Lynx canadensis faeces associated genomovirus CL5 48
Average proteome isoelectric point is 6.99
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CJ25|A0A2Z5CJ25_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL5 48 OX=2219132 PE=4 SV=1
MM1 pKa = 8.1 PSFLCNARR9 pKa = 11.84 HH10 pKa = 6.08 FLITYY15 pKa = 7.25 AQCGDD20 pKa = 3.49 LSEE23 pKa = 4.24 WDD25 pKa = 3.47 VLDD28 pKa = 4.18 RR29 pKa = 11.84 FSEE32 pKa = 4.18 LGAEE36 pKa = 4.36 CIIARR41 pKa = 11.84 EE42 pKa = 3.95 YY43 pKa = 10.86 HH44 pKa = 6.71 ADD46 pKa = 3.65 LGLHH50 pKa = 5.8 LHH52 pKa = 5.84 VFVDD56 pKa = 4.55 FGRR59 pKa = 11.84 KK60 pKa = 7.9 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 VDD67 pKa = 2.87 IFDD70 pKa = 3.35 VAGRR74 pKa = 11.84 HH75 pKa = 5.17 PNIKK79 pKa = 9.65 RR80 pKa = 11.84 SYY82 pKa = 7.64 GTPEE86 pKa = 3.49 KK87 pKa = 10.79 GYY89 pKa = 10.83 DD90 pKa = 3.43 YY91 pKa = 10.61 AIKK94 pKa = 10.61 DD95 pKa = 3.82 GEE97 pKa = 4.57 VVAGGLEE104 pKa = 4.19 RR105 pKa = 11.84 PDD107 pKa = 3.36 GTFGSGKK114 pKa = 10.5 SFDD117 pKa = 3.78 LWSQITGAGDD127 pKa = 3.63 RR128 pKa = 11.84 EE129 pKa = 4.5 EE130 pKa = 4.01 FWKK133 pKa = 10.86 LLEE136 pKa = 4.26 EE137 pKa = 4.97 LDD139 pKa = 4.44 PKK141 pKa = 10.69 SLCCSFTQLQKK152 pKa = 9.72 YY153 pKa = 9.81 ADD155 pKa = 3.24 WRR157 pKa = 11.84 FAEE160 pKa = 4.5 RR161 pKa = 11.84 PAVYY165 pKa = 9.52 EE166 pKa = 4.15 SPGGFEE172 pKa = 4.28 FVSGDD177 pKa = 2.87 VDD179 pKa = 4.96 GRR181 pKa = 11.84 DD182 pKa = 3.45 QPLSLVLYY190 pKa = 10.24 GEE192 pKa = 4.27 SRR194 pKa = 11.84 TGKK197 pKa = 7.35 TLWARR202 pKa = 11.84 SLGPHH207 pKa = 7.22 IYY209 pKa = 10.46 NVGLVSGEE217 pKa = 4.08 EE218 pKa = 4.12 CMKK221 pKa = 10.78 ASSVEE226 pKa = 3.76 YY227 pKa = 10.54 AIFDD231 pKa = 4.95 DD232 pKa = 3.45 IRR234 pKa = 11.84 GGIKK238 pKa = 9.97 FFPAFKK244 pKa = 9.81 EE245 pKa = 4.05 WLGGQQTVCVKK256 pKa = 10.44 RR257 pKa = 11.84 LYY259 pKa = 10.36 RR260 pKa = 11.84 DD261 pKa = 3.27 PKK263 pKa = 9.83 LIQWGKK269 pKa = 7.93 PSIWISNDD277 pKa = 3.18 DD278 pKa = 3.94 PRR280 pKa = 11.84 HH281 pKa = 5.68 SMEE284 pKa = 4.21 TSDD287 pKa = 4.84 VAWLEE292 pKa = 4.27 MNCKK296 pKa = 9.48 FVEE299 pKa = 3.98 INTPIFRR306 pKa = 11.84 ANTEE310 pKa = 3.84
Molecular weight: 35.26 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.202
IPC2_protein 5.27
IPC_protein 5.232
Toseland 5.258
ProMoST 5.321
Dawson 5.283
Bjellqvist 5.397
Wikipedia 5.181
Rodwell 5.207
Grimsley 5.219
Solomon 5.283
Lehninger 5.245
Nozaki 5.423
DTASelect 5.588
Thurlkill 5.308
EMBOSS 5.27
Sillero 5.499
Patrickios 4.037
IPC_peptide 5.283
IPC2_peptide 5.499
IPC2.peptide.svr19 5.448
Protein with the highest isoelectric point:
>tr|A0A2Z5CJ25|A0A2Z5CJ25_9VIRU Capsid protein OS=Lynx canadensis faeces associated genomovirus CL5 48 OX=2219132 PE=4 SV=1
MM1 pKa = 7.91 AYY3 pKa = 9.72 RR4 pKa = 11.84 RR5 pKa = 11.84 KK6 pKa = 10.24 SGSYY10 pKa = 7.85 KK11 pKa = 9.99 AKK13 pKa = 9.9 RR14 pKa = 11.84 RR15 pKa = 11.84 TRR17 pKa = 11.84 RR18 pKa = 11.84 SARR21 pKa = 11.84 PSRR24 pKa = 11.84 STGRR28 pKa = 11.84 TRR30 pKa = 11.84 PYY32 pKa = 10.44 RR33 pKa = 11.84 RR34 pKa = 11.84 TKK36 pKa = 10.34 RR37 pKa = 11.84 ISRR40 pKa = 11.84 PMSKK44 pKa = 10.11 KK45 pKa = 10.18 RR46 pKa = 11.84 ILNVTSTKK54 pKa = 10.38 KK55 pKa = 10.21 RR56 pKa = 11.84 DD57 pKa = 3.69 DD58 pKa = 3.46 MSSYY62 pKa = 10.99 YY63 pKa = 10.4 YY64 pKa = 10.0 QANSAGTGYY73 pKa = 11.07 NSGFSASVAAGNQTGASSANPRR95 pKa = 11.84 TTGYY99 pKa = 9.1 IWCPTARR106 pKa = 11.84 DD107 pKa = 4.26 FTSSTAGGKK116 pKa = 10.78 GNVSDD121 pKa = 3.75 QATRR125 pKa = 11.84 TASTCYY131 pKa = 9.18 MRR133 pKa = 11.84 GIAEE137 pKa = 4.56 RR138 pKa = 11.84 IEE140 pKa = 4.16 IQTSTGIPWQWRR152 pKa = 11.84 RR153 pKa = 11.84 ICFKK157 pKa = 10.93 YY158 pKa = 10.29 RR159 pKa = 11.84 GNLYY163 pKa = 10.42 GGNGNSQAGAYY174 pKa = 6.32 QTHH177 pKa = 6.93 IEE179 pKa = 4.3 TSNGFQRR186 pKa = 11.84 VSVQFTDD193 pKa = 3.57 TTATGNSIEE202 pKa = 4.15 NSLTNLIFRR211 pKa = 11.84 GTPGSGDD218 pKa = 2.59 WFDD221 pKa = 4.32 YY222 pKa = 10.64 MNAPLDD228 pKa = 3.51 PTRR231 pKa = 11.84 IDD233 pKa = 2.7 ICYY236 pKa = 10.3 DD237 pKa = 3.08 RR238 pKa = 11.84 QTVVSSGNASGKK250 pKa = 9.93 IFAKK254 pKa = 10.58 KK255 pKa = 9.65 LWHH258 pKa = 6.58 PMNKK262 pKa = 9.58 NLVYY266 pKa = 10.81 DD267 pKa = 4.53 DD268 pKa = 5.22 DD269 pKa = 4.4 EE270 pKa = 5.12 QGGTEE275 pKa = 3.74 SSTYY279 pKa = 10.23 YY280 pKa = 10.0 SQHH283 pKa = 5.68 GKK285 pKa = 9.84 QGMGDD290 pKa = 3.66 YY291 pKa = 10.84 YY292 pKa = 10.49 IVDD295 pKa = 4.52 FFKK298 pKa = 11.07 AGIGAAQADD307 pKa = 3.89 QLSFEE312 pKa = 4.57 PQSTLYY318 pKa = 9.41 WHH320 pKa = 7.02 EE321 pKa = 4.09 KK322 pKa = 9.32
Molecular weight: 35.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.783
IPC2_protein 9.531
IPC_protein 9.78
Toseland 9.838
ProMoST 9.736
Dawson 10.145
Bjellqvist 9.897
Wikipedia 10.365
Rodwell 10.35
Grimsley 10.248
Solomon 10.16
Lehninger 10.116
Nozaki 9.882
DTASelect 9.882
Thurlkill 9.955
EMBOSS 10.277
Sillero 10.058
Patrickios 5.067
IPC_peptide 10.16
IPC2_peptide 8.77
IPC2.peptide.svr19 8.474
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
632
310
322
316.0
35.57
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.804 ± 0.649
1.899 ± 0.444
6.171 ± 0.813
4.905 ± 1.637
4.905 ± 1.007
9.652 ± 0.193
1.741 ± 0.337
5.063 ± 0.064
5.222 ± 0.039
5.38 ± 1.748
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.741 ± 0.293
3.797 ± 1.002
3.956 ± 0.365
3.797 ± 0.792
7.753 ± 0.637
9.019 ± 1.251
6.646 ± 2.016
4.272 ± 0.999
2.373 ± 0.345
4.905 ± 0.883
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here