Enterococcus sp. 9E7_DIV0242

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae; Enterococcus; unclassified Enterococcus

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4171 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A242K306|A0A242K306_9ENTE Uncharacterized protein OS=Enterococcus sp. 9E7_DIV0242 OX=1834193 GN=A5888_003352 PE=3 SV=1
MM1 pKa = 7.16TLAKK5 pKa = 9.51IVYY8 pKa = 10.46ASMTGNTEE16 pKa = 3.89EE17 pKa = 4.35IADD20 pKa = 3.64IVAEE24 pKa = 4.06AFEE27 pKa = 4.15NLNIEE32 pKa = 4.21VEE34 pKa = 4.04IDD36 pKa = 2.81EE37 pKa = 4.58CTQVDD42 pKa = 3.75AADD45 pKa = 4.33FAAADD50 pKa = 3.55ICVVATYY57 pKa = 9.24TYY59 pKa = 11.39GDD61 pKa = 3.83GEE63 pKa = 4.56LPDD66 pKa = 4.96EE67 pKa = 4.49IVDD70 pKa = 4.49FYY72 pKa = 11.82EE73 pKa = 4.8DD74 pKa = 3.65LQEE77 pKa = 4.15VDD79 pKa = 4.88LKK81 pKa = 11.23GKK83 pKa = 10.25IYY85 pKa = 9.85GVCGSGDD92 pKa = 3.4TFYY95 pKa = 11.53EE96 pKa = 4.99EE97 pKa = 4.43YY98 pKa = 10.16CKK100 pKa = 10.95SVDD103 pKa = 4.19DD104 pKa = 4.39FDD106 pKa = 5.96AVFTKK111 pKa = 10.41TGATKK116 pKa = 10.54GADD119 pKa = 3.25SVKK122 pKa = 10.04IDD124 pKa = 3.91LAAEE128 pKa = 4.1EE129 pKa = 4.69EE130 pKa = 4.74DD131 pKa = 3.73IQNLEE136 pKa = 3.97AFAKK140 pKa = 10.2KK141 pKa = 10.1LVEE144 pKa = 4.22AASS147 pKa = 3.45

Molecular weight: 15.94 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A242K416|A0A242K416_9ENTE Elongation factor P OS=Enterococcus sp. 9E7_DIV0242 OX=1834193 GN=efp PE=3 SV=1
MM1 pKa = 7.92PKK3 pKa = 10.12QKK5 pKa = 7.54THH7 pKa = 6.73RR8 pKa = 11.84GSAKK12 pKa = 9.62RR13 pKa = 11.84FKK15 pKa = 10.04RR16 pKa = 11.84TGKK19 pKa = 10.53GGLKK23 pKa = 9.97RR24 pKa = 11.84FRR26 pKa = 11.84AFTSHH31 pKa = 6.94RR32 pKa = 11.84FHH34 pKa = 7.48GKK36 pKa = 6.37TKK38 pKa = 9.57KK39 pKa = 9.43QRR41 pKa = 11.84RR42 pKa = 11.84QLRR45 pKa = 11.84KK46 pKa = 9.81ARR48 pKa = 11.84MVSAGDD54 pKa = 3.64FKK56 pKa = 11.18RR57 pKa = 11.84IRR59 pKa = 11.84QQLAGMKK66 pKa = 9.85

Molecular weight: 7.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4171

0

4171

1250518

19

4050

299.8

33.68

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.11 ± 0.034

0.726 ± 0.012

5.271 ± 0.034

7.674 ± 0.042

4.616 ± 0.028

6.622 ± 0.041

1.757 ± 0.018

7.473 ± 0.033

6.722 ± 0.032

9.881 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.021

4.474 ± 0.026

3.336 ± 0.022

4.075 ± 0.032

3.826 ± 0.026

6.234 ± 0.03

5.949 ± 0.034

6.783 ± 0.029

0.974 ± 0.013

3.806 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski