Amino acid dipepetide frequency for Pseudomonas phage 67PfluR64PP

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
10.558AlaAla: 10.558 ± 0.987
1.048AlaCys: 1.048 ± 0.252
7.092AlaAsp: 7.092 ± 0.8
6.206AlaGlu: 6.206 ± 0.707
3.304AlaPhe: 3.304 ± 0.513
7.253AlaGly: 7.253 ± 0.606
2.176AlaHis: 2.176 ± 0.471
3.143AlaIle: 3.143 ± 0.426
6.206AlaLys: 6.206 ± 0.68
9.107AlaLeu: 9.107 ± 0.888
3.707AlaMet: 3.707 ± 0.627
3.466AlaAsn: 3.466 ± 0.642
2.901AlaPro: 2.901 ± 0.437
5.319AlaGln: 5.319 ± 0.748
6.044AlaArg: 6.044 ± 0.631
5.642AlaSer: 5.642 ± 0.742
4.916AlaThr: 4.916 ± 0.882
7.415AlaVal: 7.415 ± 0.684
1.209AlaTrp: 1.209 ± 0.445
2.176AlaTyr: 2.176 ± 0.39
0.0AlaXaa: 0.0 ± 0.0
Cys
1.209CysAla: 1.209 ± 0.308
0.0CysCys: 0.0 ± 0.0
0.645CysAsp: 0.645 ± 0.355
0.564CysGlu: 0.564 ± 0.254
0.403CysPhe: 0.403 ± 0.209
0.564CysGly: 0.564 ± 0.229
0.161CysHis: 0.161 ± 0.091
0.484CysIle: 0.484 ± 0.222
0.645CysLys: 0.645 ± 0.178
0.645CysLeu: 0.645 ± 0.23
0.161CysMet: 0.161 ± 0.105
0.322CysAsn: 0.322 ± 0.152
0.322CysPro: 0.322 ± 0.16
0.322CysGln: 0.322 ± 0.162
0.645CysArg: 0.645 ± 0.263
0.242CysSer: 0.242 ± 0.174
0.242CysThr: 0.242 ± 0.127
0.484CysVal: 0.484 ± 0.205
0.161CysTrp: 0.161 ± 0.142
0.242CysTyr: 0.242 ± 0.152
0.0CysXaa: 0.0 ± 0.0
Asp
7.334AspAla: 7.334 ± 0.725
0.484AspCys: 0.484 ± 0.211
4.271AspAsp: 4.271 ± 0.666
3.788AspGlu: 3.788 ± 0.599
3.143AspPhe: 3.143 ± 0.444
7.173AspGly: 7.173 ± 0.783
1.289AspHis: 1.289 ± 0.328
3.304AspIle: 3.304 ± 0.486
2.901AspLys: 2.901 ± 0.556
4.836AspLeu: 4.836 ± 0.668
1.612AspMet: 1.612 ± 0.315
1.854AspAsn: 1.854 ± 0.282
2.66AspPro: 2.66 ± 0.466
2.74AspGln: 2.74 ± 0.447
3.868AspArg: 3.868 ± 0.611
3.063AspSer: 3.063 ± 0.564
2.821AspThr: 2.821 ± 0.549
4.271AspVal: 4.271 ± 0.553
1.209AspTrp: 1.209 ± 0.314
1.692AspTyr: 1.692 ± 0.339
0.0AspXaa: 0.0 ± 0.0
Glu
8.785GluAla: 8.785 ± 0.813
1.048GluCys: 1.048 ± 0.329
3.949GluAsp: 3.949 ± 0.554
5.158GluGlu: 5.158 ± 0.816
2.821GluPhe: 2.821 ± 0.518
5.319GluGly: 5.319 ± 0.711
1.37GluHis: 1.37 ± 0.347
3.304GluIle: 3.304 ± 0.568
2.337GluLys: 2.337 ± 0.499
5.722GluLeu: 5.722 ± 0.635
1.289GluMet: 1.289 ± 0.365
2.418GluAsn: 2.418 ± 0.325
2.257GluPro: 2.257 ± 0.438
2.498GluGln: 2.498 ± 0.407
4.271GluArg: 4.271 ± 0.633
3.224GluSer: 3.224 ± 0.452
3.788GluThr: 3.788 ± 0.384
4.594GluVal: 4.594 ± 0.506
1.128GluTrp: 1.128 ± 0.302
2.095GluTyr: 2.095 ± 0.308
0.0GluXaa: 0.0 ± 0.0
Phe
2.337PheAla: 2.337 ± 0.483
0.322PheCys: 0.322 ± 0.237
2.982PheAsp: 2.982 ± 0.396
2.095PheGlu: 2.095 ± 0.428
1.37PhePhe: 1.37 ± 0.304
2.74PheGly: 2.74 ± 0.381
1.209PheHis: 1.209 ± 0.245
1.209PheIle: 1.209 ± 0.292
2.257PheLys: 2.257 ± 0.464
4.271PheLeu: 4.271 ± 0.586
1.209PheMet: 1.209 ± 0.244
1.934PheAsn: 1.934 ± 0.377
1.934PhePro: 1.934 ± 0.411
1.37PheGln: 1.37 ± 0.273
1.692PheArg: 1.692 ± 0.341
2.095PheSer: 2.095 ± 0.405
2.257PheThr: 2.257 ± 0.422
2.66PheVal: 2.66 ± 0.477
0.484PheTrp: 0.484 ± 0.205
0.725PheTyr: 0.725 ± 0.281
0.0PheXaa: 0.0 ± 0.0
Gly
7.656GlyAla: 7.656 ± 1.025
0.806GlyCys: 0.806 ± 0.275
5.239GlyAsp: 5.239 ± 0.77
4.836GlyGlu: 4.836 ± 0.633
3.788GlyPhe: 3.788 ± 0.666
6.85GlyGly: 6.85 ± 0.892
2.337GlyHis: 2.337 ± 0.407
4.352GlyIle: 4.352 ± 0.743
5.077GlyLys: 5.077 ± 0.982
6.044GlyLeu: 6.044 ± 0.654
2.176GlyMet: 2.176 ± 0.392
2.66GlyAsn: 2.66 ± 0.411
2.257GlyPro: 2.257 ± 0.485
2.498GlyGln: 2.498 ± 0.383
5.722GlyArg: 5.722 ± 0.875
5.803GlySer: 5.803 ± 0.605
5.077GlyThr: 5.077 ± 0.682
4.997GlyVal: 4.997 ± 0.541
1.451GlyTrp: 1.451 ± 0.322
2.982GlyTyr: 2.982 ± 0.521
0.0GlyXaa: 0.0 ± 0.0
His
1.612HisAla: 1.612 ± 0.416
0.242HisCys: 0.242 ± 0.124
1.531HisAsp: 1.531 ± 0.4
2.176HisGlu: 2.176 ± 0.338
1.209HisPhe: 1.209 ± 0.277
2.257HisGly: 2.257 ± 0.547
0.403HisHis: 0.403 ± 0.154
1.048HisIle: 1.048 ± 0.344
1.531HisLys: 1.531 ± 0.3
2.095HisLeu: 2.095 ± 0.275
0.806HisMet: 0.806 ± 0.299
1.128HisAsn: 1.128 ± 0.323
0.725HisPro: 0.725 ± 0.235
0.645HisGln: 0.645 ± 0.199
1.209HisArg: 1.209 ± 0.318
1.37HisSer: 1.37 ± 0.351
0.806HisThr: 0.806 ± 0.246
1.531HisVal: 1.531 ± 0.35
0.564HisTrp: 0.564 ± 0.166
0.645HisTyr: 0.645 ± 0.237
0.0HisXaa: 0.0 ± 0.0
Ile
3.788IleAla: 3.788 ± 0.548
0.564IleCys: 0.564 ± 0.176
2.901IleAsp: 2.901 ± 0.486
2.901IleGlu: 2.901 ± 0.456
0.806IlePhe: 0.806 ± 0.255
3.868IleGly: 3.868 ± 0.444
1.128IleHis: 1.128 ± 0.36
1.773IleIle: 1.773 ± 0.327
3.466IleLys: 3.466 ± 0.612
4.513IleLeu: 4.513 ± 0.485
1.531IleMet: 1.531 ± 0.407
0.967IleAsn: 0.967 ± 0.34
2.66IlePro: 2.66 ± 0.51
2.579IleGln: 2.579 ± 0.493
2.176IleArg: 2.176 ± 0.308
1.451IleSer: 1.451 ± 0.317
2.821IleThr: 2.821 ± 0.408
2.982IleVal: 2.982 ± 0.571
0.564IleTrp: 0.564 ± 0.175
0.967IleTyr: 0.967 ± 0.221
0.0IleXaa: 0.0 ± 0.0
Lys
6.689LysAla: 6.689 ± 0.852
0.403LysCys: 0.403 ± 0.177
4.271LysAsp: 4.271 ± 0.779
3.546LysGlu: 3.546 ± 0.657
1.854LysPhe: 1.854 ± 0.333
5.561LysGly: 5.561 ± 0.763
1.37LysHis: 1.37 ± 0.41
2.418LysIle: 2.418 ± 0.374
3.466LysLys: 3.466 ± 0.892
5.077LysLeu: 5.077 ± 0.642
1.934LysMet: 1.934 ± 0.276
1.451LysAsn: 1.451 ± 0.411
2.74LysPro: 2.74 ± 0.478
2.257LysGln: 2.257 ± 0.46
3.788LysArg: 3.788 ± 0.644
2.901LysSer: 2.901 ± 0.413
3.466LysThr: 3.466 ± 0.656
4.352LysVal: 4.352 ± 0.722
0.403LysTrp: 0.403 ± 0.141
1.773LysTyr: 1.773 ± 0.425
0.0LysXaa: 0.0 ± 0.0
Leu
8.704LeuAla: 8.704 ± 0.81
0.564LeuCys: 0.564 ± 0.207
4.433LeuAsp: 4.433 ± 0.582
6.689LeuGlu: 6.689 ± 0.778
2.901LeuPhe: 2.901 ± 0.505
4.755LeuGly: 4.755 ± 0.648
1.773LeuHis: 1.773 ± 0.282
4.352LeuIle: 4.352 ± 0.588
7.012LeuLys: 7.012 ± 0.69
4.836LeuLeu: 4.836 ± 0.625
2.418LeuMet: 2.418 ± 0.412
4.433LeuAsn: 4.433 ± 0.605
3.063LeuPro: 3.063 ± 0.413
4.03LeuGln: 4.03 ± 0.6
5.077LeuArg: 5.077 ± 0.601
4.271LeuSer: 4.271 ± 0.637
5.642LeuThr: 5.642 ± 0.581
6.286LeuVal: 6.286 ± 0.546
1.128LeuTrp: 1.128 ± 0.36
2.418LeuTyr: 2.418 ± 0.408
0.0LeuXaa: 0.0 ± 0.0
Met
3.385MetAla: 3.385 ± 0.507
0.161MetCys: 0.161 ± 0.111
1.692MetAsp: 1.692 ± 0.315
2.337MetGlu: 2.337 ± 0.432
0.645MetPhe: 0.645 ± 0.232
2.821MetGly: 2.821 ± 0.482
0.887MetHis: 0.887 ± 0.306
1.531MetIle: 1.531 ± 0.301
1.451MetLys: 1.451 ± 0.354
2.418MetLeu: 2.418 ± 0.409
0.645MetMet: 0.645 ± 0.25
0.645MetAsn: 0.645 ± 0.212
1.451MetPro: 1.451 ± 0.32
0.725MetGln: 0.725 ± 0.233
1.37MetArg: 1.37 ± 0.316
2.337MetSer: 2.337 ± 0.472
2.176MetThr: 2.176 ± 0.452
2.337MetVal: 2.337 ± 0.338
0.403MetTrp: 0.403 ± 0.162
0.484MetTyr: 0.484 ± 0.164
0.0MetXaa: 0.0 ± 0.0
Asn
3.466AsnAla: 3.466 ± 0.507
0.161AsnCys: 0.161 ± 0.11
1.612AsnAsp: 1.612 ± 0.295
2.015AsnGlu: 2.015 ± 0.383
1.451AsnPhe: 1.451 ± 0.337
3.788AsnGly: 3.788 ± 0.502
0.645AsnHis: 0.645 ± 0.244
1.451AsnIle: 1.451 ± 0.348
1.289AsnLys: 1.289 ± 0.283
4.03AsnLeu: 4.03 ± 0.455
1.531AsnMet: 1.531 ± 0.292
0.645AsnAsn: 0.645 ± 0.173
2.418AsnPro: 2.418 ± 0.5
1.37AsnGln: 1.37 ± 0.256
2.418AsnArg: 2.418 ± 0.568
1.451AsnSer: 1.451 ± 0.407
1.692AsnThr: 1.692 ± 0.323
2.257AsnVal: 2.257 ± 0.477
0.322AsnTrp: 0.322 ± 0.15
1.209AsnTyr: 1.209 ± 0.394
0.0AsnXaa: 0.0 ± 0.0
Pro
2.498ProAla: 2.498 ± 0.486
0.0ProCys: 0.0 ± 0.0
3.063ProAsp: 3.063 ± 0.576
3.707ProGlu: 3.707 ± 0.552
1.692ProPhe: 1.692 ± 0.299
2.66ProGly: 2.66 ± 0.565
1.289ProHis: 1.289 ± 0.321
0.887ProIle: 0.887 ± 0.252
2.579ProLys: 2.579 ± 0.403
2.418ProLeu: 2.418 ± 0.396
0.806ProMet: 0.806 ± 0.234
2.257ProAsn: 2.257 ± 0.299
1.048ProPro: 1.048 ± 0.288
1.854ProGln: 1.854 ± 0.316
2.015ProArg: 2.015 ± 0.327
2.176ProSer: 2.176 ± 0.42
2.176ProThr: 2.176 ± 0.367
2.821ProVal: 2.821 ± 0.459
0.564ProTrp: 0.564 ± 0.268
1.048ProTyr: 1.048 ± 0.309
0.0ProXaa: 0.0 ± 0.0
Gln
5.48GlnAla: 5.48 ± 0.843
0.081GlnCys: 0.081 ± 0.067
2.74GlnAsp: 2.74 ± 0.545
2.337GlnGlu: 2.337 ± 0.469
1.854GlnPhe: 1.854 ± 0.33
3.868GlnGly: 3.868 ± 0.52
0.967GlnHis: 0.967 ± 0.315
1.612GlnIle: 1.612 ± 0.311
2.015GlnLys: 2.015 ± 0.555
4.03GlnLeu: 4.03 ± 0.622
1.531GlnMet: 1.531 ± 0.414
1.128GlnAsn: 1.128 ± 0.316
0.806GlnPro: 0.806 ± 0.23
2.257GlnGln: 2.257 ± 0.42
2.579GlnArg: 2.579 ± 0.604
2.095GlnSer: 2.095 ± 0.371
1.934GlnThr: 1.934 ± 0.279
3.304GlnVal: 3.304 ± 0.563
0.725GlnTrp: 0.725 ± 0.275
1.531GlnTyr: 1.531 ± 0.469
0.0GlnXaa: 0.0 ± 0.0
Arg
4.755ArgAla: 4.755 ± 0.552
0.484ArgCys: 0.484 ± 0.227
4.191ArgAsp: 4.191 ± 0.609
4.352ArgGlu: 4.352 ± 0.724
2.418ArgPhe: 2.418 ± 0.432
4.836ArgGly: 4.836 ± 0.632
1.209ArgHis: 1.209 ± 0.359
3.143ArgIle: 3.143 ± 0.395
3.063ArgLys: 3.063 ± 0.47
4.997ArgLeu: 4.997 ± 0.539
1.531ArgMet: 1.531 ± 0.306
2.579ArgAsn: 2.579 ± 0.423
1.854ArgPro: 1.854 ± 0.486
2.66ArgGln: 2.66 ± 0.448
3.385ArgArg: 3.385 ± 0.468
4.513ArgSer: 4.513 ± 0.664
2.66ArgThr: 2.66 ± 0.439
3.707ArgVal: 3.707 ± 0.588
0.725ArgTrp: 0.725 ± 0.259
1.934ArgTyr: 1.934 ± 0.305
0.0ArgXaa: 0.0 ± 0.0
Ser
5.4SerAla: 5.4 ± 0.732
0.484SerCys: 0.484 ± 0.194
4.513SerAsp: 4.513 ± 0.486
2.821SerGlu: 2.821 ± 0.459
2.257SerPhe: 2.257 ± 0.341
5.4SerGly: 5.4 ± 0.627
1.692SerHis: 1.692 ± 0.312
2.66SerIle: 2.66 ± 0.447
3.949SerLys: 3.949 ± 0.539
4.594SerLeu: 4.594 ± 0.412
1.37SerMet: 1.37 ± 0.372
1.531SerAsn: 1.531 ± 0.442
1.854SerPro: 1.854 ± 0.353
1.854SerGln: 1.854 ± 0.436
2.821SerArg: 2.821 ± 0.348
2.901SerSer: 2.901 ± 0.57
2.176SerThr: 2.176 ± 0.39
4.513SerVal: 4.513 ± 0.574
0.725SerTrp: 0.725 ± 0.231
2.176SerTyr: 2.176 ± 0.467
0.0SerXaa: 0.0 ± 0.0
Thr
4.352ThrAla: 4.352 ± 0.552
0.484ThrCys: 0.484 ± 0.215
2.66ThrAsp: 2.66 ± 0.521
4.191ThrGlu: 4.191 ± 0.463
1.612ThrPhe: 1.612 ± 0.319
5.077ThrGly: 5.077 ± 0.681
1.209ThrHis: 1.209 ± 0.227
2.982ThrIle: 2.982 ± 0.507
4.271ThrLys: 4.271 ± 0.628
5.561ThrLeu: 5.561 ± 0.704
1.854ThrMet: 1.854 ± 0.34
1.612ThrAsn: 1.612 ± 0.397
2.66ThrPro: 2.66 ± 0.441
2.418ThrGln: 2.418 ± 0.563
2.418ThrArg: 2.418 ± 0.409
2.821ThrSer: 2.821 ± 0.682
3.949ThrThr: 3.949 ± 0.607
3.627ThrVal: 3.627 ± 0.586
0.322ThrTrp: 0.322 ± 0.14
1.854ThrTyr: 1.854 ± 0.296
0.0ThrXaa: 0.0 ± 0.0
Val
6.528ValAla: 6.528 ± 0.705
0.564ValCys: 0.564 ± 0.23
3.788ValAsp: 3.788 ± 0.549
5.4ValGlu: 5.4 ± 0.771
2.337ValPhe: 2.337 ± 0.474
4.271ValGly: 4.271 ± 0.586
1.531ValHis: 1.531 ± 0.407
3.143ValIle: 3.143 ± 0.465
3.304ValLys: 3.304 ± 0.46
6.367ValLeu: 6.367 ± 0.664
1.692ValMet: 1.692 ± 0.358
2.901ValAsn: 2.901 ± 0.502
2.74ValPro: 2.74 ± 0.429
3.304ValGln: 3.304 ± 0.463
4.191ValArg: 4.191 ± 0.711
4.513ValSer: 4.513 ± 0.505
5.077ValThr: 5.077 ± 0.566
4.836ValVal: 4.836 ± 0.741
1.048ValTrp: 1.048 ± 0.235
2.176ValTyr: 2.176 ± 0.468
0.0ValXaa: 0.0 ± 0.0
Trp
1.612TrpAla: 1.612 ± 0.273
0.081TrpCys: 0.081 ± 0.093
0.484TrpAsp: 0.484 ± 0.217
0.645TrpGlu: 0.645 ± 0.182
0.564TrpPhe: 0.564 ± 0.272
1.209TrpGly: 1.209 ± 0.243
0.322TrpHis: 0.322 ± 0.167
0.322TrpIle: 0.322 ± 0.133
1.048TrpLys: 1.048 ± 0.297
1.37TrpLeu: 1.37 ± 0.36
0.403TrpMet: 0.403 ± 0.167
0.484TrpAsn: 0.484 ± 0.19
0.322TrpPro: 0.322 ± 0.13
0.806TrpGln: 0.806 ± 0.198
0.806TrpArg: 0.806 ± 0.198
1.37TrpSer: 1.37 ± 0.384
0.645TrpThr: 0.645 ± 0.212
1.048TrpVal: 1.048 ± 0.351
0.081TrpTrp: 0.081 ± 0.08
0.242TrpTyr: 0.242 ± 0.156
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.74TyrAla: 2.74 ± 0.484
0.484TyrCys: 0.484 ± 0.192
2.257TyrAsp: 2.257 ± 0.386
1.612TyrGlu: 1.612 ± 0.323
0.725TyrPhe: 0.725 ± 0.207
2.337TyrGly: 2.337 ± 0.403
0.564TyrHis: 0.564 ± 0.196
1.289TyrIle: 1.289 ± 0.323
1.854TyrLys: 1.854 ± 0.439
1.854TyrLeu: 1.854 ± 0.401
1.612TyrMet: 1.612 ± 0.353
0.806TyrAsn: 0.806 ± 0.317
0.887TyrPro: 0.887 ± 0.248
1.451TyrGln: 1.451 ± 0.368
2.498TyrArg: 2.498 ± 0.457
1.451TyrSer: 1.451 ± 0.36
1.612TyrThr: 1.612 ± 0.335
1.692TyrVal: 1.692 ± 0.425
0.645TyrTrp: 0.645 ± 0.208
0.645TyrTyr: 0.645 ± 0.244
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 51 proteins (12409 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski