Sphingobium sp. YR768

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Sphingomonadaceae; Sphingobium; unclassified Sphingobium

Average proteome isoelectric point is 6.54

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5343 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A1H9U428|A0A1H9U428_9SPHN Lipopolysaccharide export system protein LptA OS=Sphingobium sp. YR768 OX=1884365 GN=SAMN05518866_13326 PE=4 SV=1
MM1 pKa = 7.41GEE3 pKa = 4.0SVAIFDD9 pKa = 5.3GGLEE13 pKa = 3.95DD14 pKa = 6.82DD15 pKa = 4.78IYY17 pKa = 11.36LGTKK21 pKa = 10.01SSDD24 pKa = 3.28IISGNGGNDD33 pKa = 3.17ALVGDD38 pKa = 4.75EE39 pKa = 5.88GSDD42 pKa = 3.94QIDD45 pKa = 3.66GGDD48 pKa = 3.85GNDD51 pKa = 3.44FLYY54 pKa = 10.85AQTLSFDD61 pKa = 2.98WKK63 pKa = 10.5GYY65 pKa = 6.88NFRR68 pKa = 11.84WLPPVTDD75 pKa = 3.21VDD77 pKa = 4.19AVKK80 pKa = 9.71DD81 pKa = 3.85TLNGGDD87 pKa = 3.95GNDD90 pKa = 4.79HH91 pKa = 6.72ISAGYY96 pKa = 10.44GDD98 pKa = 5.86DD99 pKa = 3.45IDD101 pKa = 5.05GGEE104 pKa = 4.16GSDD107 pKa = 3.51YY108 pKa = 11.19LVISFRR114 pKa = 11.84GASSGIIADD123 pKa = 4.66FSTLEE128 pKa = 4.03TGGSVTVEE136 pKa = 3.59GGTIKK141 pKa = 10.52NVEE144 pKa = 3.99MLEE147 pKa = 4.2WVDD150 pKa = 3.66GSEE153 pKa = 4.19FSDD156 pKa = 4.3VITGQIAASDD166 pKa = 3.77APIYY170 pKa = 10.78GLGGDD175 pKa = 3.97DD176 pKa = 4.17HH177 pKa = 7.79LIAGGTGTGRR187 pKa = 11.84LYY189 pKa = 11.14GGDD192 pKa = 3.54GDD194 pKa = 4.8DD195 pKa = 4.03TLEE198 pKa = 4.48SAWSMLYY205 pKa = 10.6GGSGNDD211 pKa = 3.98LLIGGYY217 pKa = 9.28PFAYY221 pKa = 10.26GEE223 pKa = 5.0DD224 pKa = 3.9GDD226 pKa = 4.56DD227 pKa = 5.03VIWALNEE234 pKa = 3.86ASGGAGDD241 pKa = 5.63DD242 pKa = 3.36IIGFLRR248 pKa = 11.84DD249 pKa = 3.2LDD251 pKa = 3.93GSGRR255 pKa = 11.84PGILYY260 pKa = 10.54GDD262 pKa = 4.8DD263 pKa = 3.61GNDD266 pKa = 3.18RR267 pKa = 11.84LEE269 pKa = 4.53GSGHH273 pKa = 6.1IDD275 pKa = 3.21KK276 pKa = 11.11LSGGSGSDD284 pKa = 2.79ILYY287 pKa = 10.94GNGGDD292 pKa = 4.43DD293 pKa = 3.35ILYY296 pKa = 10.54AGASGSGTGAFDD308 pKa = 4.37PSDD311 pKa = 3.49SLAEE315 pKa = 4.07HH316 pKa = 6.89DD317 pKa = 4.46QLFGGDD323 pKa = 4.18GDD325 pKa = 4.14DD326 pKa = 4.65VMIIGYY332 pKa = 10.43GDD334 pKa = 3.91DD335 pKa = 4.09ADD337 pKa = 5.17GGDD340 pKa = 3.86GTDD343 pKa = 3.1SVTVYY348 pKa = 11.44ALGASSGVFIDD359 pKa = 5.34IGDD362 pKa = 4.01PSNGRR367 pKa = 11.84IGTGIIRR374 pKa = 11.84NVEE377 pKa = 4.0IIIGVYY383 pKa = 10.51ASDD386 pKa = 3.8FDD388 pKa = 5.58DD389 pKa = 4.6VIKK392 pKa = 10.8VDD394 pKa = 3.94NVGSDD399 pKa = 3.18ASINGKK405 pKa = 9.57KK406 pKa = 10.72GNDD409 pKa = 3.46IISGLSHH416 pKa = 6.65SVTFNGNEE424 pKa = 4.08GNDD427 pKa = 3.48ILIGSAQGDD436 pKa = 4.01MLDD439 pKa = 4.27GGSGNDD445 pKa = 3.4IIYY448 pKa = 10.61GGGGIDD454 pKa = 4.56RR455 pKa = 11.84ISGGDD460 pKa = 3.4GDD462 pKa = 5.37DD463 pKa = 3.99VIEE466 pKa = 4.96GGSGTNYY473 pKa = 9.68IGGGAGIDD481 pKa = 3.77TISYY485 pKa = 8.8RR486 pKa = 11.84HH487 pKa = 5.14STGAVVVDD495 pKa = 4.24LTPPDD500 pKa = 3.66GATVKK505 pKa = 10.54VQTPDD510 pKa = 3.4SIDD513 pKa = 3.34YY514 pKa = 10.4FSTIEE519 pKa = 4.07NVVGSQYY526 pKa = 11.32GDD528 pKa = 3.76SIKK531 pKa = 10.72GDD533 pKa = 3.27SGINVISGEE542 pKa = 4.46DD543 pKa = 3.73GDD545 pKa = 4.76DD546 pKa = 3.95TILGQDD552 pKa = 3.93GADD555 pKa = 3.3ILYY558 pKa = 10.59GDD560 pKa = 5.03AGNDD564 pKa = 3.42VLGGGVGIDD573 pKa = 3.38TLYY576 pKa = 11.33GGTGNDD582 pKa = 3.81RR583 pKa = 11.84LDD585 pKa = 3.91GGAGADD591 pKa = 3.42RR592 pKa = 11.84LVGGVGDD599 pKa = 5.4DD600 pKa = 3.31IYY602 pKa = 10.98IVDD605 pKa = 3.74TAGDD609 pKa = 4.15VIVEE613 pKa = 4.13LAGEE617 pKa = 4.58GDD619 pKa = 3.62DD620 pKa = 4.35HH621 pKa = 7.9VYY623 pKa = 11.17SSVSYY628 pKa = 10.24ILSSHH633 pKa = 6.27IEE635 pKa = 4.08RR636 pKa = 11.84LSMTGTAAVDD646 pKa = 3.7GTGNEE651 pKa = 4.13LDD653 pKa = 3.67NVIVGNAAANKK664 pKa = 8.39LTGGTGNDD672 pKa = 3.73YY673 pKa = 11.35LDD675 pKa = 4.71GLDD678 pKa = 5.28GDD680 pKa = 5.09DD681 pKa = 4.7LLEE684 pKa = 4.83GGAGNDD690 pKa = 3.13ILIGGRR696 pKa = 11.84GGDD699 pKa = 3.62QMIGGAGDD707 pKa = 3.82DD708 pKa = 4.57LYY710 pKa = 11.76VVDD713 pKa = 6.27DD714 pKa = 3.76IRR716 pKa = 11.84DD717 pKa = 3.99TVSEE721 pKa = 4.43DD722 pKa = 2.96ADD724 pKa = 3.69AGIDD728 pKa = 3.68TVQSSVSFVLGDD740 pKa = 3.6NVEE743 pKa = 3.97NLALGGTAALTGTGNALNNVMTGNSGVSTLLGGDD777 pKa = 3.83GDD779 pKa = 4.37DD780 pKa = 4.38HH781 pKa = 7.45LSSGVNGRR789 pKa = 11.84GILVGGAGSDD799 pKa = 3.69VLIGSAGNDD808 pKa = 3.45SLNSGLSGVRR818 pKa = 11.84DD819 pKa = 3.57MGTEE823 pKa = 3.79KK824 pKa = 10.92DD825 pKa = 3.78VLIAGAGDD833 pKa = 4.15DD834 pKa = 4.39GASAGYY840 pKa = 10.58GDD842 pKa = 5.51DD843 pKa = 3.54VDD845 pKa = 4.83GGEE848 pKa = 5.12GYY850 pKa = 10.82DD851 pKa = 3.44SLSYY855 pKa = 11.22SFGGASAGIDD865 pKa = 3.2IRR867 pKa = 11.84SSDD870 pKa = 3.92LLGAGPHH877 pKa = 5.96VIGGGTVQNFEE888 pKa = 4.45RR889 pKa = 11.84LSGLRR894 pKa = 11.84GSEE897 pKa = 3.48FDD899 pKa = 5.57DD900 pKa = 4.18YY901 pKa = 11.69INYY904 pKa = 7.76DD905 pKa = 3.44TFYY908 pKa = 10.54ISQLVIEE915 pKa = 5.14AGAGNDD921 pKa = 3.62TVIITGDD928 pKa = 3.76APVKK932 pKa = 10.18MLGGDD937 pKa = 3.42GDD939 pKa = 4.02DD940 pKa = 3.74RR941 pKa = 11.84LVVGYY946 pKa = 10.08QPGNQFDD953 pKa = 4.12GGAGSDD959 pKa = 3.56TIDD962 pKa = 3.11FRR964 pKa = 11.84LYY966 pKa = 10.43GEE968 pKa = 4.52KK969 pKa = 9.58VTVALGLNGANGNGGPYY986 pKa = 9.0LTQLLNVEE994 pKa = 4.29NVIGSSYY1001 pKa = 11.35ADD1003 pKa = 3.57GIVGNEE1009 pKa = 4.0LANDD1013 pKa = 3.67LRR1015 pKa = 11.84GGDD1018 pKa = 3.96GDD1020 pKa = 4.45DD1021 pKa = 4.56TILGQDD1027 pKa = 4.17GADD1030 pKa = 3.69KK1031 pKa = 11.06LYY1033 pKa = 11.18GDD1035 pKa = 5.13AGNDD1039 pKa = 3.51VLGGGAGIDD1048 pKa = 3.5TLYY1051 pKa = 11.22GGTGNDD1057 pKa = 3.81RR1058 pKa = 11.84LDD1060 pKa = 3.91GGAGADD1066 pKa = 3.42RR1067 pKa = 11.84LVGGVGDD1074 pKa = 5.4DD1075 pKa = 3.31IYY1077 pKa = 10.98IVDD1080 pKa = 3.74TAGDD1084 pKa = 4.15VIVEE1088 pKa = 4.12LAGEE1092 pKa = 4.44GNDD1095 pKa = 3.37TVVASGSYY1103 pKa = 8.88TLSDD1107 pKa = 3.19HH1108 pKa = 7.62VEE1110 pKa = 4.08TLKK1113 pKa = 11.43LNGTVGAADD1122 pKa = 3.54MRR1124 pKa = 11.84GNGQDD1129 pKa = 3.53NLITAEE1135 pKa = 4.19GASGSSALGIRR1146 pKa = 11.84LFGLGGNDD1154 pKa = 3.15MLLGSVAGDD1163 pKa = 3.49VLDD1166 pKa = 4.77GGTGNDD1172 pKa = 3.32VMKK1175 pKa = 10.87GGLGSDD1181 pKa = 4.26LYY1183 pKa = 11.47YY1184 pKa = 10.58VDD1186 pKa = 4.8SVGDD1190 pKa = 3.66QIVEE1194 pKa = 4.08GLNAGVDD1201 pKa = 3.97HH1202 pKa = 6.38VASSISYY1209 pKa = 8.18TLGANVEE1216 pKa = 4.34GLTLTGSANLNGIGNALSNAIFGNDD1241 pKa = 3.1GNNYY1245 pKa = 9.11LSGMGGRR1252 pKa = 11.84DD1253 pKa = 3.08TLYY1256 pKa = 10.66GYY1258 pKa = 10.49GGNDD1262 pKa = 3.08VLVPGDD1268 pKa = 3.74GNNVVDD1274 pKa = 4.68GGVGYY1279 pKa = 10.57DD1280 pKa = 3.79LLLLTGAKK1288 pKa = 9.94GSYY1291 pKa = 10.11SYY1293 pKa = 11.45LSANGSIYY1301 pKa = 10.72LVGEE1305 pKa = 4.1EE1306 pKa = 5.07GATKK1310 pKa = 7.95MTGVEE1315 pKa = 4.26SVRR1318 pKa = 11.84FADD1321 pKa = 3.95GTVATGDD1328 pKa = 3.8LASSLTAFDD1337 pKa = 3.58GLRR1340 pKa = 11.84YY1341 pKa = 9.01IAGYY1345 pKa = 9.26SDD1347 pKa = 3.5LMAVFGTNAAAGVSHH1362 pKa = 5.95YY1363 pKa = 10.51VSSGFAEE1370 pKa = 4.4GRR1372 pKa = 11.84DD1373 pKa = 3.33AKK1375 pKa = 11.21SFDD1378 pKa = 3.81PLDD1381 pKa = 4.21YY1382 pKa = 10.7IAGYY1386 pKa = 10.29SDD1388 pKa = 3.8LASAFGTDD1396 pKa = 2.96AQAATRR1402 pKa = 11.84HH1403 pKa = 5.84YY1404 pKa = 8.95ITTGAKK1410 pKa = 9.68EE1411 pKa = 3.9GRR1413 pKa = 11.84NDD1415 pKa = 4.22DD1416 pKa = 4.17RR1417 pKa = 11.84FDD1419 pKa = 3.97ALDD1422 pKa = 3.66YY1423 pKa = 10.39LASYY1427 pKa = 10.9ADD1429 pKa = 4.11LSATLGADD1437 pKa = 3.37EE1438 pKa = 4.33EE1439 pKa = 4.73AGARR1443 pKa = 11.84HH1444 pKa = 6.15YY1445 pKa = 10.2LTIGKK1450 pKa = 8.89AQGRR1454 pKa = 11.84SDD1456 pKa = 4.38DD1457 pKa = 4.37GFDD1460 pKa = 3.41GLQYY1464 pKa = 11.0AAGYY1468 pKa = 10.43ADD1470 pKa = 4.43LAALFGSNDD1479 pKa = 3.42DD1480 pKa = 3.48AAARR1484 pKa = 11.84HH1485 pKa = 5.44YY1486 pKa = 10.08IQYY1489 pKa = 10.07GRR1491 pKa = 11.84AEE1493 pKa = 4.09GRR1495 pKa = 11.84VADD1498 pKa = 4.22KK1499 pKa = 10.93FDD1501 pKa = 3.29GLRR1504 pKa = 11.84YY1505 pKa = 9.19IASNADD1511 pKa = 4.13LIVSIGDD1518 pKa = 3.52NDD1520 pKa = 3.82DD1521 pKa = 3.87AAAKK1525 pKa = 10.04HH1526 pKa = 5.55YY1527 pKa = 10.05LDD1529 pKa = 4.77HH1530 pKa = 6.98GFAEE1534 pKa = 4.67HH1535 pKa = 7.57RR1536 pKa = 11.84STTSFDD1542 pKa = 3.42ALKK1545 pKa = 10.52YY1546 pKa = 10.17AAANPDD1552 pKa = 3.06LAAAFGSDD1560 pKa = 3.33VEE1562 pKa = 4.81ALTEE1566 pKa = 4.01HH1567 pKa = 6.67YY1568 pKa = 10.34IDD1570 pKa = 3.8FGYY1573 pKa = 10.74YY1574 pKa = 6.32EE1575 pKa = 4.5HH1576 pKa = 7.73RR1577 pKa = 11.84AIAPAAAMVEE1587 pKa = 4.61VIGG1590 pKa = 4.83

Molecular weight:
160.45 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A1H9KWK9|A0A1H9KWK9_9SPHN Proline iminopeptidase OS=Sphingobium sp. YR768 OX=1884365 GN=SAMN05518866_104189 PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATPGGRR28 pKa = 11.84NVIRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.06SLSAA44 pKa = 3.93

Molecular weight:
5.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5343

0

5343

1701863

24

2462

318.5

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.935 ± 0.05

0.794 ± 0.011

6.243 ± 0.025

5.017 ± 0.033

3.523 ± 0.024

8.845 ± 0.033

2.075 ± 0.019

5.239 ± 0.021

3.107 ± 0.027

9.803 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.545 ± 0.018

2.656 ± 0.023

5.281 ± 0.026

3.338 ± 0.018

7.143 ± 0.034

5.448 ± 0.026

5.287 ± 0.031

6.858 ± 0.025

1.472 ± 0.015

2.393 ± 0.019

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski