Amino acid dipepetide frequency for Eubacterium siraeum CAG:80

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	6.972AlaAla: 6.972 ± 0.119	1.189AlaCys: 1.189 ± 0.04	5.627AlaAsp: 5.627 ± 0.078	5.914AlaGlu: 5.914 ± 0.096	3.088AlaPhe: 3.088 ± 0.082	5.425AlaGly: 5.425 ± 0.101	1.058AlaHis: 1.058 ± 0.04	5.184AlaIle: 5.184 ± 0.106	5.617AlaLys: 5.617 ± 0.11	6.823AlaLeu: 6.823 ± 0.12	2.306AlaMet: 2.306 ± 0.056	2.945AlaAsn: 2.945 ± 0.065	2.222AlaPro: 2.222 ± 0.061	2.421AlaGln: 2.421 ± 0.061	2.666AlaArg: 2.666 ± 0.066	3.996AlaSer: 3.996 ± 0.086	3.764AlaThr: 3.764 ± 0.085	7.537AlaVal: 7.537 ± 0.121	0.526AlaTrp: 0.526 ± 0.029	2.826AlaTyr: 2.826 ± 0.071	0.0AlaXaa: 0.0 ± 0.0
Cys	1.378CysAla: 1.378 ± 0.052	0.378CysCys: 0.378 ± 0.024	1.28CysAsp: 1.28 ± 0.045	1.252CysGlu: 1.252 ± 0.04	0.771CysPhe: 0.771 ± 0.034	1.823CysGly: 1.823 ± 0.049	0.29CysHis: 0.29 ± 0.021	1.257CysIle: 1.257 ± 0.037	0.948CysLys: 0.948 ± 0.034	1.12CysLeu: 1.12 ± 0.042	0.373CysMet: 0.373 ± 0.02	0.717CysAsn: 0.717 ± 0.034	0.652CysPro: 0.652 ± 0.032	0.24CysGln: 0.24 ± 0.02	0.822CysArg: 0.822 ± 0.035	1.218CysSer: 1.218 ± 0.041	0.973CysThr: 0.973 ± 0.039	1.131CysVal: 1.131 ± 0.038	0.117CysTrp: 0.117 ± 0.013	0.724CysTyr: 0.724 ± 0.033	0.0CysXaa: 0.0 ± 0.0
Asp	4.027AspAla: 4.027 ± 0.078	1.039AspCys: 1.039 ± 0.042	3.949AspAsp: 3.949 ± 0.082	5.179AspGlu: 5.179 ± 0.096	2.945AspPhe: 2.945 ± 0.071	5.226AspGly: 5.226 ± 0.099	0.617AspHis: 0.617 ± 0.032	5.61AspIle: 5.61 ± 0.083	5.292AspLys: 5.292 ± 0.094	3.87AspLeu: 3.87 ± 0.078	2.033AspMet: 2.033 ± 0.054	3.448AspAsn: 3.448 ± 0.075	1.672AspPro: 1.672 ± 0.051	0.637AspGln: 0.637 ± 0.033	2.534AspArg: 2.534 ± 0.066	3.925AspSer: 3.925 ± 0.082	3.734AspThr: 3.734 ± 0.083	3.924AspVal: 3.924 ± 0.078	0.467AspTrp: 0.467 ± 0.028	3.04AspTyr: 3.04 ± 0.073	0.0AspXaa: 0.0 ± 0.0
Glu	4.131GluAla: 4.131 ± 0.09	0.938GluCys: 0.938 ± 0.032	3.359GluAsp: 3.359 ± 0.074	4.69GluGlu: 4.69 ± 0.1	2.461GluPhe: 2.461 ± 0.059	3.408GluGly: 3.408 ± 0.07	1.186GluHis: 1.186 ± 0.041	5.571GluIle: 5.571 ± 0.091	6.57GluLys: 6.57 ± 0.106	6.224GluLeu: 6.224 ± 0.113	2.143GluMet: 2.143 ± 0.061	4.615GluAsn: 4.615 ± 0.087	1.758GluPro: 1.758 ± 0.048	2.732GluGln: 2.732 ± 0.07	2.867GluArg: 2.867 ± 0.072	3.419GluSer: 3.419 ± 0.066	3.544GluThr: 3.544 ± 0.074	3.572GluVal: 3.572 ± 0.069	0.512GluTrp: 0.512 ± 0.037	3.246GluTyr: 3.246 ± 0.084	0.0GluXaa: 0.0 ± 0.0
Phe	3.371PheAla: 3.371 ± 0.072	0.803PheCys: 0.803 ± 0.036	3.179PheAsp: 3.179 ± 0.071	2.52PheGlu: 2.52 ± 0.066	1.764PhePhe: 1.764 ± 0.063	3.114PheGly: 3.114 ± 0.063	0.475PheHis: 0.475 ± 0.028	3.202PheIle: 3.202 ± 0.077	2.242PheLys: 2.242 ± 0.054	2.946PheLeu: 2.946 ± 0.078	1.12PheMet: 1.12 ± 0.041	1.729PheAsn: 1.729 ± 0.047	1.263PhePro: 1.263 ± 0.043	0.76PheGln: 0.76 ± 0.03	1.728PheArg: 1.728 ± 0.042	3.295PheSer: 3.295 ± 0.072	2.949PheThr: 2.949 ± 0.068	2.75PheVal: 2.75 ± 0.064	0.295PheTrp: 0.295 ± 0.023	1.725PheTyr: 1.725 ± 0.045	0.0PheXaa: 0.0 ± 0.0
Gly	4.833GlyAla: 4.833 ± 0.102	1.374GlyCys: 1.374 ± 0.041	3.993GlyAsp: 3.993 ± 0.075	4.749GlyGlu: 4.749 ± 0.083	3.236GlyPhe: 3.236 ± 0.068	5.115GlyGly: 5.115 ± 0.108	0.997GlyHis: 0.997 ± 0.037	5.876GlyIle: 5.876 ± 0.093	5.707GlyLys: 5.707 ± 0.095	5.33GlyLeu: 5.33 ± 0.099	2.112GlyMet: 2.112 ± 0.053	3.454GlyAsn: 3.454 ± 0.078	0.894GlyPro: 0.894 ± 0.04	1.582GlyGln: 1.582 ± 0.05	2.855GlyArg: 2.855 ± 0.064	4.214GlySer: 4.214 ± 0.084	4.513GlyThr: 4.513 ± 0.087	4.915GlyVal: 4.915 ± 0.096	0.545GlyTrp: 0.545 ± 0.034	3.314GlyTyr: 3.314 ± 0.067	0.004GlyXaa: 0.004 ± 0.002
His	0.847HisAla: 0.847 ± 0.039	0.349HisCys: 0.349 ± 0.023	0.829HisAsp: 0.829 ± 0.035	0.926HisGlu: 0.926 ± 0.04	0.716HisPhe: 0.716 ± 0.032	1.216HisGly: 1.216 ± 0.041	0.285HisHis: 0.285 ± 0.02	1.315HisIle: 1.315 ± 0.045	0.96HisLys: 0.96 ± 0.032	1.115HisLeu: 1.115 ± 0.04	0.286HisMet: 0.286 ± 0.021	0.805HisAsn: 0.805 ± 0.032	0.671HisPro: 0.671 ± 0.034	0.313HisGln: 0.313 ± 0.02	0.656HisArg: 0.656 ± 0.032	1.051HisSer: 1.051 ± 0.041	0.935HisThr: 0.935 ± 0.037	0.649HisVal: 0.649 ± 0.035	0.136HisTrp: 0.136 ± 0.013	0.693HisTyr: 0.693 ± 0.033	0.0HisXaa: 0.0 ± 0.0
Ile	6.337IleAla: 6.337 ± 0.102	1.458IleCys: 1.458 ± 0.046	4.743IleAsp: 4.743 ± 0.083	4.61IleGlu: 4.61 ± 0.091	2.913IlePhe: 2.913 ± 0.073	5.026IleGly: 5.026 ± 0.097	1.044IleHis: 1.044 ± 0.042	5.981IleIle: 5.981 ± 0.124	5.256IleLys: 5.256 ± 0.089	5.789IleLeu: 5.789 ± 0.109	2.087IleMet: 2.087 ± 0.05	3.699IleAsn: 3.699 ± 0.083	3.026IlePro: 3.026 ± 0.074	1.496IleGln: 1.496 ± 0.048	3.195IleArg: 3.195 ± 0.077	5.86IleSer: 5.86 ± 0.094	5.226IleThr: 5.226 ± 0.082	4.928IleVal: 4.928 ± 0.088	0.481IleTrp: 0.481 ± 0.028	2.791IleTyr: 2.791 ± 0.068	0.0IleXaa: 0.0 ± 0.0
Lys	6.106LysAla: 6.106 ± 0.101	0.98LysCys: 0.98 ± 0.035	3.993LysAsp: 3.993 ± 0.083	5.073LysGlu: 5.073 ± 0.1	2.158LysPhe: 2.158 ± 0.057	4.261LysGly: 4.261 ± 0.08	1.05LysHis: 1.05 ± 0.044	5.021LysIle: 5.021 ± 0.082	5.757LysLys: 5.757 ± 0.095	6.301LysLeu: 6.301 ± 0.086	2.094LysMet: 2.094 ± 0.054	4.17LysAsn: 4.17 ± 0.079	2.505LysPro: 2.505 ± 0.074	2.482LysGln: 2.482 ± 0.063	3.077LysArg: 3.077 ± 0.064	4.37LysSer: 4.37 ± 0.084	4.415LysThr: 4.415 ± 0.085	4.29LysVal: 4.29 ± 0.075	0.624LysTrp: 0.624 ± 0.033	3.074LysTyr: 3.074 ± 0.068	0.0LysXaa: 0.0 ± 0.0
Leu	6.254LeuAla: 6.254 ± 0.1	1.811LeuCys: 1.811 ± 0.057	5.032LeuAsp: 5.032 ± 0.094	4.781LeuGlu: 4.781 ± 0.092	3.496LeuPhe: 3.496 ± 0.087	5.467LeuGly: 5.467 ± 0.093	1.248LeuHis: 1.248 ± 0.046	6.188LeuIle: 6.188 ± 0.105	5.657LeuLys: 5.657 ± 0.094	6.973LeuLeu: 6.973 ± 0.126	2.338LeuMet: 2.338 ± 0.066	3.39LeuAsn: 3.39 ± 0.058	3.278LeuPro: 3.278 ± 0.061	1.926LeuGln: 1.926 ± 0.057	3.425LeuArg: 3.425 ± 0.073	6.928LeuSer: 6.928 ± 0.123	5.267LeuThr: 5.267 ± 0.101	4.8LeuVal: 4.8 ± 0.11	0.618LeuTrp: 0.618 ± 0.033	3.236LeuTyr: 3.236 ± 0.072	0.0LeuXaa: 0.0 ± 0.0
Met	2.225MetAla: 2.225 ± 0.06	0.444MetCys: 0.444 ± 0.025	1.359MetAsp: 1.359 ± 0.04	1.465MetGlu: 1.465 ± 0.043	1.058MetPhe: 1.058 ± 0.044	1.758MetGly: 1.758 ± 0.054	0.455MetHis: 0.455 ± 0.028	2.004MetIle: 2.004 ± 0.053	2.25MetLys: 2.25 ± 0.056	2.761MetLeu: 2.761 ± 0.066	0.818MetMet: 0.818 ± 0.033	1.585MetAsn: 1.585 ± 0.045	1.233MetPro: 1.233 ± 0.041	0.904MetGln: 0.904 ± 0.039	1.167MetArg: 1.167 ± 0.036	2.019MetSer: 2.019 ± 0.054	1.887MetThr: 1.887 ± 0.054	1.53MetVal: 1.53 ± 0.05	0.201MetTrp: 0.201 ± 0.015	1.029MetTyr: 1.029 ± 0.041	0.0MetXaa: 0.0 ± 0.0
Asn	3.956AsnAla: 3.956 ± 0.086	0.856AsnCys: 0.856 ± 0.034	2.956AsnAsp: 2.956 ± 0.057	3.327AsnGlu: 3.327 ± 0.068	1.711AsnPhe: 1.711 ± 0.055	4.425AsnGly: 4.425 ± 0.083	0.675AsnHis: 0.675 ± 0.03	4.03AsnIle: 4.03 ± 0.081	3.146AsnLys: 3.146 ± 0.06	3.327AsnLeu: 3.327 ± 0.076	1.266AsnMet: 1.266 ± 0.04	2.5AsnAsn: 2.5 ± 0.072	1.82AsnPro: 1.82 ± 0.052	0.875AsnGln: 0.875 ± 0.036	1.861AsnArg: 1.861 ± 0.05	3.157AsnSer: 3.157 ± 0.09	2.923AsnThr: 2.923 ± 0.07	3.311AsnVal: 3.311 ± 0.071	0.396AsnTrp: 0.396 ± 0.027	1.92AsnTyr: 1.92 ± 0.054	0.0AsnXaa: 0.0 ± 0.0
Pro	2.677ProAla: 2.677 ± 0.066	0.553ProCys: 0.553 ± 0.028	2.738ProAsp: 2.738 ± 0.071	2.852ProGlu: 2.852 ± 0.067	1.513ProPhe: 1.513 ± 0.051	1.668ProGly: 1.668 ± 0.049	0.584ProHis: 0.584 ± 0.027	1.876ProIle: 1.876 ± 0.05	1.954ProLys: 1.954 ± 0.054	2.585ProLeu: 2.585 ± 0.06	0.828ProMet: 0.828 ± 0.034	1.116ProAsn: 1.116 ± 0.042	0.934ProPro: 0.934 ± 0.045	1.105ProGln: 1.105 ± 0.043	0.888ProArg: 0.888 ± 0.032	1.877ProSer: 1.877 ± 0.051	1.737ProThr: 1.737 ± 0.052	2.991ProVal: 2.991 ± 0.068	0.242ProTrp: 0.242 ± 0.023	1.357ProTyr: 1.357 ± 0.045	0.0ProXaa: 0.0 ± 0.0
Gln	1.924GlnAla: 1.924 ± 0.056	0.423GlnCys: 0.423 ± 0.023	1.119GlnAsp: 1.119 ± 0.038	1.42GlnGlu: 1.42 ± 0.039	1.071GlnPhe: 1.071 ± 0.037	1.483GlnGly: 1.483 ± 0.041	0.453GlnHis: 0.453 ± 0.024	2.076GlnIle: 2.076 ± 0.056	1.913GlnLys: 1.913 ± 0.057	2.593GlnLeu: 2.593 ± 0.061	0.81GlnMet: 0.81 ± 0.031	1.28GlnAsn: 1.28 ± 0.048	0.817GlnPro: 0.817 ± 0.039	0.967GlnGln: 0.967 ± 0.041	1.255GlnArg: 1.255 ± 0.047	1.583GlnSer: 1.583 ± 0.048	1.612GlnThr: 1.612 ± 0.049	1.397GlnVal: 1.397 ± 0.046	0.271GlnTrp: 0.271 ± 0.02	1.247GlnTyr: 1.247 ± 0.043	0.0GlnXaa: 0.0 ± 0.0
Arg	2.572ArgAla: 2.572 ± 0.061	0.738ArgCys: 0.738 ± 0.034	2.094ArgAsp: 2.094 ± 0.049	2.724ArgGlu: 2.724 ± 0.06	1.959ArgPhe: 1.959 ± 0.049	1.929ArgGly: 1.929 ± 0.056	0.739ArgHis: 0.739 ± 0.034	3.559ArgIle: 3.559 ± 0.074	3.175ArgLys: 3.175 ± 0.068	3.892ArgLeu: 3.892 ± 0.087	1.224ArgMet: 1.224 ± 0.041	2.127ArgAsn: 2.127 ± 0.059	1.175ArgPro: 1.175 ± 0.041	1.263ArgGln: 1.263 ± 0.04	1.992ArgArg: 1.992 ± 0.064	2.355ArgSer: 2.355 ± 0.066	2.203ArgThr: 2.203 ± 0.049	2.295ArgVal: 2.295 ± 0.053	0.301ArgTrp: 0.301 ± 0.021	1.975ArgTyr: 1.975 ± 0.061	0.001ArgXaa: 0.001 ± 0.002
Ser	5.715SerAla: 5.715 ± 0.102	1.031SerCys: 1.031 ± 0.041	5.077SerAsp: 5.077 ± 0.112	4.691SerGlu: 4.691 ± 0.083	2.843SerPhe: 2.843 ± 0.068	5.632SerGly: 5.632 ± 0.1	0.945SerHis: 0.945 ± 0.032	4.016SerIle: 4.016 ± 0.09	4.011SerLys: 4.011 ± 0.079	5.26SerLeu: 5.26 ± 0.096	1.591SerMet: 1.591 ± 0.043	2.517SerAsn: 2.517 ± 0.067	1.822SerPro: 1.822 ± 0.049	1.789SerGln: 1.789 ± 0.051	2.55SerArg: 2.55 ± 0.071	4.256SerSer: 4.256 ± 0.113	3.36SerThr: 3.36 ± 0.072	5.448SerVal: 5.448 ± 0.095	0.482SerTrp: 0.482 ± 0.026	2.698SerTyr: 2.698 ± 0.062	0.0SerXaa: 0.0 ± 0.0
Thr	6.46ThrAla: 6.46 ± 0.108	0.768ThrCys: 0.768 ± 0.032	4.588ThrAsp: 4.588 ± 0.082	3.963ThrGlu: 3.963 ± 0.072	2.295ThrPhe: 2.295 ± 0.065	4.834ThrGly: 4.834 ± 0.09	0.874ThrHis: 0.874 ± 0.033	3.706ThrIle: 3.706 ± 0.08	3.205ThrLys: 3.205 ± 0.071	5.27ThrLeu: 5.27 ± 0.097	1.351ThrMet: 1.351 ± 0.043	2.256ThrAsn: 2.256 ± 0.064	2.338ThrPro: 2.338 ± 0.061	1.496ThrGln: 1.496 ± 0.045	1.822ThrArg: 1.822 ± 0.052	3.469ThrSer: 3.469 ± 0.09	3.383ThrThr: 3.383 ± 0.096	5.876ThrVal: 5.876 ± 0.125	0.38ThrTrp: 0.38 ± 0.024	2.107ThrTyr: 2.107 ± 0.063	0.001ThrXaa: 0.001 ± 0.002
Val	4.928ValAla: 4.928 ± 0.096	1.379ValCys: 1.379 ± 0.05	3.821ValAsp: 3.821 ± 0.079	3.6ValGlu: 3.6 ± 0.073	3.024ValPhe: 3.024 ± 0.077	4.007ValGly: 4.007 ± 0.079	0.982ValHis: 0.982 ± 0.039	5.796ValIle: 5.796 ± 0.106	4.893ValLys: 4.893 ± 0.094	5.963ValLeu: 5.963 ± 0.101	2.131ValMet: 2.131 ± 0.055	3.346ValAsn: 3.346 ± 0.068	2.475ValPro: 2.475 ± 0.055	1.596ValGln: 1.596 ± 0.042	2.863ValArg: 2.863 ± 0.067	5.134ValSer: 5.134 ± 0.091	4.886ValThr: 4.886 ± 0.095	4.263ValVal: 4.263 ± 0.084	0.554ValTrp: 0.554 ± 0.028	2.765ValTyr: 2.765 ± 0.072	0.003ValXaa: 0.003 ± 0.002
Trp	0.475TrpAla: 0.475 ± 0.03	0.15TrpCys: 0.15 ± 0.016	0.43TrpAsp: 0.43 ± 0.024	0.442TrpGlu: 0.442 ± 0.026	0.366TrpPhe: 0.366 ± 0.024	0.613TrpGly: 0.613 ± 0.025	0.166TrpHis: 0.166 ± 0.016	0.493TrpIle: 0.493 ± 0.028	0.456TrpLys: 0.456 ± 0.026	0.783TrpLeu: 0.783 ± 0.036	0.177TrpMet: 0.177 ± 0.016	0.502TrpAsn: 0.502 ± 0.033	0.097TrpPro: 0.097 ± 0.012	0.327TrpGln: 0.327 ± 0.021	0.29TrpArg: 0.29 ± 0.019	0.501TrpSer: 0.501 ± 0.029	0.395TrpThr: 0.395 ± 0.024	0.411TrpVal: 0.411 ± 0.026	0.11TrpTrp: 0.11 ± 0.014	0.363TrpTyr: 0.363 ± 0.022	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.918TyrAla: 2.918 ± 0.071	0.806TyrCys: 0.806 ± 0.038	3.003TyrAsp: 3.003 ± 0.071	2.766TyrGlu: 2.766 ± 0.068	1.841TyrPhe: 1.841 ± 0.052	3.179TyrGly: 3.179 ± 0.069	0.63TyrHis: 0.63 ± 0.031	3.188TyrIle: 3.188 ± 0.064	2.791TyrLys: 2.791 ± 0.067	3.187TyrLeu: 3.187 ± 0.072	1.063TyrMet: 1.063 ± 0.035	2.235TyrAsn: 2.235 ± 0.067	1.423TyrPro: 1.423 ± 0.043	0.803TyrGln: 0.803 ± 0.033	1.782TyrArg: 1.782 ± 0.049	3.107TyrSer: 3.107 ± 0.076	2.732TyrThr: 2.732 ± 0.07	2.387TyrVal: 2.387 ± 0.061	0.29TyrTrp: 0.29 ± 0.02	1.91TyrTyr: 1.91 ± 0.053	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.001XaaPhe: 0.001 ± 0.002	0.001XaaGly: 0.001 ± 0.002	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.003XaaAsn: 0.003 ± 0.002	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.001XaaSer: 0.001 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.011XaaXaa: 0.011 ± 0.005

Statistics based on 2308 proteins (734533 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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