Lynx rufus papillomavirus type 1
Average proteome isoelectric point is 6.34
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|I6LEI4|I6LEI4_9PAPI Replication protein E1 OS=Lynx rufus papillomavirus type 1 OX=323364 GN=E1 PE=3 SV=1
MM1 pKa = 7.47 IGSAPTISDD10 pKa = 3.01 IVLTEE15 pKa = 4.04 QPPEE19 pKa = 4.05 TADD22 pKa = 3.47 LRR24 pKa = 11.84 CYY26 pKa = 10.27 EE27 pKa = 3.95 QMPDD31 pKa = 3.42 EE32 pKa = 4.94 EE33 pKa = 4.61 EE34 pKa = 4.5 VEE36 pKa = 4.39 SQSRR40 pKa = 11.84 DD41 pKa = 3.4 LYY43 pKa = 10.75 RR44 pKa = 11.84 VSALCGLCGGGVRR57 pKa = 11.84 FACLSGSEE65 pKa = 4.4 DD66 pKa = 3.22 ILRR69 pKa = 11.84 LRR71 pKa = 11.84 SLLDD75 pKa = 3.81 TIQVVCVTCVKK86 pKa = 10.22 NQKK89 pKa = 9.66 LNHH92 pKa = 6.28 GGG94 pKa = 3.31
Molecular weight: 10.23 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.399
IPC2_protein 4.609
IPC_protein 4.457
Toseland 4.304
ProMoST 4.571
Dawson 4.406
Bjellqvist 4.558
Wikipedia 4.279
Rodwell 4.291
Grimsley 4.215
Solomon 4.393
Lehninger 4.355
Nozaki 4.52
DTASelect 4.66
Thurlkill 4.317
EMBOSS 4.291
Sillero 4.571
Patrickios 3.058
IPC_peptide 4.406
IPC2_peptide 4.558
IPC2.peptide.svr19 4.525
Protein with the highest isoelectric point:
>tr|I6LEI6|I6LEI6_9PAPI E4 protein (Fragment) OS=Lynx rufus papillomavirus type 1 OX=323364 GN=E4 PE=4 SV=1
MM1 pKa = 7.91 DD2 pKa = 5.94 NISKK6 pKa = 10.47 ALEE9 pKa = 4.17 SVQEE13 pKa = 4.05 QLLTLYY19 pKa = 10.47 EE20 pKa = 4.5 RR21 pKa = 11.84 DD22 pKa = 3.78 STDD25 pKa = 2.96 LTDD28 pKa = 5.84 QIKK31 pKa = 9.44 HH32 pKa = 4.09 WQLNRR37 pKa = 11.84 RR38 pKa = 11.84 EE39 pKa = 3.9 QALYY43 pKa = 10.0 YY44 pKa = 9.63 CARR47 pKa = 11.84 RR48 pKa = 11.84 QGITRR53 pKa = 11.84 VGMNVVPSLAASQQRR68 pKa = 11.84 AKK70 pKa = 10.78 SAIEE74 pKa = 3.94 QEE76 pKa = 4.57 LLLQSLLDD84 pKa = 3.56 SDD86 pKa = 5.31 FADD89 pKa = 4.58 EE90 pKa = 4.77 PWSLMDD96 pKa = 3.43 TSRR99 pKa = 11.84 EE100 pKa = 3.78 RR101 pKa = 11.84 LLADD105 pKa = 3.94 PPYY108 pKa = 9.84 CFKK111 pKa = 11.07 KK112 pKa = 10.61 GGQQVEE118 pKa = 4.01 LRR120 pKa = 11.84 YY121 pKa = 10.66 DD122 pKa = 3.51 RR123 pKa = 11.84 DD124 pKa = 3.34 RR125 pKa = 11.84 DD126 pKa = 3.53 NTSRR130 pKa = 11.84 HH131 pKa = 5.21 VLWKK135 pKa = 10.39 DD136 pKa = 2.87 IYY138 pKa = 10.73 FQGEE142 pKa = 3.66 EE143 pKa = 4.05 DD144 pKa = 3.44 TWRR147 pKa = 11.84 KK148 pKa = 6.47 THH150 pKa = 5.71 GQVDD154 pKa = 4.31 EE155 pKa = 4.99 KK156 pKa = 11.48 GLFYY160 pKa = 10.72 TDD162 pKa = 3.54 DD163 pKa = 4.19 KK164 pKa = 11.59 GVKK167 pKa = 7.79 TYY169 pKa = 10.64 YY170 pKa = 10.73 VEE172 pKa = 4.5 FEE174 pKa = 3.94 KK175 pKa = 10.95 EE176 pKa = 3.47 AAKK179 pKa = 10.4 FSKK182 pKa = 8.94 TGHH185 pKa = 5.82 YY186 pKa = 10.02 EE187 pKa = 3.99 VVSNLTTPVPTSTTTAAGLGHH208 pKa = 5.88 STSPGWATASGPPKK222 pKa = 10.29 KK223 pKa = 9.94 PSPAKK228 pKa = 9.69 KK229 pKa = 9.04 RR230 pKa = 11.84 KK231 pKa = 9.18 RR232 pKa = 11.84 PIRR235 pKa = 11.84 LSSPKK240 pKa = 9.8 TPRR243 pKa = 11.84 TGQRR247 pKa = 11.84 RR248 pKa = 11.84 RR249 pKa = 11.84 RR250 pKa = 11.84 QGEE253 pKa = 3.79 RR254 pKa = 11.84 AATSTPLPRR263 pKa = 11.84 PTPPSPEE270 pKa = 3.71 EE271 pKa = 3.88 VGRR274 pKa = 11.84 LTSSVPRR281 pKa = 11.84 QPGTRR286 pKa = 11.84 LGRR289 pKa = 11.84 LLHH292 pKa = 6.82 DD293 pKa = 4.2 ARR295 pKa = 11.84 DD296 pKa = 3.79 PPVLVLRR303 pKa = 11.84 GDD305 pKa = 3.88 ANSLKK310 pKa = 10.32 CIRR313 pKa = 11.84 YY314 pKa = 8.57 RR315 pKa = 11.84 LKK317 pKa = 10.93 GKK319 pKa = 10.63 YY320 pKa = 9.48 SDD322 pKa = 3.69 LFCRR326 pKa = 11.84 VSTTWTWTSPTGTDD340 pKa = 2.13 RR341 pKa = 11.84 WGRR344 pKa = 11.84 SRR346 pKa = 11.84 MLLTFRR352 pKa = 11.84 DD353 pKa = 3.81 LSQRR357 pKa = 11.84 DD358 pKa = 3.54 TFEE361 pKa = 3.83 RR362 pKa = 11.84 TVRR365 pKa = 11.84 LPKK368 pKa = 10.14 SVQAFRR374 pKa = 11.84 GSFEE378 pKa = 5.16 DD379 pKa = 4.07 YY380 pKa = 11.26
Molecular weight: 43.47 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.158
IPC2_protein 9.136
IPC_protein 9.18
Toseland 9.838
ProMoST 9.589
Dawson 10.072
Bjellqvist 9.765
Wikipedia 10.248
Rodwell 10.365
Grimsley 10.145
Solomon 10.101
Lehninger 10.072
Nozaki 9.882
DTASelect 9.75
Thurlkill 9.926
EMBOSS 10.262
Sillero 9.999
Patrickios 9.399
IPC_peptide 10.101
IPC2_peptide 8.434
IPC2.peptide.svr19 8.089
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
2337
94
608
333.9
37.54
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.905 ± 0.556
2.396 ± 0.887
6.29 ± 0.385
6.033 ± 0.367
4.108 ± 0.463
6.376 ± 0.521
1.797 ± 0.281
3.38 ± 0.706
4.792 ± 0.782
9.756 ± 0.796
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.54 ± 0.3
3.38 ± 0.826
7.06 ± 1.488
5.178 ± 0.228
7.274 ± 0.851
7.231 ± 0.859
6.333 ± 0.879
6.932 ± 0.907
1.369 ± 0.411
2.867 ± 0.392
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here