Rhizopus azygosporus (Rhizopus microsporus var. azygosporus)
Average proteome isoelectric point is 6.58
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 15771 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A367IPX5|A0A367IPX5_RHIAZ Uncharacterized protein (Fragment) OS=Rhizopus azygosporus OX=86630 GN=CU097_001865 PE=4 SV=1
PP1 pKa = 7.01 VAFEE5 pKa = 4.84 DD6 pKa = 4.97 FEE8 pKa = 4.45 FTLGYY13 pKa = 10.84 DD14 pKa = 3.68 FAVPIDD20 pKa = 3.92 FNEE23 pKa = 4.71 LEE25 pKa = 5.26 DD26 pKa = 4.19 IPFNPEE32 pKa = 3.31 AHH34 pKa = 5.83 SHH36 pKa = 5.88 VLDD39 pKa = 3.78 EE40 pKa = 5.56 DD41 pKa = 3.57 EE42 pKa = 6.09 RR43 pKa = 11.84 DD44 pKa = 3.51 MFNQFLDD51 pKa = 3.58 QFDD54 pKa = 4.08 TDD56 pKa = 4.57 GDD58 pKa = 3.5 IHH60 pKa = 6.69 MDD62 pKa = 3.81 PSAFSFPVQVDD73 pKa = 3.33 EE74 pKa = 6.64 DD75 pKa = 4.46 GLCEE79 pKa = 4.32 SFLLHH84 pKa = 7.35 SLDD87 pKa = 4.06 DD88 pKa = 4.1 RR89 pKa = 11.84 NNVIEE94 pKa = 5.07 DD95 pKa = 3.34 NSEE98 pKa = 4.2 YY99 pKa = 10.98 EE100 pKa = 4.27 HH101 pKa = 6.67 GASLLKK107 pKa = 10.51 EE108 pKa = 3.82 ATNRR112 pKa = 11.84 LGYY115 pKa = 10.19 LCSSTQSTGNISSGLNTSNTNHH137 pKa = 6.19 YY138 pKa = 10.93 GEE140 pKa = 4.91 LTVPMM145 pKa = 5.3
Molecular weight: 16.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.705
IPC2_protein 3.783
IPC_protein 3.77
Toseland 3.554
ProMoST 3.923
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.681
Rodwell 3.592
Grimsley 3.465
Solomon 3.745
Lehninger 3.694
Nozaki 3.872
DTASelect 4.088
Thurlkill 3.605
EMBOSS 3.694
Sillero 3.884
Patrickios 1.036
IPC_peptide 3.745
IPC2_peptide 3.859
IPC2.peptide.svr19 3.794
Protein with the highest isoelectric point:
>tr|A0A367JRL9|A0A367JRL9_RHIAZ Uncharacterized protein OS=Rhizopus azygosporus OX=86630 GN=CU097_012938 PE=4 SV=1
MM1 pKa = 7.57 FGQLLTTATLRR12 pKa = 11.84 FTSLTNAVRR21 pKa = 11.84 PTLMGQSMFSNPLSNVLTTQMRR43 pKa = 11.84 FVTRR47 pKa = 11.84 GNTYY51 pKa = 10.12 QPSQLVRR58 pKa = 11.84 KK59 pKa = 9.04 RR60 pKa = 11.84 RR61 pKa = 11.84 HH62 pKa = 5.16 GFLTRR67 pKa = 11.84 LATKK71 pKa = 10.26 NGRR74 pKa = 11.84 HH75 pKa = 5.88 IINRR79 pKa = 11.84 RR80 pKa = 11.84 RR81 pKa = 11.84 MKK83 pKa = 10.08 GRR85 pKa = 11.84 KK86 pKa = 8.57 FLSHH90 pKa = 6.98
Molecular weight: 10.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.463
IPC2_protein 11.184
IPC_protein 12.588
Toseland 12.749
ProMoST 13.247
Dawson 12.749
Bjellqvist 12.749
Wikipedia 13.217
Rodwell 12.384
Grimsley 12.793
Solomon 13.247
Lehninger 13.144
Nozaki 12.749
DTASelect 12.749
Thurlkill 12.749
EMBOSS 13.247
Sillero 12.749
Patrickios 12.106
IPC_peptide 13.247
IPC2_peptide 12.237
IPC2.peptide.svr19 9.106
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
15771
0
15771
6121853
50
5889
388.2
43.98
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.442 ± 0.016
1.409 ± 0.009
5.715 ± 0.015
6.701 ± 0.022
3.993 ± 0.013
4.88 ± 0.017
2.533 ± 0.009
6.342 ± 0.016
6.639 ± 0.021
9.216 ± 0.022
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.42 ± 0.008
4.836 ± 0.014
4.743 ± 0.017
4.623 ± 0.015
4.97 ± 0.015
7.906 ± 0.024
6.031 ± 0.014
5.906 ± 0.014
1.16 ± 0.006
3.534 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here