Pustulibacterium marinum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Pustulibacterium

Average proteome isoelectric point is 6.27

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3719 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1I7FBT0|A0A1I7FBT0_9FLAO ZIP Zinc transporter OS=Pustulibacterium marinum OX=1224947 GN=SAMN05216480_101826 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.44KK3 pKa = 8.49ITFLFLLVASFTLNAQLVVNEE24 pKa = 4.41VDD26 pKa = 3.34ADD28 pKa = 4.09TPGVDD33 pKa = 3.01NQEE36 pKa = 4.32FIEE39 pKa = 4.67LKK41 pKa = 10.49SDD43 pKa = 3.73TPNLSLDD50 pKa = 3.69GYY52 pKa = 10.47VVVLFNGNGDD62 pKa = 3.47YY63 pKa = 10.8SYY65 pKa = 11.75YY66 pKa = 11.0SVDD69 pKa = 3.54LDD71 pKa = 5.28GYY73 pKa = 7.38TTDD76 pKa = 4.01DD77 pKa = 3.41NGIIVIGSAGVSPFPGILISANVIQNGADD106 pKa = 2.96AVAIYY111 pKa = 9.99QGSWYY116 pKa = 9.58DD117 pKa = 3.8YY118 pKa = 11.24PEE120 pKa = 4.55DD121 pKa = 4.01TPPTTEE127 pKa = 3.89NLIDD131 pKa = 3.85ALVYY135 pKa = 8.54DD136 pKa = 4.92TNDD139 pKa = 3.25SDD141 pKa = 4.91DD142 pKa = 3.98TGLMAALGVTTQTNEE157 pKa = 3.78GGNGSTTSNSVQRR170 pKa = 11.84MNDD173 pKa = 3.06GTYY176 pKa = 10.91VSATPTPGLPNDD188 pKa = 4.04GSGVVYY194 pKa = 10.91NGITAQLSANEE205 pKa = 4.07FVEE208 pKa = 4.46GDD210 pKa = 3.7SFSITFTTEE219 pKa = 3.73DD220 pKa = 3.8NVDD223 pKa = 3.32EE224 pKa = 4.43TLVFDD229 pKa = 4.97ISFDD233 pKa = 3.68NEE235 pKa = 4.14GFSSEE240 pKa = 4.82DD241 pKa = 2.94ISGNFSISIASGTSSVTEE259 pKa = 4.42TYY261 pKa = 10.63QIIDD265 pKa = 3.67DD266 pKa = 4.37TEE268 pKa = 5.33DD269 pKa = 3.19EE270 pKa = 4.26GDD272 pKa = 3.68EE273 pKa = 4.34VFVLNMINIPAGFNLLNNLLEE294 pKa = 4.25GTITDD299 pKa = 4.24DD300 pKa = 5.63DD301 pKa = 5.16YY302 pKa = 11.91STSAWGTPLNPTYY315 pKa = 10.91GIVSSTAPDD324 pKa = 3.32NYY326 pKa = 10.49YY327 pKa = 11.12SSLDD331 pKa = 3.81GLSGAALEE339 pKa = 4.78QAIQDD344 pKa = 4.34IIADD348 pKa = 4.03PNVVRR353 pKa = 11.84AQNYY357 pKa = 10.39GDD359 pKa = 3.41VTDD362 pKa = 4.63ILKK365 pKa = 10.69QSDD368 pKa = 3.72QNPEE372 pKa = 3.8NNNEE376 pKa = 3.21VWLIYY381 pKa = 9.81TEE383 pKa = 4.09QPRR386 pKa = 11.84AKK388 pKa = 9.47IDD390 pKa = 3.6FQTTSNNTGTWNRR403 pKa = 11.84EE404 pKa = 3.61HH405 pKa = 7.71IYY407 pKa = 9.15PQSRR411 pKa = 11.84GGFSGGTEE419 pKa = 4.05YY420 pKa = 10.75EE421 pKa = 3.67ADD423 pKa = 4.67GIDD426 pKa = 4.16VYY428 pKa = 11.55LPTNADD434 pKa = 4.25DD435 pKa = 4.03ILAGHH440 pKa = 7.38ADD442 pKa = 3.26AHH444 pKa = 6.7HH445 pKa = 6.54IRR447 pKa = 11.84AVDD450 pKa = 3.84GPEE453 pKa = 3.56NSSRR457 pKa = 11.84GNQDD461 pKa = 3.17YY462 pKa = 11.25GSGAYY467 pKa = 8.64EE468 pKa = 4.43GPTANAGSWKK478 pKa = 10.43GDD480 pKa = 3.25VARR483 pKa = 11.84SLFYY487 pKa = 10.25MAIRR491 pKa = 11.84YY492 pKa = 8.7NDD494 pKa = 3.7LDD496 pKa = 4.01VVNGNPNDD504 pKa = 3.78STVGQLGDD512 pKa = 3.78LATLLVWNTQDD523 pKa = 3.64PADD526 pKa = 4.13DD527 pKa = 4.26FEE529 pKa = 4.99MNRR532 pKa = 11.84NNYY535 pKa = 9.06IYY537 pKa = 9.19TWQYY541 pKa = 9.7NRR543 pKa = 11.84NPFIDD548 pKa = 4.03YY549 pKa = 10.39PEE551 pKa = 4.22LADD554 pKa = 5.65YY555 pKa = 10.68IWGDD559 pKa = 3.33NAGEE563 pKa = 4.24VWNLPMSTHH572 pKa = 5.68QEE574 pKa = 3.28ILEE577 pKa = 4.11AVKK580 pKa = 10.17IYY582 pKa = 9.57PNPINSYY589 pKa = 8.94FQITGISEE597 pKa = 4.07EE598 pKa = 4.14FKK600 pKa = 10.02ITIMDD605 pKa = 3.66STGKK609 pKa = 10.17VVLSKK614 pKa = 11.11SDD616 pKa = 3.08VDD618 pKa = 4.36SNRR621 pKa = 11.84KK622 pKa = 9.29IYY624 pKa = 10.18IDD626 pKa = 4.04GSAGIYY632 pKa = 10.2LVTIEE637 pKa = 4.53TEE639 pKa = 3.46KK640 pKa = 10.37GTIRR644 pKa = 11.84KK645 pKa = 9.45KK646 pKa = 9.76IIKK649 pKa = 8.5MM650 pKa = 3.59

Molecular weight: 70.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1I7G5E1|A0A1I7G5E1_9FLAO OstA-like protein OS=Pustulibacterium marinum OX=1224947 GN=SAMN05216480_103196 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.59RR3 pKa = 11.84TFQPSKK9 pKa = 9.13RR10 pKa = 11.84KK11 pKa = 9.48RR12 pKa = 11.84RR13 pKa = 11.84NKK15 pKa = 9.49HH16 pKa = 3.94GFRR19 pKa = 11.84EE20 pKa = 4.27RR21 pKa = 11.84MASANGRR28 pKa = 11.84KK29 pKa = 9.12VLASRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.05GRR39 pKa = 11.84KK40 pKa = 8.34KK41 pKa = 10.52LSVSSEE47 pKa = 3.87PRR49 pKa = 11.84HH50 pKa = 5.92KK51 pKa = 10.61KK52 pKa = 9.84

Molecular weight: 6.16 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3719

0

3719

1275365

29

2940

342.9

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.676 ± 0.038

0.773 ± 0.014

5.615 ± 0.037

6.898 ± 0.037

5.163 ± 0.034

6.303 ± 0.038

1.85 ± 0.018

7.422 ± 0.041

7.323 ± 0.05

9.032 ± 0.043

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.298 ± 0.019

5.882 ± 0.041

3.394 ± 0.022

3.666 ± 0.026

3.186 ± 0.027

6.394 ± 0.031

6.231 ± 0.045

6.365 ± 0.034

1.121 ± 0.015

4.407 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski