Pseudomonas phage PP7 (Bacteriophage PP7)

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Leviviricetes; Norzivirales; Fiersviridae; Pepevirus; Pepevirus rubrum

Average proteome isoelectric point is 8.31

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q38063|Q38063_BPPP7 Lysis protein OS=Pseudomonas phage PP7 OX=12023 PE=4 SV=1
MM1 pKa = 7.63SKK3 pKa = 9.74TIVLSVGEE11 pKa = 4.08ATRR14 pKa = 11.84TLTEE18 pKa = 3.88IQSTADD24 pKa = 3.48RR25 pKa = 11.84QIFEE29 pKa = 4.61EE30 pKa = 4.55KK31 pKa = 10.4VGPLVGRR38 pKa = 11.84LRR40 pKa = 11.84LTASLRR46 pKa = 11.84QNGAKK51 pKa = 8.67TAYY54 pKa = 9.7RR55 pKa = 11.84VNLKK59 pKa = 10.11LDD61 pKa = 3.21QADD64 pKa = 3.78VVDD67 pKa = 5.53CSTSVCGEE75 pKa = 4.12LPKK78 pKa = 10.45VRR80 pKa = 11.84YY81 pKa = 6.21TQVWSHH87 pKa = 6.9DD88 pKa = 3.53VTIVANSTEE97 pKa = 4.06ASRR100 pKa = 11.84KK101 pKa = 9.33SLYY104 pKa = 10.76DD105 pKa = 3.29LTKK108 pKa = 10.78SLVATSQVEE117 pKa = 4.12DD118 pKa = 3.68LVVNLVPLGRR128 pKa = 4.78

Molecular weight: 14.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q38064|Q38064_BPPP7 RNA replicase beta chain OS=Pseudomonas phage PP7 OX=12023 PE=4 SV=1
MM1 pKa = 7.9SSTLCRR7 pKa = 11.84WAVKK11 pKa = 10.16ALRR14 pKa = 11.84CTRR17 pKa = 11.84VYY19 pKa = 10.99KK20 pKa = 10.34EE21 pKa = 4.89FIWKK25 pKa = 9.42PLVALSYY32 pKa = 9.16VTLYY36 pKa = 11.0LLSSVFLSQLSYY48 pKa = 10.81PIGSWAVV55 pKa = 3.05

Molecular weight: 6.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4

0

4

1184

55

552

296.0

33.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.588 ± 0.484

1.605 ± 0.165

5.152 ± 0.472

6.166 ± 0.528

4.307 ± 0.595

6.25 ± 0.727

1.943 ± 0.336

3.885 ± 0.349

4.645 ± 0.178

11.149 ± 0.584

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.436 ± 0.123

2.365 ± 0.179

5.152 ± 0.693

2.449 ± 0.391

8.953 ± 0.52

8.361 ± 0.623

5.574 ± 1.021

8.024 ± 0.993

1.943 ± 0.25

3.97 ± 0.44
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski