Cellulomonas sp. WB94

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Micrococcales; Cellulomonadaceae; Cellulomonas; unclassified Cellulomonas

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3376 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2T9XMW1|A0A2T9XMW1_9CELL Redoxin OS=Cellulomonas sp. WB94 OX=2173174 GN=DDP54_16035 PE=4 SV=1
MM1 pKa = 8.02GDD3 pKa = 3.51PSLPGPMRR11 pKa = 11.84RR12 pKa = 11.84IPVKK16 pKa = 10.49LSWRR20 pKa = 11.84VGATMLAVAAVVAVPVPALADD41 pKa = 3.64TVAVTNAVVAGDD53 pKa = 3.7GSKK56 pKa = 10.33SVRR59 pKa = 11.84TDD61 pKa = 2.83TGVGVTVSYY70 pKa = 10.04TLTAEE75 pKa = 4.06NNPVGDD81 pKa = 3.97LQGCNAQTKK90 pKa = 8.71PGGGVTVTILPAANVTASPSALIFTDD116 pKa = 4.08CSTAQSVTFSASSPGDD132 pKa = 3.5YY133 pKa = 10.62SVTSSATGGKK143 pKa = 7.88TGSLFTTTASNFTLHH158 pKa = 6.16VVSPNQPPTVTVSGVLQGASYY179 pKa = 10.76EE180 pKa = 4.36HH181 pKa = 6.84GSVPAAMCNVTDD193 pKa = 3.8DD194 pKa = 4.22HH195 pKa = 7.4DD196 pKa = 3.97VARR199 pKa = 11.84TFAATLSAITGTLTSYY215 pKa = 11.61GLGGRR220 pKa = 11.84TATCAATDD228 pKa = 3.69TGGLTSTTAASYY240 pKa = 9.45TIVDD244 pKa = 3.7TTRR247 pKa = 11.84PILTVPGDD255 pKa = 3.86LTAEE259 pKa = 4.24ATSSSGAVVTWSASATDD276 pKa = 4.21AVDD279 pKa = 4.0GSPAVACSGAGGLTSGATFPLGATLVGCTATDD311 pKa = 3.46VAGNQATQQFQVTVQDD327 pKa = 3.64TTPPLLTLPASSTTEE342 pKa = 3.56ATGPSGASVTYY353 pKa = 10.14SASATDD359 pKa = 4.4SVDD362 pKa = 3.48GDD364 pKa = 3.98LTPTCTPASATTFGITTTTVDD385 pKa = 3.49CTATDD390 pKa = 2.95AHH392 pKa = 6.77ANHH395 pKa = 6.51AQGLFSVTVQDD406 pKa = 3.69TTPPTLTLPTVAPRR420 pKa = 11.84EE421 pKa = 4.22ATGPSGATVTYY432 pKa = 10.19AATASDD438 pKa = 4.32VVDD441 pKa = 3.38LTVPVTCDD449 pKa = 3.26YY450 pKa = 7.89PTGSVFQVGDD460 pKa = 3.65TTVSCTATDD469 pKa = 3.23SHH471 pKa = 7.08GNTTPGHH478 pKa = 5.06FTVTVTDD485 pKa = 3.36TTGPVLTLPGNISEE499 pKa = 4.78EE500 pKa = 4.34ATKK503 pKa = 9.85STGSDD508 pKa = 2.4IAYY511 pKa = 10.29SVLASDD517 pKa = 5.29LVDD520 pKa = 3.39GTVATSCDD528 pKa = 3.46PANGTFAIGTTTVTCTATDD547 pKa = 3.05AHH549 pKa = 6.92ANTTTGSFTVTVVDD563 pKa = 3.72TTAPVFSAGPGDD575 pKa = 3.84QTLEE579 pKa = 3.8AAGADD584 pKa = 3.55GTVATFASPTAVDD597 pKa = 4.71LVDD600 pKa = 3.56GDD602 pKa = 4.1RR603 pKa = 11.84AVLCVSSSGLGSGSTFPLGTTTVTCVASDD632 pKa = 3.36TRR634 pKa = 11.84GNVSDD639 pKa = 3.3QTFTVKK645 pKa = 10.39VSDD648 pKa = 4.4TIAPLVGVLEE658 pKa = 4.22NLTPEE663 pKa = 3.85ATGPDD668 pKa = 3.62GAVVSFTAPTATDD681 pKa = 3.46AVSGSPAVTCDD692 pKa = 3.41PASGSTFAIDD702 pKa = 3.32TVATITCSATDD713 pKa = 3.25SSRR716 pKa = 11.84NTGSSTFTVVVHH728 pKa = 7.21DD729 pKa = 3.89STGPALAVPANITEE743 pKa = 4.45EE744 pKa = 4.13ATSPDD749 pKa = 3.14GAAASWTDD757 pKa = 3.31ASATDD762 pKa = 4.62LVDD765 pKa = 4.25GPLPATCDD773 pKa = 3.49PASGSVFPLGNATAVTCTAYY793 pKa = 10.25DD794 pKa = 3.46VRR796 pKa = 11.84HH797 pKa = 5.73NKK799 pKa = 9.52GVGGFTVTVLDD810 pKa = 3.84RR811 pKa = 11.84TAPTLAVSGDD821 pKa = 3.65SALEE825 pKa = 3.73ATAPSGAPATFTATATDD842 pKa = 3.84LVDD845 pKa = 4.32GVVTPTCTAGGDD857 pKa = 4.0PVASGATFPLGTTTVTCSATDD878 pKa = 3.18SHH880 pKa = 6.89GNNAPSQSFTITVQDD895 pKa = 3.41TTRR898 pKa = 11.84PSVTVPATMTLEE910 pKa = 4.08ATSANGAPGTFASSATDD927 pKa = 3.63LVDD930 pKa = 3.51GTLTPTCTPASGATFPLGATTVTCSATDD958 pKa = 3.13AHH960 pKa = 7.05ANTATGSFAVTVVDD974 pKa = 3.74TTAPVVSVPANVATFATSAAGAVVTFAAPTASDD1007 pKa = 3.44AVGGNSLAVCTPPSGSLFKK1026 pKa = 10.96PGDD1029 pKa = 3.82TVVSCTATDD1038 pKa = 3.41AAGNSASRR1046 pKa = 11.84TFKK1049 pKa = 10.94VIVRR1053 pKa = 11.84FDD1055 pKa = 3.27WTGFFAPVDD1064 pKa = 3.72NAILNGMKK1072 pKa = 10.2AGSTAPIKK1080 pKa = 9.91WQVSNQGGGYY1090 pKa = 10.05VPDD1093 pKa = 3.56LTIVSKK1099 pKa = 7.34TTSGIAICATGALVDD1114 pKa = 4.32DD1115 pKa = 4.97LEE1117 pKa = 5.66AYY1119 pKa = 8.06STGGTQLRR1127 pKa = 11.84YY1128 pKa = 10.49DD1129 pKa = 3.75STAHH1133 pKa = 4.81QFIYY1137 pKa = 10.34NWQSPKK1143 pKa = 10.68KK1144 pKa = 9.87PGTCYY1149 pKa = 10.36TIIIGLTDD1157 pKa = 3.8GTSHH1161 pKa = 7.22SATFQLKK1168 pKa = 9.86

Molecular weight: 115.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2T9XQX5|A0A2T9XQX5_9CELL DUF5666 domain-containing protein OS=Cellulomonas sp. WB94 OX=2173174 GN=DDP54_05220 PE=4 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.42KK7 pKa = 8.47RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.22RR11 pKa = 11.84MAKK14 pKa = 9.41KK15 pKa = 9.87KK16 pKa = 9.7HH17 pKa = 5.84RR18 pKa = 11.84KK19 pKa = 8.56LLRR22 pKa = 11.84KK23 pKa = 7.78TRR25 pKa = 11.84HH26 pKa = 3.65QRR28 pKa = 11.84RR29 pKa = 11.84NKK31 pKa = 9.78KK32 pKa = 9.85

Molecular weight: 4.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3376

0

3376

1112575

32

1819

329.6

34.94

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.233 ± 0.067

0.599 ± 0.011

6.209 ± 0.033

4.908 ± 0.032

2.636 ± 0.025

9.245 ± 0.039

2.124 ± 0.021

3.541 ± 0.026

1.606 ± 0.027

10.376 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.685 ± 0.018

1.642 ± 0.022

5.707 ± 0.036

2.692 ± 0.021

7.604 ± 0.05

5.416 ± 0.03

6.509 ± 0.038

9.937 ± 0.04

1.488 ± 0.018

1.844 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski