Escherichia phage alpha3 (Bacteriophage alpha-3)

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; Bullavirinae; Alphatrevirus; Escherichia virus alpha3

Average proteome isoelectric point is 7.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P08767|CAPSD_BPAL3 Capsid protein F OS=Escherichia phage alpha3 OX=10849 GN=F PE=1 SV=2
MM1 pKa = 7.44NIVSDD6 pKa = 3.95VNYY9 pKa = 7.9ATSVAALRR17 pKa = 11.84MLQASAVLDD26 pKa = 3.66VTEE29 pKa = 4.69EE30 pKa = 4.15DD31 pKa = 3.93FDD33 pKa = 4.81FLTGDD38 pKa = 4.49KK39 pKa = 10.3IWIATDD45 pKa = 3.1RR46 pKa = 11.84NRR48 pKa = 11.84ARR50 pKa = 11.84RR51 pKa = 11.84CVEE54 pKa = 3.39ACVYY58 pKa = 8.38GTLDD62 pKa = 3.27FVGYY66 pKa = 9.24PRR68 pKa = 11.84FPAPVEE74 pKa = 4.43FIAAVIAYY82 pKa = 7.67YY83 pKa = 8.75VHH85 pKa = 6.81PVNVQTACLVMEE97 pKa = 4.47GAEE100 pKa = 3.93FSEE103 pKa = 4.43NIINGVEE110 pKa = 4.06RR111 pKa = 11.84PVNAAEE117 pKa = 3.99LFAYY121 pKa = 7.64TLRR124 pKa = 11.84IKK126 pKa = 10.75AGFKK130 pKa = 8.39EE131 pKa = 4.39TVMDD135 pKa = 3.99AEE137 pKa = 4.16EE138 pKa = 4.04NARR141 pKa = 11.84QKK143 pKa = 10.86LRR145 pKa = 11.84ANGLKK150 pKa = 10.42

Molecular weight: 16.67 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|P69548|J_BPAL3 DNA-binding protein J OS=Escherichia phage alpha3 OX=10849 GN=J PE=1 SV=1
MM1 pKa = 7.35KK2 pKa = 10.16KK3 pKa = 10.29ARR5 pKa = 11.84RR6 pKa = 11.84SPSRR10 pKa = 11.84RR11 pKa = 11.84KK12 pKa = 9.47GARR15 pKa = 11.84LWYY18 pKa = 10.44VGGSQFF24 pKa = 4.73

Molecular weight: 2.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10

0

10

1929

24

494

192.9

21.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.45 ± 1.15

1.192 ± 0.259

5.651 ± 0.524

4.873 ± 0.653

3.836 ± 0.613

6.065 ± 0.877

2.54 ± 0.502

4.821 ± 0.466

5.495 ± 1.008

8.657 ± 1.219

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.214 ± 0.315

4.769 ± 0.479

3.888 ± 0.732

5.391 ± 0.857

6.376 ± 0.684

8.087 ± 1.009

5.599 ± 0.389

6.221 ± 1.048

1.503 ± 0.255

3.37 ± 0.561
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski