Pseudomethylobacillus aquaticus

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Pseudomethylobacillus

Average proteome isoelectric point is 6.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2395 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3N0V1F3|A0A3N0V1F3_9PROT Cytokinin riboside 5'-monophosphate phosphoribohydrolase OS=Pseudomethylobacillus aquaticus OX=2676064 GN=ED236_07760 PE=3 SV=1
MM1 pKa = 7.63LANEE5 pKa = 4.61HH6 pKa = 5.04QQLWDD11 pKa = 3.66TPVVCRR17 pKa = 11.84VEE19 pKa = 3.93VDD21 pKa = 3.48LPGWLEE27 pKa = 3.91QLTGRR32 pKa = 11.84ADD34 pKa = 3.16WEE36 pKa = 4.25LFAEE40 pKa = 4.58EE41 pKa = 4.18EE42 pKa = 4.33DD43 pKa = 3.78EE44 pKa = 4.65AFVGFAMRR52 pKa = 11.84QGQQLAEE59 pKa = 3.61IRR61 pKa = 11.84LYY63 pKa = 10.25HH64 pKa = 6.28SGYY67 pKa = 11.09AVVDD71 pKa = 3.5VDD73 pKa = 5.18GEE75 pKa = 4.68SLFDD79 pKa = 3.82GSLTAGTSDD88 pKa = 3.35WAHH91 pKa = 6.31LSYY94 pKa = 10.97YY95 pKa = 9.89QADD98 pKa = 3.59SGEE101 pKa = 4.5PIVLNN106 pKa = 4.09

Molecular weight: 11.91 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3N0V2S9|A0A3N0V2S9_9PROT Uncharacterized protein OS=Pseudomethylobacillus aquaticus OX=2676064 GN=ED236_05210 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 8.87VRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.65GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2395

0

2395

762620

23

3492

318.4

34.93

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.148 ± 0.056

0.885 ± 0.019

5.414 ± 0.041

5.368 ± 0.04

3.51 ± 0.03

7.529 ± 0.058

2.432 ± 0.033

5.24 ± 0.035

3.878 ± 0.047

11.13 ± 0.081

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.58 ± 0.027

3.255 ± 0.044

4.602 ± 0.037

4.873 ± 0.043

5.946 ± 0.049

5.929 ± 0.036

5.146 ± 0.051

7.155 ± 0.048

1.289 ± 0.02

2.69 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski