Amino acid dipepetide frequency for Streptomyces sp. NA02950

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
21.546AlaAla: 21.546 ± 0.143
1.089AlaCys: 1.089 ± 0.023
8.48AlaAsp: 8.48 ± 0.064
9.235AlaGlu: 9.235 ± 0.088
3.382AlaPhe: 3.382 ± 0.042
12.619AlaGly: 12.619 ± 0.089
3.007AlaHis: 3.007 ± 0.031
3.45AlaIle: 3.45 ± 0.041
2.723AlaLys: 2.723 ± 0.057
14.605AlaLeu: 14.605 ± 0.113
2.486AlaMet: 2.486 ± 0.038
1.796AlaAsn: 1.796 ± 0.031
7.262AlaPro: 7.262 ± 0.068
3.655AlaGln: 3.655 ± 0.044
10.865AlaArg: 10.865 ± 0.083
5.725AlaSer: 5.725 ± 0.051
7.008AlaThr: 7.008 ± 0.061
12.325AlaVal: 12.325 ± 0.081
1.834AlaTrp: 1.834 ± 0.031
2.691AlaTyr: 2.691 ± 0.036
0.0AlaXaa: 0.0 ± 0.0
Cys
1.194CysAla: 1.194 ± 0.021
0.115CysCys: 0.115 ± 0.008
0.524CysAsp: 0.524 ± 0.017
0.424CysGlu: 0.424 ± 0.014
0.229CysPhe: 0.229 ± 0.009
1.024CysGly: 1.024 ± 0.021
0.211CysHis: 0.211 ± 0.01
0.164CysIle: 0.164 ± 0.009
0.12CysLys: 0.12 ± 0.007
0.769CysLeu: 0.769 ± 0.018
0.127CysMet: 0.127 ± 0.007
0.139CysAsn: 0.139 ± 0.007
0.575CysPro: 0.575 ± 0.015
0.187CysGln: 0.187 ± 0.008
0.698CysArg: 0.698 ± 0.02
0.442CysSer: 0.442 ± 0.014
0.521CysThr: 0.521 ± 0.017
0.696CysVal: 0.696 ± 0.018
0.128CysTrp: 0.128 ± 0.007
0.155CysTyr: 0.155 ± 0.008
0.0CysXaa: 0.0 ± 0.0
Asp
7.676AspAla: 7.676 ± 0.068
0.433AspCys: 0.433 ± 0.013
3.531AspAsp: 3.531 ± 0.043
3.843AspGlu: 3.843 ± 0.042
1.581AspPhe: 1.581 ± 0.031
6.464AspGly: 6.464 ± 0.06
1.513AspHis: 1.513 ± 0.025
1.941AspIle: 1.941 ± 0.028
1.114AspLys: 1.114 ± 0.026
6.021AspLeu: 6.021 ± 0.061
0.801AspMet: 0.801 ± 0.019
0.909AspAsn: 0.909 ± 0.017
4.623AspPro: 4.623 ± 0.049
1.6AspGln: 1.6 ± 0.027
5.239AspArg: 5.239 ± 0.051
2.487AspSer: 2.487 ± 0.034
3.394AspThr: 3.394 ± 0.037
4.393AspVal: 4.393 ± 0.047
1.032AspTrp: 1.032 ± 0.021
1.126AspTyr: 1.126 ± 0.021
0.0AspXaa: 0.0 ± 0.0
Glu
7.494GluAla: 7.494 ± 0.073
0.411GluCys: 0.411 ± 0.014
2.957GluAsp: 2.957 ± 0.039
3.763GluGlu: 3.763 ± 0.051
1.446GluPhe: 1.446 ± 0.026
4.415GluGly: 4.415 ± 0.048
1.574GluHis: 1.574 ± 0.023
2.235GluIle: 2.235 ± 0.031
1.346GluLys: 1.346 ± 0.029
7.166GluLeu: 7.166 ± 0.074
0.904GluMet: 0.904 ± 0.021
0.987GluAsn: 0.987 ± 0.021
3.537GluPro: 3.537 ± 0.042
2.084GluGln: 2.084 ± 0.032
6.114GluArg: 6.114 ± 0.061
2.541GluSer: 2.541 ± 0.035
2.965GluThr: 2.965 ± 0.038
4.657GluVal: 4.657 ± 0.046
0.78GluTrp: 0.78 ± 0.014
1.046GluTyr: 1.046 ± 0.023
0.0GluXaa: 0.0 ± 0.0
Phe
3.541PheAla: 3.541 ± 0.038
0.275PheCys: 0.275 ± 0.009
1.909PheAsp: 1.909 ± 0.028
1.398PheGlu: 1.398 ± 0.024
0.846PhePhe: 0.846 ± 0.018
2.968PheGly: 2.968 ± 0.039
0.62PheHis: 0.62 ± 0.015
0.757PheIle: 0.757 ± 0.018
0.454PheLys: 0.454 ± 0.018
2.48PheLeu: 2.48 ± 0.033
0.371PheMet: 0.371 ± 0.012
0.543PheAsn: 0.543 ± 0.015
1.38PhePro: 1.38 ± 0.023
0.663PheGln: 0.663 ± 0.016
1.905PheArg: 1.905 ± 0.03
1.409PheSer: 1.409 ± 0.026
1.961PheThr: 1.961 ± 0.029
2.025PheVal: 2.025 ± 0.027
0.397PheTrp: 0.397 ± 0.013
0.521PheTyr: 0.521 ± 0.014
0.0PheXaa: 0.0 ± 0.0
Gly
11.269GlyAla: 11.269 ± 0.073
0.935GlyCys: 0.935 ± 0.019
5.218GlyAsp: 5.218 ± 0.04
5.307GlyGlu: 5.307 ± 0.052
2.801GlyPhe: 2.801 ± 0.034
9.26GlyGly: 9.26 ± 0.099
2.52GlyHis: 2.52 ± 0.035
3.322GlyIle: 3.322 ± 0.038
2.362GlyLys: 2.362 ± 0.042
9.289GlyLeu: 9.289 ± 0.071
2.017GlyMet: 2.017 ± 0.03
1.624GlyAsn: 1.624 ± 0.031
5.593GlyPro: 5.593 ± 0.058
2.716GlyGln: 2.716 ± 0.036
8.246GlyArg: 8.246 ± 0.059
5.313GlySer: 5.313 ± 0.052
6.162GlyThr: 6.162 ± 0.062
7.417GlyVal: 7.417 ± 0.07
1.709GlyTrp: 1.709 ± 0.027
2.274GlyTyr: 2.274 ± 0.027
0.0GlyXaa: 0.0 ± 0.0
His
2.731HisAla: 2.731 ± 0.034
0.235HisCys: 0.235 ± 0.009
1.368HisAsp: 1.368 ± 0.024
1.226HisGlu: 1.226 ± 0.021
0.652HisPhe: 0.652 ± 0.018
2.583HisGly: 2.583 ± 0.032
0.816HisHis: 0.816 ± 0.02
0.757HisIle: 0.757 ± 0.017
0.35HisLys: 0.35 ± 0.01
2.472HisLeu: 2.472 ± 0.034
0.331HisMet: 0.331 ± 0.012
0.363HisAsn: 0.363 ± 0.011
1.997HisPro: 1.997 ± 0.034
0.74HisGln: 0.74 ± 0.02
2.311HisArg: 2.311 ± 0.033
1.076HisSer: 1.076 ± 0.02
1.535HisThr: 1.535 ± 0.024
1.585HisVal: 1.585 ± 0.025
0.395HisTrp: 0.395 ± 0.011
0.477HisTyr: 0.477 ± 0.012
0.0HisXaa: 0.0 ± 0.0
Ile
4.763IleAla: 4.763 ± 0.053
0.311IleCys: 0.311 ± 0.011
2.221IleAsp: 2.221 ± 0.031
1.994IleGlu: 1.994 ± 0.029
0.696IlePhe: 0.696 ± 0.021
3.542IleGly: 3.542 ± 0.043
0.617IleHis: 0.617 ± 0.015
0.844IleIle: 0.844 ± 0.024
0.718IleLys: 0.718 ± 0.018
2.254IleLeu: 2.254 ± 0.035
0.423IleMet: 0.423 ± 0.013
0.699IleAsn: 0.699 ± 0.018
1.748IlePro: 1.748 ± 0.029
0.697IleGln: 0.697 ± 0.017
2.405IleArg: 2.405 ± 0.034
1.732IleSer: 1.732 ± 0.026
2.257IleThr: 2.257 ± 0.037
2.564IleVal: 2.564 ± 0.037
0.38IleTrp: 0.38 ± 0.011
0.506IleTyr: 0.506 ± 0.015
0.0IleXaa: 0.0 ± 0.0
Lys
2.769LysAla: 2.769 ± 0.05
0.109LysCys: 0.109 ± 0.006
1.256LysAsp: 1.256 ± 0.033
1.148LysGlu: 1.148 ± 0.028
0.377LysPhe: 0.377 ± 0.013
1.832LysGly: 1.832 ± 0.038
0.427LysHis: 0.427 ± 0.016
0.792LysIle: 0.792 ± 0.02
0.873LysLys: 0.873 ± 0.029
1.88LysLeu: 1.88 ± 0.035
0.339LysMet: 0.339 ± 0.013
0.492LysAsn: 0.492 ± 0.018
1.285LysPro: 1.285 ± 0.029
0.629LysGln: 0.629 ± 0.02
1.546LysArg: 1.546 ± 0.029
1.089LysSer: 1.089 ± 0.024
1.232LysThr: 1.232 ± 0.03
1.744LysVal: 1.744 ± 0.033
0.247LysTrp: 0.247 ± 0.009
0.394LysTyr: 0.394 ± 0.014
0.0LysXaa: 0.0 ± 0.0
Leu
14.787LeuAla: 14.787 ± 0.101
0.92LeuCys: 0.92 ± 0.019
6.597LeuAsp: 6.597 ± 0.068
5.061LeuGlu: 5.061 ± 0.052
2.58LeuPhe: 2.58 ± 0.038
9.17LeuGly: 9.17 ± 0.069
2.376LeuHis: 2.376 ± 0.028
3.296LeuIle: 3.296 ± 0.038
1.865LeuLys: 1.865 ± 0.032
10.968LeuLeu: 10.968 ± 0.092
1.645LeuMet: 1.645 ± 0.028
1.604LeuAsn: 1.604 ± 0.029
6.598LeuPro: 6.598 ± 0.067
2.037LeuGln: 2.037 ± 0.028
9.147LeuArg: 9.147 ± 0.074
5.443LeuSer: 5.443 ± 0.044
6.869LeuThr: 6.869 ± 0.057
8.361LeuVal: 8.361 ± 0.076
1.293LeuTrp: 1.293 ± 0.023
1.824LeuTyr: 1.824 ± 0.028
0.0LeuXaa: 0.0 ± 0.0
Met
2.325MetAla: 2.325 ± 0.032
0.147MetCys: 0.147 ± 0.008
0.932MetAsp: 0.932 ± 0.022
0.779MetGlu: 0.779 ± 0.017
0.424MetPhe: 0.424 ± 0.015
1.318MetGly: 1.318 ± 0.025
0.34MetHis: 0.34 ± 0.012
0.621MetIle: 0.621 ± 0.017
0.407MetLys: 0.407 ± 0.014
1.649MetLeu: 1.649 ± 0.027
0.305MetMet: 0.305 ± 0.011
0.393MetAsn: 0.393 ± 0.013
1.141MetPro: 1.141 ± 0.022
0.375MetGln: 0.375 ± 0.013
1.488MetArg: 1.488 ± 0.022
1.321MetSer: 1.321 ± 0.025
1.521MetThr: 1.521 ± 0.026
1.339MetVal: 1.339 ± 0.026
0.219MetTrp: 0.219 ± 0.01
0.329MetTyr: 0.329 ± 0.013
0.0MetXaa: 0.0 ± 0.0
Asn
2.131AsnAla: 2.131 ± 0.031
0.158AsnCys: 0.158 ± 0.009
0.903AsnAsp: 0.903 ± 0.019
0.807AsnGlu: 0.807 ± 0.019
0.435AsnPhe: 0.435 ± 0.013
1.801AsnGly: 1.801 ± 0.032
0.396AsnHis: 0.396 ± 0.012
0.595AsnIle: 0.595 ± 0.016
0.395AsnLys: 0.395 ± 0.015
1.574AsnLeu: 1.574 ± 0.025
0.274AsnMet: 0.274 ± 0.012
0.393AsnAsn: 0.393 ± 0.014
1.266AsnPro: 1.266 ± 0.024
0.474AsnGln: 0.474 ± 0.015
1.27AsnArg: 1.27 ± 0.02
0.83AsnSer: 0.83 ± 0.019
1.072AsnThr: 1.072 ± 0.024
1.272AsnVal: 1.272 ± 0.025
0.255AsnTrp: 0.255 ± 0.011
0.397AsnTyr: 0.397 ± 0.013
0.0AsnXaa: 0.0 ± 0.0
Pro
8.585ProAla: 8.585 ± 0.074
0.379ProCys: 0.379 ± 0.012
4.662ProAsp: 4.662 ± 0.051
4.598ProGlu: 4.598 ± 0.043
1.473ProPhe: 1.473 ± 0.024
7.121ProGly: 7.121 ± 0.068
1.479ProHis: 1.479 ± 0.029
1.314ProIle: 1.314 ± 0.024
1.199ProLys: 1.199 ± 0.026
5.533ProLeu: 5.533 ± 0.054
1.002ProMet: 1.002 ± 0.02
0.836ProAsn: 0.836 ± 0.019
3.863ProPro: 3.863 ± 0.062
1.689ProGln: 1.689 ± 0.03
4.394ProArg: 4.394 ± 0.046
3.343ProSer: 3.343 ± 0.045
3.162ProThr: 3.162 ± 0.04
5.57ProVal: 5.57 ± 0.052
0.909ProTrp: 0.909 ± 0.018
1.375ProTyr: 1.375 ± 0.025
0.0ProXaa: 0.0 ± 0.0
Gln
3.534GlnAla: 3.534 ± 0.041
0.176GlnCys: 0.176 ± 0.008
1.391GlnAsp: 1.391 ± 0.022
1.438GlnGlu: 1.438 ± 0.025
0.641GlnPhe: 0.641 ± 0.017
2.204GlnGly: 2.204 ± 0.033
0.667GlnHis: 0.667 ± 0.021
1.023GlnIle: 1.023 ± 0.022
0.538GlnLys: 0.538 ± 0.017
2.989GlnLeu: 2.989 ± 0.037
0.478GlnMet: 0.478 ± 0.014
0.457GlnAsn: 0.457 ± 0.015
1.705GlnPro: 1.705 ± 0.03
1.184GlnGln: 1.184 ± 0.031
2.586GlnArg: 2.586 ± 0.035
1.21GlnSer: 1.21 ± 0.022
1.3GlnThr: 1.3 ± 0.025
2.241GlnVal: 2.241 ± 0.029
0.464GlnTrp: 0.464 ± 0.014
0.533GlnTyr: 0.533 ± 0.013
0.0GlnXaa: 0.0 ± 0.0
Arg
10.503ArgAla: 10.503 ± 0.087
0.709ArgCys: 0.709 ± 0.017
4.448ArgAsp: 4.448 ± 0.042
5.125ArgGlu: 5.125 ± 0.053
2.364ArgPhe: 2.364 ± 0.032
6.378ArgGly: 6.378 ± 0.049
2.345ArgHis: 2.345 ± 0.031
3.398ArgIle: 3.398 ± 0.035
1.709ArgLys: 1.709 ± 0.032
9.308ArgLeu: 9.308 ± 0.08
1.906ArgMet: 1.906 ± 0.026
1.409ArgAsn: 1.409 ± 0.025
5.443ArgPro: 5.443 ± 0.052
2.464ArgGln: 2.464 ± 0.029
8.329ArgArg: 8.329 ± 0.079
4.246ArgSer: 4.246 ± 0.049
5.718ArgThr: 5.718 ± 0.055
5.94ArgVal: 5.94 ± 0.057
1.471ArgTrp: 1.471 ± 0.024
1.864ArgTyr: 1.864 ± 0.027
0.0ArgXaa: 0.0 ± 0.0
Ser
6.876SerAla: 6.876 ± 0.058
0.44SerCys: 0.44 ± 0.015
2.665SerAsp: 2.665 ± 0.033
2.433SerGlu: 2.433 ± 0.03
1.513SerPhe: 1.513 ± 0.026
6.229SerGly: 6.229 ± 0.06
1.071SerHis: 1.071 ± 0.022
1.342SerIle: 1.342 ± 0.024
0.965SerLys: 0.965 ± 0.023
4.701SerLeu: 4.701 ± 0.048
1.067SerMet: 1.067 ± 0.019
0.801SerAsn: 0.801 ± 0.02
3.22SerPro: 3.22 ± 0.044
1.223SerGln: 1.223 ± 0.024
3.869SerArg: 3.869 ± 0.038
2.86SerSer: 2.86 ± 0.04
2.905SerThr: 2.905 ± 0.036
4.19SerVal: 4.19 ± 0.047
0.892SerTrp: 0.892 ± 0.018
1.167SerTyr: 1.167 ± 0.021
0.0SerXaa: 0.0 ± 0.0
Thr
9.176ThrAla: 9.176 ± 0.078
0.442ThrCys: 0.442 ± 0.015
3.611ThrAsp: 3.611 ± 0.04
3.329ThrGlu: 3.329 ± 0.037
1.56ThrPhe: 1.56 ± 0.027
6.932ThrGly: 6.932 ± 0.06
1.228ThrHis: 1.228 ± 0.026
1.626ThrIle: 1.626 ± 0.031
1.044ThrLys: 1.044 ± 0.025
5.626ThrLeu: 5.626 ± 0.055
0.938ThrMet: 0.938 ± 0.02
0.915ThrAsn: 0.915 ± 0.022
4.126ThrPro: 4.126 ± 0.045
1.228ThrGln: 1.228 ± 0.023
3.974ThrArg: 3.974 ± 0.04
3.167ThrSer: 3.167 ± 0.041
3.832ThrThr: 3.832 ± 0.053
6.058ThrVal: 6.058 ± 0.072
0.844ThrTrp: 0.844 ± 0.021
1.248ThrTyr: 1.248 ± 0.024
0.0ThrXaa: 0.0 ± 0.0
Val
10.566ValAla: 10.566 ± 0.076
0.811ValCys: 0.811 ± 0.018
4.812ValAsp: 4.812 ± 0.048
4.569ValGlu: 4.569 ± 0.047
2.323ValPhe: 2.323 ± 0.032
6.193ValGly: 6.193 ± 0.056
1.99ValHis: 1.99 ± 0.03
2.873ValIle: 2.873 ± 0.042
1.568ValLys: 1.568 ± 0.031
9.3ValLeu: 9.3 ± 0.078
1.425ValMet: 1.425 ± 0.026
1.574ValAsn: 1.574 ± 0.026
5.266ValPro: 5.266 ± 0.051
1.962ValGln: 1.962 ± 0.03
7.361ValArg: 7.361 ± 0.065
4.366ValSer: 4.366 ± 0.045
5.304ValThr: 5.304 ± 0.071
7.708ValVal: 7.708 ± 0.075
1.133ValTrp: 1.133 ± 0.021
1.504ValTyr: 1.504 ± 0.025
0.0ValXaa: 0.0 ± 0.0
Trp
1.659TrpAla: 1.659 ± 0.027
0.171TrpCys: 0.171 ± 0.008
0.805TrpAsp: 0.805 ± 0.017
0.764TrpGlu: 0.764 ± 0.017
0.489TrpPhe: 0.489 ± 0.015
1.077TrpGly: 1.077 ± 0.022
0.386TrpHis: 0.386 ± 0.012
0.538TrpIle: 0.538 ± 0.015
0.353TrpLys: 0.353 ± 0.013
1.775TrpLeu: 1.775 ± 0.025
0.29TrpMet: 0.29 ± 0.01
0.38TrpAsn: 0.38 ± 0.014
0.782TrpPro: 0.782 ± 0.018
0.563TrpGln: 0.563 ± 0.013
1.449TrpArg: 1.449 ± 0.025
0.934TrpSer: 0.934 ± 0.021
1.005TrpThr: 1.005 ± 0.02
0.967TrpVal: 0.967 ± 0.022
0.328TrpTrp: 0.328 ± 0.012
0.349TrpTyr: 0.349 ± 0.012
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.657TyrAla: 2.657 ± 0.029
0.176TyrCys: 0.176 ± 0.008
1.428TyrAsp: 1.428 ± 0.028
1.178TyrGlu: 1.178 ± 0.023
0.608TyrPhe: 0.608 ± 0.019
2.226TyrGly: 2.226 ± 0.029
0.413TyrHis: 0.413 ± 0.01
0.481TyrIle: 0.481 ± 0.013
0.355TyrLys: 0.355 ± 0.013
2.082TyrLeu: 2.082 ± 0.028
0.246TyrMet: 0.246 ± 0.01
0.385TyrAsn: 0.385 ± 0.014
1.078TyrPro: 1.078 ± 0.02
0.594TyrGln: 0.594 ± 0.016
1.881TyrArg: 1.881 ± 0.029
0.897TyrSer: 0.897 ± 0.019
1.18TyrThr: 1.18 ± 0.022
1.559TyrVal: 1.559 ± 0.028
0.353TyrTrp: 0.353 ± 0.013
0.415TyrTyr: 0.415 ± 0.013
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 7825 proteins (2671400 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski