Bordetella genomosp. 9
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4089 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1W6Z2N7|A0A1W6Z2N7_9BORD Uncharacterized protein OS=Bordetella genomosp. 9 OX=1416803 GN=CAL13_16565 PE=4 SV=1
MM1 pKa = 6.51 THH3 pKa = 6.28 VVTEE7 pKa = 3.94 NCIKK11 pKa = 10.53 CKK13 pKa = 10.04 YY14 pKa = 7.62 TDD16 pKa = 4.08 CVDD19 pKa = 3.61 VCPVDD24 pKa = 4.18 CFRR27 pKa = 11.84 EE28 pKa = 4.9 GPNFLVIDD36 pKa = 4.26 PDD38 pKa = 3.65 EE39 pKa = 5.79 CIDD42 pKa = 3.87 CAVCIPEE49 pKa = 4.56 CPANAIYY56 pKa = 10.74 AEE58 pKa = 4.15 EE59 pKa = 5.28 DD60 pKa = 3.68 VPQDD64 pKa = 3.12 QLQFIKK70 pKa = 10.9 LNAEE74 pKa = 3.91 LATEE78 pKa = 4.67 FTSISRR84 pKa = 11.84 AKK86 pKa = 10.48 KK87 pKa = 9.8 PLDD90 pKa = 5.53 DD91 pKa = 4.49 ADD93 pKa = 3.74 EE94 pKa = 4.53 WNGKK98 pKa = 7.58 PDD100 pKa = 3.28 KK101 pKa = 10.81 LQYY104 pKa = 10.42 LEE106 pKa = 4.32 KK107 pKa = 10.85
Molecular weight: 12.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.899
IPC2_protein 4.177
IPC_protein 4.113
Toseland 3.923
ProMoST 4.253
Dawson 4.088
Bjellqvist 4.24
Wikipedia 3.999
Rodwell 3.948
Grimsley 3.834
Solomon 4.088
Lehninger 4.037
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.012
Sillero 4.228
Patrickios 1.074
IPC_peptide 4.088
IPC2_peptide 4.215
IPC2.peptide.svr19 4.122
Protein with the highest isoelectric point:
>tr|A0A1W6Z4Z8|A0A1W6Z4Z8_9BORD ABC transporter ATP-binding protein OS=Bordetella genomosp. 9 OX=1416803 GN=CAL13_00530 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.51 RR3 pKa = 11.84 TFQPSVTRR11 pKa = 11.84 RR12 pKa = 11.84 KK13 pKa = 8.0 RR14 pKa = 11.84 THH16 pKa = 5.76 GFRR19 pKa = 11.84 VRR21 pKa = 11.84 MKK23 pKa = 9.39 TRR25 pKa = 11.84 GGRR28 pKa = 11.84 AVLNARR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.65 GRR39 pKa = 11.84 KK40 pKa = 8.75 RR41 pKa = 11.84 LAVV44 pKa = 3.41
Molecular weight: 5.19 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.286
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.705
Grimsley 13.086
Solomon 13.539
Lehninger 13.451
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.427
IPC_peptide 13.554
IPC2_peptide 12.53
IPC2.peptide.svr19 9.21
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4089
0
4089
1354728
29
2996
331.3
35.9
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
13.317 ± 0.061
0.9 ± 0.012
5.522 ± 0.031
5.018 ± 0.042
3.321 ± 0.02
8.578 ± 0.039
2.198 ± 0.018
4.509 ± 0.025
2.74 ± 0.034
10.387 ± 0.049
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.526 ± 0.02
2.513 ± 0.025
5.638 ± 0.029
3.698 ± 0.024
7.692 ± 0.042
5.053 ± 0.027
5.014 ± 0.034
7.578 ± 0.03
1.415 ± 0.017
2.381 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here