± 0.018
5.363GlyAsp: 5.363 ± 0.056
5.341GlyGlu: 5.341 ± 0.048
2.901GlyPhe: 2.901 ± 0.037
9.01GlyGly: 9.01 ± 0.106
2.326GlyHis: 2.326 ± 0.03
3.592GlyIle: 3.592 ± 0.045
2.895GlyLys: 2.895 ± 0.044
9.453GlyLeu: 9.453 ± 0.061
2.043GlyMet: 2.043 ± 0.029
1.826GlyAsn: 1.826 ± 0.03
4.92GlyPro: 4.92 ± 0.044
2.789GlyGln: 2.789 ± 0.036
7.144GlyArg: 7.144 ± 0.057
5.666GlySer: 5.666 ± 0.053
6.052GlyThr: 6.052 ± 0.048
7.404GlyVal: 7.404 ± 0.055
1.699GlyTrp: 1.699 ± 0.025
2.266GlyTyr: 2.266 ± 0.033
0.0GlyXaa: 0.0 ± 0.0
His
2.745HisAla: 2.745 ± 0.037
0.226HisCys: 0.226 ± 0.009
1.341HisAsp: 1.341 ± 0.023
1.252HisGlu: 1.252 ± 0.021
0.653HisPhe: 0.653 ± 0.016
2.356HisGly: 2.356 ± 0.032
0.7HisHis: 0.7 ± 0.025
0.735HisIle: 0.735 ± 0.017
0.418HisLys: 0.418 ± 0.01
2.379HisLeu: 2.379 ± 0.037
0.35HisMet: 0.35 ± 0.012
0.389HisAsn: 0.389 ± 0.013
1.794HisPro: 1.794 ± 0.03
0.7HisGln: 0.7 ± 0.018
2.013HisArg: 2.013 ± 0.03
1.063HisSer: 1.063 ± 0.021
1.364HisThr: 1.364 ± 0.033
1.7HisVal: 1.7 ± 0.027
0.367HisTrp: 0.367 ± 0.01
0.492HisTyr: 0.492 ± 0.014
0.0HisXaa: 0.0 ± 0.0
Ile
5.081IleAla: 5.081 ± 0.049
0.311IleCys: 0.311 ± 0.012
2.348IleAsp: 2.348 ± 0.029
2.079IleGlu: 2.079 ± 0.029
0.775IlePhe: 0.775 ± 0.016
3.829IleGly: 3.829 ± 0.048
0.651IleHis: 0.651 ± 0.017
0.945IleIle: 0.945 ± 0.023
0.88IleLys: 0.88 ± 0.02
2.587IleLeu: 2.587 ± 0.033
0.455IleMet: 0.455 ± 0.013
0.753IleAsn: 0.753 ± 0.016
1.874IlePro: 1.874 ± 0.027
0.756IleGln: 0.756 ± 0.016
2.188IleArg: 2.188 ± 0.028
1.855IleSer: 1.855 ± 0.028
2.375IleThr: 2.375 ± 0.029
2.765IleVal: 2.765 ± 0.038
0.401IleTrp: 0.401 ± 0.013
0.586IleTyr: 0.586 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
3.363LysAla: 3.363 ± 0.049
0.135LysCys: 0.135 ± 0.007
1.58LysAsp: 1.58 ± 0.031
1.443LysGlu: 1.443 ± 0.026
0.571LysPhe: 0.571 ± 0.016
2.225LysGly: 2.225 ± 0.035
0.501LysHis: 0.501 ± 0.014
0.979LysIle: 0.979 ± 0.02
1.209LysLys: 1.209 ± 0.031
2.28LysLeu: 2.28 ± 0.033
0.472LysMet: 0.472 ± 0.013
0.616LysAsn: 0.616 ± 0.016
1.619LysPro: 1.619 ± 0.031
0.801LysGln: 0.801 ± 0.018
1.601LysArg: 1.601 ± 0.027
1.46LysSer: 1.46 ± 0.026
1.487LysThr: 1.487 ± 0.026
2.118LysVal: 2.118 ± 0.036
0.335LysTrp: 0.335 ± 0.011
0.53LysTyr: 0.53 ± 0.015
0.0LysXaa: 0.0 ± 0.0
Leu
14.706LeuAla: 14.706 ± 0.096
0.872LeuCys: 0.872 ± 0.021
6.544LeuAsp: 6.544 ± 0.058
4.531LeuGlu: 4.531 ± 0.044
2.727LeuPhe: 2.727 ± 0.039
9.242LeuGly: 9.242 ± 0.061
2.301LeuHis: 2.301 ± 0.035
3.586LeuIle: 3.586 ± 0.04
2.279LeuLys: 2.279 ± 0.03
11.082LeuLeu: 11.082 ± 0.084
1.752LeuMet: 1.752 ± 0.023
1.777LeuAsn: 1.777 ± 0.024
6.444LeuPro: 6.444 ± 0.057
2.208LeuGln: 2.208 ± 0.029
8.354LeuArg: 8.354 ± 0.069
5.55LeuSer: 5.55 ± 0.046
6.926LeuThr: 6.926 ± 0.068
8.474LeuVal: 8.474 ± 0.065
1.286LeuTrp: 1.286 ± 0.023
1.832LeuTyr: 1.832 ± 0.026
0.0LeuXaa: 0.0 ± 0.0
Met
2.386MetAla: 2.386 ± 0.031
0.147MetCys: 0.147 ± 0.008
0.961MetAsp: 0.961 ± 0.018
0.818MetGlu: 0.818 ± 0.018
0.47MetPhe: 0.47 ± 0.015
1.396MetGly: 1.396 ± 0.024
0.389MetHis: 0.389 ± 0.012
0.678MetIle: 0.678 ± 0.018
0.485MetLys: 0.485 ± 0.014
1.722MetLeu: 1.722 ± 0.025
0.319MetMet: 0.319 ± 0.011
0.471MetAsn: 0.471 ± 0.013
1.193MetPro: 1.193 ± 0.023
0.464MetGln: 0.464 ± 0.014
1.497MetArg: 1.497 ± 0.023
1.35MetSer: 1.35 ± 0.021
1.538MetThr: 1.538 ± 0.025
1.3MetVal: 1.3 ± 0.021
0.222MetTrp: 0.222 ± 0.009
0.348MetTyr: 0.348 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.229AsnAla: 2.229 ± 0.031
0.168AsnCys: 0.168 ± 0.009
1.002AsnAsp: 1.002 ± 0.018
0.836AsnGlu: 0.836 ± 0.015
0.507AsnPhe: 0.507 ± 0.015
1.933AsnGly: 1.933 ± 0.028
0.397AsnHis: 0.397 ± 0.012
0.684AsnIle: 0.684 ± 0.016
0.492AsnLys: 0.492 ± 0.014
1.7AsnLeu: 1.7 ± 0.025
0.303AsnMet: 0.303 ± 0.01
0.442AsnAsn: 0.442 ± 0.014
1.41AsnPro: 1.41 ± 0.023
0.54AsnGln: 0.54 ± 0.014
1.275AsnArg: 1.275 ± 0.025
1.055AsnSer: 1.055 ± 0.02
1.135AsnThr: 1.135 ± 0.024
1.385AsnVal: 1.385 ± 0.023
0.316AsnTrp: 0.316 ± 0.011
0.423AsnTyr: 0.423 ± 0.012
0.0AsnXaa: 0.0 ± 0.0
Pro
7.977ProAla: 7.977 ± 0.071
0.346ProCys: 0.346 ± 0.011
4.415ProAsp: 4.415 ± 0.048
4.334ProGlu: 4.334 ± 0.046
1.528ProPhe: 1.528 ± 0.022
6.359ProGly: 6.359 ± 0.055
1.436ProHis: 1.436 ± 0.025
1.371ProIle: 1.371 ± 0.023
1.438ProLys: 1.438 ± 0.025
5.366ProLeu: 5.366 ± 0.052
1.036ProMet: 1.036 ± 0.021
0.952ProAsn: 0.952 ± 0.022
3.461ProPro: 3.461 ± 0.054
1.924ProGln: 1.924 ± 0.039
3.811ProArg: 3.811 ± 0.041
3.323ProSer: 3.323 ± 0.042
3.277ProThr: 3.277 ± 0.04
5.183ProVal: 5.183 ± 0.044
0.896ProTrp: 0.896 ± 0.018
1.389ProTyr: 1.389 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.762GlnAla: 3.762 ± 0.037
0.171GlnCys: 0.171 ± 0.007
1.441GlnAsp: 1.441 ± 0.023
1.549GlnGlu: 1.549 ± 0.026
0.723GlnPhe: 0.723 ± 0.015
2.434GlnGly: 2.434 ± 0.033
0.689GlnHis: 0.689 ± 0.015
1.094GlnIle: 1.094 ± 0.019
0.7GlnLys: 0.7 ± 0.016
3.338GlnLeu: 3.338 ± 0.043
0.534GlnMet: 0.534 ± 0.014
0.533GlnAsn: 0.533 ± 0.012
1.783GlnPro: 1.783 ± 0.035
1.305GlnGln: 1.305 ± 0.033
2.425GlnArg: 2.425 ± 0.033
1.361GlnSer: 1.361 ± 0.024
1.368GlnThr: 1.368 ± 0.025
2.302GlnVal: 2.302 ± 0.03
0.503GlnTrp: 0.503 ± 0.012
0.618GlnTyr: 0.618 ± 0.014
0.0GlnXaa: 0.0 ± 0.0
Arg
9.249ArgAla: 9.249 ± 0.074
0.572ArgCys: 0.572 ± 0.016
4.161ArgAsp: 4.161 ± 0.043
4.717ArgGlu: 4.717 ± 0.041
2.238ArgPhe: 2.238 ± 0.031
5.737ArgGly: 5.737 ± 0.055
2.102ArgHis: 2.102 ± 0.032
3.192ArgIle: 3.192 ± 0.034
1.83ArgLys: 1.83 ± 0.028
8.146ArgLeu: 8.146 ± 0.071
1.736ArgMet: 1.736 ± 0.026
1.326ArgAsn: 1.326 ± 0.025
4.539ArgPro: 4.539 ± 0.046
2.327ArgGln: 2.327 ± 0.028
7.008ArgArg: 7.008 ± 0.073
3.913ArgSer: 3.913 ± 0.042
5.175ArgThr: 5.175 ± 0.051
5.549ArgVal: 5.549 ± 0.051
1.344ArgTrp: 1.344 ± 0.022
1.729ArgTyr: 1.729 ± 0.03
0.0ArgXaa: 0.0 ± 0.0
Ser
6.927SerAla: 6.927 ± 0.051
0.421SerCys: 0.421 ± 0.012
2.894SerAsp: 2.894 ± 0.034
2.669SerGlu: 2.669 ± 0.032
1.616SerPhe: 1.616 ± 0.025
6.219SerGly: 6.219 ± 0.059
1.091SerHis: 1.091 ± 0.02
1.58SerIle: 1.58 ± 0.027
1.301SerLys: 1.301 ± 0.026
5.036SerLeu: 5.036 ± 0.042
1.141SerMet: 1.141 ± 0.019
0.933SerAsn: 0.933 ± 0.019
3.266SerPro: 3.266 ± 0.043
1.37SerGln: 1.37 ± 0.025
3.657SerArg: 3.657 ± 0.036
3.037SerSer: 3.037 ± 0.043
3.222SerThr: 3.222 ± 0.037
4.328SerVal: 4.328 ± 0.042
0.944SerTrp: 0.944 ± 0.021
1.298SerTyr: 1.298 ± 0.021
0.0SerXaa: 0.0 ± 0.0
Thr
8.57ThrAla: 8.57 ± 0.055
0.407ThrCys: 0.407 ± 0.012
3.608ThrAsp: 3.608 ± 0.039
3.344ThrGlu: 3.344 ± 0.034
1.653ThrPhe: 1.653 ± 0.028
6.458ThrGly: 6.458 ± 0.047
1.279ThrHis: 1.279 ± 0.028
1.8ThrIle: 1.8 ± 0.033
1.378ThrLys: 1.378 ± 0.026
5.837ThrLeu: 5.837 ± 0.055
0.975ThrMet: 0.975 ± 0.021
1.035ThrAsn: 1.035 ± 0.021
4.16ThrPro: 4.16 ± 0.043
1.456ThrGln: 1.456 ± 0.024
3.746ThrArg: 3.746 ± 0.039
3.261ThrSer: 3.261 ± 0.041
3.769ThrThr: 3.769 ± 0.048
5.785ThrVal: 5.785 ± 0.048
0.897ThrTrp: 0.897 ± 0.021
1.45ThrTyr: 1.45 ± 0.028
0.0ThrXaa: 0.0 ± 0.0
Val
10.343ValAla: 10.343 ± 0.065
0.734ValCys: 0.734 ± 0.019
4.89ValAsp: 4.89 ± 0.045
4.652ValGlu: 4.652 ± 0.052
2.384ValPhe: 2.384 ± 0.028
6.466ValGly: 6.466 ± 0.056
1.889ValHis: 1.889 ± 0.027
3.072ValIle: 3.072 ± 0.036
1.932ValLys: 1.932 ± 0.033
9.1ValLeu: 9.1 ± 0.069
1.45ValMet: 1.45 ± 0.024
1.629ValAsn: 1.629 ± 0.026
4.944ValPro: 4.944 ± 0.047
2.107ValGln: 2.107 ± 0.031
6.775ValArg: 6.775 ± 0.055
4.384ValSer: 4.384 ± 0.039
5.444ValThr: 5.444 ± 0.049
7.512ValVal: 7.512 ± 0.07
1.147ValTrp: 1.147 ± 0.019
1.506ValTyr: 1.506 ± 0.024
0.0ValXaa: 0.0 ± 0.0
Trp
1.693TrpAla: 1.693 ± 0.027
0.166TrpCys: 0.166 ± 0.008
0.852TrpAsp: 0.852 ± 0.019
0.736TrpGlu: 0.736 ± 0.017
0.516TrpPhe: 0.516 ± 0.014
1.117TrpGly: 1.117 ± 0.021
0.376TrpHis: 0.376 ± 0.012
0.588TrpIle: 0.588 ± 0.016
0.399TrpLys: 0.399 ± 0.013
1.787TrpLeu: 1.787 ± 0.03
0.31TrpMet: 0.31 ± 0.01
0.416TrpAsn: 0.416 ± 0.012
0.776TrpPro: 0.776 ± 0.016
0.649TrpGln: 0.649 ± 0.015
1.292TrpArg: 1.292 ± 0.024
0.97TrpSer: 0.97 ± 0.021
0.996TrpThr: 0.996 ± 0.02
0.988TrpVal: 0.988 ± 0.02
0.35TrpTrp: 0.35 ± 0.012
0.357TrpTyr: 0.357 ± 0.013
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.87TyrAla: 2.87 ± 0.034
0.177TyrCys: 0.177 ± 0.007
1.507TyrAsp: 1.507 ± 0.031
1.381TyrGlu: 1.381 ± 0.022
0.669TyrPhe: 0.669 ± 0.018
2.297TyrGly: 2.297 ± 0.031
0.378TyrHis: 0.378 ± 0.013
0.518TyrIle: 0.518 ± 0.014
0.48TyrLys: 0.48 ± 0.014
2.03TyrLeu: 2.03 ± 0.027
0.277TyrMet: 0.277 ± 0.011
0.418TyrAsn: 0.418 ± 0.015
1.054TyrPro: 1.054 ± 0.019
0.601TyrGln: 0.601 ± 0.016
1.782TyrArg: 1.782 ± 0.026
1.002TyrSer: 1.002 ± 0.02
1.194TyrThr: 1.194 ± 0.022
1.732TyrVal: 1.732 ± 0.025
0.372TyrTrp: 0.372 ± 0.012
0.461TyrTyr: 0.461 ± 0.014
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 8618 proteins (2842209 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski