Mycobacterium phage Papez
Average proteome isoelectric point is 6.06
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 94 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1B1PCW2|A0A1B1PCW2_9CAUD Uncharacterized protein OS=Mycobacterium phage Papez OX=1873891 GN=1 PE=4 SV=1
MM1 pKa = 7.61 GFTLRR6 pKa = 11.84 LFGIPVLSLEE16 pKa = 4.13 ITGDD20 pKa = 3.64 GSAEE24 pKa = 4.06 EE25 pKa = 4.65 YY26 pKa = 10.37 ISLTGGSFEE35 pKa = 4.74 LAPEE39 pKa = 3.98 EE40 pKa = 4.15 PEE42 pKa = 3.9 YY43 pKa = 11.13 DD44 pKa = 3.29 EE45 pKa = 5.61 EE46 pKa = 4.95 YY47 pKa = 10.82 YY48 pKa = 10.93 EE49 pKa = 4.22 EE50 pKa = 4.6 DD51 pKa = 3.1 RR52 pKa = 11.84 SGFGFGVSS60 pKa = 3.28
Molecular weight: 6.61 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.891
IPC2_protein 3.719
IPC_protein 3.528
Toseland 3.389
ProMoST 3.681
Dawson 3.49
Bjellqvist 3.668
Wikipedia 3.401
Rodwell 3.389
Grimsley 3.325
Solomon 3.439
Lehninger 3.389
Nozaki 3.656
DTASelect 3.668
Thurlkill 3.452
EMBOSS 3.414
Sillero 3.643
Patrickios 0.477
IPC_peptide 3.439
IPC2_peptide 3.617
IPC2.peptide.svr19 3.664
Protein with the highest isoelectric point:
>tr|A0A1B1PCW0|A0A1B1PCW0_9CAUD Capsid maturation protease OS=Mycobacterium phage Papez OX=1873891 GN=15 PE=4 SV=1
MM1 pKa = 7.3 GAAHH5 pKa = 7.34 LPACSYY11 pKa = 10.58 QLQDD15 pKa = 2.67 RR16 pKa = 11.84 GMKK19 pKa = 9.82 RR20 pKa = 11.84 VRR22 pKa = 11.84 EE23 pKa = 3.92 LVLIRR28 pKa = 11.84 MLDD31 pKa = 3.28 HH32 pKa = 6.78 EE33 pKa = 4.97 VRR35 pKa = 11.84 LEE37 pKa = 3.73 HH38 pKa = 7.22 LIQIMRR44 pKa = 11.84 GWFRR48 pKa = 3.52
Molecular weight: 5.77 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.279
IPC2_protein 9.18
IPC_protein 9.984
Toseland 10.014
ProMoST 10.687
Dawson 10.218
Bjellqvist 10.014
Wikipedia 10.452
Rodwell 10.248
Grimsley 10.292
Solomon 10.394
Lehninger 10.365
Nozaki 10.116
DTASelect 9.984
Thurlkill 10.072
EMBOSS 10.423
Sillero 10.175
Patrickios 10.292
IPC_peptide 10.379
IPC2_peptide 9.355
IPC2.peptide.svr19 8.543
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
94
0
94
16495
25
826
175.5
19.29
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.694 ± 0.386
0.649 ± 0.111
6.547 ± 0.224
6.699 ± 0.275
3.195 ± 0.189
8.706 ± 0.518
2.043 ± 0.188
4.905 ± 0.215
4.129 ± 0.247
8.669 ± 0.233
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.934 ± 0.122
3.328 ± 0.145
5.511 ± 0.233
3.219 ± 0.175
6.208 ± 0.42
6.014 ± 0.307
6.25 ± 0.323
7.317 ± 0.208
2.098 ± 0.129
2.886 ± 0.193
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here