Jeotgalibaca sp. PTS2502
Average proteome isoelectric point is 5.93
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2116 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1U7EES4|A0A1U7EES4_9LACT Ion-translocating oxidoreductase complex subunit C OS=Jeotgalibaca sp. PTS2502 OX=1903686 GN=rnfC PE=3 SV=1
MM1 pKa = 7.46 LVLLLIFFMTLGSVGTVALANYY23 pKa = 8.37 TNVLVEE29 pKa = 3.88 ASVVNVRR36 pKa = 11.84 LGPGLSYY43 pKa = 11.05 DD44 pKa = 4.59 IMTQVQGGSSVNVLSEE60 pKa = 3.85 RR61 pKa = 11.84 NEE63 pKa = 4.02 WYY65 pKa = 10.1 KK66 pKa = 10.94 VRR68 pKa = 11.84 LDD70 pKa = 3.77 DD71 pKa = 6.27 GRR73 pKa = 11.84 IGWIASWLIDD83 pKa = 3.64 NTEE86 pKa = 3.91 VAASQNLMATVAASSVNVRR105 pKa = 11.84 EE106 pKa = 4.36 EE107 pKa = 4.15 NNSDD111 pKa = 3.58 SEE113 pKa = 4.67 IIGTASSGEE122 pKa = 4.1 QFNLLYY128 pKa = 10.57 EE129 pKa = 4.33 EE130 pKa = 5.07 NGWSQIAFNNRR141 pKa = 11.84 VGWILSEE148 pKa = 4.46 LVDD151 pKa = 3.62 ITPGTIEE158 pKa = 3.74 IEE160 pKa = 4.48 EE161 pKa = 4.52 IVTEE165 pKa = 4.55 TPSEE169 pKa = 4.41 TTDD172 pKa = 3.17 STVTITSEE180 pKa = 4.04 RR181 pKa = 11.84 VNLRR185 pKa = 11.84 IGPSLNDD192 pKa = 3.4 DD193 pKa = 3.84 VVYY196 pKa = 9.08 TASQGEE202 pKa = 4.63 SFTFSRR208 pKa = 11.84 SVGDD212 pKa = 3.63 FYY214 pKa = 11.37 EE215 pKa = 5.54 IILTDD220 pKa = 3.97 GSQAYY225 pKa = 8.17 VANWLVDD232 pKa = 3.87 LSGEE236 pKa = 4.22 SSQAEE241 pKa = 4.04 APVITTTLSEE251 pKa = 4.1 ATIVIDD257 pKa = 4.27 PGHH260 pKa = 6.57 GGSDD264 pKa = 3.33 PGALGSYY271 pKa = 9.96 VYY273 pKa = 10.46 EE274 pKa = 5.08 KK275 pKa = 10.9 DD276 pKa = 3.39 VTMSTAEE283 pKa = 3.77 QLAEE287 pKa = 4.0 KK288 pKa = 10.67 LEE290 pKa = 4.27 AVGANVILTRR300 pKa = 11.84 TTDD303 pKa = 3.09 ASVSLDD309 pKa = 3.25 EE310 pKa = 5.16 RR311 pKa = 11.84 VWISNSYY318 pKa = 10.19 RR319 pKa = 11.84 ADD321 pKa = 3.54 LFISLHH327 pKa = 5.86 YY328 pKa = 10.75 DD329 pKa = 3.19 STPEE333 pKa = 3.84 GMSASGFTTYY343 pKa = 10.93 YY344 pKa = 10.6 YY345 pKa = 11.06 DD346 pKa = 3.47 NTDD349 pKa = 3.0 SYY351 pKa = 11.76 LADD354 pKa = 3.69 LVNSKK359 pKa = 9.98 LAQNLPLPNNGVAFGDD375 pKa = 3.73 YY376 pKa = 10.73 LVLRR380 pKa = 11.84 EE381 pKa = 4.11 NTQPALLLEE390 pKa = 4.75 LGYY393 pKa = 10.41 MNNDD397 pKa = 2.24 SDD399 pKa = 5.54 AAVFDD404 pKa = 3.89 NNYY407 pKa = 9.95 YY408 pKa = 10.22 RR409 pKa = 11.84 DD410 pKa = 3.84 LVSTSILQALTEE422 pKa = 4.22 YY423 pKa = 10.55 FQQ425 pKa = 5.29
Molecular weight: 46.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.872
IPC_protein 3.859
Toseland 3.656
ProMoST 3.986
Dawson 3.821
Bjellqvist 3.973
Wikipedia 3.719
Rodwell 3.681
Grimsley 3.567
Solomon 3.821
Lehninger 3.783
Nozaki 3.935
DTASelect 4.126
Thurlkill 3.694
EMBOSS 3.745
Sillero 3.973
Patrickios 1.291
IPC_peptide 3.821
IPC2_peptide 3.948
IPC2.peptide.svr19 3.847
Protein with the highest isoelectric point:
>tr|A0A1U7EDA8|A0A1U7EDA8_9LACT Uncharacterized protein OS=Jeotgalibaca sp. PTS2502 OX=1903686 GN=BW721_08190 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.6 RR3 pKa = 11.84 TFQPKK8 pKa = 8.32 KK9 pKa = 9.49 RR10 pKa = 11.84 KK11 pKa = 8.83 RR12 pKa = 11.84 QTVHH16 pKa = 6.23 GFRR19 pKa = 11.84 KK20 pKa = 10.06 RR21 pKa = 11.84 MSTKK25 pKa = 10.02 NGRR28 pKa = 11.84 NVLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.28 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.516
IPC2_protein 11.213
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.735
Grimsley 13.013
Solomon 13.451
Lehninger 13.364
Nozaki 12.969
DTASelect 12.954
Thurlkill 12.969
EMBOSS 13.466
Sillero 12.969
Patrickios 12.457
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.137
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2116
0
2116
637522
29
2446
301.3
33.89
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.192 ± 0.059
0.559 ± 0.013
5.886 ± 0.045
7.519 ± 0.066
4.435 ± 0.045
6.364 ± 0.052
2.015 ± 0.025
7.744 ± 0.045
6.35 ± 0.051
9.739 ± 0.058
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.751 ± 0.024
4.57 ± 0.035
3.261 ± 0.028
4.119 ± 0.043
4.104 ± 0.039
6.173 ± 0.043
5.664 ± 0.034
6.903 ± 0.041
0.85 ± 0.017
3.804 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here