Streptococcus phage P738
Average proteome isoelectric point is 5.88
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 48 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A516KPW7|A0A516KPW7_9CAUD Putative host-specificity tail protein OS=Streptococcus phage P738 OX=1814015 PE=4 SV=1
MM1 pKa = 7.29 EE2 pKa = 4.26 VNRR5 pKa = 11.84 RR6 pKa = 11.84 LEE8 pKa = 3.75 QLRR11 pKa = 11.84 QEE13 pKa = 4.67 WLNQPNLTDD22 pKa = 3.52 TLKK25 pKa = 10.81 EE26 pKa = 3.79 YY27 pKa = 10.9 LNEE30 pKa = 3.97 YY31 pKa = 10.37 FYY33 pKa = 11.39 DD34 pKa = 4.51 DD35 pKa = 3.52 YY36 pKa = 11.62 SYY38 pKa = 11.58 CEE40 pKa = 3.89 QCDD43 pKa = 4.07 KK44 pKa = 10.86 IASDD48 pKa = 5.35 SDD50 pKa = 3.49 WFWYY54 pKa = 10.1 EE55 pKa = 3.89 GEE57 pKa = 5.19 DD58 pKa = 3.63 YY59 pKa = 10.98 TDD61 pKa = 4.07 YY62 pKa = 11.83 LHH64 pKa = 7.56 IDD66 pKa = 3.51 CNEE69 pKa = 3.81 DD70 pKa = 3.21 KK71 pKa = 10.69 FYY73 pKa = 10.79 EE74 pKa = 4.54 TIKK77 pKa = 10.88 KK78 pKa = 9.23 PLEE81 pKa = 4.45 GGDD84 pKa = 3.49 QRR86 pKa = 11.84 KK87 pKa = 6.53 TT88 pKa = 3.29
Molecular weight: 10.9 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.931
IPC2_protein 4.215
IPC_protein 4.139
Toseland 3.961
ProMoST 4.279
Dawson 4.113
Bjellqvist 4.266
Wikipedia 4.012
Rodwell 3.973
Grimsley 3.872
Solomon 4.101
Lehninger 4.05
Nozaki 4.228
DTASelect 4.406
Thurlkill 3.986
EMBOSS 4.024
Sillero 4.253
Patrickios 1.074
IPC_peptide 4.101
IPC2_peptide 4.24
IPC2.peptide.svr19 4.151
Protein with the highest isoelectric point:
>tr|A0A516KPV9|A0A516KPV9_9CAUD Uncharacterized protein OS=Streptococcus phage P738 OX=1814015 PE=4 SV=1
MM1 pKa = 6.79 STSGFEE7 pKa = 4.26 SFLNKK12 pKa = 10.26 LKK14 pKa = 9.61 NTDD17 pKa = 3.63 IIKK20 pKa = 10.6 SGVDD24 pKa = 3.04 KK25 pKa = 11.17 AVADD29 pKa = 3.98 SLTRR33 pKa = 11.84 MYY35 pKa = 11.16 NRR37 pKa = 11.84 ASKK40 pKa = 10.53 RR41 pKa = 11.84 PYY43 pKa = 9.39 TPIDD47 pKa = 3.12 TGEE50 pKa = 4.28 LKK52 pKa = 10.73 NSRR55 pKa = 11.84 TKK57 pKa = 10.37 TRR59 pKa = 11.84 VSNGSGEE66 pKa = 3.87 FGYY69 pKa = 10.8 NKK71 pKa = 9.83 EE72 pKa = 3.71 YY73 pKa = 10.7 ARR75 pKa = 11.84 RR76 pKa = 11.84 VEE78 pKa = 3.82 YY79 pKa = 10.55 GGRR82 pKa = 11.84 DD83 pKa = 2.87 RR84 pKa = 11.84 KK85 pKa = 10.4 GRR87 pKa = 11.84 YY88 pKa = 7.49 IAPRR92 pKa = 11.84 RR93 pKa = 11.84 YY94 pKa = 8.67 LQRR97 pKa = 11.84 NVGVEE102 pKa = 3.62 KK103 pKa = 10.15 TLFQKK108 pKa = 10.39 DD109 pKa = 3.54 VKK111 pKa = 10.66 KK112 pKa = 10.79 ALEE115 pKa = 4.18 DD116 pKa = 3.35
Molecular weight: 13.29 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.191
IPC2_protein 9.545
IPC_protein 9.706
Toseland 10.262
ProMoST 9.897
Dawson 10.423
Bjellqvist 10.072
Wikipedia 10.584
Rodwell 10.877
Grimsley 10.482
Solomon 10.452
Lehninger 10.423
Nozaki 10.218
DTASelect 10.072
Thurlkill 10.277
EMBOSS 10.643
Sillero 10.335
Patrickios 10.555
IPC_peptide 10.452
IPC2_peptide 8.609
IPC2.peptide.svr19 8.682
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
48
0
48
10501
41
1323
218.8
24.82
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.228 ± 0.507
0.438 ± 0.075
5.961 ± 0.237
8.18 ± 0.61
4.495 ± 0.178
6.628 ± 0.434
1.257 ± 0.166
6.733 ± 0.37
8.333 ± 0.604
7.275 ± 0.228
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.314 ± 0.205
6.695 ± 0.348
2.409 ± 0.197
3.295 ± 0.166
3.857 ± 0.231
6.457 ± 0.448
6.637 ± 0.443
6.771 ± 0.242
1.2 ± 0.099
4.838 ± 0.333
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here