Influenza D virus (D/bovine/France/2986/2012)
Average proteome isoelectric point is 6.72
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 7 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0E4C2T3|A0A0E4C2T3_9ORTO p3 OS=Influenza D virus (D/bovine/France/2986/2012) OX=1542973 GN=P3 PE=4 SV=1
MM1 pKa = 7.43 FLLLATITAITACQAEE17 pKa = 4.47 RR18 pKa = 11.84 EE19 pKa = 4.52 LICIVQRR26 pKa = 11.84 VNEE29 pKa = 4.38 SFSLHH34 pKa = 6.32 SGFGGNVYY42 pKa = 10.76 SMKK45 pKa = 9.78 TEE47 pKa = 3.84 PMTGFTNVTKK57 pKa = 10.27 GASVINQKK65 pKa = 10.62 DD66 pKa = 3.32 WIGFGDD72 pKa = 4.17 SRR74 pKa = 11.84 TDD76 pKa = 3.16 LTNAQFPASSDD87 pKa = 3.52 VPLAVAKK94 pKa = 10.39 KK95 pKa = 9.46 FRR97 pKa = 11.84 SLSGASLMLSAFGPPDD113 pKa = 3.46 KK114 pKa = 10.91 VDD116 pKa = 3.68 YY117 pKa = 10.65 LYY119 pKa = 10.71 QGCGKK124 pKa = 10.19 EE125 pKa = 3.91 KK126 pKa = 10.66 VFYY129 pKa = 10.74 EE130 pKa = 4.47 GVNWSPEE137 pKa = 3.71 AGIDD141 pKa = 3.84 CFGSNWTQTKK151 pKa = 10.06 KK152 pKa = 10.77 DD153 pKa = 4.01 FYY155 pKa = 11.06 SRR157 pKa = 11.84 IYY159 pKa = 9.57 EE160 pKa = 3.98 AARR163 pKa = 11.84 GSTCMTLVNSLDD175 pKa = 3.78 TKK177 pKa = 10.63 ISSTTATAGTTSSCSSSWMKK197 pKa = 10.82 SPLWYY202 pKa = 10.36 AEE204 pKa = 3.96 SSVNPGASPQVCGTEE219 pKa = 3.81 QSAIFTLPTSFGIYY233 pKa = 9.38 KK234 pKa = 9.96 CNKK237 pKa = 9.4 HH238 pKa = 6.03 VVQLCYY244 pKa = 9.81 FVYY247 pKa = 10.38 EE248 pKa = 4.55 SKK250 pKa = 9.54 TAFNTFGCGDD260 pKa = 4.19 YY261 pKa = 10.37 YY262 pKa = 11.22 QNYY265 pKa = 9.53 YY266 pKa = 10.46 DD267 pKa = 4.84 GNGNLIGGMDD277 pKa = 3.91 NRR279 pKa = 11.84 VAAYY283 pKa = 10.04 RR284 pKa = 11.84 GIAGAGAKK292 pKa = 9.46 IEE294 pKa = 4.41 CPSKK298 pKa = 10.3 ILNPGTYY305 pKa = 9.9 SIRR308 pKa = 11.84 STPRR312 pKa = 11.84 FLLVPKK318 pKa = 10.37 RR319 pKa = 11.84 SYY321 pKa = 11.48 CFDD324 pKa = 3.44 TDD326 pKa = 2.79 GGYY329 pKa = 9.81 PIQVVQSEE337 pKa = 4.36 WSASRR342 pKa = 11.84 RR343 pKa = 11.84 SDD345 pKa = 3.09 NATEE349 pKa = 4.1 EE350 pKa = 4.53 ACLQTEE356 pKa = 4.29 GCIFIKK362 pKa = 9.79 KK363 pKa = 4.33 TTPYY367 pKa = 10.51 VGEE370 pKa = 4.65 ADD372 pKa = 4.66 DD373 pKa = 3.73 NHH375 pKa = 8.17 GDD377 pKa = 3.19 IEE379 pKa = 4.21 MRR381 pKa = 11.84 QLLSGLGNNDD391 pKa = 3.69 TVCVSQSGYY400 pKa = 9.38 TKK402 pKa = 11.12 GEE404 pKa = 4.05 TPFVKK409 pKa = 10.25 DD410 pKa = 3.32 YY411 pKa = 11.12 LSPPKK416 pKa = 10.44 YY417 pKa = 10.38 GRR419 pKa = 11.84 CQLKK423 pKa = 9.04 TDD425 pKa = 3.58 SGRR428 pKa = 11.84 IPTLPSGLIIPQAGTDD444 pKa = 3.32 SLMRR448 pKa = 11.84 TLTPATRR455 pKa = 11.84 IFGIDD460 pKa = 3.46 DD461 pKa = 5.14 LIFGLLFIGFVAGGVAGGYY480 pKa = 8.51 FWGRR484 pKa = 11.84 SSEE487 pKa = 4.23 GGGGASVSSTQAGFDD502 pKa = 4.2 KK503 pKa = 10.45 IGKK506 pKa = 9.3 DD507 pKa = 3.34 IQQLRR512 pKa = 11.84 SDD514 pKa = 3.68 TNAAIEE520 pKa = 4.27 GFNGRR525 pKa = 11.84 IAHH528 pKa = 7.12 DD529 pKa = 3.64 EE530 pKa = 3.99 QAVKK534 pKa = 10.83 NLAKK538 pKa = 10.26 EE539 pKa = 4.12 IEE541 pKa = 4.3 DD542 pKa = 3.64 ARR544 pKa = 11.84 AEE546 pKa = 4.09 ALVGEE551 pKa = 4.44 LGIIRR556 pKa = 11.84 SLIVANISMNLKK568 pKa = 9.73 EE569 pKa = 4.66 SLYY572 pKa = 10.74 EE573 pKa = 3.92 LANQITKK580 pKa = 10.42 RR581 pKa = 11.84 GGGIAQEE588 pKa = 4.44 AGPGCWYY595 pKa = 10.6 VDD597 pKa = 4.53 SEE599 pKa = 4.67 NCDD602 pKa = 3.46 ASCKK606 pKa = 10.28 EE607 pKa = 4.07 YY608 pKa = 10.57 IFNFNGSATVPTLRR622 pKa = 11.84 PVDD625 pKa = 3.66 TNVIITSDD633 pKa = 3.75 PYY635 pKa = 11.64 YY636 pKa = 10.56 LGSTIALCLLGLVAIAAFVGVIWICCKK663 pKa = 10.45 KK664 pKa = 10.73
Molecular weight: 71.59 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.188
IPC2_protein 5.474
IPC_protein 5.423
Toseland 5.359
ProMoST 5.601
Dawson 5.461
Bjellqvist 5.575
Wikipedia 5.347
Rodwell 5.385
Grimsley 5.296
Solomon 5.461
Lehninger 5.423
Nozaki 5.601
DTASelect 5.766
Thurlkill 5.461
EMBOSS 5.436
Sillero 5.677
Patrickios 3.401
IPC_peptide 5.461
IPC2_peptide 5.664
IPC2.peptide.svr19 5.654
Protein with the highest isoelectric point:
>tr|A0A0E4C2S8|A0A0E4C2S8_9ORTO PB2 OS=Influenza D virus (D/bovine/France/2986/2012) OX=1542973 GN=PB2 PE=4 SV=1
MM1 pKa = 8.3 DD2 pKa = 3.59 STKK5 pKa = 10.9 AQTPEE10 pKa = 3.5 EE11 pKa = 3.96 QRR13 pKa = 11.84 AKK15 pKa = 9.92 NAKK18 pKa = 9.12 TILEE22 pKa = 4.4 NIQIYY27 pKa = 10.6 EE28 pKa = 4.19 RR29 pKa = 11.84 MCDD32 pKa = 3.34 LFGVSEE38 pKa = 4.37 DD39 pKa = 3.87 DD40 pKa = 4.4 KK41 pKa = 11.8 LIIEE45 pKa = 4.25 NSISIEE51 pKa = 3.75 RR52 pKa = 11.84 MIRR55 pKa = 11.84 VVTDD59 pKa = 3.53 KK60 pKa = 10.94 KK61 pKa = 11.15 YY62 pKa = 10.68 QDD64 pKa = 3.89 KK65 pKa = 10.62 KK66 pKa = 10.92 LKK68 pKa = 10.42 NAGSDD73 pKa = 3.52 LEE75 pKa = 4.69 KK76 pKa = 10.33 IANAGKK82 pKa = 9.29 VFCRR86 pKa = 11.84 LVEE89 pKa = 4.33 STAGKK94 pKa = 10.19 CSARR98 pKa = 11.84 LGMALKK104 pKa = 10.48 PNVEE108 pKa = 4.11 AVLTDD113 pKa = 3.36 VLGNEE118 pKa = 4.47 LDD120 pKa = 3.58 RR121 pKa = 11.84 AAVLGKK127 pKa = 10.69 RR128 pKa = 11.84 MGFTAMFKK136 pKa = 10.97 SNLEE140 pKa = 3.77 EE141 pKa = 3.69 VLYY144 pKa = 10.89 QRR146 pKa = 11.84 GKK148 pKa = 9.78 NQLKK152 pKa = 9.94 KK153 pKa = 10.37 RR154 pKa = 11.84 NSAEE158 pKa = 4.17 TFTLSQGASLEE169 pKa = 4.05 ARR171 pKa = 11.84 FRR173 pKa = 11.84 PIMEE177 pKa = 3.92 KK178 pKa = 10.2 HH179 pKa = 6.02 LGVGTVVASIKK190 pKa = 10.84 NILASKK196 pKa = 10.75 KK197 pKa = 10.16 NGNYY201 pKa = 8.42 KK202 pKa = 10.65 NKK204 pKa = 9.35 MVRR207 pKa = 11.84 KK208 pKa = 8.61 PGGNRR213 pKa = 11.84 EE214 pKa = 3.96 SWSPLEE220 pKa = 4.41 RR221 pKa = 11.84 EE222 pKa = 3.84 ISFLNKK228 pKa = 9.93 KK229 pKa = 9.56 LFPGPMRR236 pKa = 11.84 QLCKK240 pKa = 10.45 KK241 pKa = 10.55 FEE243 pKa = 4.07 YY244 pKa = 11.12 LNDD247 pKa = 3.61 QEE249 pKa = 5.2 KK250 pKa = 10.37 QLALNLMLDD259 pKa = 3.5 ASLILKK265 pKa = 8.33 PQVTHH270 pKa = 6.86 KK271 pKa = 10.49 MIMPWSMWLAVKK283 pKa = 10.24 KK284 pKa = 9.74 YY285 pKa = 10.78 AEE287 pKa = 4.23 MNKK290 pKa = 9.89 GSPSLEE296 pKa = 3.86 DD297 pKa = 3.12 LAAYY301 pKa = 10.16 SGVRR305 pKa = 11.84 AFMAFNTACYY315 pKa = 9.03 MSKK318 pKa = 9.15 FTIGKK323 pKa = 9.63 GIVGDD328 pKa = 3.95 AEE330 pKa = 3.97 IMEE333 pKa = 4.57 NGNDD337 pKa = 3.51 KK338 pKa = 9.86 MQILAMACFGLAYY351 pKa = 9.73 EE352 pKa = 4.54 DD353 pKa = 3.77 TGIVAAMISQPMKK366 pKa = 10.25 KK367 pKa = 9.71 RR368 pKa = 11.84 YY369 pKa = 7.14 QLKK372 pKa = 9.76 VGNFNPPEE380 pKa = 4.11 EE381 pKa = 4.33 GTIKK385 pKa = 9.77 GTSAGYY391 pKa = 6.91 FHH393 pKa = 7.42 KK394 pKa = 10.11 WAEE397 pKa = 4.05 FGNRR401 pKa = 11.84 LPFNSFGTGEE411 pKa = 4.3 SKK413 pKa = 10.73 QISNSGVFAVQRR425 pKa = 11.84 PSTTNIQRR433 pKa = 11.84 LAEE436 pKa = 3.98 LMARR440 pKa = 11.84 NTGEE444 pKa = 3.77 TSDD447 pKa = 4.85 NFTQLVQKK455 pKa = 10.37 IRR457 pKa = 11.84 EE458 pKa = 4.18 QVGTFADD465 pKa = 3.6 QKK467 pKa = 10.78 ANLRR471 pKa = 11.84 EE472 pKa = 3.95 FTGGYY477 pKa = 9.5 IYY479 pKa = 10.36 DD480 pKa = 3.59 ITDD483 pKa = 3.38 VTKK486 pKa = 10.73 SNPKK490 pKa = 9.71 IPQLGGNSFFFEE502 pKa = 4.45 FTGSDD507 pKa = 3.7 VPRR510 pKa = 11.84 TGAKK514 pKa = 9.49 RR515 pKa = 11.84 RR516 pKa = 11.84 VGGADD521 pKa = 3.6 DD522 pKa = 3.83 VTLGTSQPKK531 pKa = 9.75 KK532 pKa = 9.96 RR533 pKa = 11.84 GRR535 pKa = 11.84 QGAGVEE541 pKa = 3.94 SSMDD545 pKa = 3.15 IEE547 pKa = 4.89 TVGEE551 pKa = 4.13 DD552 pKa = 3.08
Molecular weight: 61.31 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.866
IPC2_protein 8.682
IPC_protein 8.551
Toseland 9.516
ProMoST 9.151
Dawson 9.721
Bjellqvist 9.399
Wikipedia 9.838
Rodwell 10.189
Grimsley 9.765
Solomon 9.75
Lehninger 9.721
Nozaki 9.604
DTASelect 9.355
Thurlkill 9.589
EMBOSS 9.926
Sillero 9.663
Patrickios 5.995
IPC_peptide 9.75
IPC2_peptide 7.863
IPC2.peptide.svr19 7.927
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
7
0
7
4081
243
772
583.0
65.77
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.641 ± 0.606
2.23 ± 0.27
4.631 ± 0.215
8.233 ± 0.805
4.631 ± 0.428
7.131 ± 0.726
1.054 ± 0.188
6.028 ± 0.209
8.013 ± 0.573
8.233 ± 0.388
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
3.994 ± 0.724
4.729 ± 0.309
3.651 ± 0.154
2.965 ± 0.262
5.587 ± 0.413
6.714 ± 0.459
5.954 ± 0.404
5.342 ± 0.388
1.201 ± 0.134
3.038 ± 0.232
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here