Salmonella phage SPN9CC

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Lederbergvirus; unclassified Lederbergvirus

Average proteome isoelectric point is 7.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 62 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I1TEH1|I1TEH1_9CAUD Uncharacterized protein OS=Salmonella phage SPN9CC OX=1127357 GN=SPN9CC_0035 PE=4 SV=1
MM1 pKa = 7.71LSPSQYY7 pKa = 10.65LQYY10 pKa = 10.59QKK12 pKa = 11.15EE13 pKa = 4.45SVEE16 pKa = 4.33RR17 pKa = 11.84ALTCANCGQKK27 pKa = 10.32LHH29 pKa = 5.86VLEE32 pKa = 4.31VHH34 pKa = 5.94VCEE37 pKa = 4.54HH38 pKa = 6.71CCAEE42 pKa = 4.99LMSDD46 pKa = 4.35PNSSMYY52 pKa = 10.6EE53 pKa = 3.98EE54 pKa = 5.0EE55 pKa = 4.99DD56 pKa = 4.39DD57 pKa = 4.1EE58 pKa = 4.75

Molecular weight: 6.65 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I1TEF4|I1TEF4_9CAUD Uncharacterized protein OS=Salmonella phage SPN9CC OX=1127357 GN=SPN9CC_0018 PE=4 SV=1
MM1 pKa = 7.26TRR3 pKa = 11.84RR4 pKa = 11.84TQFKK8 pKa = 7.85GTSRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84RR16 pKa = 11.84EE17 pKa = 3.84RR18 pKa = 11.84LKK20 pKa = 11.2AKK22 pKa = 10.38ALANGVLARR31 pKa = 11.84EE32 pKa = 4.08EE33 pKa = 4.91AITSEE38 pKa = 4.28VLHH41 pKa = 6.71RR42 pKa = 11.84PTLSRR47 pKa = 11.84AQIQAKK53 pKa = 9.67GKK55 pKa = 9.64HH56 pKa = 4.86EE57 pKa = 4.14TPKK60 pKa = 10.42RR61 pKa = 11.84IEE63 pKa = 4.21DD64 pKa = 3.59AKK66 pKa = 11.04SLQFMAKK73 pKa = 9.92DD74 pKa = 3.48AFWQLEE80 pKa = 4.22EE81 pKa = 3.9YY82 pKa = 10.34RR83 pKa = 11.84RR84 pKa = 11.84NLEE87 pKa = 3.59RR88 pKa = 11.84AAIVYY93 pKa = 10.58ANEE96 pKa = 4.43FGHH99 pKa = 6.59KK100 pKa = 9.71PPEE103 pKa = 4.36TGVCLPDD110 pKa = 3.32VALYY114 pKa = 10.48AAGHH118 pKa = 6.21RR119 pKa = 11.84KK120 pKa = 9.32SKK122 pKa = 10.8QITARR127 pKa = 3.53

Molecular weight: 14.53 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

62

0

62

12099

27

724

195.1

21.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.257 ± 0.448

1.174 ± 0.154

5.695 ± 0.309

6.232 ± 0.355

3.595 ± 0.274

7.149 ± 0.433

1.62 ± 0.188

6.505 ± 0.412

6.083 ± 0.31

7.935 ± 0.294

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.992 ± 0.168

4.538 ± 0.232

4.0 ± 0.185

4.794 ± 0.41

6.1 ± 0.303

6.257 ± 0.287

5.108 ± 0.289

6.058 ± 0.228

1.488 ± 0.184

3.422 ± 0.226
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski