Runella sp. YX9

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Cytophagia; Cytophagales; Cytophagaceae; Runella

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5856 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A369IJQ5|A0A369IJQ5_9BACT VCBS repeat-containing protein OS=Runella sp. YX9 OX=2282308 GN=DVG78_00665 PE=4 SV=1
AA1 pKa = 7.5TFLSPVWTVGTANGTVVATPTSVQPITTTTYY32 pKa = 11.26VLVAQNAEE40 pKa = 4.28GCQDD44 pKa = 3.35TSQVVVTVNAKK55 pKa = 9.95PNAGRR60 pKa = 11.84DD61 pKa = 3.51TTLACVDD68 pKa = 3.84ALSNTLATSYY78 pKa = 9.8TLVPIPPGGTWSQIGTTPTTATVTGNNVTGMTMAGTYY115 pKa = 8.79QFIYY119 pKa = 9.8TLNDD123 pKa = 3.11CSDD126 pKa = 3.94TVSVTVSPCQGCVQPNAGPDD146 pKa = 3.39QSICSPTTTATLTAITLGGTWTAQLGNPAPATITNLGAVSGMTIDD191 pKa = 3.29GTYY194 pKa = 10.52RR195 pKa = 11.84FIYY198 pKa = 10.19SVTSGGQTCTDD209 pKa = 3.38TVSVIRR215 pKa = 11.84NPKK218 pKa = 9.21PEE220 pKa = 4.83AGPDD224 pKa = 3.43QSICSPTTTATLTAITLGGTWSAQSGNPVAATITNAGTVTGMTANGTYY272 pKa = 10.83NFIYY276 pKa = 9.26MINGCTDD283 pKa = 3.15TVSVVRR289 pKa = 11.84TAKK292 pKa = 10.09PDD294 pKa = 3.32AGADD298 pKa = 3.53QTLTCPPSGISPTTANVTGAPSGGVWSTLASNPAAATVTNAGAVSGMTVAGTYY351 pKa = 8.82QFIYY355 pKa = 9.8TLNDD359 pKa = 3.09CSDD362 pKa = 3.63TVQIQVPTCIAPVFDD377 pKa = 4.39LALSKK382 pKa = 9.72TLAVGQSASVVAGSTVTFTITVFNQGNVDD411 pKa = 3.27ATNIQVTDD419 pKa = 4.2YY420 pKa = 10.84IPAGLTLNDD429 pKa = 4.37ANWSVVGSTATLNTVIASLAAGASTTRR456 pKa = 11.84DD457 pKa = 2.71ITFVVSTGVTGSITNLAEE475 pKa = 3.99ISSATGGVDD484 pKa = 3.16VDD486 pKa = 4.7SSPDD490 pKa = 3.51SNPANDD496 pKa = 4.15GTPKK500 pKa = 10.65NDD502 pKa = 3.55VINEE506 pKa = 3.96NGLTGGDD513 pKa = 4.08EE514 pKa = 4.62DD515 pKa = 4.7DD516 pKa = 5.1HH517 pKa = 8.83DD518 pKa = 4.81PEE520 pKa = 5.54VITVTPAPVFDD531 pKa = 3.84LALRR535 pKa = 11.84KK536 pKa = 7.82TLAVGQSASVVAGSTVTFTITVFNQGNVDD565 pKa = 3.27ATNIQVTDD573 pKa = 4.2YY574 pKa = 10.84IPAGLTLNDD583 pKa = 4.37ANWSVVGSTATLNTVIASLAAGASTTRR610 pKa = 11.84DD611 pKa = 2.71ITFVVSTGVTGSLSNLAEE629 pKa = 4.14ISSATGGVDD638 pKa = 3.16VDD640 pKa = 4.7SSPDD644 pKa = 3.53SNPADD649 pKa = 4.52DD650 pKa = 4.6GTPKK654 pKa = 10.64NDD656 pKa = 3.58VINEE660 pKa = 4.08SGLTGGDD667 pKa = 3.7EE668 pKa = 4.64DD669 pKa = 4.7DD670 pKa = 5.1HH671 pKa = 8.83DD672 pKa = 4.81PEE674 pKa = 5.47VITVTSAQVDD684 pKa = 4.11LSLKK688 pKa = 10.72KK689 pKa = 10.1MISTKK694 pKa = 9.92IVQLGDD700 pKa = 3.2TLTYY704 pKa = 8.81TIKK707 pKa = 10.63VFNQSGTLATGVEE720 pKa = 4.59VSDD723 pKa = 4.1SLATSVQFIMGSFTTTRR740 pKa = 11.84GTATLSNNVIVWTIGSIGANGDD762 pKa = 4.03TVTLTYY768 pKa = 9.96KK769 pKa = 10.61VKK771 pKa = 10.56AISEE775 pKa = 4.28GVHH778 pKa = 6.23FNTAEE783 pKa = 3.66ISKK786 pKa = 9.29TNEE789 pKa = 3.71PDD791 pKa = 3.16VDD793 pKa = 3.95STPGNGVGNEE803 pKa = 4.01DD804 pKa = 5.45DD805 pKa = 4.66IDD807 pKa = 3.99RR808 pKa = 11.84QCFTVPLKK816 pKa = 11.04LCVGEE821 pKa = 4.39KK822 pKa = 10.04IEE824 pKa = 4.19VSVPSYY830 pKa = 10.54LVNVQWYY837 pKa = 9.18KK838 pKa = 11.3DD839 pKa = 3.32GGTIPVVSGNVVLFSEE855 pKa = 4.6AGSYY859 pKa = 9.09TFTASNQQCPATGCCPIIIEE879 pKa = 4.97PGDD882 pKa = 3.46NCCPVDD888 pKa = 3.79LCIPFTIKK896 pKa = 8.34QTKK899 pKa = 9.41KK900 pKa = 10.36AGKK903 pKa = 8.71PLL905 pKa = 3.66

