Amino acid dipepetide frequency for Megasphaera sp. UPII 135-E

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
7.792AlaAla: 7.792 ± 0.213
1.214AlaCys: 1.214 ± 0.058
4.138AlaAsp: 4.138 ± 0.145
4.441AlaGlu: 4.441 ± 0.101
2.916AlaPhe: 2.916 ± 0.08
5.885AlaGly: 5.885 ± 0.139
1.627AlaHis: 1.627 ± 0.058
6.385AlaIle: 6.385 ± 0.123
5.177AlaLys: 5.177 ± 0.151
8.3AlaLeu: 8.3 ± 0.204
2.399AlaMet: 2.399 ± 0.085
2.855AlaAsn: 2.855 ± 0.106
2.322AlaPro: 2.322 ± 0.078
3.197AlaGln: 3.197 ± 0.143
3.321AlaArg: 3.321 ± 0.1
3.878AlaSer: 3.878 ± 0.09
4.398AlaThr: 4.398 ± 0.128
6.59AlaVal: 6.59 ± 0.142
0.761AlaTrp: 0.761 ± 0.041
3.07AlaTyr: 3.07 ± 0.074
0.0AlaXaa: 0.0 ± 0.0
Cys
0.878CysAla: 0.878 ± 0.054
0.232CysCys: 0.232 ± 0.025
0.649CysAsp: 0.649 ± 0.039
0.667CysGlu: 0.667 ± 0.043
0.496CysPhe: 0.496 ± 0.035
1.106CysGly: 1.106 ± 0.057
0.382CysHis: 0.382 ± 0.034
1.157CysIle: 1.157 ± 0.067
0.61CysLys: 0.61 ± 0.037
1.202CysLeu: 1.202 ± 0.058
0.429CysMet: 0.429 ± 0.032
0.378CysAsn: 0.378 ± 0.027
0.612CysPro: 0.612 ± 0.042
0.6CysGln: 0.6 ± 0.04
0.722CysArg: 0.722 ± 0.039
0.758CysSer: 0.758 ± 0.042
0.714CysThr: 0.714 ± 0.049
0.931CysVal: 0.931 ± 0.058
0.116CysTrp: 0.116 ± 0.015
0.474CysTyr: 0.474 ± 0.044
0.0CysXaa: 0.0 ± 0.0
Asp
3.886AspAla: 3.886 ± 0.096
0.606AspCys: 0.606 ± 0.037
2.576AspAsp: 2.576 ± 0.098
3.522AspGlu: 3.522 ± 0.103
2.082AspPhe: 2.082 ± 0.069
4.467AspGly: 4.467 ± 0.413
0.909AspHis: 0.909 ± 0.047
4.929AspIle: 4.929 ± 0.102
3.705AspLys: 3.705 ± 0.166
4.223AspLeu: 4.223 ± 0.096
1.739AspMet: 1.739 ± 0.061
2.16AspAsn: 2.16 ± 0.088
1.72AspPro: 1.72 ± 0.06
1.255AspGln: 1.255 ± 0.05
1.989AspArg: 1.989 ± 0.066
2.515AspSer: 2.515 ± 0.08
3.227AspThr: 3.227 ± 0.098
4.112AspVal: 4.112 ± 0.096
0.472AspTrp: 0.472 ± 0.033
2.003AspTyr: 2.003 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
5.088GluAla: 5.088 ± 0.123
0.58GluCys: 0.58 ± 0.036
3.024GluAsp: 3.024 ± 0.089
5.112GluGlu: 5.112 ± 0.149
1.887GluPhe: 1.887 ± 0.053
3.9GluGly: 3.9 ± 0.102
1.297GluHis: 1.297 ± 0.055
4.665GluIle: 4.665 ± 0.103
5.631GluLys: 5.631 ± 0.118
5.364GluLeu: 5.364 ± 0.116
2.111GluMet: 2.111 ± 0.067
2.841GluAsn: 2.841 ± 0.088
1.58GluPro: 1.58 ± 0.061
2.906GluGln: 2.906 ± 0.101
3.589GluArg: 3.589 ± 0.097
2.652GluSer: 2.652 ± 0.083
3.471GluThr: 3.471 ± 0.085
4.366GluVal: 4.366 ± 0.101
0.665GluTrp: 0.665 ± 0.049
2.139GluTyr: 2.139 ± 0.083
0.0GluXaa: 0.0 ± 0.0
Phe
2.912PheAla: 2.912 ± 0.083
0.638PheCys: 0.638 ± 0.036
2.042PheAsp: 2.042 ± 0.066
1.739PheGlu: 1.739 ± 0.071
1.787PhePhe: 1.787 ± 0.094
2.865PheGly: 2.865 ± 0.082
0.828PheHis: 0.828 ± 0.042
3.062PheIle: 3.062 ± 0.103
1.698PheLys: 1.698 ± 0.072
3.544PheLeu: 3.544 ± 0.129
1.049PheMet: 1.049 ± 0.05
1.356PheAsn: 1.356 ± 0.059
1.346PhePro: 1.346 ± 0.053
1.206PheGln: 1.206 ± 0.05
1.501PheArg: 1.501 ± 0.059
2.922PheSer: 2.922 ± 0.088
2.251PheThr: 2.251 ± 0.063
2.554PheVal: 2.554 ± 0.092
0.35PheTrp: 0.35 ± 0.027
1.279PheTyr: 1.279 ± 0.056
0.0PheXaa: 0.0 ± 0.0
Gly
5.616GlyAla: 5.616 ± 0.166
1.005GlyCys: 1.005 ± 0.049
3.676GlyAsp: 3.676 ± 0.114
4.122GlyGlu: 4.122 ± 0.104
2.82GlyPhe: 2.82 ± 0.069
5.01GlyGly: 5.01 ± 0.118
1.588GlyHis: 1.588 ± 0.066
6.422GlyIle: 6.422 ± 0.149
6.273GlyLys: 6.273 ± 0.306
5.95GlyLeu: 5.95 ± 0.137
2.519GlyMet: 2.519 ± 0.08
3.199GlyAsn: 3.199 ± 0.139
1.586GlyPro: 1.586 ± 0.059
2.469GlyGln: 2.469 ± 0.098
3.142GlyArg: 3.142 ± 0.083
3.977GlySer: 3.977 ± 0.122
4.97GlyThr: 4.97 ± 0.188
5.431GlyVal: 5.431 ± 0.12
0.655GlyTrp: 0.655 ± 0.039
2.863GlyTyr: 2.863 ± 0.093
0.0GlyXaa: 0.0 ± 0.0
His
1.655HisAla: 1.655 ± 0.058
0.352HisCys: 0.352 ± 0.029
1.104HisAsp: 1.104 ± 0.052
1.244HisGlu: 1.244 ± 0.053
0.887HisPhe: 0.887 ± 0.044
1.57HisGly: 1.57 ± 0.067
0.673HisHis: 0.673 ± 0.039
2.231HisIle: 2.231 ± 0.071
1.244HisLys: 1.244 ± 0.054
1.995HisLeu: 1.995 ± 0.071
0.655HisMet: 0.655 ± 0.037
0.87HisAsn: 0.87 ± 0.041
1.137HisPro: 1.137 ± 0.054
0.939HisGln: 0.939 ± 0.045
1.07HisArg: 1.07 ± 0.048
1.102HisSer: 1.102 ± 0.048
1.458HisThr: 1.458 ± 0.059
1.808HisVal: 1.808 ± 0.064
0.329HisTrp: 0.329 ± 0.028
0.842HisTyr: 0.842 ± 0.046
0.0HisXaa: 0.0 ± 0.0
Ile
6.432IleAla: 6.432 ± 0.136
1.204IleCys: 1.204 ± 0.064
3.977IleAsp: 3.977 ± 0.099
4.451IleGlu: 4.451 ± 0.105
2.786IlePhe: 2.786 ± 0.095
5.911IleGly: 5.911 ± 0.152
1.867IleHis: 1.867 ± 0.067
5.779IleIle: 5.779 ± 0.17
4.154IleLys: 4.154 ± 0.098
6.69IleLeu: 6.69 ± 0.174
1.966IleMet: 1.966 ± 0.075
2.778IleAsn: 2.778 ± 0.09
3.498IlePro: 3.498 ± 0.114
3.152IleGln: 3.152 ± 0.096
3.819IleArg: 3.819 ± 0.114
4.976IleSer: 4.976 ± 0.117
4.98IleThr: 4.98 ± 0.136
5.378IleVal: 5.378 ± 0.147
0.626IleTrp: 0.626 ± 0.038
2.532IleTyr: 2.532 ± 0.083
0.0IleXaa: 0.0 ± 0.0
Lys
5.273LysAla: 5.273 ± 0.173
0.413LysCys: 0.413 ± 0.033
4.051LysAsp: 4.051 ± 0.317
5.842LysGlu: 5.842 ± 0.131
1.46LysPhe: 1.46 ± 0.064
4.457LysGly: 4.457 ± 0.133
1.346LysHis: 1.346 ± 0.057
4.352LysIle: 4.352 ± 0.095
5.828LysLys: 5.828 ± 0.145
4.705LysLeu: 4.705 ± 0.107
2.06LysMet: 2.06 ± 0.063
3.359LysAsn: 3.359 ± 0.142
1.924LysPro: 1.924 ± 0.081
3.156LysGln: 3.156 ± 0.102
3.345LysArg: 3.345 ± 0.096
2.926LysSer: 2.926 ± 0.091
3.941LysThr: 3.941 ± 0.12
4.469LysVal: 4.469 ± 0.145
0.606LysTrp: 0.606 ± 0.039
2.294LysTyr: 2.294 ± 0.075
0.0LysXaa: 0.0 ± 0.0
Leu
7.92LeuAla: 7.92 ± 0.149
1.391LeuCys: 1.391 ± 0.064
4.474LeuAsp: 4.474 ± 0.088
5.084LeuGlu: 5.084 ± 0.106
3.601LeuPhe: 3.601 ± 0.122
6.845LeuGly: 6.845 ± 0.15
2.263LeuHis: 2.263 ± 0.083
5.633LeuIle: 5.633 ± 0.161
5.191LeuLys: 5.191 ± 0.111
8.492LeuLeu: 8.492 ± 0.227
2.233LeuMet: 2.233 ± 0.078
3.068LeuAsn: 3.068 ± 0.1
3.937LeuPro: 3.937 ± 0.113
4.809LeuGln: 4.809 ± 0.127
4.345LeuArg: 4.345 ± 0.1
5.773LeuSer: 5.773 ± 0.137
5.112LeuThr: 5.112 ± 0.11
6.031LeuVal: 6.031 ± 0.138
0.824LeuTrp: 0.824 ± 0.046
3.3LeuTyr: 3.3 ± 0.101
0.0LeuXaa: 0.0 ± 0.0
Met
2.623MetAla: 2.623 ± 0.083
0.289MetCys: 0.289 ± 0.024
1.59MetAsp: 1.59 ± 0.053
1.995MetGlu: 1.995 ± 0.077
0.763MetPhe: 0.763 ± 0.051
2.243MetGly: 2.243 ± 0.071
0.567MetHis: 0.567 ± 0.037
1.981MetIle: 1.981 ± 0.071
2.391MetLys: 2.391 ± 0.069
2.355MetLeu: 2.355 ± 0.084
0.761MetMet: 0.761 ± 0.041
1.48MetAsn: 1.48 ± 0.071
1.167MetPro: 1.167 ± 0.049
1.228MetGln: 1.228 ± 0.052
1.263MetArg: 1.263 ± 0.053
1.586MetSer: 1.586 ± 0.055
1.722MetThr: 1.722 ± 0.071
1.991MetVal: 1.991 ± 0.07
0.214MetTrp: 0.214 ± 0.022
0.901MetTyr: 0.901 ± 0.05
0.0MetXaa: 0.0 ± 0.0
Asn
2.853AsnAla: 2.853 ± 0.114
0.519AsnCys: 0.519 ± 0.032
1.968AsnAsp: 1.968 ± 0.1
2.369AsnGlu: 2.369 ± 0.079
1.198AsnPhe: 1.198 ± 0.048
2.993AsnGly: 2.993 ± 0.18
0.931AsnHis: 0.931 ± 0.047
3.331AsnIle: 3.331 ± 0.079
2.824AsnLys: 2.824 ± 0.125
3.026AsnLeu: 3.026 ± 0.099
1.295AsnMet: 1.295 ± 0.056
1.582AsnAsn: 1.582 ± 0.084
1.844AsnPro: 1.844 ± 0.062
1.366AsnGln: 1.366 ± 0.067
1.852AsnArg: 1.852 ± 0.062
1.958AsnSer: 1.958 ± 0.09
2.416AsnThr: 2.416 ± 0.105
2.951AsnVal: 2.951 ± 0.099
0.366AsnTrp: 0.366 ± 0.029
1.352AsnTyr: 1.352 ± 0.057
0.0AsnXaa: 0.0 ± 0.0
Pro
2.408ProAla: 2.408 ± 0.082
0.472ProCys: 0.472 ± 0.033
1.932ProAsp: 1.932 ± 0.068
2.57ProGlu: 2.57 ± 0.094
1.676ProPhe: 1.676 ± 0.061
2.223ProGly: 2.223 ± 0.071
1.019ProHis: 1.019 ± 0.049
2.664ProIle: 2.664 ± 0.095
1.932ProLys: 1.932 ± 0.072
3.666ProLeu: 3.666 ± 0.104
0.958ProMet: 0.958 ± 0.043
1.293ProAsn: 1.293 ± 0.051
0.978ProPro: 0.978 ± 0.051
1.46ProGln: 1.46 ± 0.061
1.263ProArg: 1.263 ± 0.054
1.918ProSer: 1.918 ± 0.064
2.2ProThr: 2.2 ± 0.091
2.904ProVal: 2.904 ± 0.09
0.362ProTrp: 0.362 ± 0.025
1.505ProTyr: 1.505 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
4.11GlnAla: 4.11 ± 0.132
0.484GlnCys: 0.484 ± 0.037
2.074GlnAsp: 2.074 ± 0.079
3.186GlnGlu: 3.186 ± 0.092
1.23GlnPhe: 1.23 ± 0.049
3.121GlnGly: 3.121 ± 0.099
1.088GlnHis: 1.088 ± 0.045
2.808GlnIle: 2.808 ± 0.091
2.808GlnLys: 2.808 ± 0.076
3.784GlnLeu: 3.784 ± 0.09
1.082GlnMet: 1.082 ± 0.046
1.265GlnAsn: 1.265 ± 0.062
1.135GlnPro: 1.135 ± 0.056
2.599GlnGln: 2.599 ± 0.115
2.326GlnArg: 2.326 ± 0.077
1.789GlnSer: 1.789 ± 0.066
1.993GlnThr: 1.993 ± 0.063
2.944GlnVal: 2.944 ± 0.094
0.504GlnTrp: 0.504 ± 0.037
1.684GlnTyr: 1.684 ± 0.063
0.0GlnXaa: 0.0 ± 0.0
Arg
3.04ArgAla: 3.04 ± 0.084
0.638ArgCys: 0.638 ± 0.034
2.277ArgAsp: 2.277 ± 0.069
3.186ArgGlu: 3.186 ± 0.096
1.869ArgPhe: 1.869 ± 0.071
2.824ArgGly: 2.824 ± 0.085
1.171ArgHis: 1.171 ± 0.048
3.827ArgIle: 3.827 ± 0.103
3.066ArgLys: 3.066 ± 0.077
4.659ArgLeu: 4.659 ± 0.114
1.531ArgMet: 1.531 ± 0.065
1.922ArgAsn: 1.922 ± 0.062
1.608ArgPro: 1.608 ± 0.065
2.43ArgGln: 2.43 ± 0.087
2.408ArgArg: 2.408 ± 0.073
2.29ArgSer: 2.29 ± 0.073
2.432ArgThr: 2.432 ± 0.074
3.068ArgVal: 3.068 ± 0.077
0.464ArgTrp: 0.464 ± 0.033
1.791ArgTyr: 1.791 ± 0.069
0.0ArgXaa: 0.0 ± 0.0
Ser
3.918SerAla: 3.918 ± 0.101
0.647SerCys: 0.647 ± 0.043
2.548SerAsp: 2.548 ± 0.07
2.812SerGlu: 2.812 ± 0.075
2.633SerPhe: 2.633 ± 0.092
4.11SerGly: 4.11 ± 0.089
1.385SerHis: 1.385 ± 0.062
4.449SerIle: 4.449 ± 0.105
3.02SerLys: 3.02 ± 0.104
5.698SerLeu: 5.698 ± 0.111
1.491SerMet: 1.491 ± 0.051
1.816SerAsn: 1.816 ± 0.065
1.938SerPro: 1.938 ± 0.071
1.952SerGln: 1.952 ± 0.076
2.448SerArg: 2.448 ± 0.072
3.081SerSer: 3.081 ± 0.087
3.073SerThr: 3.073 ± 0.106
4.073SerVal: 4.073 ± 0.109
0.533SerTrp: 0.533 ± 0.034
2.121SerTyr: 2.121 ± 0.069
0.0SerXaa: 0.0 ± 0.0
Thr
4.988ThrAla: 4.988 ± 0.125
0.74ThrCys: 0.74 ± 0.043
3.093ThrAsp: 3.093 ± 0.126
3.31ThrGlu: 3.31 ± 0.085
2.2ThrPhe: 2.2 ± 0.067
4.811ThrGly: 4.811 ± 0.126
1.24ThrHis: 1.24 ± 0.045
4.447ThrIle: 4.447 ± 0.119
3.343ThrLys: 3.343 ± 0.111
5.807ThrLeu: 5.807 ± 0.117
1.531ThrMet: 1.531 ± 0.068
2.32ThrAsn: 2.32 ± 0.126
2.664ThrPro: 2.664 ± 0.072
2.011ThrGln: 2.011 ± 0.077
2.243ThrArg: 2.243 ± 0.072
2.89ThrSer: 2.89 ± 0.084
3.54ThrThr: 3.54 ± 0.139
4.919ThrVal: 4.919 ± 0.18
0.602ThrTrp: 0.602 ± 0.037
2.404ThrTyr: 2.404 ± 0.08
0.0ThrXaa: 0.0 ± 0.0
Val
5.98ValAla: 5.98 ± 0.173
1.08ValCys: 1.08 ± 0.051
4.171ValAsp: 4.171 ± 0.117
4.358ValGlu: 4.358 ± 0.116
2.747ValPhe: 2.747 ± 0.09
5.342ValGly: 5.342 ± 0.135
1.608ValHis: 1.608 ± 0.056
5.336ValIle: 5.336 ± 0.114
4.065ValLys: 4.065 ± 0.139
6.879ValLeu: 6.879 ± 0.155
2.021ValMet: 2.021 ± 0.062
2.627ValAsn: 2.627 ± 0.102
2.755ValPro: 2.755 ± 0.092
3.085ValGln: 3.085 ± 0.094
3.39ValArg: 3.39 ± 0.095
4.335ValSer: 4.335 ± 0.094
4.539ValThr: 4.539 ± 0.161
5.708ValVal: 5.708 ± 0.117
0.626ValTrp: 0.626 ± 0.035
2.584ValTyr: 2.584 ± 0.079
0.0ValXaa: 0.0 ± 0.0
Trp
0.553TrpAla: 0.553 ± 0.036
0.11TrpCys: 0.11 ± 0.015
0.502TrpAsp: 0.502 ± 0.039
0.604TrpGlu: 0.604 ± 0.036
0.388TrpPhe: 0.388 ± 0.03
0.712TrpGly: 0.712 ± 0.044
0.333TrpHis: 0.333 ± 0.024
0.653TrpIle: 0.653 ± 0.036
0.673TrpLys: 0.673 ± 0.046
0.968TrpLeu: 0.968 ± 0.056
0.246TrpMet: 0.246 ± 0.021
0.447TrpAsn: 0.447 ± 0.032
0.272TrpPro: 0.272 ± 0.027
0.697TrpGln: 0.697 ± 0.046
0.51TrpArg: 0.51 ± 0.033
0.494TrpSer: 0.494 ± 0.034
0.368TrpThr: 0.368 ± 0.025
0.549TrpVal: 0.549 ± 0.037
0.14TrpTrp: 0.14 ± 0.017
0.411TrpTyr: 0.411 ± 0.046
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.741TyrAla: 2.741 ± 0.084
0.502TyrCys: 0.502 ± 0.036
2.231TyrAsp: 2.231 ± 0.072
2.253TyrGlu: 2.253 ± 0.082
1.503TyrPhe: 1.503 ± 0.061
2.898TyrGly: 2.898 ± 0.104
1.041TyrHis: 1.041 ± 0.051
2.847TyrIle: 2.847 ± 0.093
2.172TyrLys: 2.172 ± 0.079
3.018TyrLeu: 3.018 ± 0.099
1.031TyrMet: 1.031 ± 0.05
1.379TyrAsn: 1.379 ± 0.058
1.377TyrPro: 1.377 ± 0.059
1.499TyrGln: 1.499 ± 0.059
1.958TyrArg: 1.958 ± 0.06
1.924TyrSer: 1.924 ± 0.064
2.3TyrThr: 2.3 ± 0.082
2.458TyrVal: 2.458 ± 0.078
0.419TyrTrp: 0.419 ± 0.031
1.499TyrTyr: 1.499 ± 0.073
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1512 proteins (491780 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski