Amino acid dipepetide frequency for Mycobacterium phage Faze9

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
18.314AlaAla: 18.314 ± 1.567
1.456AlaCys: 1.456 ± 0.288
7.748AlaAsp: 7.748 ± 0.849
7.936AlaGlu: 7.936 ± 0.703
3.522AlaPhe: 3.522 ± 0.48
10.049AlaGly: 10.049 ± 0.941
2.536AlaHis: 2.536 ± 0.407
5.166AlaIle: 5.166 ± 0.61
4.039AlaLys: 4.039 ± 0.511
11.787AlaLeu: 11.787 ± 0.776
2.771AlaMet: 2.771 ± 0.383
4.273AlaAsn: 4.273 ± 0.415
7.514AlaPro: 7.514 ± 0.628
4.555AlaGln: 4.555 ± 0.378
8.734AlaArg: 8.734 ± 0.694
6.762AlaSer: 6.762 ± 0.687
8.828AlaThr: 8.828 ± 0.722
10.378AlaVal: 10.378 ± 0.714
2.16AlaTrp: 2.16 ± 0.301
3.005AlaTyr: 3.005 ± 0.38
0.0AlaXaa: 0.0 ± 0.0
Cys
1.456CysAla: 1.456 ± 0.324
0.094CysCys: 0.094 ± 0.072
0.892CysAsp: 0.892 ± 0.265
0.798CysGlu: 0.798 ± 0.189
0.141CysPhe: 0.141 ± 0.11
1.456CysGly: 1.456 ± 0.345
0.188CysHis: 0.188 ± 0.113
0.235CysIle: 0.235 ± 0.106
0.141CysLys: 0.141 ± 0.08
0.61CysLeu: 0.61 ± 0.163
0.235CysMet: 0.235 ± 0.103
0.376CysAsn: 0.376 ± 0.142
0.657CysPro: 0.657 ± 0.184
0.235CysGln: 0.235 ± 0.105
0.845CysArg: 0.845 ± 0.267
0.329CysSer: 0.329 ± 0.117
0.657CysThr: 0.657 ± 0.216
0.845CysVal: 0.845 ± 0.193
0.423CysTrp: 0.423 ± 0.189
0.235CysTyr: 0.235 ± 0.1
0.0CysXaa: 0.0 ± 0.0
Asp
7.42AspAla: 7.42 ± 0.59
0.704AspCys: 0.704 ± 0.194
4.837AspAsp: 4.837 ± 0.564
3.569AspGlu: 3.569 ± 0.418
1.55AspPhe: 1.55 ± 0.316
5.964AspGly: 5.964 ± 0.499
1.362AspHis: 1.362 ± 0.252
1.972AspIle: 1.972 ± 0.254
2.019AspLys: 2.019 ± 0.296
6.105AspLeu: 6.105 ± 0.629
1.784AspMet: 1.784 ± 0.325
1.737AspAsn: 1.737 ± 0.413
4.414AspPro: 4.414 ± 0.39
1.691AspGln: 1.691 ± 0.277
3.71AspArg: 3.71 ± 0.426
3.099AspSer: 3.099 ± 0.444
4.555AspThr: 4.555 ± 0.423
3.992AspVal: 3.992 ± 0.395
1.409AspTrp: 1.409 ± 0.253
1.315AspTyr: 1.315 ± 0.268
0.0AspXaa: 0.0 ± 0.0
Glu
8.171GluAla: 8.171 ± 0.882
0.564GluCys: 0.564 ± 0.163
3.428GluAsp: 3.428 ± 0.475
2.911GluGlu: 2.911 ± 0.414
2.207GluPhe: 2.207 ± 0.281
3.663GluGly: 3.663 ± 0.348
1.644GluHis: 1.644 ± 0.261
2.348GluIle: 2.348 ± 0.292
1.08GluLys: 1.08 ± 0.243
6.386GluLeu: 6.386 ± 0.542
0.892GluMet: 0.892 ± 0.186
1.268GluAsn: 1.268 ± 0.27
3.428GluPro: 3.428 ± 0.536
2.818GluGln: 2.818 ± 0.344
4.461GluArg: 4.461 ± 0.506
2.113GluSer: 2.113 ± 0.301
2.771GluThr: 2.771 ± 0.413
5.635GluVal: 5.635 ± 0.452
1.315GluTrp: 1.315 ± 0.24
1.174GluTyr: 1.174 ± 0.227
0.0GluXaa: 0.0 ± 0.0
Phe
3.898PheAla: 3.898 ± 0.395
0.094PheCys: 0.094 ± 0.063
1.878PheAsp: 1.878 ± 0.321
1.597PheGlu: 1.597 ± 0.266
0.517PhePhe: 0.517 ± 0.164
2.442PheGly: 2.442 ± 0.441
0.47PheHis: 0.47 ± 0.147
0.892PheIle: 0.892 ± 0.267
0.704PheLys: 0.704 ± 0.176
1.972PheLeu: 1.972 ± 0.342
0.376PheMet: 0.376 ± 0.138
1.127PheAsn: 1.127 ± 0.21
1.174PhePro: 1.174 ± 0.213
1.127PheGln: 1.127 ± 0.174
1.925PheArg: 1.925 ± 0.331
1.033PheSer: 1.033 ± 0.222
1.737PheThr: 1.737 ± 0.264
1.831PheVal: 1.831 ± 0.294
0.47PheTrp: 0.47 ± 0.16
0.47PheTyr: 0.47 ± 0.142
0.0PheXaa: 0.0 ± 0.0
Gly
8.312GlyAla: 8.312 ± 0.925
0.751GlyCys: 0.751 ± 0.232
5.447GlyAsp: 5.447 ± 0.44
5.4GlyGlu: 5.4 ± 0.516
2.301GlyPhe: 2.301 ± 0.406
10.472GlyGly: 10.472 ± 2.323
1.925GlyHis: 1.925 ± 0.341
3.757GlyIle: 3.757 ± 0.39
2.771GlyLys: 2.771 ± 0.44
7.701GlyLeu: 7.701 ± 0.792
1.784GlyMet: 1.784 ± 0.264
2.724GlyAsn: 2.724 ± 0.288
4.461GlyPro: 4.461 ± 0.589
3.945GlyGln: 3.945 ± 0.516
4.132GlyArg: 4.132 ± 0.556
4.226GlySer: 4.226 ± 0.514
7.185GlyThr: 7.185 ± 0.685
7.138GlyVal: 7.138 ± 0.642
2.16GlyTrp: 2.16 ± 0.313
2.583GlyTyr: 2.583 ± 0.434
0.0GlyXaa: 0.0 ± 0.0
His
2.113HisAla: 2.113 ± 0.424
0.282HisCys: 0.282 ± 0.148
1.221HisAsp: 1.221 ± 0.262
1.315HisGlu: 1.315 ± 0.246
0.61HisPhe: 0.61 ± 0.163
1.831HisGly: 1.831 ± 0.301
0.657HisHis: 0.657 ± 0.15
0.657HisIle: 0.657 ± 0.184
0.376HisLys: 0.376 ± 0.16
2.207HisLeu: 2.207 ± 0.392
0.423HisMet: 0.423 ± 0.146
0.657HisAsn: 0.657 ± 0.194
1.127HisPro: 1.127 ± 0.242
0.61HisGln: 0.61 ± 0.179
1.315HisArg: 1.315 ± 0.266
1.174HisSer: 1.174 ± 0.278
1.597HisThr: 1.597 ± 0.35
1.268HisVal: 1.268 ± 0.278
0.892HisTrp: 0.892 ± 0.221
0.47HisTyr: 0.47 ± 0.183
0.0HisXaa: 0.0 ± 0.0
Ile
5.494IleAla: 5.494 ± 0.358
0.235IleCys: 0.235 ± 0.113
3.052IleAsp: 3.052 ± 0.354
2.489IleGlu: 2.489 ± 0.355
0.423IlePhe: 0.423 ± 0.134
3.334IleGly: 3.334 ± 0.282
0.564IleHis: 0.564 ± 0.159
1.221IleIle: 1.221 ± 0.293
1.315IleLys: 1.315 ± 0.29
2.583IleLeu: 2.583 ± 0.308
0.704IleMet: 0.704 ± 0.177
1.362IleAsn: 1.362 ± 0.241
1.784IlePro: 1.784 ± 0.269
1.221IleGln: 1.221 ± 0.251
1.878IleArg: 1.878 ± 0.33
2.254IleSer: 2.254 ± 0.402
3.193IleThr: 3.193 ± 0.397
2.958IleVal: 2.958 ± 0.343
0.282IleTrp: 0.282 ± 0.129
0.423IleTyr: 0.423 ± 0.128
0.0IleXaa: 0.0 ± 0.0
Lys
4.273LysAla: 4.273 ± 0.614
0.423LysCys: 0.423 ± 0.136
1.268LysAsp: 1.268 ± 0.296
1.127LysGlu: 1.127 ± 0.224
0.657LysPhe: 0.657 ± 0.202
2.113LysGly: 2.113 ± 0.3
0.986LysHis: 0.986 ± 0.233
0.798LysIle: 0.798 ± 0.218
1.033LysLys: 1.033 ± 0.24
2.536LysLeu: 2.536 ± 0.319
0.564LysMet: 0.564 ± 0.135
0.845LysAsn: 0.845 ± 0.259
0.939LysPro: 0.939 ± 0.213
0.657LysGln: 0.657 ± 0.202
2.16LysArg: 2.16 ± 0.333
1.268LysSer: 1.268 ± 0.265
1.831LysThr: 1.831 ± 0.326
2.818LysVal: 2.818 ± 0.361
0.517LysTrp: 0.517 ± 0.147
0.61LysTyr: 0.61 ± 0.166
0.0LysXaa: 0.0 ± 0.0
Leu
13.994LeuAla: 13.994 ± 0.81
0.798LeuCys: 0.798 ± 0.187
5.212LeuAsp: 5.212 ± 0.585
4.743LeuGlu: 4.743 ± 0.492
1.737LeuPhe: 1.737 ± 0.265
7.044LeuGly: 7.044 ± 0.798
1.315LeuHis: 1.315 ± 0.24
3.71LeuIle: 3.71 ± 0.396
2.019LeuLys: 2.019 ± 0.328
7.185LeuLeu: 7.185 ± 0.538
2.066LeuMet: 2.066 ± 0.312
2.066LeuAsn: 2.066 ± 0.31
5.729LeuPro: 5.729 ± 0.568
2.254LeuGln: 2.254 ± 0.354
5.87LeuArg: 5.87 ± 0.459
4.508LeuSer: 4.508 ± 0.417
5.259LeuThr: 5.259 ± 0.457
6.574LeuVal: 6.574 ± 0.545
1.456LeuTrp: 1.456 ± 0.259
1.644LeuTyr: 1.644 ± 0.297
0.0LeuXaa: 0.0 ± 0.0
Met
3.616MetAla: 3.616 ± 0.401
0.141MetCys: 0.141 ± 0.087
1.174MetAsp: 1.174 ± 0.245
0.845MetGlu: 0.845 ± 0.224
0.47MetPhe: 0.47 ± 0.134
0.892MetGly: 0.892 ± 0.215
0.564MetHis: 0.564 ± 0.168
0.61MetIle: 0.61 ± 0.168
0.423MetLys: 0.423 ± 0.124
1.737MetLeu: 1.737 ± 0.361
0.235MetMet: 0.235 ± 0.098
0.61MetAsn: 0.61 ± 0.166
1.597MetPro: 1.597 ± 0.285
0.564MetGln: 0.564 ± 0.172
1.315MetArg: 1.315 ± 0.254
2.113MetSer: 2.113 ± 0.288
2.066MetThr: 2.066 ± 0.353
1.691MetVal: 1.691 ± 0.229
0.282MetTrp: 0.282 ± 0.129
0.423MetTyr: 0.423 ± 0.13
0.0MetXaa: 0.0 ± 0.0
Asn
3.945AsnAla: 3.945 ± 0.376
0.282AsnCys: 0.282 ± 0.124
1.691AsnAsp: 1.691 ± 0.303
1.268AsnGlu: 1.268 ± 0.267
0.564AsnPhe: 0.564 ± 0.148
3.757AsnGly: 3.757 ± 0.352
0.564AsnHis: 0.564 ± 0.179
1.221AsnIle: 1.221 ± 0.24
0.564AsnLys: 0.564 ± 0.171
2.442AsnLeu: 2.442 ± 0.294
0.704AsnMet: 0.704 ± 0.178
0.61AsnAsn: 0.61 ± 0.149
1.784AsnPro: 1.784 ± 0.285
1.174AsnGln: 1.174 ± 0.253
1.503AsnArg: 1.503 ± 0.265
1.597AsnSer: 1.597 ± 0.312
1.878AsnThr: 1.878 ± 0.368
1.831AsnVal: 1.831 ± 0.319
0.657AsnTrp: 0.657 ± 0.19
0.423AsnTyr: 0.423 ± 0.123
0.0AsnXaa: 0.0 ± 0.0
Pro
6.715ProAla: 6.715 ± 0.694
