Methanonatronarchaeum thermophilum

Taxonomy: cellular organisms; Archaea; Euryarchaeota; Methanonatronarchaeia; Methanonatronarchaeales; Methanonatronarchaeaceae; Methanonatronarchaeum

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1510 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1Y3GAM7|A0A1Y3GAM7_9EURY Uncharacterized protein OS=Methanonatronarchaeum thermophilum OX=1927129 GN=AMET1_1228 PE=4 SV=1
MM1 pKa = 7.53VPRR4 pKa = 11.84KK5 pKa = 9.14YY6 pKa = 10.79LKK8 pKa = 10.81LLFVVCLLSSVLLVGGCVDD27 pKa = 3.44YY28 pKa = 11.32FEE30 pKa = 5.29SDD32 pKa = 3.06KK33 pKa = 11.28EE34 pKa = 4.1RR35 pKa = 11.84ITIVDD40 pKa = 3.55STGEE44 pKa = 4.17EE45 pKa = 3.84IEE47 pKa = 3.92INYY50 pKa = 9.21PVEE53 pKa = 4.08NVVTLTSDD61 pKa = 2.79SAEE64 pKa = 3.92AVRR67 pKa = 11.84ALGAEE72 pKa = 4.25DD73 pKa = 3.75KK74 pKa = 11.25VVGINDD80 pKa = 3.63YY81 pKa = 10.68MSGDD85 pKa = 3.19FWGDD89 pKa = 2.92LGEE92 pKa = 4.58IDD94 pKa = 4.17SVGNIFNPNPEE105 pKa = 4.64EE106 pKa = 4.07IASLEE111 pKa = 4.05PKK113 pKa = 9.74PDD115 pKa = 3.33LVLTYY120 pKa = 9.97TEE122 pKa = 4.16YY123 pKa = 11.14TEE125 pKa = 5.94DD126 pKa = 5.42LEE128 pKa = 7.12DD129 pKa = 4.0DD130 pKa = 4.66LKK132 pKa = 11.29PFGIDD137 pKa = 3.19VVRR140 pKa = 11.84LDD142 pKa = 4.04FYY144 pKa = 11.8KK145 pKa = 9.82MDD147 pKa = 4.74SIEE150 pKa = 4.15EE151 pKa = 4.22EE152 pKa = 4.06IEE154 pKa = 3.85ILGEE158 pKa = 3.9ILDD161 pKa = 4.53KK162 pKa = 11.1EE163 pKa = 4.4DD164 pKa = 3.7EE165 pKa = 4.4AEE167 pKa = 4.07EE168 pKa = 4.62LLNFYY173 pKa = 9.76NKK175 pKa = 10.26HH176 pKa = 4.77MDD178 pKa = 3.47EE179 pKa = 4.25VKK181 pKa = 10.69EE182 pKa = 3.7LAGDD186 pKa = 3.6INEE189 pKa = 4.5SEE191 pKa = 4.44VKK193 pKa = 10.23DD194 pKa = 3.74VYY196 pKa = 10.98IEE198 pKa = 4.57GFEE201 pKa = 4.12EE202 pKa = 4.49WSTASTNEE210 pKa = 4.08SNYY213 pKa = 8.79HH214 pKa = 4.55QVVEE218 pKa = 4.4LVGANNIAGDD228 pKa = 3.85RR229 pKa = 11.84DD230 pKa = 3.39RR231 pKa = 11.84TYY233 pKa = 9.86PTVSAEE239 pKa = 3.71WVLDD243 pKa = 3.94NNPDD247 pKa = 3.1AMMKK251 pKa = 9.82VVQDD255 pKa = 4.04SSVLGYY261 pKa = 10.51DD262 pKa = 3.71VNCTEE267 pKa = 5.28NAEE270 pKa = 4.04QMYY273 pKa = 10.91NDD275 pKa = 3.15IVDD278 pKa = 3.93RR279 pKa = 11.84EE280 pKa = 4.41GLSEE284 pKa = 3.97TDD286 pKa = 3.05AVKK289 pKa = 10.69NDD291 pKa = 3.35EE292 pKa = 4.58LVLVSQNVLSTMQNNIGTLIMAEE315 pKa = 3.93YY316 pKa = 9.86LYY318 pKa = 10.41PDD320 pKa = 3.87VYY322 pKa = 11.38EE323 pKa = 5.99DD324 pKa = 3.6IDD326 pKa = 3.56PMEE329 pKa = 3.86VHH331 pKa = 6.65EE332 pKa = 5.31EE333 pKa = 4.04YY334 pKa = 11.17LEE336 pKa = 4.52DD337 pKa = 3.64FHH339 pKa = 9.11GIEE342 pKa = 4.98YY343 pKa = 10.72DD344 pKa = 4.13GIWYY348 pKa = 9.64YY349 pKa = 11.67SKK351 pKa = 11.48

Molecular weight: 39.83 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1Y3GCC3|A0A1Y3GCC3_9EURY Uncharacterized protein OS=Methanonatronarchaeum thermophilum OX=1927129 GN=AMET1_0541 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 10.52SMYY5 pKa = 10.85SFIRR9 pKa = 11.84DD10 pKa = 2.9AWKK13 pKa = 10.3KK14 pKa = 9.11PRR16 pKa = 11.84EE17 pKa = 4.4GYY19 pKa = 8.44TRR21 pKa = 11.84EE22 pKa = 4.12LMWEE26 pKa = 4.27RR27 pKa = 11.84LQKK30 pKa = 9.42WRR32 pKa = 11.84SEE34 pKa = 4.11PTVKK38 pKa = 10.12RR39 pKa = 11.84VEE41 pKa = 3.99RR42 pKa = 11.84PTRR45 pKa = 11.84LDD47 pKa = 3.07KK48 pKa = 11.08ARR50 pKa = 11.84KK51 pKa = 8.7NGYY54 pKa = 8.07KK55 pKa = 10.16AKK57 pKa = 10.27EE58 pKa = 4.09GFVVARR64 pKa = 11.84ARR66 pKa = 11.84VRR68 pKa = 11.84RR69 pKa = 11.84GTRR72 pKa = 11.84RR73 pKa = 11.84KK74 pKa = 10.12SRR76 pKa = 11.84FKK78 pKa = 10.7KK79 pKa = 9.56GRR81 pKa = 11.84KK82 pKa = 6.64PSKK85 pKa = 9.8MGLEE89 pKa = 4.48KK90 pKa = 9.74ITPGKK95 pKa = 9.49SLQRR99 pKa = 11.84IAEE102 pKa = 4.06EE103 pKa = 3.76RR104 pKa = 11.84TSRR107 pKa = 11.84KK108 pKa = 9.25FPNLRR113 pKa = 11.84VLASYY118 pKa = 9.37WVGEE122 pKa = 4.43DD123 pKa = 5.13GRR125 pKa = 11.84QKK127 pKa = 8.93WYY129 pKa = 10.38EE130 pKa = 3.95VIMVDD135 pKa = 3.39THH137 pKa = 6.97HH138 pKa = 7.22PAIKK142 pKa = 10.2NDD144 pKa = 3.37SDD146 pKa = 4.16INWICSDD153 pKa = 2.97KK154 pKa = 11.04HH155 pKa = 6.16KK156 pKa = 11.13NRR158 pKa = 11.84AFRR161 pKa = 11.84GLTTVGKK168 pKa = 9.9DD169 pKa = 3.25GRR171 pKa = 11.84GLSEE175 pKa = 3.97RR176 pKa = 11.84GKK178 pKa = 9.09GTEE181 pKa = 3.71KK182 pKa = 9.85TRR184 pKa = 11.84PSIRR188 pKa = 11.84SNEE191 pKa = 4.24GKK193 pKa = 10.77GKK195 pKa = 10.49

Molecular weight: 22.91 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1510

0

1510

425233

31

1462

281.6

31.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.542 ± 0.064

0.987 ± 0.027

5.926 ± 0.065

8.586 ± 0.086

3.497 ± 0.051

7.076 ± 0.061

1.816 ± 0.03

8.741 ± 0.068

8.675 ± 0.1

8.696 ± 0.062

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.233 ± 0.028

4.904 ± 0.053

3.851 ± 0.039

3.203 ± 0.047

4.193 ± 0.051

5.451 ± 0.051

5.809 ± 0.06

6.694 ± 0.079

0.875 ± 0.023

3.245 ± 0.034
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski