Desulfofustis glycolicus DSM 9705

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobulbaceae; Desulfofustis; Desulfofustis glycolicus

Average proteome isoelectric point is 6.42

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4455 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1M5VTD3|A0A1M5VTD3_9DELT 2-isopropylmalate synthase OS=Desulfofustis glycolicus DSM 9705 OX=1121409 GN=leuA PE=3 SV=1
MM1 pKa = 7.04ITLLLFALSVIPLQLTYY18 pKa = 11.13QNDD21 pKa = 3.99TKK23 pKa = 11.01DD24 pKa = 3.62LQSAISTIQHH34 pKa = 6.24LQQTNQSADD43 pKa = 3.18SSFQTAFTDD52 pKa = 4.54TITALQTAAGLATLATLFTTDD73 pKa = 4.93SAPQDD78 pKa = 3.7ADD80 pKa = 3.53TQQIVIPVRR89 pKa = 11.84LPCLLPSTITVGSDD103 pKa = 3.0LSIQKK108 pKa = 10.4LSPDD112 pKa = 3.72PYY114 pKa = 9.87QFVTISYY121 pKa = 10.1LPIPDD126 pKa = 3.88TPPPLFCC133 pKa = 6.43

Molecular weight: 14.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1M5V790|A0A1M5V790_9DELT Cytochrome c mono-and diheme variants OS=Desulfofustis glycolicus DSM 9705 OX=1121409 GN=SAMN02745124_01547 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSNIKK12 pKa = 9.92RR13 pKa = 11.84KK14 pKa = 8.68RR15 pKa = 11.84RR16 pKa = 11.84HH17 pKa = 4.73GFRR20 pKa = 11.84EE21 pKa = 3.92RR22 pKa = 11.84MSTKK26 pKa = 10.22AGRR29 pKa = 11.84AIINARR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.13GRR40 pKa = 11.84KK41 pKa = 8.18QLSAA45 pKa = 3.9

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4455

0

4455

1448063

39

3212

325.0

35.95

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.278 ± 0.039

1.334 ± 0.018

5.652 ± 0.032

6.156 ± 0.033

4.089 ± 0.026

7.746 ± 0.032

2.182 ± 0.016

6.322 ± 0.029

3.947 ± 0.034

10.718 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.386 ± 0.016

3.051 ± 0.022

4.554 ± 0.019

3.736 ± 0.025

6.591 ± 0.034

5.842 ± 0.023

5.419 ± 0.026

7.081 ± 0.029

1.12 ± 0.014

2.799 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski