Sphingobium sp. YR768
Average proteome isoelectric point is 6.54
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5343 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1H9U428|A0A1H9U428_9SPHN Lipopolysaccharide export system protein LptA OS=Sphingobium sp. YR768 OX=1884365 GN=SAMN05518866_13326 PE=4 SV=1
MM1 pKa = 7.41 GEE3 pKa = 4.0 SVAIFDD9 pKa = 5.3 GGLEE13 pKa = 3.95 DD14 pKa = 6.82 DD15 pKa = 4.78 IYY17 pKa = 11.36 LGTKK21 pKa = 10.01 SSDD24 pKa = 3.28 IISGNGGNDD33 pKa = 3.17 ALVGDD38 pKa = 4.75 EE39 pKa = 5.88 GSDD42 pKa = 3.94 QIDD45 pKa = 3.66 GGDD48 pKa = 3.85 GNDD51 pKa = 3.44 FLYY54 pKa = 10.85 AQTLSFDD61 pKa = 2.98 WKK63 pKa = 10.5 GYY65 pKa = 6.88 NFRR68 pKa = 11.84 WLPPVTDD75 pKa = 3.21 VDD77 pKa = 4.19 AVKK80 pKa = 9.71 DD81 pKa = 3.85 TLNGGDD87 pKa = 3.95 GNDD90 pKa = 4.79 HH91 pKa = 6.72 ISAGYY96 pKa = 10.44 GDD98 pKa = 5.86 DD99 pKa = 3.45 IDD101 pKa = 5.05 GGEE104 pKa = 4.16 GSDD107 pKa = 3.51 YY108 pKa = 11.19 LVISFRR114 pKa = 11.84 GASSGIIADD123 pKa = 4.66 FSTLEE128 pKa = 4.03 TGGSVTVEE136 pKa = 3.59 GGTIKK141 pKa = 10.52 NVEE144 pKa = 3.99 MLEE147 pKa = 4.2 WVDD150 pKa = 3.66 GSEE153 pKa = 4.19 FSDD156 pKa = 4.3 VITGQIAASDD166 pKa = 3.77 APIYY170 pKa = 10.78 GLGGDD175 pKa = 3.97 DD176 pKa = 4.17 HH177 pKa = 7.79 LIAGGTGTGRR187 pKa = 11.84 LYY189 pKa = 11.14 GGDD192 pKa = 3.54 GDD194 pKa = 4.8 DD195 pKa = 4.03 TLEE198 pKa = 4.48 SAWSMLYY205 pKa = 10.6 GGSGNDD211 pKa = 3.98 LLIGGYY217 pKa = 9.28 PFAYY221 pKa = 10.26 GEE223 pKa = 5.0 DD224 pKa = 3.9 GDD226 pKa = 4.56 DD227 pKa = 5.03 VIWALNEE234 pKa = 3.86 ASGGAGDD241 pKa = 5.63 DD242 pKa = 3.36 IIGFLRR248 pKa = 11.84 DD249 pKa = 3.2 LDD251 pKa = 3.93 GSGRR255 pKa = 11.84 PGILYY260 pKa = 10.54 GDD262 pKa = 4.8 DD263 pKa = 3.61 GNDD266 pKa = 3.18 RR267 pKa = 11.84 LEE269 pKa = 4.53 GSGHH273 pKa = 6.1 IDD275 pKa = 3.21 KK276 pKa = 11.11 LSGGSGSDD284 pKa = 2.79 ILYY287 pKa = 10.94 GNGGDD292 pKa = 4.43 DD293 pKa = 3.35 ILYY296 pKa = 10.54 AGASGSGTGAFDD308 pKa = 4.37 PSDD311 pKa = 3.49 SLAEE315 pKa = 4.07 HH316 pKa = 6.89 DD317 pKa = 4.46 QLFGGDD323 pKa = 4.18 GDD325 pKa = 4.14 DD326 pKa = 4.65 VMIIGYY332 pKa = 10.43 GDD334 pKa = 3.91 DD335 pKa = 4.09 ADD337 pKa = 5.17 GGDD340 pKa = 3.86 GTDD343 pKa = 3.1 SVTVYY348 pKa = 11.44 ALGASSGVFIDD359 pKa = 5.34 IGDD362 pKa = 4.01 PSNGRR367 pKa = 11.84 IGTGIIRR374 pKa = 11.84 NVEE377 pKa = 4.0 IIIGVYY383 pKa = 10.51 ASDD386 pKa = 3.8 FDD388 pKa = 5.58 DD389 pKa = 4.6 VIKK392 pKa = 10.8 VDD394 pKa = 3.94 NVGSDD399 pKa = 3.18 ASINGKK405 pKa = 9.57 KK406 pKa = 10.72 GNDD409 pKa = 3.46 IISGLSHH416 pKa = 6.65 SVTFNGNEE424 pKa = 4.08 GNDD427 pKa = 3.48 ILIGSAQGDD436 pKa = 4.01 MLDD439 pKa = 4.27 GGSGNDD445 pKa = 3.4 IIYY448 pKa = 10.61 GGGGIDD454 pKa = 4.56 RR455 pKa = 11.84 ISGGDD460 pKa = 3.4 GDD462 pKa = 5.37 DD463 pKa = 3.99 VIEE466 pKa = 4.96 GGSGTNYY473 pKa = 9.68 IGGGAGIDD481 pKa = 3.77 TISYY485 pKa = 8.8 RR486 pKa = 11.84 HH487 pKa = 5.14 STGAVVVDD495 pKa = 4.24 LTPPDD500 pKa = 3.66 GATVKK505 pKa = 10.54 VQTPDD510 pKa = 3.4 SIDD513 pKa = 3.34 YY514 pKa = 10.4 FSTIEE519 pKa = 4.07 NVVGSQYY526 pKa = 11.32 GDD528 pKa = 3.76 SIKK531 pKa = 10.72 GDD533 pKa = 3.27 SGINVISGEE542 pKa = 4.46 DD543 pKa = 3.73 GDD545 pKa = 4.76 DD546 pKa = 3.95 TILGQDD552 pKa = 3.93 GADD555 pKa = 3.3 ILYY558 pKa = 10.59 GDD560 pKa = 5.03 AGNDD564 pKa = 3.42 VLGGGVGIDD573 pKa = 3.38 TLYY576 pKa = 11.33 GGTGNDD582 pKa = 3.81 RR583 pKa = 11.84 LDD585 pKa = 3.91 GGAGADD591 pKa = 3.42 RR592 pKa = 11.84 LVGGVGDD599 pKa = 5.4 DD600 pKa = 3.31 IYY602 pKa = 10.98 IVDD605 pKa = 3.74 TAGDD609 pKa = 4.15 VIVEE613 pKa = 4.13 LAGEE617 pKa = 4.58 GDD619 pKa = 3.62 DD620 pKa = 4.35 HH621 pKa = 7.9 VYY623 pKa = 11.17 SSVSYY628 pKa = 10.24 ILSSHH633 pKa = 6.27 IEE635 pKa = 4.08 RR636 pKa = 11.84 LSMTGTAAVDD646 pKa = 3.7 GTGNEE651 pKa = 4.13 LDD653 pKa = 3.67 NVIVGNAAANKK664 pKa = 8.39 LTGGTGNDD672 pKa = 3.73 YY673 pKa = 11.35 LDD675 pKa = 4.71 GLDD678 pKa = 5.28 GDD680 pKa = 5.09 DD681 pKa = 4.7 LLEE684 pKa = 4.83 GGAGNDD690 pKa = 3.13 ILIGGRR696 pKa = 11.84 GGDD699 pKa = 3.62 QMIGGAGDD707 pKa = 3.82 DD708 pKa = 4.57 LYY710 pKa = 11.76 VVDD713 pKa = 6.27 DD714 pKa = 3.76 IRR716 pKa = 11.84 DD717 pKa = 3.99 TVSEE721 pKa = 4.43 DD722 pKa = 2.96 ADD724 pKa = 3.69 AGIDD728 pKa = 3.68 TVQSSVSFVLGDD740 pKa = 3.6 NVEE743 pKa = 3.97 NLALGGTAALTGTGNALNNVMTGNSGVSTLLGGDD777 pKa = 3.83 GDD779 pKa = 4.37 DD780 pKa = 4.38 HH781 pKa = 7.45 LSSGVNGRR789 pKa = 11.84 GILVGGAGSDD799 pKa = 3.69 VLIGSAGNDD808 pKa = 3.45 SLNSGLSGVRR818 pKa = 11.84 DD819 pKa = 3.57 MGTEE823 pKa = 3.79 KK824 pKa = 10.92 DD825 pKa = 3.78 VLIAGAGDD833 pKa = 4.15 DD834 pKa = 4.39 GASAGYY840 pKa = 10.58 GDD842 pKa = 5.51 DD843 pKa = 3.54 VDD845 pKa = 4.83 GGEE848 pKa = 5.12 GYY850 pKa = 10.82 DD851 pKa = 3.44 SLSYY855 pKa = 11.22 SFGGASAGIDD865 pKa = 3.2 IRR867 pKa = 11.84 SSDD870 pKa = 3.92 LLGAGPHH877 pKa = 5.96 VIGGGTVQNFEE888 pKa = 4.45 RR889 pKa = 11.84 LSGLRR894 pKa = 11.84 GSEE897 pKa = 3.48 FDD899 pKa = 5.57 DD900 pKa = 4.18 YY901 pKa = 11.69 INYY904 pKa = 7.76 DD905 pKa = 3.44 TFYY908 pKa = 10.54 ISQLVIEE915 pKa = 5.14 AGAGNDD921 pKa = 3.62 TVIITGDD928 pKa = 3.76 APVKK932 pKa = 10.18 MLGGDD937 pKa = 3.42 GDD939 pKa = 4.02 DD940 pKa = 3.74 RR941 pKa = 11.84 LVVGYY946 pKa = 10.08 QPGNQFDD953 pKa = 4.12 GGAGSDD959 pKa = 3.56 TIDD962 pKa = 3.11 FRR964 pKa = 11.84 LYY966 pKa = 10.43 GEE968 pKa = 4.52 KK969 pKa = 9.58 VTVALGLNGANGNGGPYY986 pKa = 9.0 LTQLLNVEE994 pKa = 4.29 NVIGSSYY1001 pKa = 11.35 ADD1003 pKa = 3.57 GIVGNEE1009 pKa = 4.0 LANDD1013 pKa = 3.67 LRR1015 pKa = 11.84 GGDD1018 pKa = 3.96 GDD1020 pKa = 4.45 DD1021 pKa = 4.56 TILGQDD1027 pKa = 4.17 GADD1030 pKa = 3.69 KK1031 pKa = 11.06 LYY1033 pKa = 11.18 GDD1035 pKa = 5.13 AGNDD1039 pKa = 3.51 VLGGGAGIDD1048 pKa = 3.5 TLYY1051 pKa = 11.22 GGTGNDD1057 pKa = 3.81 RR1058 pKa = 11.84 LDD1060 pKa = 3.91 GGAGADD1066 pKa = 3.42 RR1067 pKa = 11.84 LVGGVGDD1074 pKa = 5.4 DD1075 pKa = 3.31 IYY1077 pKa = 10.98 IVDD1080 pKa = 3.74 TAGDD1084 pKa = 4.15 VIVEE1088 pKa = 4.12 LAGEE1092 pKa = 4.44 GNDD1095 pKa = 3.37 TVVASGSYY1103 pKa = 8.88 TLSDD1107 pKa = 3.19 HH1108 pKa = 7.62 VEE1110 pKa = 4.08 TLKK1113 pKa = 11.43 LNGTVGAADD1122 pKa = 3.54 MRR1124 pKa = 11.84 GNGQDD1129 pKa = 3.53 NLITAEE1135 pKa = 4.19 GASGSSALGIRR1146 pKa = 11.84 LFGLGGNDD1154 pKa = 3.15 MLLGSVAGDD1163 pKa = 3.49 VLDD1166 pKa = 4.77 GGTGNDD1172 pKa = 3.32 VMKK1175 pKa = 10.87 GGLGSDD1181 pKa = 4.26 LYY1183 pKa = 11.47 YY1184 pKa = 10.58 VDD1186 pKa = 4.8 SVGDD1190 pKa = 3.66 QIVEE1194 pKa = 4.08 GLNAGVDD1201 pKa = 3.97 HH1202 pKa = 6.38 VASSISYY1209 pKa = 8.18 TLGANVEE1216 pKa = 4.34 GLTLTGSANLNGIGNALSNAIFGNDD1241 pKa = 3.1 GNNYY1245 pKa = 9.11 LSGMGGRR1252 pKa = 11.84 DD1253 pKa = 3.08 TLYY1256 pKa = 10.66 GYY1258 pKa = 10.49 GGNDD1262 pKa = 3.08 VLVPGDD1268 pKa = 3.74 GNNVVDD1274 pKa = 4.68 GGVGYY1279 pKa = 10.57 DD1280 pKa = 3.79 LLLLTGAKK1288 pKa = 9.94 GSYY1291 pKa = 10.11 SYY1293 pKa = 11.45 LSANGSIYY1301 pKa = 10.72 LVGEE1305 pKa = 4.1 EE1306 pKa = 5.07 GATKK1310 pKa = 7.95 MTGVEE1315 pKa = 4.26 SVRR1318 pKa = 11.84 FADD1321 pKa = 3.95 GTVATGDD1328 pKa = 3.8 LASSLTAFDD1337 pKa = 3.58 GLRR1340 pKa = 11.84 YY1341 pKa = 9.01 IAGYY1345 pKa = 9.26 SDD1347 pKa = 3.5 LMAVFGTNAAAGVSHH1362 pKa = 5.95 YY1363 pKa = 10.51 VSSGFAEE1370 pKa = 4.4 GRR1372 pKa = 11.84 DD1373 pKa = 3.33 AKK1375 pKa = 11.21 SFDD1378 pKa = 3.81 PLDD1381 pKa = 4.21 YY1382 pKa = 10.7 IAGYY1386 pKa = 10.29 SDD1388 pKa = 3.8 LASAFGTDD1396 pKa = 2.96 AQAATRR1402 pKa = 11.84 HH1403 pKa = 5.84 YY1404 pKa = 8.95 ITTGAKK1410 pKa = 9.68 EE1411 pKa = 3.9 GRR1413 pKa = 11.84 NDD1415 pKa = 4.22 DD1416 pKa = 4.17 RR1417 pKa = 11.84 FDD1419 pKa = 3.97 ALDD1422 pKa = 3.66 YY1423 pKa = 10.39 LASYY1427 pKa = 10.9 ADD1429 pKa = 4.11 LSATLGADD1437 pKa = 3.37 EE1438 pKa = 4.33 EE1439 pKa = 4.73 AGARR1443 pKa = 11.84 HH1444 pKa = 6.15 YY1445 pKa = 10.2 LTIGKK1450 pKa = 8.89 AQGRR1454 pKa = 11.84 SDD1456 pKa = 4.38 DD1457 pKa = 4.37 GFDD1460 pKa = 3.41 GLQYY1464 pKa = 11.0 AAGYY1468 pKa = 10.43 ADD1470 pKa = 4.43 LAALFGSNDD1479 pKa = 3.42 DD1480 pKa = 3.48 AAARR1484 pKa = 11.84 HH1485 pKa = 5.44 YY1486 pKa = 10.08 IQYY1489 pKa = 10.07 GRR1491 pKa = 11.84 AEE1493 pKa = 4.09 GRR1495 pKa = 11.84 VADD1498 pKa = 4.22 KK1499 pKa = 10.93 FDD1501 pKa = 3.29 GLRR1504 pKa = 11.84 YY1505 pKa = 9.19 IASNADD1511 pKa = 4.13 LIVSIGDD1518 pKa = 3.52 NDD1520 pKa = 3.82 DD1521 pKa = 3.87 AAAKK1525 pKa = 10.04 HH1526 pKa = 5.55 YY1527 pKa = 10.05 LDD1529 pKa = 4.77 HH1530 pKa = 6.98 GFAEE1534 pKa = 4.67 HH1535 pKa = 7.57 RR1536 pKa = 11.84 STTSFDD1542 pKa = 3.42 ALKK1545 pKa = 10.52 YY1546 pKa = 10.17 AAANPDD1552 pKa = 3.06 LAAAFGSDD1560 pKa = 3.33 VEE1562 pKa = 4.81 ALTEE1566 pKa = 4.01 HH1567 pKa = 6.67 YY1568 pKa = 10.34 IDD1570 pKa = 3.8 FGYY1573 pKa = 10.74 YY1574 pKa = 6.32 EE1575 pKa = 4.5 HH1576 pKa = 7.73 RR1577 pKa = 11.84 AIAPAAAMVEE1587 pKa = 4.61 VIGG1590 pKa = 4.83
Molecular weight: 160.45 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.679
IPC2_protein 3.592
IPC_protein 3.668
Toseland 3.427
ProMoST 3.846
Dawson 3.681
Bjellqvist 3.834
Wikipedia 3.643
Rodwell 3.49
Grimsley 3.325
Solomon 3.681
Lehninger 3.643
Nozaki 3.783
DTASelect 4.113
Thurlkill 3.478
EMBOSS 3.656
Sillero 3.795
Patrickios 1.418
IPC_peptide 3.668
IPC2_peptide 3.77
IPC2.peptide.svr19 3.726
Protein with the highest isoelectric point:
>tr|A0A1H9KWK9|A0A1H9KWK9_9SPHN Proline iminopeptidase OS=Sphingobium sp. YR768 OX=1884365 GN=SAMN05518866_104189 PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATPGGRR28 pKa = 11.84 NVIRR32 pKa = 11.84 ARR34 pKa = 11.84 RR35 pKa = 11.84 SRR37 pKa = 11.84 GRR39 pKa = 11.84 KK40 pKa = 9.06 SLSAA44 pKa = 3.93
Molecular weight: 5.12 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.647
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.369
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.218
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5343
0
5343
1701863
24
2462
318.5
34.49
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.935 ± 0.05
0.794 ± 0.011
6.243 ± 0.025
5.017 ± 0.033
3.523 ± 0.024
8.845 ± 0.033
2.075 ± 0.019
5.239 ± 0.021
3.107 ± 0.027
9.803 ± 0.036
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.545 ± 0.018
2.656 ± 0.023
5.281 ± 0.026
3.338 ± 0.018
7.143 ± 0.034
5.448 ± 0.026
5.287 ± 0.031
6.858 ± 0.025
1.472 ± 0.015
2.393 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here