Helicobacter muridarum

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Epsilonproteobacteria; Campylobacterales; Helicobacteraceae;

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1960 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A099TZ24|A0A099TZ24_9HELI TPR repeat-containing protein OS=Helicobacter muridarum OX=216 GN=LS73_005840 PE=3 SV=1
MM1 pKa = 7.64IEE3 pKa = 4.14NLKK6 pKa = 10.03SHH8 pKa = 5.18YY9 pKa = 10.45HH10 pKa = 6.6EE11 pKa = 4.82ILFSLDD17 pKa = 3.28CGNDD21 pKa = 3.25VLDD24 pKa = 5.63EE25 pKa = 4.26IVCQIIVDD33 pKa = 4.54KK34 pKa = 11.16ILDD37 pKa = 3.64NGEE40 pKa = 3.99AVEE43 pKa = 4.56FLSSDD48 pKa = 3.36MVDD51 pKa = 2.83VCNFDD56 pKa = 5.03DD57 pKa = 5.67EE58 pKa = 5.29VILDD62 pKa = 3.69

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A099TXV0|A0A099TXV0_9HELI Methyltransferase OS=Helicobacter muridarum OX=216 GN=dpnA_3 PE=3 SV=1
MM1 pKa = 7.28KK2 pKa = 9.44RR3 pKa = 11.84TYY5 pKa = 10.04QPHH8 pKa = 4.86NTPRR12 pKa = 11.84KK13 pKa = 7.31RR14 pKa = 11.84THH16 pKa = 5.88GFRR19 pKa = 11.84VRR21 pKa = 11.84MKK23 pKa = 8.63TKK25 pKa = 9.91NGRR28 pKa = 11.84RR29 pKa = 11.84VINARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.73GRR39 pKa = 11.84KK40 pKa = 8.53ALSVAA45 pKa = 3.78

Molecular weight: 5.33 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1960

0

1960

636876

29

2887

324.9

36.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.136 ± 0.046

1.288 ± 0.027

5.451 ± 0.047

5.783 ± 0.058

5.104 ± 0.044

5.664 ± 0.061

1.893 ± 0.025

9.619 ± 0.054

7.754 ± 0.063

9.999 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.369 ± 0.024

6.585 ± 0.053

2.922 ± 0.023

3.749 ± 0.039

3.693 ± 0.032

7.734 ± 0.046

4.369 ± 0.035

5.079 ± 0.049

0.713 ± 0.013

4.096 ± 0.035
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski