Elderberry carlavirus B

Taxonomy: Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Alsuviricetes; Tymovirales; Betaflexiviridae; Quinvirinae; Carlavirus; unclassified Carlavirus

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 6 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0A7M9B8|A0A0A7M9B8_9VIRU Movement protein TGB2 OS=Elderberry carlavirus B OX=1569053 PE=3 SV=1
MM1 pKa = 6.81WTLPNEE7 pKa = 4.07GLIAILIVTLICLIFSALNYY27 pKa = 8.16ITTDD31 pKa = 2.9KK32 pKa = 11.06GASCTVVISGEE43 pKa = 4.23SIVIRR48 pKa = 11.84GCEE51 pKa = 3.7FTEE54 pKa = 4.62QFIEE58 pKa = 4.25YY59 pKa = 10.51AKK61 pKa = 10.29GLRR64 pKa = 11.84VANHH68 pKa = 6.98KK69 pKa = 7.55LTPP72 pKa = 4.02

Molecular weight: 7.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0A7MDT2|A0A0A7MDT2_9VIRU RNA silencing suppressor OS=Elderberry carlavirus B OX=1569053 PE=3 SV=1
MM1 pKa = 7.78DD2 pKa = 4.39RR3 pKa = 11.84VKK5 pKa = 10.17TMCEE9 pKa = 3.76IISIMSSEE17 pKa = 4.44CRR19 pKa = 11.84DD20 pKa = 3.5SYY22 pKa = 11.34HH23 pKa = 7.86IDD25 pKa = 3.09IARR28 pKa = 11.84LIIKK32 pKa = 10.41LSDD35 pKa = 3.41KK36 pKa = 10.77DD37 pKa = 3.71RR38 pKa = 11.84QQGKK42 pKa = 9.96SSFAKK47 pKa = 9.8RR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84AKK52 pKa = 10.73ALDD55 pKa = 3.5RR56 pKa = 11.84CHH58 pKa = 6.41RR59 pKa = 11.84CYY61 pKa = 10.29RR62 pKa = 11.84VVPGFYY68 pKa = 9.07HH69 pKa = 4.89TTRR72 pKa = 11.84CNGIDD77 pKa = 3.56CTDD80 pKa = 3.2TFGTRR85 pKa = 11.84EE86 pKa = 3.88KK87 pKa = 10.79YY88 pKa = 10.25RR89 pKa = 11.84NQIVYY94 pKa = 8.75GQRR97 pKa = 11.84KK98 pKa = 5.07VTT100 pKa = 3.54

Molecular weight: 11.8 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

6

0

6

2804

72

1990

467.3

52.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.384 ± 0.561

3.531 ± 0.419

5.278 ± 0.492

6.419 ± 0.757

5.67 ± 0.701

6.348 ± 0.421

2.211 ± 0.3

5.956 ± 1.108

6.384 ± 0.739

9.344 ± 1.08

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.961 ± 0.402

4.529 ± 0.213

4.208 ± 0.539

2.318 ± 0.315

5.849 ± 0.689

7.703 ± 0.622

5.92 ± 0.617

5.813 ± 0.472

0.892 ± 0.168

3.281 ± 0.528
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski