Xenopus tropicalis (Western clawed frog) (Silurana tropicalis)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Metazoa; Eumetazoa; Bilateria; Deuterostomia; Chordata; Craniata; Vertebrata; Gnathostomata; Teleostomi; Euteleostomi; Sarcopterygii; Dipnotetrapodomorpha; Tetrapoda; Amphibia; Batrachia; Anura; Pipoidea

Average proteome isoelectric point is 6.64

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 46313 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A803K5Q7|A0A803K5Q7_XENTR F-box/WD repeat-containing protein 7-like OS=Xenopus tropicalis OX=8364 GN=LOC116410231 PE=4 SV=1
KKK2 pKa = 10.14DD3 pKa = 3.63PTPRR7 pKa = 11.84CLCNQSIGWCRR18 pKa = 11.84LYYY21 pKa = 10.97VNPCCEEE28 pKa = 4.03ALSLVVCSYYY38 pKa = 10.23GQKKK42 pKa = 7.44TQIYYY47 pKa = 8.51GQKKK51 pKa = 9.05PQIYYY56 pKa = 8.75GQKKK60 pKa = 8.98PQIYYY65 pKa = 8.75GQKKK69 pKa = 6.43TQIYYY74 pKa = 8.47GQKKK78 pKa = 6.45TQIYYY83 pKa = 8.47GQKKK87 pKa = 6.45TQIYYY92 pKa = 8.47GQKKK96 pKa = 6.45TQIYYY101 pKa = 8.47GQKKK105 pKa = 6.45TQIYYY110 pKa = 8.47GQKKK114 pKa = 6.45TQIYYY119 pKa = 8.47GQKKK123 pKa = 5.86TQIFTGQKKK132 pKa = 10.03EEE134 pKa = 4.1DDD136 pKa = 4.3DDD138 pKa = 3.63HHH140 pKa = 6.71PEEE143 pKa = 4.2DDD145 pKa = 4.92DDD147 pKa = 3.77HHH149 pKa = 6.71PEEE152 pKa = 4.2DDD154 pKa = 4.92DDD156 pKa = 3.77HHH158 pKa = 6.71PEEE161 pKa = 4.2DDD163 pKa = 4.92DDD165 pKa = 3.77HHH167 pKa = 6.71PEEE170 pKa = 4.2DDD172 pKa = 4.92DDD174 pKa = 3.77HHH176 pKa = 6.71PEEE179 pKa = 4.2DDD181 pKa = 4.92DDD183 pKa = 3.77HHH185 pKa = 6.71PEEE188 pKa = 4.2DDD190 pKa = 4.92DDD192 pKa = 3.77HHH194 pKa = 6.71PEEE197 pKa = 4.2DDD199 pKa = 4.92DDD201 pKa = 3.77HHH203 pKa = 6.71PEEE206 pKa = 4.2DDD208 pKa = 4.92DDD210 pKa = 3.77HHH212 pKa = 6.71PEEE215 pKa = 4.2DDD217 pKa = 4.92DDD219 pKa = 3.77HHH221 pKa = 6.71PEEE224 pKa = 4.2DDD226 pKa = 4.92DDD228 pKa = 3.77HHH230 pKa = 6.71PEEE233 pKa = 4.2DDD235 pKa = 4.92DDD237 pKa = 3.77HHH239 pKa = 6.71PEEE242 pKa = 4.2DDD244 pKa = 4.92DDD246 pKa = 3.77HHH248 pKa = 6.71PEEE251 pKa = 4.2DDD253 pKa = 4.92DDD255 pKa = 3.77HHH257 pKa = 6.71PEEE260 pKa = 4.2DDD262 pKa = 4.92DDD264 pKa = 3.77HHH266 pKa = 6.71PEEE269 pKa = 4.2DDD271 pKa = 4.92DDD273 pKa = 3.77HHH275 pKa = 6.71PEEE278 pKa = 4.2DDD280 pKa = 4.92DDD282 pKa = 3.77HHH284 pKa = 6.71PEEE287 pKa = 4.2DDD289 pKa = 4.92DDD291 pKa = 3.77HHH293 pKa = 6.71PEEE296 pKa = 4.2DDD298 pKa = 4.92DDD300 pKa = 3.77HHH302 pKa = 6.71PEEE305 pKa = 4.2DDD307 pKa = 4.92DDD309 pKa = 3.77HHH311 pKa = 6.71PEEE314 pKa = 4.2DDD316 pKa = 4.92DDD318 pKa = 3.77HHH320 pKa = 6.71PEEE323 pKa = 4.2DDD325 pKa = 4.92DDD327 pKa = 3.77HHH329 pKa = 6.71PEEE332 pKa = 4.2DDD334 pKa = 4.92DDD336 pKa = 3.77HHH338 pKa = 6.71PEEE341 pKa = 4.2DDD343 pKa = 4.92DDD345 pKa = 3.77HHH347 pKa = 6.71PEEE350 pKa = 4.2DDD352 pKa = 4.92DDD354 pKa = 3.77HHH356 pKa = 6.71PEEE359 pKa = 4.2DDD361 pKa = 4.92DDD363 pKa = 3.77HHH365 pKa = 6.71PEEE368 pKa = 4.2DDD370 pKa = 4.92DDD372 pKa = 3.77HHH374 pKa = 6.71PEEE377 pKa = 4.2DDD379 pKa = 5.3DDD381 pKa = 3.72HHH383 pKa = 6.43P

Molecular weight: 45.61 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A803JNG1|A0A803JNG1_XENTR Osteoclast stimulating factor 1 OS=Xenopus tropicalis OX=8364 GN=ostf1 PE=4 SV=1
MM1 pKa = 7.31TGYY4 pKa = 7.86TAAAVSVTPGKK15 pKa = 10.07CCRR18 pKa = 11.84GVHH21 pKa = 5.99KK22 pKa = 10.61LQARR26 pKa = 11.84GMHH29 pKa = 5.72KK30 pKa = 9.25QQARR34 pKa = 11.84GMHH37 pKa = 6.1KK38 pKa = 8.58PQARR42 pKa = 11.84GMHH45 pKa = 6.11KK46 pKa = 8.84PQARR50 pKa = 11.84GMHH53 pKa = 5.6KK54 pKa = 8.96QQARR58 pKa = 11.84GMHH61 pKa = 5.59KK62 pKa = 8.75QQARR66 pKa = 11.84GMHH69 pKa = 6.1KK70 pKa = 8.58PQARR74 pKa = 11.84GMHH77 pKa = 5.6KK78 pKa = 8.96QQARR82 pKa = 11.84GMHH85 pKa = 5.59KK86 pKa = 8.75QQARR90 pKa = 11.84GMHH93 pKa = 5.59KK94 pKa = 8.75QQARR98 pKa = 11.84GMHH101 pKa = 5.59KK102 pKa = 8.75QQARR106 pKa = 11.84GMHH109 pKa = 5.59KK110 pKa = 8.75QQARR114 pKa = 11.84GMHH117 pKa = 6.1KK118 pKa = 8.58PQARR122 pKa = 11.84GMHH125 pKa = 5.6KK126 pKa = 8.96QQARR130 pKa = 11.84GMHH133 pKa = 6.1KK134 pKa = 8.58PQARR138 pKa = 11.84GMHH141 pKa = 5.6KK142 pKa = 8.96QQARR146 pKa = 11.84GMHH149 pKa = 5.59KK150 pKa = 8.75QQARR154 pKa = 11.84GMHH157 pKa = 5.59KK158 pKa = 8.75QQARR162 pKa = 11.84GMHH165 pKa = 5.64KK166 pKa = 8.68QQARR170 pKa = 11.84GTHH173 pKa = 5.09KK174 pKa = 9.34QQARR178 pKa = 11.84GTHH181 pKa = 5.09KK182 pKa = 9.34QQARR186 pKa = 11.84GTHH189 pKa = 5.09KK190 pKa = 9.34QQARR194 pKa = 11.84GTHH197 pKa = 5.09KK198 pKa = 9.34QQARR202 pKa = 11.84GTHH205 pKa = 5.09KK206 pKa = 9.34QQARR210 pKa = 11.84GTHH213 pKa = 5.09KK214 pKa = 9.34QQARR218 pKa = 11.84GTHH221 pKa = 5.09KK222 pKa = 9.34QQARR226 pKa = 11.84GTHH229 pKa = 5.09KK230 pKa = 9.34QQARR234 pKa = 11.84GTHH237 pKa = 6.14KK238 pKa = 10.08LQARR242 pKa = 11.84GTHH245 pKa = 6.34KK246 pKa = 10.43LQARR250 pKa = 11.84GTHH253 pKa = 5.3KK254 pKa = 9.95QQARR258 pKa = 11.84GTHH261 pKa = 6.14KK262 pKa = 10.08LQARR266 pKa = 11.84GTHH269 pKa = 5.3KK270 pKa = 9.95QQARR274 pKa = 11.84GTHH277 pKa = 6.14KK278 pKa = 10.08LQARR282 pKa = 11.84GTHH285 pKa = 5.3KK286 pKa = 9.95QQARR290 pKa = 11.84GTHH293 pKa = 6.14KK294 pKa = 10.08LQARR298 pKa = 11.84GTHH301 pKa = 5.3KK302 pKa = 9.95QQARR306 pKa = 11.84GTHH309 pKa = 6.14KK310 pKa = 10.08LQARR314 pKa = 11.84GTHH317 pKa = 5.3KK318 pKa = 9.95QQARR322 pKa = 11.84GTHH325 pKa = 6.14KK326 pKa = 10.08LQARR330 pKa = 11.84GTHH333 pKa = 5.3KK334 pKa = 9.95QQARR338 pKa = 11.84GTHH341 pKa = 5.06KK342 pKa = 9.4QQARR346 pKa = 11.84GMHH349 pKa = 5.64KK350 pKa = 8.68QQARR354 pKa = 11.84GTHH357 pKa = 6.08KK358 pKa = 10.1LQARR362 pKa = 11.84GMHH365 pKa = 5.72KK366 pKa = 9.25QQARR370 pKa = 11.84GMHH373 pKa = 5.64KK374 pKa = 8.68QQARR378 pKa = 11.84GTHH381 pKa = 6.14KK382 pKa = 10.08LQARR386 pKa = 11.84GTHH389 pKa = 5.3KK390 pKa = 9.95QQARR394 pKa = 11.84GTHH397 pKa = 5.09KK398 pKa = 9.34QQARR402 pKa = 11.84GTHH405 pKa = 5.09KK406 pKa = 9.34QQARR410 pKa = 11.84GTHH413 pKa = 6.08KK414 pKa = 10.1LQARR418 pKa = 11.84GMHH421 pKa = 5.72KK422 pKa = 9.25QQARR426 pKa = 11.84GMHH429 pKa = 5.59KK430 pKa = 8.75QQARR434 pKa = 11.84GMHH437 pKa = 5.59KK438 pKa = 8.75QQARR442 pKa = 11.84GMHH445 pKa = 5.59KK446 pKa = 8.75QQARR450 pKa = 11.84GMHH453 pKa = 5.59KK454 pKa = 8.75QQARR458 pKa = 11.84GMHH461 pKa = 5.66KK462 pKa = 9.9QQANLTAVPIHH473 pKa = 6.65CNNMRR478 pKa = 11.84HH479 pKa = 6.44CII481 pKa = 3.75

Molecular weight: 54.76 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

22514

23799

46313

29680869

18

10837

640.9

71.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.096 ± 0.01

2.308 ± 0.011

5.08 ± 0.008

7.052 ± 0.016

3.685 ± 0.008

5.917 ± 0.017

2.599 ± 0.008

5.103 ± 0.01

6.34 ± 0.016

9.382 ± 0.015

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.274 ± 0.005

4.337 ± 0.007

5.495 ± 0.016

4.722 ± 0.011

5.175 ± 0.012

8.579 ± 0.018

5.748 ± 0.022

6.028 ± 0.01

1.145 ± 0.004

2.935 ± 0.007
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski