Rickettsiales bacterium
Average proteome isoelectric point is 6.97
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2001 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1SY14|A0A4V1SY14_9RICK ParA family protein (Fragment) OS=Rickettsiales bacterium OX=2026788 GN=EOP34_09365 PE=4 SV=1
MM1 pKa = 7.75 SINSLVQYY9 pKa = 9.32 FSILTVSFHH18 pKa = 7.76 DD19 pKa = 3.58 INSLYY24 pKa = 10.85 GFFLILVVVSQLVSGTMVSFSLIPEE49 pKa = 4.05 PMIVPIVRR57 pKa = 11.84 DD58 pKa = 3.77 EE59 pKa = 4.28 EE60 pKa = 4.32 DD61 pKa = 5.13 LEE63 pKa = 5.12 DD64 pKa = 5.82 LYY66 pKa = 11.27 TDD68 pKa = 4.6 DD69 pKa = 5.98 FFF71 pKa = 7.11
Molecular weight: 8.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.845
IPC2_protein 3.617
IPC_protein 3.49
Toseland 3.312
ProMoST 3.719
Dawson 3.516
Bjellqvist 3.694
Wikipedia 3.503
Rodwell 3.35
Grimsley 3.249
Solomon 3.452
Lehninger 3.414
Nozaki 3.681
DTASelect 3.834
Thurlkill 3.414
EMBOSS 3.503
Sillero 3.63
Patrickios 0.477
IPC_peptide 3.452
IPC2_peptide 3.592
IPC2.peptide.svr19 3.676
Protein with the highest isoelectric point:
>tr|A0A4Q3E1L0|A0A4Q3E1L0_9RICK 50S ribosomal protein L19 (Fragment) OS=Rickettsiales bacterium OX=2026788 GN=EOP34_10145 PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.27 QPSKK9 pKa = 9.73 LVRR12 pKa = 11.84 KK13 pKa = 8.95 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.68 GFRR19 pKa = 11.84 SRR21 pKa = 11.84 MSTVGGRR28 pKa = 11.84 KK29 pKa = 8.66 VLNRR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 KK40 pKa = 9.25 KK41 pKa = 10.65 LSAA44 pKa = 3.91
Molecular weight: 5.24 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2001
0
2001
353358
15
1792
176.6
20.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.184 ± 0.062
1.124 ± 0.027
4.645 ± 0.059
4.572 ± 0.061
5.92 ± 0.065
5.326 ± 0.056
1.903 ± 0.032
10.254 ± 0.08
6.593 ± 0.073
11.209 ± 0.094
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.353 ± 0.028
6.848 ± 0.069
3.098 ± 0.048
2.688 ± 0.039
3.26 ± 0.045
8.206 ± 0.063
5.478 ± 0.054
5.732 ± 0.06
0.706 ± 0.022
4.902 ± 0.05
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here