Microbacterium phage Burro

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Podoviridae; Burrovirus; Microbacterium virus Burro

Average proteome isoelectric point is 5.51

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 49 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A386KMN1|A0A386KMN1_9CAUD Uncharacterized protein OS=Microbacterium phage Burro OX=2315703 GN=46 PE=4 SV=1
MM1 pKa = 7.56SEE3 pKa = 3.76NDD5 pKa = 3.31EE6 pKa = 4.63TVVNTAIVDD15 pKa = 3.93TSPGAIVAATFEE27 pKa = 4.43KK28 pKa = 10.68PEE30 pKa = 4.01PLKK33 pKa = 10.84RR34 pKa = 11.84QWFLYY39 pKa = 9.84AAAAILLVATVILGSAFITNLTDD62 pKa = 4.0RR63 pKa = 11.84NNRR66 pKa = 11.84LNNVIVSQQDD76 pKa = 3.43SLHH79 pKa = 6.53EE80 pKa = 4.19KK81 pKa = 10.6DD82 pKa = 3.74EE83 pKa = 4.37QIEE86 pKa = 4.23EE87 pKa = 4.23LTATSQALYY96 pKa = 10.58DD97 pKa = 3.77QLLLAGEE104 pKa = 4.61TPDD107 pKa = 3.6EE108 pKa = 4.39PRR110 pKa = 11.84PPDD113 pKa = 3.92VVTGPAGDD121 pKa = 3.68RR122 pKa = 11.84GATGEE127 pKa = 4.24TGEE130 pKa = 4.61TGPPGPAGPVGPPGSQGEE148 pKa = 4.19PGVAGSSGNQGEE160 pKa = 4.35PGATGPSGPAGPAGPQGEE178 pKa = 4.5PGSPGATGPAGQSAFPFTFTFTYY201 pKa = 10.55LGIPYY206 pKa = 8.58TCTVTDD212 pKa = 3.5NTTAAACSITPP223 pKa = 3.7

Molecular weight: 22.5 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A386KL89|A0A386KL89_9CAUD Uncharacterized protein OS=Microbacterium phage Burro OX=2315703 GN=49 PE=4 SV=1
MM1 pKa = 7.65AKK3 pKa = 10.13KK4 pKa = 8.85KK5 pKa = 9.33TEE7 pKa = 4.38TIGEE11 pKa = 4.17RR12 pKa = 11.84NKK14 pKa = 10.79RR15 pKa = 11.84LGNAPGTGSVLKK27 pKa = 10.25PGYY30 pKa = 6.92TAPASPWGSLGNFLNPKK47 pKa = 8.77KK48 pKa = 10.03QVPVAPQNPRR58 pKa = 11.84TVTTAPINDD67 pKa = 4.04GGGSPGGGTGGGDD80 pKa = 3.04GGYY83 pKa = 10.47AAARR87 pKa = 11.84SASRR91 pKa = 11.84SASQKK96 pKa = 10.54QNDD99 pKa = 3.41NTAALVDD106 pKa = 3.94QQRR109 pKa = 11.84KK110 pKa = 9.68LIDD113 pKa = 3.42AFGTQRR119 pKa = 11.84DD120 pKa = 4.03KK121 pKa = 11.56KK122 pKa = 10.66LGNINNSFTTSDD134 pKa = 3.43SLLMKK139 pKa = 10.59NYY141 pKa = 10.08GLAAEE146 pKa = 4.81GLAGTRR152 pKa = 11.84RR153 pKa = 11.84QNEE156 pKa = 4.08MAEE159 pKa = 4.22GDD161 pKa = 3.47ASFSNISNAVRR172 pKa = 11.84EE173 pKa = 4.21RR174 pKa = 11.84QNIADD179 pKa = 3.65QAASQGAGEE188 pKa = 4.21TDD190 pKa = 3.65LLRR193 pKa = 11.84SQLAAFRR200 pKa = 11.84NYY202 pKa = 10.24AANQGEE208 pKa = 4.65VNRR211 pKa = 11.84SFNDD215 pKa = 3.41TLQSINNSLTSLNSDD230 pKa = 3.18TSTSRR235 pKa = 11.84TNLFNQRR242 pKa = 11.84EE243 pKa = 4.35ADD245 pKa = 5.2RR246 pKa = 11.84EE247 pKa = 4.28AAWANYY253 pKa = 9.57ANQTSDD259 pKa = 2.93AWTQIANIEE268 pKa = 4.13NANTNTEE275 pKa = 3.51SDD277 pKa = 3.41MTVGYY282 pKa = 10.11NKK284 pKa = 10.46KK285 pKa = 8.79FTDD288 pKa = 3.62AGAQAAAAVKK298 pKa = 10.45NSYY301 pKa = 10.08SRR303 pKa = 11.84QAIPTGWNEE312 pKa = 3.54WGGKK316 pKa = 9.26QGTQEE321 pKa = 3.97RR322 pKa = 11.84ALTSSNRR329 pKa = 11.84AAAISLGGPVRR340 pKa = 11.84RR341 pKa = 11.84PEE343 pKa = 4.09GATLRR348 pKa = 11.84RR349 pKa = 11.84WEE351 pKa = 4.15GG352 pKa = 3.07

Molecular weight: 37.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

49

0

49

17156

32

4491

350.1

38.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.154 ± 0.505

0.373 ± 0.12

6.499 ± 0.216

6.01 ± 0.342

3.241 ± 0.139

8.166 ± 0.359

1.399 ± 0.195

4.494 ± 0.249

4.162 ± 0.301

8.335 ± 0.274

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.536 ± 0.18

4.506 ± 0.236

5.077 ± 0.173

4.558 ± 0.212

5.59 ± 0.284

6.254 ± 0.239

6.96 ± 0.434

6.75 ± 0.24

1.9 ± 0.134

3.037 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski