Enterobacteria phage PRD1 (Bacteriophage PRD1)

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Preplasmiviricota; Tectiliviricetes; Kalamavirales; Tectiviridae; Alphatectivirus; Pseudomonas virus PRD1

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 31 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>sp|P27379|PKG6_BPPRD Packaging efficiency factor P6 OS=Enterobacteria phage PRD1 OX=10658 GN=VI PE=1 SV=1
MM1 pKa = 7.78ANFNVPKK8 pKa = 10.64LGVFPVAAVFDD19 pKa = 4.02IDD21 pKa = 4.74NVPEE25 pKa = 4.55DD26 pKa = 3.71SSATGSRR33 pKa = 11.84WLPSIYY39 pKa = 10.04QGGNYY44 pKa = 8.86WGGGPQALHH53 pKa = 5.96AQVSNFDD60 pKa = 3.31SSNRR64 pKa = 11.84LPYY67 pKa = 10.4NPRR70 pKa = 11.84TEE72 pKa = 4.15NNPAGNCAFAFNPFGQYY89 pKa = 9.64ISNISSAQSVHH100 pKa = 5.09RR101 pKa = 11.84RR102 pKa = 11.84IYY104 pKa = 11.01GIDD107 pKa = 3.8LNDD110 pKa = 3.97EE111 pKa = 4.39PLFSPNAASITNGGNPTMSQDD132 pKa = 3.19TGYY135 pKa = 11.27HH136 pKa = 5.98NIGPINTAYY145 pKa = 9.79KK146 pKa = 10.74AEE148 pKa = 4.19IFRR151 pKa = 11.84PVNPLPMSDD160 pKa = 3.49TAPDD164 pKa = 4.21PEE166 pKa = 4.46TLEE169 pKa = 4.57PGQTEE174 pKa = 4.22PLIKK178 pKa = 10.24SDD180 pKa = 3.46GVYY183 pKa = 10.43SNSGIASFIFDD194 pKa = 3.96RR195 pKa = 11.84PVTEE199 pKa = 5.45PNPNWPPLPPPVIPIIYY216 pKa = 6.87PTPALGIGAAAAYY229 pKa = 9.77GFGYY233 pKa = 10.3QVTVYY238 pKa = 8.93RR239 pKa = 11.84WEE241 pKa = 4.45EE242 pKa = 3.81IPVEE246 pKa = 4.9FIADD250 pKa = 3.91PEE252 pKa = 4.38TCPAQPTTDD261 pKa = 3.08KK262 pKa = 11.31VIIRR266 pKa = 11.84TTDD269 pKa = 3.48LNPEE273 pKa = 4.52GSPCAYY279 pKa = 8.77EE280 pKa = 3.86AGIILVRR287 pKa = 11.84QTSNPMNAVAGRR299 pKa = 11.84LVPYY303 pKa = 10.64VEE305 pKa = 6.0DD306 pKa = 3.35IAVDD310 pKa = 3.56IFLTGKK316 pKa = 10.19FFTLNPPLRR325 pKa = 11.84ITNNYY330 pKa = 9.15FADD333 pKa = 4.21DD334 pKa = 3.89EE335 pKa = 4.81VKK337 pKa = 10.83EE338 pKa = 4.11NTVTIGNYY346 pKa = 6.41TTTLSSAYY354 pKa = 8.91YY355 pKa = 10.27AVYY358 pKa = 8.53KK359 pKa = 9.56TDD361 pKa = 3.53GYY363 pKa = 11.61GGATCFIASGGAGISALVQLQDD385 pKa = 3.44NSVLDD390 pKa = 3.49VLYY393 pKa = 11.0YY394 pKa = 10.57SLPLSLGGSKK404 pKa = 10.3AAIDD408 pKa = 3.16EE409 pKa = 4.44WVANNCGLFPMSGGLDD425 pKa = 3.11KK426 pKa = 9.33TTLLEE431 pKa = 3.84IPRR434 pKa = 11.84RR435 pKa = 11.84QLEE438 pKa = 4.58AINPQDD444 pKa = 4.42GPGQYY449 pKa = 11.06DD450 pKa = 4.27LFILDD455 pKa = 4.5DD456 pKa = 4.03SGAYY460 pKa = 10.15ASFSSFIGYY469 pKa = 8.9PEE471 pKa = 3.77AAYY474 pKa = 10.24YY475 pKa = 9.7VAGAATFMDD484 pKa = 4.13VEE486 pKa = 4.52NPDD489 pKa = 3.8EE490 pKa = 4.83IIFILRR496 pKa = 11.84NGAGWYY502 pKa = 10.04ACEE505 pKa = 4.86IGDD508 pKa = 4.32ALKK511 pKa = 10.62IADD514 pKa = 4.81DD515 pKa = 4.18EE516 pKa = 4.74FDD518 pKa = 3.65SVDD521 pKa = 3.22YY522 pKa = 9.99FAYY525 pKa = 10.27RR526 pKa = 11.84GGVMFIGSARR536 pKa = 11.84YY537 pKa = 7.72TEE539 pKa = 5.15GGDD542 pKa = 3.62PLPIKK547 pKa = 10.4YY548 pKa = 9.76RR549 pKa = 11.84AIIPGLPRR557 pKa = 11.84GRR559 pKa = 11.84LPRR562 pKa = 11.84VVLEE566 pKa = 3.96YY567 pKa = 10.53QAVGMSFIPCQTHH580 pKa = 6.33CLGKK584 pKa = 10.66GGIISKK590 pKa = 9.75VV591 pKa = 3.09

Molecular weight: 63.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q3T4N8|VP33_BPPRD Protein P33 OS=Enterobacteria phage PRD1 OX=10658 GN=XXXIII PE=4 SV=1
MM1 pKa = 7.77KK2 pKa = 10.22IFGISVFTIAIIALAYY18 pKa = 10.19YY19 pKa = 10.3LGTKK23 pKa = 10.15RR24 pKa = 11.84IIGG27 pKa = 3.68

Molecular weight: 2.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

30

1

31

5013

27

591

161.7

17.54

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.55 ± 0.893

0.798 ± 0.18

4.668 ± 0.416

5.246 ± 0.663

4.169 ± 0.438

8.777 ± 0.614

0.977 ± 0.233

6.782 ± 0.49

5.306 ± 0.711

7.8 ± 0.438

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.733 ± 0.278

5.067 ± 0.363

5.127 ± 0.541

4.169 ± 0.611

3.75 ± 0.351

5.645 ± 0.467

5.665 ± 0.426

6.723 ± 0.39

1.237 ± 0.144

3.81 ± 0.378
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski