Amino acid dipepetide frequency for Clostridium sp. CAG:62

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.102AlaAla: 6.102 ± 0.105	0.997AlaCys: 0.997 ± 0.037	4.168AlaAsp: 4.168 ± 0.071	4.475AlaGlu: 4.475 ± 0.089	2.94AlaPhe: 2.94 ± 0.061	5.63AlaGly: 5.63 ± 0.108	1.011AlaHis: 1.011 ± 0.04	4.851AlaIle: 4.851 ± 0.088	6.436AlaLys: 6.436 ± 0.101	6.25AlaLeu: 6.25 ± 0.096	2.306AlaMet: 2.306 ± 0.057	2.946AlaAsn: 2.946 ± 0.066	1.949AlaPro: 1.949 ± 0.058	1.799AlaGln: 1.799 ± 0.047	2.413AlaArg: 2.413 ± 0.064	4.111AlaSer: 4.111 ± 0.073	3.473AlaThr: 3.473 ± 0.09	5.786AlaVal: 5.786 ± 0.092	0.585AlaTrp: 0.585 ± 0.028	2.752AlaTyr: 2.752 ± 0.062	0.0AlaXaa: 0.0 ± 0.0
Cys	0.946CysAla: 0.946 ± 0.036	0.256CysCys: 0.256 ± 0.02	0.868CysAsp: 0.868 ± 0.035	1.013CysGlu: 1.013 ± 0.039	0.71CysPhe: 0.71 ± 0.035	1.398CysGly: 1.398 ± 0.047	0.335CysHis: 0.335 ± 0.022	1.023CysIle: 1.023 ± 0.038	1.102CysLys: 1.102 ± 0.036	1.271CysLeu: 1.271 ± 0.043	0.484CysMet: 0.484 ± 0.027	0.582CysAsn: 0.582 ± 0.029	0.581CysPro: 0.581 ± 0.031	0.447CysGln: 0.447 ± 0.024	0.633CysArg: 0.633 ± 0.03	0.868CysSer: 0.868 ± 0.033	0.731CysThr: 0.731 ± 0.03	1.181CysVal: 1.181 ± 0.039	0.11CysTrp: 0.11 ± 0.011	0.627CysTyr: 0.627 ± 0.029	0.0CysXaa: 0.0 ± 0.0
Asp	4.116AspAla: 4.116 ± 0.078	0.838AspCys: 0.838 ± 0.034	3.185AspAsp: 3.185 ± 0.075	4.993AspGlu: 4.993 ± 0.084	2.637AspPhe: 2.637 ± 0.06	4.604AspGly: 4.604 ± 0.084	0.742AspHis: 0.742 ± 0.035	4.059AspIle: 4.059 ± 0.087	4.214AspLys: 4.214 ± 0.073	4.378AspLeu: 4.378 ± 0.085	1.815AspMet: 1.815 ± 0.049	2.25AspAsn: 2.25 ± 0.06	1.339AspPro: 1.339 ± 0.045	0.9AspGln: 0.9 ± 0.034	1.968AspArg: 1.968 ± 0.048	3.353AspSer: 3.353 ± 0.074	3.163AspThr: 3.163 ± 0.064	4.19AspVal: 4.19 ± 0.074	0.611AspTrp: 0.611 ± 0.031	2.874AspTyr: 2.874 ± 0.065	0.0AspXaa: 0.0 ± 0.0
Glu	4.753GluAla: 4.753 ± 0.081	0.857GluCys: 0.857 ± 0.029	4.194GluAsp: 4.194 ± 0.077	6.854GluGlu: 6.854 ± 0.123	2.474GluPhe: 2.474 ± 0.053	4.187GluGly: 4.187 ± 0.083	1.307GluHis: 1.307 ± 0.048	5.562GluIle: 5.562 ± 0.094	7.803GluLys: 7.803 ± 0.121	6.055GluLeu: 6.055 ± 0.105	2.4GluMet: 2.4 ± 0.053	4.307GluAsn: 4.307 ± 0.08	1.899GluPro: 1.899 ± 0.057	2.895GluGln: 2.895 ± 0.064	3.165GluArg: 3.165 ± 0.072	3.513GluSer: 3.513 ± 0.072	3.929GluThr: 3.929 ± 0.069	4.62GluVal: 4.62 ± 0.081	0.641GluTrp: 0.641 ± 0.028	3.041GluTyr: 3.041 ± 0.064	0.0GluXaa: 0.0 ± 0.0
Phe	3.01PheAla: 3.01 ± 0.07	0.873PheCys: 0.873 ± 0.037	2.591PheAsp: 2.591 ± 0.067	2.689PheGlu: 2.689 ± 0.064	1.952PhePhe: 1.952 ± 0.067	2.974PheGly: 2.974 ± 0.063	0.785PheHis: 0.785 ± 0.033	2.361PheIle: 2.361 ± 0.07	1.904PheLys: 1.904 ± 0.053	3.784PheLeu: 3.784 ± 0.086	1.163PheMet: 1.163 ± 0.041	1.384PheAsn: 1.384 ± 0.037	1.307PhePro: 1.307 ± 0.041	1.134PheGln: 1.134 ± 0.043	1.654PheArg: 1.654 ± 0.049	2.996PheSer: 2.996 ± 0.066	2.274PheThr: 2.274 ± 0.053	3.32PheVal: 3.32 ± 0.066	0.377PheTrp: 0.377 ± 0.02	1.902PheTyr: 1.902 ± 0.056	0.001PheXaa: 0.001 ± 0.001
Gly	4.699GlyAla: 4.699 ± 0.098	1.202GlyCys: 1.202 ± 0.048	3.453GlyAsp: 3.453 ± 0.07	4.878GlyGlu: 4.878 ± 0.087	2.84GlyPhe: 2.84 ± 0.063	4.575GlyGly: 4.575 ± 0.091	1.153GlyHis: 1.153 ± 0.04	5.71GlyIle: 5.71 ± 0.095	6.394GlyLys: 6.394 ± 0.114	5.185GlyLeu: 5.185 ± 0.087	2.363GlyMet: 2.363 ± 0.064	3.16GlyAsn: 3.16 ± 0.062	1.183GlyPro: 1.183 ± 0.038	1.995GlyGln: 1.995 ± 0.047	2.709GlyArg: 2.709 ± 0.058	3.951GlySer: 3.951 ± 0.072	4.212GlyThr: 4.212 ± 0.083	5.161GlyVal: 5.161 ± 0.083	0.601GlyTrp: 0.601 ± 0.03	3.173GlyTyr: 3.173 ± 0.062	0.0GlyXaa: 0.0 ± 0.0
His	1.099HisAla: 1.099 ± 0.04	0.349HisCys: 0.349 ± 0.021	0.878HisAsp: 0.878 ± 0.039	1.066HisGlu: 1.066 ± 0.035	0.835HisPhe: 0.835 ± 0.036	1.246HisGly: 1.246 ± 0.038	0.404HisHis: 0.404 ± 0.032	1.232HisIle: 1.232 ± 0.043	1.106HisLys: 1.106 ± 0.04	1.417HisLeu: 1.417 ± 0.042	0.537HisMet: 0.537 ± 0.024	0.72HisAsn: 0.72 ± 0.032	0.768HisPro: 0.768 ± 0.033	0.457HisGln: 0.457 ± 0.022	0.706HisArg: 0.706 ± 0.029	0.96HisSer: 0.96 ± 0.04	0.958HisThr: 0.958 ± 0.033	1.143HisVal: 1.143 ± 0.039	0.152HisTrp: 0.152 ± 0.015	0.818HisTyr: 0.818 ± 0.032	0.0HisXaa: 0.0 ± 0.0
Ile	5.365IleAla: 5.365 ± 0.093	1.323IleCys: 1.323 ± 0.045	3.877IleAsp: 3.877 ± 0.067	4.685IleGlu: 4.685 ± 0.084	2.715IlePhe: 2.715 ± 0.072	4.923IleGly: 4.923 ± 0.09	1.431IleHis: 1.431 ± 0.045	4.367IleIle: 4.367 ± 0.086	4.297IleLys: 4.297 ± 0.077	6.584IleLeu: 6.584 ± 0.126	1.778IleMet: 1.778 ± 0.05	2.682IleAsn: 2.682 ± 0.064	3.004IlePro: 3.004 ± 0.07	2.383IleGln: 2.383 ± 0.054	3.375IleArg: 3.375 ± 0.077	4.794IleSer: 4.794 ± 0.089	4.312IleThr: 4.312 ± 0.072	5.099IleVal: 5.099 ± 0.075	0.603IleTrp: 0.603 ± 0.031	2.851IleTyr: 2.851 ± 0.066	0.0IleXaa: 0.0 ± 0.0
Lys	5.382LysAla: 5.382 ± 0.093	0.868LysCys: 0.868 ± 0.034	4.519LysAsp: 4.519 ± 0.084	7.935LysGlu: 7.935 ± 0.117	2.033LysPhe: 2.033 ± 0.056	4.874LysGly: 4.874 ± 0.095	1.108LysHis: 1.108 ± 0.037	5.65LysIle: 5.65 ± 0.098	10.203LysLys: 10.203 ± 0.209	5.83LysLeu: 5.83 ± 0.093	2.662LysMet: 2.662 ± 0.063	4.568LysAsn: 4.568 ± 0.086	2.278LysPro: 2.278 ± 0.053	3.111LysGln: 3.111 ± 0.07	3.435LysArg: 3.435 ± 0.076	3.983LysSer: 3.983 ± 0.083	4.594LysThr: 4.594 ± 0.088	5.108LysVal: 5.108 ± 0.111	0.828LysTrp: 0.828 ± 0.035	3.178LysTyr: 3.178 ± 0.071	0.0LysXaa: 0.0 ± 0.0
Leu	6.398LeuAla: 6.398 ± 0.087	1.541LeuCys: 1.541 ± 0.048	4.63LeuAsp: 4.63 ± 0.085	5.572LeuGlu: 5.572 ± 0.093	3.956LeuPhe: 3.956 ± 0.09	5.257LeuGly: 5.257 ± 0.091	1.488LeuHis: 1.488 ± 0.045	5.402LeuIle: 5.402 ± 0.105	5.845LeuLys: 5.845 ± 0.093	8.08LeuLeu: 8.08 ± 0.139	2.434LeuMet: 2.434 ± 0.068	3.558LeuAsn: 3.558 ± 0.07	3.228LeuPro: 3.228 ± 0.067	2.879LeuGln: 2.879 ± 0.072	3.401LeuArg: 3.401 ± 0.072	6.277LeuSer: 6.277 ± 0.111	4.928LeuThr: 4.928 ± 0.09	5.913LeuVal: 5.913 ± 0.1	0.738LeuTrp: 0.738 ± 0.033	3.446LeuTyr: 3.446 ± 0.067	0.0LeuXaa: 0.0 ± 0.0
Met	2.45MetAla: 2.45 ± 0.059	0.357MetCys: 0.357 ± 0.024	1.935MetAsp: 1.935 ± 0.043	2.404MetGlu: 2.404 ± 0.062	0.996MetPhe: 0.996 ± 0.041	1.914MetGly: 1.914 ± 0.05	0.459MetHis: 0.459 ± 0.025	2.233MetIle: 2.233 ± 0.059	2.884MetLys: 2.884 ± 0.054	2.495MetLeu: 2.495 ± 0.055	0.953MetMet: 0.953 ± 0.042	1.522MetAsn: 1.522 ± 0.044	1.156MetPro: 1.156 ± 0.037	1.199MetGln: 1.199 ± 0.041	1.243MetArg: 1.243 ± 0.043	1.744MetSer: 1.744 ± 0.047	1.77MetThr: 1.77 ± 0.048	2.01MetVal: 2.01 ± 0.048	0.192MetTrp: 0.192 ± 0.014	0.967MetTyr: 0.967 ± 0.038	0.0MetXaa: 0.0 ± 0.0
Asn	3.07AsnAla: 3.07 ± 0.053	0.682AsnCys: 0.682 ± 0.027	2.223AsnAsp: 2.223 ± 0.061	3.177AsnGlu: 3.177 ± 0.057	1.607AsnPhe: 1.607 ± 0.046	3.521AsnGly: 3.521 ± 0.071	0.897AsnHis: 0.897 ± 0.037	3.082AsnIle: 3.082 ± 0.065	3.248AsnLys: 3.248 ± 0.065	3.761AsnLeu: 3.761 ± 0.064	1.401AsnMet: 1.401 ± 0.045	2.008AsnAsn: 2.008 ± 0.069	2.005AsnPro: 2.005 ± 0.056	1.78AsnGln: 1.78 ± 0.049	2.136AsnArg: 2.136 ± 0.05	2.379AsnSer: 2.379 ± 0.055	2.554AsnThr: 2.554 ± 0.059	3.024AsnVal: 3.024 ± 0.067	0.452AsnTrp: 0.452 ± 0.025	2.02AsnTyr: 2.02 ± 0.061	0.001AsnXaa: 0.001 ± 0.001
Pro	2.3ProAla: 2.3 ± 0.065	0.415ProCys: 0.415 ± 0.022	2.18ProAsp: 2.18 ± 0.056	2.866ProGlu: 2.866 ± 0.072	1.384ProPhe: 1.384 ± 0.041	2.082ProGly: 2.082 ± 0.056	0.484ProHis: 0.484 ± 0.024	2.148ProIle: 2.148 ± 0.047	2.223ProLys: 2.223 ± 0.056	2.476ProLeu: 2.476 ± 0.058	0.887ProMet: 0.887 ± 0.031	1.252ProAsn: 1.252 ± 0.042	0.612ProPro: 0.612 ± 0.026	0.975ProGln: 0.975 ± 0.037	0.871ProArg: 0.871 ± 0.035	1.937ProSer: 1.937 ± 0.05	1.874ProThr: 1.874 ± 0.076	2.704ProVal: 2.704 ± 0.068	0.3ProTrp: 0.3 ± 0.019	1.402ProTyr: 1.402 ± 0.052	0.0ProXaa: 0.0 ± 0.0
Gln	2.306GlnAla: 2.306 ± 0.058	0.419GlnCys: 0.419 ± 0.023	1.456GlnAsp: 1.456 ± 0.044	2.201GlnGlu: 2.201 ± 0.063	1.204GlnPhe: 1.204 ± 0.038	1.973GlnGly: 1.973 ± 0.055	0.395GlnHis: 0.395 ± 0.024	2.609GlnIle: 2.609 ± 0.061	3.302GlnLys: 3.302 ± 0.072	2.518GlnLeu: 2.518 ± 0.055	1.326GlnMet: 1.326 ± 0.045	1.703GlnAsn: 1.703 ± 0.045	0.821GlnPro: 0.821 ± 0.038	1.006GlnGln: 1.006 ± 0.04	1.181GlnArg: 1.181 ± 0.038	1.699GlnSer: 1.699 ± 0.042	1.907GlnThr: 1.907 ± 0.049	2.099GlnVal: 2.099 ± 0.055	0.301GlnTrp: 0.301 ± 0.018	1.359GlnTyr: 1.359 ± 0.047	0.0GlnXaa: 0.0 ± 0.0
Arg	2.445ArgAla: 2.445 ± 0.058	0.543ArgCys: 0.543 ± 0.029	2.042ArgAsp: 2.042 ± 0.054	3.39ArgGlu: 3.39 ± 0.068	1.722ArgPhe: 1.722 ± 0.043	2.369ArgGly: 2.369 ± 0.06	0.713ArgHis: 0.713 ± 0.032	3.193ArgIle: 3.193 ± 0.058	3.401ArgLys: 3.401 ± 0.073	3.372ArgLeu: 3.372 ± 0.077	1.497ArgMet: 1.497 ± 0.043	1.912ArgAsn: 1.912 ± 0.053	1.181ArgPro: 1.181 ± 0.038	1.494ArgGln: 1.494 ± 0.046	1.893ArgArg: 1.893 ± 0.056	1.897ArgSer: 1.897 ± 0.049	2.038ArgThr: 2.038 ± 0.048	2.639ArgVal: 2.639 ± 0.072	0.317ArgTrp: 0.317 ± 0.022	1.929ArgTyr: 1.929 ± 0.054	0.0ArgXaa: 0.0 ± 0.0
Ser	4.104SerAla: 4.104 ± 0.078	0.873SerCys: 0.873 ± 0.033	3.611SerAsp: 3.611 ± 0.064	4.111SerGlu: 4.111 ± 0.078	2.767SerPhe: 2.767 ± 0.062	4.85SerGly: 4.85 ± 0.091	1.112SerHis: 1.112 ± 0.032	4.026SerIle: 4.026 ± 0.074	4.194SerLys: 4.194 ± 0.071	5.415SerLeu: 5.415 ± 0.093	1.835SerMet: 1.835 ± 0.048	2.455SerAsn: 2.455 ± 0.068	1.574SerPro: 1.574 ± 0.043	1.828SerGln: 1.828 ± 0.056	2.27SerArg: 2.27 ± 0.05	3.876SerSer: 3.876 ± 0.095	2.99SerThr: 2.99 ± 0.067	4.687SerVal: 4.687 ± 0.09	0.489SerTrp: 0.489 ± 0.025	2.877SerTyr: 2.877 ± 0.066	0.0SerXaa: 0.0 ± 0.0
Thr	4.275ThrAla: 4.275 ± 0.106	0.688ThrCys: 0.688 ± 0.031	3.074ThrAsp: 3.074 ± 0.071	3.622ThrGlu: 3.622 ± 0.064	2.258ThrPhe: 2.258 ± 0.053	4.382ThrGly: 4.382 ± 0.088	0.858ThrHis: 0.858 ± 0.034	4.367ThrIle: 4.367 ± 0.083	4.498ThrLys: 4.498 ± 0.091	4.81ThrLeu: 4.81 ± 0.093	1.584ThrMet: 1.584 ± 0.048	2.43ThrAsn: 2.43 ± 0.063	2.348ThrPro: 2.348 ± 0.068	1.452ThrGln: 1.452 ± 0.049	1.729ThrArg: 1.729 ± 0.045	3.308ThrSer: 3.308 ± 0.087	3.128ThrThr: 3.128 ± 0.088	4.701ThrVal: 4.701 ± 0.1	0.504ThrTrp: 0.504 ± 0.03	2.439ThrTyr: 2.439 ± 0.06	0.0ThrXaa: 0.0 ± 0.0
Val	4.899ValAla: 4.899 ± 0.088	1.286ValCys: 1.286 ± 0.043	4.062ValAsp: 4.062 ± 0.068	4.598ValGlu: 4.598 ± 0.089	3.12ValPhe: 3.12 ± 0.076	4.161ValGly: 4.161 ± 0.074	1.031ValHis: 1.031 ± 0.041	5.201ValIle: 5.201 ± 0.077	5.776ValLys: 5.776 ± 0.11	6.722ValLeu: 6.722 ± 0.097	2.09ValMet: 2.09 ± 0.059	3.182ValAsn: 3.182 ± 0.071	2.58ValPro: 2.58 ± 0.051	1.917ValGln: 1.917 ± 0.047	2.774ValArg: 2.774 ± 0.066	5.131ValSer: 5.131 ± 0.087	4.929ValThr: 4.929 ± 0.124	5.452ValVal: 5.452 ± 0.091	0.598ValTrp: 0.598 ± 0.026	2.841ValTyr: 2.841 ± 0.058	0.0ValXaa: 0.0 ± 0.0
Trp	0.415TrpAla: 0.415 ± 0.025	0.126TrpCys: 0.126 ± 0.015	0.528TrpAsp: 0.528 ± 0.027	0.645TrpGlu: 0.645 ± 0.035	0.399TrpPhe: 0.399 ± 0.025	0.557TrpGly: 0.557 ± 0.028	0.202TrpHis: 0.202 ± 0.015	0.696TrpIle: 0.696 ± 0.03	0.786TrpLys: 0.786 ± 0.035	0.761TrpLeu: 0.761 ± 0.034	0.307TrpMet: 0.307 ± 0.02	0.548TrpAsn: 0.548 ± 0.028	0.172TrpPro: 0.172 ± 0.015	0.426TrpGln: 0.426 ± 0.025	0.361TrpArg: 0.361 ± 0.025	0.541TrpSer: 0.541 ± 0.029	0.42TrpThr: 0.42 ± 0.02	0.449TrpVal: 0.449 ± 0.026	0.092TrpTrp: 0.092 ± 0.011	0.42TrpTyr: 0.42 ± 0.021	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.845TyrAla: 2.845 ± 0.059	0.672TyrCys: 0.672 ± 0.033	2.756TyrAsp: 2.756 ± 0.065	3.28TyrGlu: 3.28 ± 0.073	1.889TyrPhe: 1.889 ± 0.057	3.122TyrGly: 3.122 ± 0.063	0.937TyrHis: 0.937 ± 0.035	2.674TyrIle: 2.674 ± 0.06	2.534TyrLys: 2.534 ± 0.06	3.809TyrLeu: 3.809 ± 0.079	1.059TyrMet: 1.059 ± 0.037	1.859TyrAsn: 1.859 ± 0.063	1.384TyrPro: 1.384 ± 0.044	1.707TyrGln: 1.707 ± 0.046	2.07TyrArg: 2.07 ± 0.054	2.626TyrSer: 2.626 ± 0.073	2.271TyrThr: 2.271 ± 0.064	3.064TyrVal: 3.064 ± 0.064	0.359TyrTrp: 0.359 ± 0.024	2.146TyrTyr: 2.146 ± 0.061	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.002XaaGlu: 0.002 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 2452 proteins (800385 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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