Furfurilactobacillus siliginis
Average proteome isoelectric point is 6.35
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1922 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0R2L731|A0A0R2L731_9LACO 50S ribosomal protein L20 OS=Furfurilactobacillus siliginis OX=348151 GN=rplT PE=3 SV=1
MM1 pKa = 7.3 AQYY4 pKa = 11.05 KK5 pKa = 9.64 VIQDD9 pKa = 3.67 FTDD12 pKa = 3.92 SNDD15 pKa = 3.03 KK16 pKa = 11.13 SADD19 pKa = 3.57 ANGDD23 pKa = 3.5 LHH25 pKa = 7.24 VYY27 pKa = 9.93 RR28 pKa = 11.84 AGDD31 pKa = 3.91 AYY33 pKa = 10.01 PFQPYY38 pKa = 10.48 AGAQTADD45 pKa = 4.39 RR46 pKa = 11.84 IALLTSYY53 pKa = 10.68 DD54 pKa = 3.79 GPNDD58 pKa = 4.2 DD59 pKa = 4.66 FDD61 pKa = 5.88 GPVIEE66 pKa = 4.89 QLTDD70 pKa = 2.89
Molecular weight: 7.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.745
IPC_protein 3.719
Toseland 3.478
ProMoST 3.859
Dawson 3.757
Bjellqvist 3.986
Wikipedia 3.77
Rodwell 3.554
Grimsley 3.401
Solomon 3.732
Lehninger 3.694
Nozaki 3.884
DTASelect 4.202
Thurlkill 3.579
EMBOSS 3.77
Sillero 3.859
Patrickios 1.914
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.819
Protein with the highest isoelectric point:
>tr|A0A0R2L3A2|A0A0R2L3A2_9LACO Global transcriptional regulator Spx OS=Furfurilactobacillus siliginis OX=348151 GN=arsC PE=3 SV=1
MM1 pKa = 7.44 TNAEE5 pKa = 4.12 LQQLVEE11 pKa = 4.62 TISLRR16 pKa = 11.84 DD17 pKa = 3.52 FNRR20 pKa = 11.84 PFKK23 pKa = 10.77 HH24 pKa = 5.73 RR25 pKa = 11.84 AIFNARR31 pKa = 11.84 LRR33 pKa = 11.84 TTGGRR38 pKa = 11.84 YY39 pKa = 9.51 HH40 pKa = 7.8 LDD42 pKa = 3.2 DD43 pKa = 3.63 HH44 pKa = 7.35 HH45 pKa = 8.96 IDD47 pKa = 3.65 INPLMLTDD55 pKa = 4.64 FDD57 pKa = 4.0 EE58 pKa = 4.73 TNLIGVIRR66 pKa = 11.84 HH67 pKa = 5.56 EE68 pKa = 4.12 LVHH71 pKa = 5.65 YY72 pKa = 10.34 HH73 pKa = 6.77 LALAGLPFSHH83 pKa = 7.94 RR84 pKa = 11.84 SRR86 pKa = 11.84 QFKK89 pKa = 10.11 QLLTKK94 pKa = 10.07 VHH96 pKa = 6.76 GSRR99 pKa = 11.84 FAPAPKK105 pKa = 10.13 KK106 pKa = 10.15 PQTKK110 pKa = 9.41 RR111 pKa = 11.84 LAVRR115 pKa = 11.84 YY116 pKa = 9.09 IYY118 pKa = 10.44 EE119 pKa = 4.11 CEE121 pKa = 3.66 RR122 pKa = 11.84 CGRR125 pKa = 11.84 RR126 pKa = 11.84 FVRR129 pKa = 11.84 QRR131 pKa = 11.84 RR132 pKa = 11.84 VNVRR136 pKa = 11.84 KK137 pKa = 8.77 FACGACGGHH146 pKa = 6.63 LHH148 pKa = 6.06 LQEE151 pKa = 4.19 SSKK154 pKa = 11.14 LII156 pKa = 3.98
Molecular weight: 18.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.349
IPC2_protein 9.589
IPC_protein 10.321
Toseland 10.599
ProMoST 10.277
Dawson 10.701
Bjellqvist 10.409
Wikipedia 10.906
Rodwell 10.906
Grimsley 10.745
Solomon 10.804
Lehninger 10.774
Nozaki 10.599
DTASelect 10.394
Thurlkill 10.599
EMBOSS 10.994
Sillero 10.643
Patrickios 10.643
IPC_peptide 10.804
IPC2_peptide 9.56
IPC2.peptide.svr19 8.564
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1922
0
1922
571064
50
3987
297.1
32.87
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.309 ± 0.122
0.361 ± 0.012
6.14 ± 0.058
5.1 ± 0.069
4.148 ± 0.045
6.8 ± 0.058
2.324 ± 0.036
6.261 ± 0.055
5.211 ± 0.061
9.836 ± 0.074
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.708 ± 0.03
4.477 ± 0.044
3.712 ± 0.049
4.842 ± 0.061
4.279 ± 0.057
5.694 ± 0.079
6.84 ± 0.081
7.573 ± 0.048
1.134 ± 0.028
3.191 ± 0.038
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here