Molecular weight: 92.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A369IIA9|A0A369IIA9_9BACT Uncharacterized protein OS=Runella sp. YX9 OX=2282308 GN=DVG78_06885 PE=4 SV=1
MM1 pKa = 6.98KK2 pKa = 10.28QVIAYY7 pKa = 9.0YY8 pKa = 10.02RR9 pKa = 11.84VSTQRR14 pKa = 11.84QGRR17 pKa = 11.84SGLGLEE23 pKa = 4.33AQQNAVLNYY32 pKa = 9.18CKK34 pKa = 10.53LSGYY38 pKa = 8.11EE39 pKa = 3.93VIRR42 pKa = 11.84EE43 pKa = 4.06VTEE46 pKa = 4.13VKK48 pKa = 9.24STRR51 pKa = 11.84KK52 pKa = 10.02DD53 pKa = 3.39RR54 pKa = 11.84IGLLQALDD62 pKa = 3.98LCRR65 pKa = 11.84QMKK68 pKa = 10.29AILMVARR75 pKa = 11.84LDD77 pKa = 3.59RR78 pKa = 11.84LGRR81 pKa = 11.84DD82 pKa = 3.5VEE84 pKa = 4.41QIAGIVKK91 pKa = 10.1SKK93 pKa = 11.26VEE95 pKa = 4.63IIVTDD100 pKa = 3.57NPHH103 pKa = 6.55ANRR106 pKa = 11.84FTIHH110 pKa = 6.35ILAAVAEE117 pKa = 4.41DD118 pKa = 3.18NRR120 pKa = 11.84RR121 pKa = 11.84RR122 pKa = 11.84ISEE125 pKa = 4.23TTKK128 pKa = 10.84DD129 pKa = 3.37ALGAAKK135 pKa = 10.15RR136 pKa = 11.84RR137 pKa = 11.84GVILGKK143 pKa = 10.36NGTALSIANKK153 pKa = 9.73KK154 pKa = 9.32AAEE157 pKa = 4.42DD158 pKa = 3.85FAQQLSPTLKK168 pKa = 9.69RR169 pKa = 11.84LKK171 pKa = 9.34TRR173 pKa = 11.84GIISVRR179 pKa = 11.84AVCRR183 pKa = 11.84EE184 pKa = 3.84LNKK187 pKa = 10.44RR188 pKa = 11.84QIPTFRR194 pKa = 11.84GDD196 pKa = 4.0GRR198 pKa = 11.84WHH200 pKa = 6.95PSTVNVLMNRR210 pKa = 11.84LKK212 pKa = 9.68QTIISPP218 pKa = 4.03

Molecular weight: 24.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5856

0

5856

2137984

26

4355

365.1

40.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.56 ± 0.03

0.805 ± 0.01

5.059 ± 0.031

5.843 ± 0.033

5.002 ± 0.024

7.006 ± 0.038

1.861 ± 0.016

6.471 ± 0.024

6.428 ± 0.036

9.663 ± 0.038

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.23 ± 0.015

5.238 ± 0.032

4.193 ± 0.019

4.034 ± 0.019

4.412 ± 0.025

6.147 ± 0.024

6.111 ± 0.043

6.634 ± 0.025

1.368 ± 0.014

3.934 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski