Amino acid dipepetide frequency for Brassica oleracea var. oleracea

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	5.854AlaAla: 5.854 ± 0.026	1.142AlaCys: 1.142 ± 0.008	2.9AlaAsp: 2.9 ± 0.013	4.16AlaGlu: 4.16 ± 0.019	2.675AlaPhe: 2.675 ± 0.011	3.745AlaGly: 3.745 ± 0.016	1.255AlaHis: 1.255 ± 0.009	3.268AlaIle: 3.268 ± 0.014	3.847AlaLys: 3.847 ± 0.015	5.99AlaLeu: 5.99 ± 0.019	1.73AlaMet: 1.73 ± 0.009	2.366AlaAsn: 2.366 ± 0.013	2.809AlaPro: 2.809 ± 0.014	1.929AlaGln: 1.929 ± 0.011	3.429AlaArg: 3.429 ± 0.015	5.872AlaSer: 5.872 ± 0.017	3.677AlaThr: 3.677 ± 0.015	4.69AlaVal: 4.69 ± 0.017	0.807AlaTrp: 0.807 ± 0.008	1.796AlaTyr: 1.796 ± 0.011	0.001AlaXaa: 0.001 ± 0.0
Cys	0.872CysAla: 0.872 ± 0.007	0.52CysCys: 0.52 ± 0.006	0.907CysAsp: 0.907 ± 0.007	0.919CysGlu: 0.919 ± 0.007	0.973CysPhe: 0.973 ± 0.007	1.419CysGly: 1.419 ± 0.01	0.44CysHis: 0.44 ± 0.004	0.931CysIle: 0.931 ± 0.007	1.134CysLys: 1.134 ± 0.009	1.896CysLeu: 1.896 ± 0.011	0.437CysMet: 0.437 ± 0.004	0.768CysAsn: 0.768 ± 0.006	0.926CysPro: 0.926 ± 0.01	0.526CysGln: 0.526 ± 0.005	1.087CysArg: 1.087 ± 0.008	1.757CysSer: 1.757 ± 0.01	0.852CysThr: 0.852 ± 0.006	1.211CysVal: 1.211 ± 0.009	0.241CysTrp: 0.241 ± 0.004	0.606CysTyr: 0.606 ± 0.006	0.0CysXaa: 0.0 ± 0.0
Asp	3.284AspAla: 3.284 ± 0.014	0.975AspCys: 0.975 ± 0.011	3.982AspAsp: 3.982 ± 0.023	4.354AspGlu: 4.354 ± 0.018	2.349AspPhe: 2.349 ± 0.011	3.762AspGly: 3.762 ± 0.015	1.37AspHis: 1.37 ± 0.01	2.874AspIle: 2.874 ± 0.013	2.845AspLys: 2.845 ± 0.013	5.248AspLeu: 5.248 ± 0.015	1.343AspMet: 1.343 ± 0.008	1.975AspAsn: 1.975 ± 0.01	2.689AspPro: 2.689 ± 0.012	1.838AspGln: 1.838 ± 0.01	2.539AspArg: 2.539 ± 0.014	4.231AspSer: 4.231 ± 0.016	2.365AspThr: 2.365 ± 0.012	3.896AspVal: 3.896 ± 0.016	0.666AspTrp: 0.666 ± 0.006	1.574AspTyr: 1.574 ± 0.009	0.0AspXaa: 0.0 ± 0.0
Glu	5.002GluAla: 5.002 ± 0.02	0.907GluCys: 0.907 ± 0.008	4.287GluAsp: 4.287 ± 0.02	7.093GluGlu: 7.093 ± 0.043	2.494GluPhe: 2.494 ± 0.011	3.546GluGly: 3.546 ± 0.017	1.286GluHis: 1.286 ± 0.009	3.753GluIle: 3.753 ± 0.013	4.933GluLys: 4.933 ± 0.022	5.692GluLeu: 5.692 ± 0.023	1.896GluMet: 1.896 ± 0.009	2.976GluAsn: 2.976 ± 0.013	2.269GluPro: 2.269 ± 0.013	2.033GluGln: 2.033 ± 0.012	3.748GluArg: 3.748 ± 0.017	4.764GluSer: 4.764 ± 0.021	3.714GluThr: 3.714 ± 0.019	4.278GluVal: 4.278 ± 0.016	0.773GluTrp: 0.773 ± 0.006	1.719GluTyr: 1.719 ± 0.012	0.001GluXaa: 0.001 ± 0.0
Phe	2.49PheAla: 2.49 ± 0.014	0.889PheCys: 0.889 ± 0.008	2.415PheAsp: 2.415 ± 0.012	2.354PheGlu: 2.354 ± 0.012	2.11PhePhe: 2.11 ± 0.013	3.092PheGly: 3.092 ± 0.014	1.069PheHis: 1.069 ± 0.009	2.041PheIle: 2.041 ± 0.011	2.235PheLys: 2.235 ± 0.014	4.252PheLeu: 4.252 ± 0.018	1.012PheMet: 1.012 ± 0.007	1.712PheAsn: 1.712 ± 0.008	2.133PhePro: 2.133 ± 0.012	1.489PheGln: 1.489 ± 0.009	2.256PheArg: 2.256 ± 0.014	4.091PheSer: 4.091 ± 0.016	2.267PheThr: 2.267 ± 0.011	2.903PheVal: 2.903 ± 0.015	0.664PheTrp: 0.664 ± 0.007	1.244PheTyr: 1.244 ± 0.008	0.0PheXaa: 0.0 ± 0.0
Gly	3.521GlyAla: 3.521 ± 0.018	1.267GlyCys: 1.267 ± 0.009	3.541GlyAsp: 3.541 ± 0.014	3.95GlyGlu: 3.95 ± 0.015	3.202GlyPhe: 3.202 ± 0.016	5.656GlyGly: 5.656 ± 0.055	1.442GlyHis: 1.442 ± 0.01	3.21GlyIle: 3.21 ± 0.017	4.108GlyLys: 4.108 ± 0.016	5.594GlyLeu: 5.594 ± 0.019	1.478GlyMet: 1.478 ± 0.009	2.87GlyAsn: 2.87 ± 0.014	2.288GlyPro: 2.288 ± 0.012	1.961GlyGln: 1.961 ± 0.011	3.695GlyArg: 3.695 ± 0.018	5.913GlySer: 5.913 ± 0.021	3.162GlyThr: 3.162 ± 0.015	4.26GlyVal: 4.26 ± 0.019	0.839GlyTrp: 0.839 ± 0.007	2.039GlyTyr: 2.039 ± 0.012	0.001GlyXaa: 0.001 ± 0.0
His	1.327HisAla: 1.327 ± 0.008	0.509HisCys: 0.509 ± 0.005	1.116HisAsp: 1.116 ± 0.008	1.284HisGlu: 1.284 ± 0.008	0.991HisPhe: 0.991 ± 0.007	1.655HisGly: 1.655 ± 0.01	0.997HisHis: 0.997 ± 0.01	1.159HisIle: 1.159 ± 0.009	1.208HisLys: 1.208 ± 0.007	2.327HisLeu: 2.327 ± 0.011	0.596HisMet: 0.596 ± 0.005	0.981HisAsn: 0.981 ± 0.007	1.32HisPro: 1.32 ± 0.009	1.053HisGln: 1.053 ± 0.009	1.434HisArg: 1.434 ± 0.009	1.835HisSer: 1.835 ± 0.011	0.984HisThr: 0.984 ± 0.008	1.606HisVal: 1.606 ± 0.01	0.343HisTrp: 0.343 ± 0.004	0.686HisTyr: 0.686 ± 0.007	0.0HisXaa: 0.0 ± 0.0
Ile	3.238IleAla: 3.238 ± 0.013	1.042IleCys: 1.042 ± 0.008	2.865IleAsp: 2.865 ± 0.014	3.007IleGlu: 3.007 ± 0.014	2.148IlePhe: 2.148 ± 0.015	3.206IleGly: 3.206 ± 0.013	1.249IleHis: 1.249 ± 0.007	2.653IleIle: 2.653 ± 0.013	2.871IleLys: 2.871 ± 0.013	4.706IleLeu: 4.706 ± 0.019	1.136IleMet: 1.136 ± 0.007	2.184IleAsn: 2.184 ± 0.01	2.837IlePro: 2.837 ± 0.018	1.835IleGln: 1.835 ± 0.01	2.656IleArg: 2.656 ± 0.013	4.608IleSer: 4.608 ± 0.015	2.645IleThr: 2.645 ± 0.013	3.444IleVal: 3.444 ± 0.014	0.712IleTrp: 0.712 ± 0.006	1.497IleTyr: 1.497 ± 0.011	0.0IleXaa: 0.0 ± 0.0
Lys	4.086LysAla: 4.086 ± 0.014	0.973LysCys: 0.973 ± 0.007	3.331LysAsp: 3.331 ± 0.016	4.791LysGlu: 4.791 ± 0.024	2.141LysPhe: 2.141 ± 0.011	3.57LysGly: 3.57 ± 0.015	1.345LysHis: 1.345 ± 0.011	3.274LysIle: 3.274 ± 0.013	5.414LysLys: 5.414 ± 0.026	5.903LysLeu: 5.903 ± 0.021	1.583LysMet: 1.583 ± 0.009	2.653LysAsn: 2.653 ± 0.014	2.944LysPro: 2.944 ± 0.016	2.229LysGln: 2.229 ± 0.012	4.016LysArg: 4.016 ± 0.016	4.632LysSer: 4.632 ± 0.021	3.411LysThr: 3.411 ± 0.013	3.764LysVal: 3.764 ± 0.014	0.885LysTrp: 0.885 ± 0.007	1.532LysTyr: 1.532 ± 0.009	0.0LysXaa: 0.0 ± 0.0
Leu	6.059LeuAla: 6.059 ± 0.02	1.855LeuCys: 1.855 ± 0.011	4.946LeuAsp: 4.946 ± 0.018	6.068LeuGlu: 6.068 ± 0.021	3.918LeuPhe: 3.918 ± 0.015	5.554LeuGly: 5.554 ± 0.018	2.395LeuHis: 2.395 ± 0.01	4.458LeuIle: 4.458 ± 0.016	5.936LeuLys: 5.936 ± 0.02	9.371LeuLeu: 9.371 ± 0.028	2.239LeuMet: 2.239 ± 0.011	3.719LeuAsn: 3.719 ± 0.017	4.952LeuPro: 4.952 ± 0.019	3.793LeuGln: 3.793 ± 0.017	5.606LeuArg: 5.606 ± 0.019	8.492LeuSer: 8.492 ± 0.024	4.656LeuThr: 4.656 ± 0.015	6.39LeuVal: 6.39 ± 0.017	1.256LeuTrp: 1.256 ± 0.008	2.353LeuTyr: 2.353 ± 0.012	0.001LeuXaa: 0.001 ± 0.0
Met	2.014MetAla: 2.014 ± 0.011	0.354MetCys: 0.354 ± 0.004	1.437MetAsp: 1.437 ± 0.009	2.001MetGlu: 2.001 ± 0.011	0.984MetPhe: 0.984 ± 0.007	1.515MetGly: 1.515 ± 0.009	0.523MetHis: 0.523 ± 0.005	1.323MetIle: 1.323 ± 0.009	1.763MetLys: 1.763 ± 0.009	2.096MetLeu: 2.096 ± 0.011	0.834MetMet: 0.834 ± 0.007	1.089MetAsn: 1.089 ± 0.007	0.954MetPro: 0.954 ± 0.008	0.784MetGln: 0.784 ± 0.007	1.37MetArg: 1.37 ± 0.008	1.991MetSer: 1.991 ± 0.011	1.128MetThr: 1.128 ± 0.007	1.782MetVal: 1.782 ± 0.009	0.292MetTrp: 0.292 ± 0.004	0.641MetTyr: 0.641 ± 0.005	0.0MetXaa: 0.0 ± 0.0
Asn	2.445AsnAla: 2.445 ± 0.012	0.776AsnCys: 0.776 ± 0.007	2.123AsnAsp: 2.123 ± 0.01	2.475AsnGlu: 2.475 ± 0.012	1.734AsnPhe: 1.734 ± 0.01	3.055AsnGly: 3.055 ± 0.016	1.134AsnHis: 1.134 ± 0.009	2.268AsnIle: 2.268 ± 0.013	2.455AsnLys: 2.455 ± 0.011	4.207AsnLeu: 4.207 ± 0.019	1.146AsnMet: 1.146 ± 0.009	2.319AsnAsn: 2.319 ± 0.018	2.375AsnPro: 2.375 ± 0.011	1.673AsnGln: 1.673 ± 0.01	2.14AsnArg: 2.14 ± 0.011	3.413AsnSer: 3.413 ± 0.013	2.039AsnThr: 2.039 ± 0.011	2.767AsnVal: 2.767 ± 0.013	0.538AsnTrp: 0.538 ± 0.006	1.185AsnTyr: 1.185 ± 0.008	0.0AsnXaa: 0.0 ± 0.0
Pro	2.914ProAla: 2.914 ± 0.015	0.781ProCys: 0.781 ± 0.008	2.489ProAsp: 2.489 ± 0.013	3.309ProGlu: 3.309 ± 0.014	1.968ProPhe: 1.968 ± 0.01	2.706ProGly: 2.706 ± 0.013	1.114ProHis: 1.114 ± 0.008	2.139ProIle: 2.139 ± 0.011	2.858ProLys: 2.858 ± 0.014	4.24ProLeu: 4.24 ± 0.016	1.022ProMet: 1.022 ± 0.008	2.114ProAsn: 2.114 ± 0.012	4.392ProPro: 4.392 ± 0.06	1.797ProGln: 1.797 ± 0.011	2.735ProArg: 2.735 ± 0.024	5.145ProSer: 5.145 ± 0.02	2.742ProThr: 2.742 ± 0.014	3.278ProVal: 3.278 ± 0.015	0.616ProTrp: 0.616 ± 0.01	1.352ProTyr: 1.352 ± 0.015	0.001ProXaa: 0.001 ± 0.0
Gln	2.235GlnAla: 2.235 ± 0.011	0.535GlnCys: 0.535 ± 0.005	1.627GlnAsp: 1.627 ± 0.008	2.448GlnGlu: 2.448 ± 0.014	1.286GlnPhe: 1.286 ± 0.007	2.067GlnGly: 2.067 ± 0.01	0.855GlnHis: 0.855 ± 0.007	1.829GlnIle: 1.829 ± 0.01	2.222GlnLys: 2.222 ± 0.011	3.156GlnLeu: 3.156 ± 0.013	0.935GlnMet: 0.935 ± 0.006	1.567GlnAsn: 1.567 ± 0.01	1.702GlnPro: 1.702 ± 0.012	1.956GlnGln: 1.956 ± 0.025	2.262GlnArg: 2.262 ± 0.014	2.734GlnSer: 2.734 ± 0.013	1.919GlnThr: 1.919 ± 0.009	2.221GlnVal: 2.221 ± 0.011	0.427GlnTrp: 0.427 ± 0.005	0.902GlnTyr: 0.902 ± 0.007	0.0GlnXaa: 0.0 ± 0.0
Arg	3.218ArgAla: 3.218 ± 0.015	1.109ArgCys: 1.109 ± 0.008	2.943ArgAsp: 2.943 ± 0.013	3.645ArgGlu: 3.645 ± 0.017	2.669ArgPhe: 2.669 ± 0.012	3.453ArgGly: 3.453 ± 0.018	1.274ArgHis: 1.274 ± 0.009	2.922ArgIle: 2.922 ± 0.012	3.99ArgLys: 3.99 ± 0.016	5.378ArgLeu: 5.378 ± 0.016	1.395ArgMet: 1.395 ± 0.008	2.525ArgAsn: 2.525 ± 0.017	2.447ArgPro: 2.447 ± 0.012	1.923ArgGln: 1.923 ± 0.01	4.604ArgArg: 4.604 ± 0.021	4.887ArgSer: 4.887 ± 0.02	2.607ArgThr: 2.607 ± 0.012	3.667ArgVal: 3.667 ± 0.016	0.789ArgTrp: 0.789 ± 0.006	1.51ArgTyr: 1.51 ± 0.01	0.001ArgXaa: 0.001 ± 0.0
Ser	4.963SerAla: 4.963 ± 0.019	1.686SerCys: 1.686 ± 0.01	4.568SerAsp: 4.568 ± 0.016	4.982SerGlu: 4.982 ± 0.023	4.076SerPhe: 4.076 ± 0.015	5.876SerGly: 5.876 ± 0.022	2.046SerHis: 2.046 ± 0.011	4.196SerIle: 4.196 ± 0.017	4.932SerLys: 4.932 ± 0.018	8.93SerLeu: 8.93 ± 0.027	2.015SerMet: 2.015 ± 0.01	3.685SerAsn: 3.685 ± 0.016	4.913SerPro: 4.913 ± 0.033	3.039SerGln: 3.039 ± 0.014	4.791SerArg: 4.791 ± 0.016	11.744SerSer: 11.744 ± 0.042	4.611SerThr: 4.611 ± 0.015	5.555SerVal: 5.555 ± 0.016	1.147SerTrp: 1.147 ± 0.009	2.438SerTyr: 2.438 ± 0.011	0.001SerXaa: 0.001 ± 0.0
Thr	3.375ThrAla: 3.375 ± 0.013	1.042ThrCys: 1.042 ± 0.009	2.492ThrAsp: 2.492 ± 0.012	3.121ThrGlu: 3.121 ± 0.015	2.121ThrPhe: 2.121 ± 0.011	3.368ThrGly: 3.368 ± 0.017	1.105ThrHis: 1.105 ± 0.007	2.714ThrIle: 2.714 ± 0.012	2.982ThrLys: 2.982 ± 0.013	4.73ThrLeu: 4.73 ± 0.017	1.272ThrMet: 1.272 ± 0.008	2.168ThrAsn: 2.168 ± 0.011	2.741ThrPro: 2.741 ± 0.014	1.688ThrGln: 1.688 ± 0.011	2.81ThrArg: 2.81 ± 0.013	4.938ThrSer: 4.938 ± 0.017	3.391ThrThr: 3.391 ± 0.015	3.629ThrVal: 3.629 ± 0.015	0.698ThrTrp: 0.698 ± 0.006	1.436ThrTyr: 1.436 ± 0.009	0.001ThrXaa: 0.001 ± 0.0
Val	4.701ValAla: 4.701 ± 0.015	1.209ValCys: 1.209 ± 0.008	3.877ValAsp: 3.877 ± 0.014	4.705ValGlu: 4.705 ± 0.018	2.91ValPhe: 2.91 ± 0.015	3.939ValGly: 3.939 ± 0.015	1.503ValHis: 1.503 ± 0.009	3.342ValIle: 3.342 ± 0.015	4.164ValLys: 4.164 ± 0.017	6.343ValLeu: 6.343 ± 0.018	1.725ValMet: 1.725 ± 0.008	2.657ValAsn: 2.657 ± 0.011	3.282ValPro: 3.282 ± 0.013	2.071ValGln: 2.071 ± 0.012	3.361ValArg: 3.361 ± 0.014	5.823ValSer: 5.823 ± 0.019	3.535ValThr: 3.535 ± 0.015	5.168ValVal: 5.168 ± 0.02	0.837ValTrp: 0.837 ± 0.006	1.99ValTyr: 1.99 ± 0.014	0.001ValXaa: 0.001 ± 0.0
Trp	0.683TrpAla: 0.683 ± 0.007	0.278TrpCys: 0.278 ± 0.003	0.76TrpAsp: 0.76 ± 0.006	0.839TrpGlu: 0.839 ± 0.007	0.64TrpPhe: 0.64 ± 0.006	0.703TrpGly: 0.703 ± 0.006	0.274TrpHis: 0.274 ± 0.004	0.752TrpIle: 0.752 ± 0.006	1.037TrpLys: 1.037 ± 0.008	1.224TrpLeu: 1.224 ± 0.009	0.32TrpMet: 0.32 ± 0.004	0.686TrpAsn: 0.686 ± 0.006	0.446TrpPro: 0.446 ± 0.005	0.386TrpGln: 0.386 ± 0.005	0.939TrpArg: 0.939 ± 0.008	1.101TrpSer: 1.101 ± 0.009	0.707TrpThr: 0.707 ± 0.007	0.802TrpVal: 0.802 ± 0.009	0.264TrpTrp: 0.264 ± 0.004	0.343TrpTyr: 0.343 ± 0.004	0.0TrpXaa: 0.0 ± 0.0
Tyr	1.661TyrAla: 1.661 ± 0.01	0.576TyrCys: 0.576 ± 0.005	1.552TyrAsp: 1.552 ± 0.011	1.658TyrGlu: 1.658 ± 0.009	1.329TyrPhe: 1.329 ± 0.008	2.109TyrGly: 2.109 ± 0.011	0.736TyrHis: 0.736 ± 0.007	1.431TyrIle: 1.431 ± 0.009	1.542TyrLys: 1.542 ± 0.012	2.731TyrLeu: 2.731 ± 0.013	0.763TyrMet: 0.763 ± 0.006	1.251TyrAsn: 1.251 ± 0.009	1.289TyrPro: 1.289 ± 0.009	0.884TyrGln: 0.884 ± 0.007	1.477TyrArg: 1.477 ± 0.009	2.224TyrSer: 2.224 ± 0.016	1.412TyrThr: 1.412 ± 0.01	1.832TyrVal: 1.832 ± 0.013	0.389TyrTrp: 0.389 ± 0.005	1.039TyrTyr: 1.039 ± 0.014	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.0	0.001XaaGlu: 0.001 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.001XaaLys: 0.001 ± 0.0	0.001XaaLeu: 0.001 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.001XaaPro: 0.001 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.001XaaArg: 0.001 ± 0.0	0.001XaaSer: 0.001 ± 0.0	0.001XaaThr: 0.001 ± 0.0	0.001XaaVal: 0.001 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.213XaaXaa: 0.213 ± 0.018

Statistics based on 58535 proteins (20370949 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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