0.423ProCys: 0.423 ± 0.156
4.461ProAsp: 4.461 ± 0.389
5.306ProGlu: 5.306 ± 0.554
1.221ProPhe: 1.221 ± 0.233
6.527ProGly: 6.527 ± 0.756
1.221ProHis: 1.221 ± 0.307
1.597ProIle: 1.597 ± 0.361
1.737ProLys: 1.737 ± 0.344
3.71ProLeu: 3.71 ± 0.397
0.798ProMet: 0.798 ± 0.186
1.691ProAsn: 1.691 ± 0.28
4.508ProPro: 4.508 ± 0.63
1.55ProGln: 1.55 ± 0.285
2.536ProArg: 2.536 ± 0.334
3.193ProSer: 3.193 ± 0.405
5.025ProThr: 5.025 ± 0.512
6.34ProVal: 6.34 ± 0.497
0.892ProTrp: 0.892 ± 0.188
1.127ProTyr: 1.127 ± 0.224
0.0ProXaa: 0.0 ± 0.0
Gln
5.635GlnAla: 5.635 ± 0.526
0.188GlnCys: 0.188 ± 0.092
1.644GlnAsp: 1.644 ± 0.252
1.737GlnGlu: 1.737 ± 0.259
1.268GlnPhe: 1.268 ± 0.251
2.301GlnGly: 2.301 ± 0.326
0.564GlnHis: 0.564 ± 0.166
1.315GlnIle: 1.315 ± 0.289
0.657GlnLys: 0.657 ± 0.172
3.757GlnLeu: 3.757 ± 0.421
1.033GlnMet: 1.033 ± 0.217
0.564GlnAsn: 0.564 ± 0.172
1.55GlnPro: 1.55 ± 0.231
1.972GlnGln: 1.972 ± 0.327
2.818GlnArg: 2.818 ± 0.324
1.362GlnSer: 1.362 ± 0.256
1.691GlnThr: 1.691 ± 0.269
3.193GlnVal: 3.193 ± 0.395
1.033GlnTrp: 1.033 ± 0.231
0.657GlnTyr: 0.657 ± 0.156
0.0GlnXaa: 0.0 ± 0.0
Arg
7.983ArgAla: 7.983 ± 0.555
1.08ArgCys: 1.08 ± 0.233
3.945ArgAsp: 3.945 ± 0.47
4.039ArgGlu: 4.039 ± 0.598
1.784ArgPhe: 1.784 ± 0.284
5.072ArgGly: 5.072 ± 0.576
1.737ArgHis: 1.737 ± 0.28
2.442ArgIle: 2.442 ± 0.325
1.55ArgLys: 1.55 ± 0.333
5.87ArgLeu: 5.87 ± 0.498
1.878ArgMet: 1.878 ± 0.355
2.019ArgAsn: 2.019 ± 0.247
2.958ArgPro: 2.958 ± 0.383
2.348ArgGln: 2.348 ± 0.335
5.4ArgArg: 5.4 ± 0.57
2.818ArgSer: 2.818 ± 0.366
4.179ArgThr: 4.179 ± 0.397
4.461ArgVal: 4.461 ± 0.436
1.503ArgTrp: 1.503 ± 0.256
1.925ArgTyr: 1.925 ± 0.343
0.0ArgXaa: 0.0 ± 0.0
Ser
6.152SerAla: 6.152 ± 0.541
0.704SerCys: 0.704 ± 0.214
3.522SerAsp: 3.522 ± 0.441
2.63SerGlu: 2.63 ± 0.338
1.268SerPhe: 1.268 ± 0.248
6.105SerGly: 6.105 ± 0.504
0.564SerHis: 0.564 ± 0.179
1.831SerIle: 1.831 ± 0.316
1.784SerLys: 1.784 ± 0.299
3.804SerLeu: 3.804 ± 0.598
1.55SerMet: 1.55 ± 0.312
1.409SerAsn: 1.409 ± 0.282
3.146SerPro: 3.146 ± 0.365
1.362SerGln: 1.362 ± 0.249
2.677SerArg: 2.677 ± 0.361
2.489SerSer: 2.489 ± 0.418
3.522SerThr: 3.522 ± 0.417
3.945SerVal: 3.945 ± 0.472
0.986SerTrp: 0.986 ± 0.224
0.986SerTyr: 0.986 ± 0.262
0.0SerXaa: 0.0 ± 0.0
Thr
8.124ThrAla: 8.124 ± 0.615
0.751ThrCys: 0.751 ± 0.215
3.71ThrAsp: 3.71 ± 0.473
3.522ThrGlu: 3.522 ± 0.411
2.207ThrPhe: 2.207 ± 0.338
6.668ThrGly: 6.668 ± 0.615
1.409ThrHis: 1.409 ± 0.241
3.193ThrIle: 3.193 ± 0.376
2.489ThrLys: 2.489 ± 0.372
4.931ThrLeu: 4.931 ± 0.526
1.174ThrMet: 1.174 ± 0.228
1.831ThrAsn: 1.831 ± 0.288
5.964ThrPro: 5.964 ± 0.634
2.395ThrGln: 2.395 ± 0.429
3.475ThrArg: 3.475 ± 0.372
3.898ThrSer: 3.898 ± 0.518
4.696ThrThr: 4.696 ± 0.542
5.212ThrVal: 5.212 ± 0.564
1.597ThrTrp: 1.597 ± 0.316
1.644ThrTyr: 1.644 ± 0.257
0.0ThrXaa: 0.0 ± 0.0
Val
11.411ValAla: 11.411 ± 0.871
1.033ValCys: 1.033 ± 0.223
5.259ValAsp: 5.259 ± 0.434
4.555ValGlu: 4.555 ± 0.5
1.831ValPhe: 1.831 ± 0.252
6.105ValGly: 6.105 ± 0.661
1.503ValHis: 1.503 ± 0.306
2.489ValIle: 2.489 ± 0.325
1.597ValLys: 1.597 ± 0.275
6.386ValLeu: 6.386 ± 0.515
1.503ValMet: 1.503 ± 0.328
1.972ValAsn: 1.972 ± 0.259
5.588ValPro: 5.588 ± 0.58
2.958ValGln: 2.958 ± 0.34
6.293ValArg: 6.293 ± 0.727
4.273ValSer: 4.273 ± 0.573
5.212ValThr: 5.212 ± 0.524
6.34ValVal: 6.34 ± 0.704
1.221ValTrp: 1.221 ± 0.27
1.831ValTyr: 1.831 ± 0.326
0.0ValXaa: 0.0 ± 0.0
Trp
2.207TrpAla: 2.207 ± 0.439
0.517TrpCys: 0.517 ± 0.181
1.174TrpAsp: 1.174 ± 0.166
1.268TrpGlu: 1.268 ± 0.22
0.845TrpPhe: 0.845 ± 0.218
1.033TrpGly: 1.033 ± 0.257
0.751TrpHis: 0.751 ± 0.191
0.564TrpIle: 0.564 ± 0.13
0.423TrpLys: 0.423 ± 0.128
1.784TrpLeu: 1.784 ± 0.297
0.376TrpMet: 0.376 ± 0.124
0.657TrpAsn: 0.657 ± 0.186
1.221TrpPro: 1.221 ± 0.226
0.798TrpGln: 0.798 ± 0.195
1.409TrpArg: 1.409 ± 0.234
1.409TrpSer: 1.409 ± 0.302
1.127TrpThr: 1.127 ± 0.263
1.315TrpVal: 1.315 ± 0.27
0.517TrpTrp: 0.517 ± 0.174
0.61TrpTyr: 0.61 ± 0.175
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.348TyrAla: 2.348 ± 0.334
0.329TyrCys: 0.329 ± 0.121
1.644TyrAsp: 1.644 ± 0.303
1.221TyrGlu: 1.221 ± 0.239
0.61TyrPhe: 0.61 ± 0.171
2.348TyrGly: 2.348 ± 0.278
0.282TyrHis: 0.282 ± 0.104
0.751TyrIle: 0.751 ± 0.17
0.704TyrLys: 0.704 ± 0.175
1.691TyrLeu: 1.691 ± 0.342
0.47TyrMet: 0.47 ± 0.147
0.657TyrAsn: 0.657 ± 0.18
1.033TyrPro: 1.033 ± 0.221
0.704TyrGln: 0.704 ± 0.188
2.536TyrArg: 2.536 ± 0.359
0.517TyrSer: 0.517 ± 0.141
1.878TyrThr: 1.878 ± 0.307
1.597TyrVal: 1.597 ± 0.28
0.235TyrTrp: 0.235 ± 0.101
0.47TyrTyr: 0.47 ± 0.162
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 91 proteins (21296 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski