Candidatus Ornithobacterium hominis
Average proteome isoelectric point is 6.67
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1744 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A383U4G2|A0A383U4G2_9FLAO Cell wall-associated polypeptide CWBP200 OS=Candidatus Ornithobacterium hominis OX=2497989 GN=wapA_5 PE=4 SV=1
MM1 pKa = 7.78 AIKK4 pKa = 9.16 ITDD7 pKa = 3.28 EE8 pKa = 4.77 CINCGACEE16 pKa = 4.03 PEE18 pKa = 4.51 CPNNAIYY25 pKa = 10.14 EE26 pKa = 4.31 GAMDD30 pKa = 3.56 WRR32 pKa = 11.84 FSDD35 pKa = 3.67 GTDD38 pKa = 3.07 AQGFFVGLNGHH49 pKa = 5.98 SADD52 pKa = 4.2 ADD54 pKa = 3.68 APQAPISDD62 pKa = 4.15 DD63 pKa = 3.93 YY64 pKa = 11.91 YY65 pKa = 11.22 FIVPDD70 pKa = 3.79 KK71 pKa = 10.54 CTEE74 pKa = 3.99 CKK76 pKa = 10.48 GFHH79 pKa = 7.5 DD80 pKa = 5.05 EE81 pKa = 4.19 PQCAAVCPVDD91 pKa = 3.96 CCVPDD96 pKa = 3.8 EE97 pKa = 4.19 DD98 pKa = 5.44 HH99 pKa = 7.17 EE100 pKa = 4.31 EE101 pKa = 4.34 SEE103 pKa = 5.5 DD104 pKa = 3.74 RR105 pKa = 11.84 LLEE108 pKa = 4.34 KK109 pKa = 10.6 KK110 pKa = 10.78 AMLHH114 pKa = 6.11 HH115 pKa = 7.07
Molecular weight: 12.67 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.961
IPC2_protein 4.101
IPC_protein 4.062
Toseland 3.872
ProMoST 4.202
Dawson 4.05
Bjellqvist 4.202
Wikipedia 3.961
Rodwell 3.897
Grimsley 3.783
Solomon 4.037
Lehninger 3.986
Nozaki 4.164
DTASelect 4.368
Thurlkill 3.91
EMBOSS 3.973
Sillero 4.19
Patrickios 0.413
IPC_peptide 4.037
IPC2_peptide 4.164
IPC2.peptide.svr19 4.075
Protein with the highest isoelectric point:
>tr|A0A383U3G8|A0A383U3G8_9FLAO PUA domain (Predicted RNA-binding domain) OS=Candidatus Ornithobacterium hominis OX=2497989 GN=SAMEA104719789_01383 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNRR10 pKa = 11.84 KK11 pKa = 8.89 RR12 pKa = 11.84 RR13 pKa = 11.84 NKK15 pKa = 9.89 HH16 pKa = 3.92 GFRR19 pKa = 11.84 EE20 pKa = 4.1 RR21 pKa = 11.84 MSTKK25 pKa = 10.23 NGRR28 pKa = 11.84 KK29 pKa = 8.58 VLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 9.73 GRR39 pKa = 11.84 KK40 pKa = 8.42 RR41 pKa = 11.84 LTVSAVRR48 pKa = 11.84 AKK50 pKa = 10.51 RR51 pKa = 3.34
Molecular weight: 6.16 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.473
IPC2_protein 11.038
IPC_protein 12.618
Toseland 12.793
ProMoST 13.276
Dawson 12.793
Bjellqvist 12.778
Wikipedia 13.261
Rodwell 12.501
Grimsley 12.822
Solomon 13.276
Lehninger 13.188
Nozaki 12.793
DTASelect 12.778
Thurlkill 12.793
EMBOSS 13.29
Sillero 12.793
Patrickios 12.223
IPC_peptide 13.29
IPC2_peptide 12.266
IPC2.peptide.svr19 9.086
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1744
0
1744
576101
30
3062
330.3
37.55
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.321 ± 0.053
0.668 ± 0.018
5.185 ± 0.042
7.284 ± 0.062
5.403 ± 0.05
6.227 ± 0.05
1.888 ± 0.027
7.987 ± 0.06
8.246 ± 0.066
9.467 ± 0.067
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.178 ± 0.029
6.236 ± 0.064
3.475 ± 0.032
4.038 ± 0.037
3.654 ± 0.038
6.17 ± 0.047
4.816 ± 0.036
5.638 ± 0.041
1.061 ± 0.019
4.057 ± 0.042
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here