Amino acid dipepetide frequency for Rhizobium sp. AC44/96

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
15.648AlaAla: 15.648 ± 0.141
0.934AlaCys: 0.934 ± 0.023
6.786AlaAsp: 6.786 ± 0.071
7.005AlaGlu: 7.005 ± 0.073
4.578AlaPhe: 4.578 ± 0.054
9.767AlaGly: 9.767 ± 0.083
2.07AlaHis: 2.07 ± 0.034
7.165AlaIle: 7.165 ± 0.076
4.552AlaLys: 4.552 ± 0.057
12.262AlaLeu: 12.262 ± 0.106
3.413AlaMet: 3.413 ± 0.041
3.233AlaAsn: 3.233 ± 0.056
4.72AlaPro: 4.72 ± 0.068
3.503AlaGln: 3.503 ± 0.048
7.193AlaArg: 7.193 ± 0.083
6.881AlaSer: 6.881 ± 0.073
6.066AlaThr: 6.066 ± 0.06
8.674AlaVal: 8.674 ± 0.074
1.38AlaTrp: 1.38 ± 0.029
2.643AlaTyr: 2.643 ± 0.042
0.0AlaXaa: 0.0 ± 0.0
Cys
0.863CysAla: 0.863 ± 0.021
0.101CysCys: 0.101 ± 0.008
0.474CysAsp: 0.474 ± 0.018
0.4CysGlu: 0.4 ± 0.014
0.312CysPhe: 0.312 ± 0.014
0.894CysGly: 0.894 ± 0.024
0.241CysHis: 0.241 ± 0.012
0.409CysIle: 0.409 ± 0.018
0.203CysLys: 0.203 ± 0.011
0.791CysLeu: 0.791 ± 0.023
0.171CysMet: 0.171 ± 0.009
0.215CysAsn: 0.215 ± 0.012
0.36CysPro: 0.36 ± 0.014
0.226CysGln: 0.226 ± 0.011
0.518CysArg: 0.518 ± 0.018
0.468CysSer: 0.468 ± 0.019
0.39CysThr: 0.39 ± 0.015
0.603CysVal: 0.603 ± 0.016
0.097CysTrp: 0.097 ± 0.008
0.202CysTyr: 0.202 ± 0.011
0.0CysXaa: 0.0 ± 0.0
Asp
6.578AspAla: 6.578 ± 0.075
0.47AspCys: 0.47 ± 0.016
3.279AspAsp: 3.279 ± 0.072
3.473AspGlu: 3.473 ± 0.049
2.352AspPhe: 2.352 ± 0.041
5.147AspGly: 5.147 ± 0.072
1.266AspHis: 1.266 ± 0.033
3.487AspIle: 3.487 ± 0.044
2.058AspLys: 2.058 ± 0.038
5.627AspLeu: 5.627 ± 0.055
1.367AspMet: 1.367 ± 0.03
1.575AspAsn: 1.575 ± 0.033
3.109AspPro: 3.109 ± 0.047
1.778AspGln: 1.778 ± 0.033
3.994AspArg: 3.994 ± 0.052
2.309AspSer: 2.309 ± 0.042
2.673AspThr: 2.673 ± 0.059
4.397AspVal: 4.397 ± 0.06
0.925AspTrp: 0.925 ± 0.024
1.558AspTyr: 1.558 ± 0.028
0.0AspXaa: 0.0 ± 0.0
Glu
6.902GluAla: 6.902 ± 0.074
0.346GluCys: 0.346 ± 0.016
2.756GluAsp: 2.756 ± 0.041
3.352GluGlu: 3.352 ± 0.051
1.942GluPhe: 1.942 ± 0.034
3.979GluGly: 3.979 ± 0.042
1.203GluHis: 1.203 ± 0.024
3.797GluIle: 3.797 ± 0.051
2.676GluLys: 2.676 ± 0.043
5.316GluLeu: 5.316 ± 0.062
1.584GluMet: 1.584 ± 0.031
1.776GluAsn: 1.776 ± 0.031
2.519GluPro: 2.519 ± 0.051
2.046GluGln: 2.046 ± 0.038
4.517GluArg: 4.517 ± 0.062
2.378GluSer: 2.378 ± 0.037
3.545GluThr: 3.545 ± 0.045
3.617GluVal: 3.617 ± 0.053
0.7GluTrp: 0.7 ± 0.023
1.055GluTyr: 1.055 ± 0.027
0.0GluXaa: 0.0 ± 0.0
Phe
4.732PheAla: 4.732 ± 0.053
0.418PheCys: 0.418 ± 0.017
2.759PheAsp: 2.759 ± 0.041
2.178PheGlu: 2.178 ± 0.038
1.626PhePhe: 1.626 ± 0.035
3.78PheGly: 3.78 ± 0.049
0.804PheHis: 0.804 ± 0.026
1.945PheIle: 1.945 ± 0.035
1.27PheLys: 1.27 ± 0.028
3.591PheLeu: 3.591 ± 0.058
0.893PheMet: 0.893 ± 0.024
1.25PheAsn: 1.25 ± 0.029
1.619PhePro: 1.619 ± 0.033
1.126PheGln: 1.126 ± 0.025
2.2PheArg: 2.2 ± 0.037
2.619PheSer: 2.619 ± 0.04
2.015PheThr: 2.015 ± 0.037
3.114PheVal: 3.114 ± 0.047
0.567PheTrp: 0.567 ± 0.019
1.041PheTyr: 1.041 ± 0.026
0.0PheXaa: 0.0 ± 0.0
Gly
8.354GlyAla: 8.354 ± 0.079
0.797GlyCys: 0.797 ± 0.021
4.388GlyAsp: 4.388 ± 0.066
4.515GlyGlu: 4.515 ± 0.05
3.654GlyPhe: 3.654 ± 0.049
6.84GlyGly: 6.84 ± 0.106
1.83GlyHis: 1.83 ± 0.038
5.082GlyIle: 5.082 ± 0.052
3.848GlyLys: 3.848 ± 0.057
8.418GlyLeu: 8.418 ± 0.069
2.261GlyMet: 2.261 ± 0.035
2.504GlyAsn: 2.504 ± 0.054
3.022GlyPro: 3.022 ± 0.042
2.774GlyGln: 2.774 ± 0.039
5.326GlyArg: 5.326 ± 0.055
4.896GlySer: 4.896 ± 0.058
4.561GlyThr: 4.561 ± 0.072
5.778GlyVal: 5.778 ± 0.055
1.23GlyTrp: 1.23 ± 0.027
2.386GlyTyr: 2.386 ± 0.038
0.0GlyXaa: 0.0 ± 0.0
His
2.15HisAla: 2.15 ± 0.034
0.19HisCys: 0.19 ± 0.009
1.245HisAsp: 1.245 ± 0.028
1.096HisGlu: 1.096 ± 0.025
0.944HisPhe: 0.944 ± 0.022
1.836HisGly: 1.836 ± 0.033
0.565HisHis: 0.565 ± 0.025
1.031HisIle: 1.031 ± 0.026
0.547HisLys: 0.547 ± 0.018
2.021HisLeu: 2.021 ± 0.034
0.542HisMet: 0.542 ± 0.017
0.521HisAsn: 0.521 ± 0.016
1.209HisPro: 1.209 ± 0.031
0.579HisGln: 0.579 ± 0.019
1.317HisArg: 1.317 ± 0.028
1.066HisSer: 1.066 ± 0.025
0.816HisThr: 0.816 ± 0.024
1.511HisVal: 1.511 ± 0.028
0.304HisTrp: 0.304 ± 0.014
0.551HisTyr: 0.551 ± 0.019
0.0HisXaa: 0.0 ± 0.0
Ile
7.855IleAla: 7.855 ± 0.086
0.541IleCys: 0.541 ± 0.018
3.864IleAsp: 3.864 ± 0.049
3.69IleGlu: 3.69 ± 0.042
2.104IlePhe: 2.104 ± 0.038
5.379IleGly: 5.379 ± 0.065
0.973IleHis: 0.973 ± 0.023
2.857IleIle: 2.857 ± 0.049
1.805IleLys: 1.805 ± 0.033
5.023IleLeu: 5.023 ± 0.051
1.162IleMet: 1.162 ± 0.025
1.747IleAsn: 1.747 ± 0.036
2.493IlePro: 2.493 ± 0.04
1.322IleGln: 1.322 ± 0.031
3.454IleArg: 3.454 ± 0.052
3.669IleSer: 3.669 ± 0.048
2.939IleThr: 2.939 ± 0.045
4.805IleVal: 4.805 ± 0.056
0.673IleTrp: 0.673 ± 0.021
1.311IleTyr: 1.311 ± 0.031
0.0IleXaa: 0.0 ± 0.0
