Firmicutes bacterium CAG:41

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; environmental samples

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2447 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R6MM19|R6MM19_9FIRM Uncharacterized protein OS=Firmicutes bacterium CAG:41 OX=1263021 GN=BN647_01303 PE=4 SV=1
MM1 pKa = 8.24DD2 pKa = 3.78YY3 pKa = 11.55AKK5 pKa = 10.66FEE7 pKa = 4.32NFQEE11 pKa = 4.78FYY13 pKa = 11.05DD14 pKa = 4.0AVEE17 pKa = 4.12MGLDD21 pKa = 3.07IEE23 pKa = 4.65FFIDD27 pKa = 4.59GIRR30 pKa = 11.84YY31 pKa = 8.81NISWQNNKK39 pKa = 9.3PFICVCPDD47 pKa = 3.54GEE49 pKa = 4.31PNYY52 pKa = 10.96YY53 pKa = 10.61SDD55 pKa = 4.51TQDD58 pKa = 4.66LLDD61 pKa = 3.42NHH63 pKa = 7.13KK64 pKa = 10.39IDD66 pKa = 4.26GVPIKK71 pKa = 10.44DD72 pKa = 3.1LWQNIDD78 pKa = 3.04IYY80 pKa = 11.9AMM82 pKa = 3.95

Molecular weight: 9.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R6MUC6|R6MUC6_9FIRM Rubredoxin OS=Firmicutes bacterium CAG:41 OX=1263021 GN=BN647_02394 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.67KK9 pKa = 7.77RR10 pKa = 11.84QRR12 pKa = 11.84AKK14 pKa = 9.48EE15 pKa = 3.53HH16 pKa = 5.95GFRR19 pKa = 11.84KK20 pKa = 10.03RR21 pKa = 11.84MSTKK25 pKa = 10.02SGRR28 pKa = 11.84KK29 pKa = 8.25ILASRR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.59GRR39 pKa = 11.84KK40 pKa = 8.7KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2447

0

2447

776536

31

3443

317.3

35.56

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.788 ± 0.051

1.397 ± 0.022

6.464 ± 0.045

7.08 ± 0.054

4.034 ± 0.035

6.758 ± 0.052

1.493 ± 0.02

8.185 ± 0.052

7.838 ± 0.051

7.649 ± 0.051

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.906 ± 0.026

5.778 ± 0.042

3.023 ± 0.028

2.601 ± 0.023

3.57 ± 0.041

6.255 ± 0.053

6.039 ± 0.072

6.979 ± 0.044

0.837 ± 0.016

4.328 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski