Halovirus HVTV-1
Average proteome isoelectric point is 4.94
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 173 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|L7TNT9|L7TNT9_9VIRU LAGLIDADG_2 domain-containing protein OS=Halovirus HVTV-1 OX=1262528 GN=143 PE=4 SV=1
MM1 pKa = 7.15 VKK3 pKa = 9.0 STFTFEE9 pKa = 3.6 EE10 pKa = 5.24 GEE12 pKa = 4.32 AIDD15 pKa = 3.83 VGGHH19 pKa = 5.62 GEE21 pKa = 3.8 HH22 pKa = 7.61 DD23 pKa = 3.94 YY24 pKa = 11.31 VFEE27 pKa = 4.72 SGEE30 pKa = 4.17 PVTDD34 pKa = 3.39 SGISSLVFEE43 pKa = 5.24 SGTGLGGGQLQYY55 pKa = 10.59 EE56 pKa = 4.51 INGGGLNTLEE66 pKa = 4.42 TIKK69 pKa = 9.89 TAEE72 pKa = 4.06 PHH74 pKa = 5.74 YY75 pKa = 10.67 KK76 pKa = 10.32 FYY78 pKa = 11.18 DD79 pKa = 4.21 ADD81 pKa = 3.77 LTDD84 pKa = 3.75 NNFDD88 pKa = 3.48 GSQTVEE94 pKa = 3.62 ADD96 pKa = 2.57 HH97 pKa = 7.04 GYY99 pKa = 10.41 FYY101 pKa = 10.77 DD102 pKa = 4.12 ARR104 pKa = 11.84 TLTVLAHH111 pKa = 6.74 EE112 pKa = 4.43 NTNTGSIAIIFTYY125 pKa = 10.64 AEE127 pKa = 4.09 PTTPIDD133 pKa = 3.5 EE134 pKa = 4.99 EE135 pKa = 4.4 YY136 pKa = 11.14 DD137 pKa = 3.66 NQVDD141 pKa = 4.46 FSTSVSVPTYY151 pKa = 9.4 AAVWDD156 pKa = 4.52 GEE158 pKa = 4.38 ANDD161 pKa = 4.1 EE162 pKa = 4.63 SIGDD166 pKa = 3.72 VFEE169 pKa = 4.11 STRR172 pKa = 11.84 AKK174 pKa = 10.59 NGVSIGTTDD183 pKa = 3.47 GFGLITDD190 pKa = 4.69 GFSDD194 pKa = 3.9 ITFTCNPDD202 pKa = 2.89 VSHH205 pKa = 7.16 PKK207 pKa = 10.54 VGDD210 pKa = 3.58 DD211 pKa = 3.51 QLEE214 pKa = 4.03 VDD216 pKa = 3.79 PEE218 pKa = 4.16 GWRR221 pKa = 11.84 LITEE225 pKa = 4.82 EE226 pKa = 4.99 GPPPDD231 pKa = 3.68 GGAYY235 pKa = 9.34 EE236 pKa = 4.85 WIDD239 pKa = 3.47 QSSSMAGSLRR249 pKa = 11.84 IVSSS253 pKa = 3.47
Molecular weight: 27.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.702
IPC2_protein 3.783
IPC_protein 3.783
Toseland 3.567
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.605
Grimsley 3.478
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.075
Thurlkill 3.617
EMBOSS 3.681
Sillero 3.897
Patrickios 1.189
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.786
Protein with the highest isoelectric point:
>tr|L7THS3|L7THS3_9VIRU Ribonucleoside-diphosphate reductase OS=Halovirus HVTV-1 OX=1262528 GN=21 PE=3 SV=1
MM1 pKa = 6.97 TPEE4 pKa = 4.51 EE5 pKa = 4.48 KK6 pKa = 9.93 RR7 pKa = 11.84 HH8 pKa = 4.13 MWLRR12 pKa = 11.84 LHH14 pKa = 6.84 WDD16 pKa = 3.55 VPPTTSRR23 pKa = 11.84 KK24 pKa = 8.67 KK25 pKa = 10.33 EE26 pKa = 3.46 FWEE29 pKa = 4.47 RR30 pKa = 11.84 YY31 pKa = 5.28 MRR33 pKa = 11.84 TRR35 pKa = 11.84 RR36 pKa = 3.46
Molecular weight: 4.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.301
IPC2_protein 9.736
IPC_protein 10.804
Toseland 11.023
ProMoST 11.477
Dawson 11.067
Bjellqvist 10.862
Wikipedia 11.359
Rodwell 11.155
Grimsley 11.111
Solomon 11.345
Lehninger 11.286
Nozaki 11.008
DTASelect 10.862
Thurlkill 11.008
EMBOSS 11.462
Sillero 11.023
Patrickios 10.95
IPC_peptide 11.345
IPC2_peptide 9.955
IPC2.peptide.svr19 9.014
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
173
0
173
32349
25
1313
187.0
21.12
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.801 ± 0.188
1.135 ± 0.113
9.935 ± 0.314
10.541 ± 0.339
3.125 ± 0.106
7.329 ± 0.219
2.3 ± 0.123
3.994 ± 0.12
3.988 ± 0.144
6.727 ± 0.172
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.501 ± 0.086
3.765 ± 0.115
3.898 ± 0.142
3.53 ± 0.125
6.022 ± 0.165
6.121 ± 0.17
5.722 ± 0.207
7.354 ± 0.15
1.799 ± 0.079
3.413 ± 0.104
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here