Rhizobium phage vB_RleM_P10VF

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Ackermannviridae; unclassified Ackermannviridae

Average proteome isoelectric point is 6.14

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 257 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A076YNQ5|A0A076YNQ5_9CAUD Uncharacterized protein OS=Rhizobium phage vB_RleM_P10VF OX=1527770 GN=P10VF_226 PE=4 SV=1
MM1 pKa = 7.3VNFITIMCGILAGAMFNDD19 pKa = 4.15LGEE22 pKa = 4.58NPDD25 pKa = 3.17LTMGQRR31 pKa = 11.84YY32 pKa = 8.55FYY34 pKa = 10.2FAGFIVSMVIVLYY47 pKa = 10.77ALYY50 pKa = 10.16KK51 pKa = 10.64ASNEE55 pKa = 4.03MFGDD59 pKa = 4.69DD60 pKa = 5.89DD61 pKa = 5.55DD62 pKa = 4.54LTPAA66 pKa = 4.5

Molecular weight: 7.34 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A076YQC3|A0A076YQC3_9CAUD Uncharacterized protein OS=Rhizobium phage vB_RleM_P10VF OX=1527770 GN=P10VF_188 PE=4 SV=1
MM1 pKa = 7.2SGSRR5 pKa = 11.84GSARR9 pKa = 11.84KK10 pKa = 9.66DD11 pKa = 3.0RR12 pKa = 11.84VKK14 pKa = 10.43IRR16 pKa = 11.84VRR18 pKa = 11.84VWTDD22 pKa = 2.83LKK24 pKa = 11.29KK25 pKa = 10.42NSHH28 pKa = 5.12WTHH31 pKa = 5.95IFLPKK36 pKa = 9.89IHH38 pKa = 6.8NSDD41 pKa = 4.02MIHH44 pKa = 6.65EE45 pKa = 4.59KK46 pKa = 9.34LTKK49 pKa = 10.39KK50 pKa = 10.3FGSVAWYY57 pKa = 10.02DD58 pKa = 3.77PAPSRR63 pKa = 11.84AKK65 pKa = 10.52YY66 pKa = 10.34RR67 pKa = 11.84FVKK70 pKa = 10.62LL71 pKa = 3.44

Molecular weight: 8.41 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

257

0

257

48795

37

1716

189.9

21.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.357 ± 0.17

0.861 ± 0.055

7.046 ± 0.135

6.925 ± 0.206

5.076 ± 0.133

6.206 ± 0.223

1.875 ± 0.088

6.849 ± 0.141

6.892 ± 0.205

7.577 ± 0.144

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.515 ± 0.099

5.197 ± 0.118

3.48 ± 0.088

3.109 ± 0.096

5.113 ± 0.112

6.691 ± 0.189

6.152 ± 0.187

6.894 ± 0.159

1.244 ± 0.059

3.941 ± 0.131
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski