Armatimonadetes bacterium DC
Average proteome isoelectric point is 6.62
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2575 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0S4PMW0|A0A0S4PMW0_9BACT RNA polymerase sigma-70 factor ECF subfamily OS=Armatimonadetes bacterium DC OX=1661583 GN=DCOP10_12075 PE=3 SV=1
MM1 pKa = 7.5 QDD3 pKa = 3.42 LGTLGGSQSAAYY15 pKa = 9.75 DD16 pKa = 3.39 VSADD20 pKa = 3.37 GNVIVGQAEE29 pKa = 3.79 NDD31 pKa = 4.08 GYY33 pKa = 9.51 QWRR36 pKa = 11.84 PFRR39 pKa = 11.84 WTPAGGVEE47 pKa = 5.44 DD48 pKa = 5.43 LNQTYY53 pKa = 10.95 ASLLTGGSEE62 pKa = 3.81 LWEE65 pKa = 3.93 AHH67 pKa = 6.75 AISPDD72 pKa = 2.63 GRR74 pKa = 11.84 YY75 pKa = 9.15 IVGFGYY81 pKa = 10.63 NAATDD86 pKa = 3.7 RR87 pKa = 11.84 DD88 pKa = 4.03 EE89 pKa = 6.22 AFLLDD94 pKa = 3.36 TWRR97 pKa = 11.84 TGDD100 pKa = 3.7 TNGDD104 pKa = 3.54 GCIDD108 pKa = 4.1 DD109 pKa = 5.47 ADD111 pKa = 4.13 LLAVLFAFGTPGSGLTCHH129 pKa = 6.8 EE130 pKa = 5.94 DD131 pKa = 3.1 INKK134 pKa = 10.65 DD135 pKa = 3.63 GVVDD139 pKa = 4.49 DD140 pKa = 5.39 ADD142 pKa = 4.07 LLTVLFNFGSGCC154 pKa = 3.69
Molecular weight: 16.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.698
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.427
ProMoST 3.859
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.656
Rodwell 3.49
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.795
DTASelect 4.075
Thurlkill 3.503
EMBOSS 3.656
Sillero 3.783
Patrickios 0.947
IPC_peptide 3.656
IPC2_peptide 3.757
IPC2.peptide.svr19 3.73
Protein with the highest isoelectric point:
>tr|A0A0S4PJP5|A0A0S4PJP5_9BACT Uncharacterized protein OS=Armatimonadetes bacterium DC OX=1661583 GN=DCOP10_116282 PE=3 SV=1
MM1 pKa = 7.46 KK2 pKa = 9.26 RR3 pKa = 11.84 TNLLRR8 pKa = 11.84 WVRR11 pKa = 11.84 RR12 pKa = 11.84 TWAQGTVGKK21 pKa = 8.09 TVATVLLVAMVMPSFAGVSWRR42 pKa = 11.84 TAFRR46 pKa = 11.84 GGGKK50 pKa = 9.79
Molecular weight: 5.55 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.443
IPC2_protein 10.95
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.188
Rodwell 12.398
Grimsley 12.749
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 12.149
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.09
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2575
0
2575
877366
39
2207
340.7
38.01
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.811 ± 0.056
0.945 ± 0.018
4.387 ± 0.033
6.88 ± 0.059
3.583 ± 0.031
7.64 ± 0.061
2.124 ± 0.024
5.007 ± 0.035
2.866 ± 0.039
10.975 ± 0.064
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.172 ± 0.02
2.848 ± 0.033
5.869 ± 0.035
4.485 ± 0.043
7.69 ± 0.038
4.724 ± 0.04
5.292 ± 0.036
7.586 ± 0.043
1.896 ± 0.03
3.222 ± 0.033
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here