Lys
4.842LysAla: 4.842 ± 0.059
0.176LysCys: 0.176 ± 0.011
2.05LysAsp: 2.05 ± 0.039
1.922LysGlu: 1.922 ± 0.034
1.082LysPhe: 1.082 ± 0.026
2.863LysGly: 2.863 ± 0.044
0.704LysHis: 0.704 ± 0.018
2.191LysIle: 2.191 ± 0.037
1.607LysLys: 1.607 ± 0.036
3.778LysLeu: 3.778 ± 0.052
0.957LysMet: 0.957 ± 0.024
1.121LysAsn: 1.121 ± 0.026
2.307LysPro: 2.307 ± 0.042
1.262LysGln: 1.262 ± 0.028
2.569LysArg: 2.569 ± 0.042
2.311LysSer: 2.311 ± 0.038
2.401LysThr: 2.401 ± 0.041
2.733LysVal: 2.733 ± 0.044
0.433LysTrp: 0.433 ± 0.013
0.717LysTyr: 0.717 ± 0.02
0.0LysXaa: 0.0 ± 0.0
Leu
12.473LeuAla: 12.473 ± 0.096
0.887LeuCys: 0.887 ± 0.023
5.704LeuAsp: 5.704 ± 0.073
5.009LeuGlu: 5.009 ± 0.059
3.816LeuPhe: 3.816 ± 0.061
7.909LeuGly: 7.909 ± 0.076
1.797LeuHis: 1.797 ± 0.033
5.226LeuIle: 5.226 ± 0.065
4.135LeuLys: 4.135 ± 0.05
9.342LeuLeu: 9.342 ± 0.098
2.363LeuMet: 2.363 ± 0.039
2.817LeuAsn: 2.817 ± 0.045
5.125LeuPro: 5.125 ± 0.052
2.963LeuGln: 2.963 ± 0.045
5.97LeuArg: 5.97 ± 0.064
7.059LeuSer: 7.059 ± 0.074
5.644LeuThr: 5.644 ± 0.061
7.33LeuVal: 7.33 ± 0.065
1.077LeuTrp: 1.077 ± 0.025
2.13LeuTyr: 2.13 ± 0.034
0.0LeuXaa: 0.0 ± 0.0
Met
2.966MetAla: 2.966 ± 0.047
0.154MetCys: 0.154 ± 0.01
1.2MetAsp: 1.2 ± 0.028
1.222MetGlu: 1.222 ± 0.026
0.776MetPhe: 0.776 ± 0.021
1.724MetGly: 1.724 ± 0.032
0.432MetHis: 0.432 ± 0.018
1.543MetIle: 1.543 ± 0.033
1.132MetLys: 1.132 ± 0.027
2.513MetLeu: 2.513 ± 0.041
0.719MetMet: 0.719 ± 0.022
0.928MetAsn: 0.928 ± 0.024
1.48MetPro: 1.48 ± 0.03
0.865MetGln: 0.865 ± 0.024
1.749MetArg: 1.749 ± 0.034
1.842MetSer: 1.842 ± 0.032
1.984MetThr: 1.984 ± 0.03
1.726MetVal: 1.726 ± 0.033
0.191MetTrp: 0.191 ± 0.011
0.35MetTyr: 0.35 ± 0.014
0.0MetXaa: 0.0 ± 0.0
Asn
3.454AsnAla: 3.454 ± 0.05
0.238AsnCys: 0.238 ± 0.013
1.712AsnAsp: 1.712 ± 0.042
1.468AsnGlu: 1.468 ± 0.029
1.146AsnPhe: 1.146 ± 0.029
2.729AsnGly: 2.729 ± 0.057
0.573AsnHis: 0.573 ± 0.019
1.631AsnIle: 1.631 ± 0.032
0.853AsnLys: 0.853 ± 0.025
2.835AsnLeu: 2.835 ± 0.043
0.7AsnMet: 0.7 ± 0.021
0.876AsnAsn: 0.876 ± 0.026
1.902AsnPro: 1.902 ± 0.036
0.865AsnGln: 0.865 ± 0.02
1.918AsnArg: 1.918 ± 0.035
1.571AsnSer: 1.571 ± 0.034
1.439AsnThr: 1.439 ± 0.036
2.249AsnVal: 2.249 ± 0.04
0.481AsnTrp: 0.481 ± 0.017
0.749AsnTyr: 0.749 ± 0.021
0.0AsnXaa: 0.0 ± 0.0
Pro
5.461ProAla: 5.461 ± 0.066
0.256ProCys: 0.256 ± 0.012
3.289ProAsp: 3.289 ± 0.046
3.324ProGlu: 3.324 ± 0.047
1.948ProPhe: 1.948 ± 0.037
3.883ProGly: 3.883 ± 0.051
1.025ProHis: 1.025 ± 0.025
2.437ProIle: 2.437 ± 0.038
1.795ProLys: 1.795 ± 0.034
4.484ProLeu: 4.484 ± 0.049
1.104ProMet: 1.104 ± 0.029
1.398ProAsn: 1.398 ± 0.027
2.076ProPro: 2.076 ± 0.045
1.621ProGln: 1.621 ± 0.031
2.462ProArg: 2.462 ± 0.047
2.898ProSer: 2.898 ± 0.043
2.457ProThr: 2.457 ± 0.035
4.022ProVal: 4.022 ± 0.051
0.59ProTrp: 0.59 ± 0.017
1.224ProTyr: 1.224 ± 0.024
0.0ProXaa: 0.0 ± 0.0
Gln
3.849GlnAla: 3.849 ± 0.048
0.177GlnCys: 0.177 ± 0.01
1.492GlnAsp: 1.492 ± 0.031
1.514GlnGlu: 1.514 ± 0.033
1.118GlnPhe: 1.118 ± 0.03
2.191GlnGly: 2.191 ± 0.038
0.635GlnHis: 0.635 ± 0.017
1.957GlnIle: 1.957 ± 0.052
1.29GlnLys: 1.29 ± 0.029
3.009GlnLeu: 3.009 ± 0.046
0.946GlnMet: 0.946 ± 0.022
0.974GlnAsn: 0.974 ± 0.025
1.732GlnPro: 1.732 ± 0.039
1.374GlnGln: 1.374 ± 0.037
2.247GlnArg: 2.247 ± 0.036
1.99GlnSer: 1.99 ± 0.035
1.886GlnThr: 1.886 ± 0.035
2.115GlnVal: 2.115 ± 0.042
0.413GlnTrp: 0.413 ± 0.015
0.67GlnTyr: 0.67 ± 0.02
0.0GlnXaa: 0.0 ± 0.0
Arg
6.663ArgAla: 6.663 ± 0.071
0.425ArgCys: 0.425 ± 0.015
3.744ArgAsp: 3.744 ± 0.055
3.809ArgGlu: 3.809 ± 0.058
2.76ArgPhe: 2.76 ± 0.041
4.215ArgGly: 4.215 ± 0.055
1.522ArgHis: 1.522 ± 0.031
3.886ArgIle: 3.886 ± 0.05
2.485ArgLys: 2.485 ± 0.042
6.968ArgLeu: 6.968 ± 0.075
1.828ArgMet: 1.828 ± 0.032
1.96ArgAsn: 1.96 ± 0.029
3.013ArgPro: 3.013 ± 0.047
2.523ArgGln: 2.523 ± 0.045
4.765ArgArg: 4.765 ± 0.068
3.789ArgSer: 3.789 ± 0.051
3.07ArgThr: 3.07 ± 0.04
4.218ArgVal: 4.218 ± 0.048
0.866ArgTrp: 0.866 ± 0.024
1.706ArgTyr: 1.706 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.564SerAla: 6.564 ± 0.07
0.429SerCys: 0.429 ± 0.017
3.339SerAsp: 3.339 ± 0.045
3.108SerGlu: 3.108 ± 0.047
2.637SerPhe: 2.637 ± 0.04
5.91SerGly: 5.91 ± 0.072
1.194SerHis: 1.194 ± 0.026
3.501SerIle: 3.501 ± 0.042
2.074SerLys: 2.074 ± 0.035
5.969SerLeu: 5.969 ± 0.064
1.491SerMet: 1.491 ± 0.028
1.718SerAsn: 1.718 ± 0.031
2.75SerPro: 2.75 ± 0.046
1.873SerGln: 1.873 ± 0.044
3.75SerArg: 3.75 ± 0.054
3.698SerSer: 3.698 ± 0.064
3.05SerThr: 3.05 ± 0.045
4.465SerVal: 4.465 ± 0.055
0.801SerTrp: 0.801 ± 0.022
1.505SerTyr: 1.505 ± 0.033
0.0SerXaa: 0.0 ± 0.0
Thr
6.395ThrAla: 6.395 ± 0.074
0.395ThrCys: 0.395 ± 0.014
2.976ThrAsp: 2.976 ± 0.046
2.757ThrGlu: 2.757 ± 0.044
2.183ThrPhe: 2.183 ± 0.035
4.925ThrGly: 4.925 ± 0.06
1.031ThrHis: 1.031 ± 0.029
3.383ThrIle: 3.383 ± 0.051
1.793ThrLys: 1.793 ± 0.035
5.689ThrLeu: 5.689 ± 0.059
1.288ThrMet: 1.288 ± 0.025
1.509ThrAsn: 1.509 ± 0.039
2.974ThrPro: 2.974 ± 0.043
1.503ThrGln: 1.503 ± 0.031
3.01ThrArg: 3.01 ± 0.048
3.173ThrSer: 3.173 ± 0.054
3.1ThrThr: 3.1 ± 0.063
4.519ThrVal: 4.519 ± 0.057
0.632ThrTrp: 0.632 ± 0.02
1.303ThrTyr: 1.303 ± 0.031
0.0ThrXaa: 0.0 ± 0.0
Val
9.039ValAla: 9.039 ± 0.085
0.638ValCys: 0.638 ± 0.019
4.215ValAsp: 4.215 ± 0.055
4.329ValGlu: 4.329 ± 0.051
2.981ValPhe: 2.981 ± 0.047
5.54ValGly: 5.54 ± 0.053
1.366ValHis: 1.366 ± 0.032
4.352ValIle: 4.352 ± 0.052
2.611ValLys: 2.611 ± 0.044
7.263ValLeu: 7.263 ± 0.073
1.851ValMet: 1.851 ± 0.035
2.119ValAsn: 2.119 ± 0.036
3.561ValPro: 3.561 ± 0.05
2.02ValGln: 2.02 ± 0.036
4.556ValArg: 4.556 ± 0.053
4.989ValSer: 4.989 ± 0.048
4.57ValThr: 4.57 ± 0.06
6.032ValVal: 6.032 ± 0.061
0.847ValTrp: 0.847 ± 0.022
1.555ValTyr: 1.555 ± 0.031
0.0ValXaa: 0.0 ± 0.0
Trp
1.154TrpAla: 1.154 ± 0.024
0.134TrpCys: 0.134 ± 0.01
0.631TrpAsp: 0.631 ± 0.021
0.538TrpGlu: 0.538 ± 0.017
0.527TrpPhe: 0.527 ± 0.017
0.821TrpGly: 0.821 ± 0.026
0.309TrpHis: 0.309 ± 0.011
0.704TrpIle: 0.704 ± 0.021
0.543TrpLys: 0.543 ± 0.019
1.52TrpLeu: 1.52 ± 0.034
0.366TrpMet: 0.366 ± 0.013
0.479TrpAsn: 0.479 ± 0.018
0.649TrpPro: 0.649 ± 0.02
0.543TrpGln: 0.543 ± 0.017
0.996TrpArg: 0.996 ± 0.026
0.826TrpSer: 0.826 ± 0.023
0.755TrpThr: 0.755 ± 0.02
0.774TrpVal: 0.774 ± 0.02
0.212TrpTrp: 0.212 ± 0.012
0.305TrpTyr: 0.305 ± 0.014
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.534TyrAla: 2.534 ± 0.034
0.232TyrCys: 0.232 ± 0.012
1.517TyrAsp: 1.517 ± 0.032
1.296TyrGlu: 1.296 ± 0.026
1.062TyrPhe: 1.062 ± 0.021
2.132TyrGly: 2.132 ± 0.036
0.499TyrHis: 0.499 ± 0.017
1.08TyrIle: 1.08 ± 0.022
0.749TyrLys: 0.749 ± 0.02
2.359TyrLeu: 2.359 ± 0.044
0.49TyrMet: 0.49 ± 0.018
0.67TyrAsn: 0.67 ± 0.019
1.134TyrPro: 1.134 ± 0.027
0.811TyrGln: 0.811 ± 0.023
1.706TyrArg: 1.706 ± 0.032
1.393TyrSer: 1.393 ± 0.028
1.177TyrThr: 1.177 ± 0.031
1.752TyrVal: 1.752 ± 0.035
0.367TyrTrp: 0.367 ± 0.014
0.666TyrTyr: 0.666 ± 0.025
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5685 proteins (1779321 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski