Nipah virus
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>sp|Q9IK92|NCAP_NIPAV Nucleoprotein OS=Nipah virus OX=121791 GN=N PE=1 SV=1
MM1 pKa = 8.19 DD2 pKa = 5.21 KK3 pKa = 11.41 LEE5 pKa = 4.44 LVNDD9 pKa = 4.35 GLNIIDD15 pKa = 5.98 FIQKK19 pKa = 7.95 NQKK22 pKa = 9.6 EE23 pKa = 4.2 IQKK26 pKa = 9.07 TYY28 pKa = 10.35 GRR30 pKa = 11.84 SSIQQPSIKK39 pKa = 10.19 DD40 pKa = 3.28 QTKK43 pKa = 8.84 AWEE46 pKa = 4.83 DD47 pKa = 3.53 FLQCTSGEE55 pKa = 4.26 SEE57 pKa = 4.09 QVEE60 pKa = 4.25 GGMSKK65 pKa = 10.96 DD66 pKa = 3.5 DD67 pKa = 3.67 GDD69 pKa = 3.75 VEE71 pKa = 4.45 RR72 pKa = 11.84 RR73 pKa = 11.84 NLEE76 pKa = 4.35 DD77 pKa = 4.48 LSSTSPTDD85 pKa = 3.19 GTIGKK90 pKa = 9.18 RR91 pKa = 11.84 VSNTRR96 pKa = 11.84 DD97 pKa = 2.99 WAEE100 pKa = 3.94 GSDD103 pKa = 5.29 DD104 pKa = 3.66 IQLDD108 pKa = 3.81 PVVTDD113 pKa = 3.48 VVYY116 pKa = 10.5 HH117 pKa = 5.48 DD118 pKa = 5.08 HH119 pKa = 7.06 GGEE122 pKa = 4.01 CTGYY126 pKa = 10.79 GFTSSPEE133 pKa = 4.18 RR134 pKa = 11.84 GWSDD138 pKa = 3.45 YY139 pKa = 11.32 TSGANNGNVCLVSDD153 pKa = 4.63 AKK155 pKa = 9.89 MLSYY159 pKa = 10.77 APEE162 pKa = 3.89 IAVSKK167 pKa = 10.4 EE168 pKa = 3.5 DD169 pKa = 3.68 RR170 pKa = 11.84 EE171 pKa = 4.16 TDD173 pKa = 4.02 LVHH176 pKa = 7.22 LEE178 pKa = 4.42 NKK180 pKa = 10.43 LSTTGLNPTAVPFTLRR196 pKa = 11.84 NLSDD200 pKa = 3.72 PAKK203 pKa = 9.53 DD204 pKa = 3.45 SPVIAEE210 pKa = 4.48 HH211 pKa = 6.52 YY212 pKa = 9.65 YY213 pKa = 10.22 GLGVKK218 pKa = 8.05 EE219 pKa = 4.15 QNVGPQTSRR228 pKa = 11.84 NVNLDD233 pKa = 3.46 SIKK236 pKa = 10.65 LYY238 pKa = 10.66 TSDD241 pKa = 5.95 DD242 pKa = 3.83 EE243 pKa = 5.1 EE244 pKa = 6.27 ADD246 pKa = 3.34 QLEE249 pKa = 4.58 FEE251 pKa = 5.23 DD252 pKa = 4.72 EE253 pKa = 4.41 FAGSSSEE260 pKa = 4.27 VIVGISPEE268 pKa = 4.08 DD269 pKa = 3.79 EE270 pKa = 4.33 EE271 pKa = 4.58 PSSVGGKK278 pKa = 9.12 PNEE281 pKa = 4.58 SIGRR285 pKa = 11.84 TIEE288 pKa = 4.16 GQSIRR293 pKa = 11.84 DD294 pKa = 3.52 NLQAKK299 pKa = 9.31 DD300 pKa = 3.86 NKK302 pKa = 9.73 STDD305 pKa = 3.41 VPGAGPKK312 pKa = 10.25 DD313 pKa = 3.52 SAVKK317 pKa = 10.37 EE318 pKa = 4.11 EE319 pKa = 4.44 PPQKK323 pKa = 9.86 RR324 pKa = 11.84 LPMLAEE330 pKa = 4.08 EE331 pKa = 5.05 FEE333 pKa = 4.85 CSGSEE338 pKa = 3.96 DD339 pKa = 4.05 PIIRR343 pKa = 11.84 EE344 pKa = 3.95 LLKK347 pKa = 10.89 EE348 pKa = 3.86 NSLINCQQGKK358 pKa = 9.45 DD359 pKa = 3.68 AQPPYY364 pKa = 8.9 HH365 pKa = 6.68 WSIEE369 pKa = 4.04 RR370 pKa = 11.84 SISPDD375 pKa = 2.53 KK376 pKa = 11.25 TEE378 pKa = 3.96 IVNGAVQTADD388 pKa = 3.47 RR389 pKa = 11.84 QRR391 pKa = 11.84 PGTPMPKK398 pKa = 9.87 SRR400 pKa = 11.84 GIPIKK405 pKa = 10.52 KK406 pKa = 8.66 GTDD409 pKa = 2.9 AKK411 pKa = 10.69 YY412 pKa = 10.28 PSAGTEE418 pKa = 3.81 NVPGSKK424 pKa = 9.92 SGATRR429 pKa = 11.84 HH430 pKa = 5.28 VRR432 pKa = 11.84 GSPPYY437 pKa = 10.27 QEE439 pKa = 4.47 GKK441 pKa = 10.06 SVNAEE446 pKa = 3.78 NVQLNASTAVKK457 pKa = 8.92 EE458 pKa = 4.36 TDD460 pKa = 3.12 KK461 pKa = 11.81 SEE463 pKa = 4.05 VNPVDD468 pKa = 5.69 DD469 pKa = 5.81 NDD471 pKa = 4.34 SLDD474 pKa = 4.4 DD475 pKa = 4.26 KK476 pKa = 11.55 YY477 pKa = 11.65 IMPSDD482 pKa = 4.05 DD483 pKa = 3.52 FSNTFFPHH491 pKa = 5.87 DD492 pKa = 3.84 TDD494 pKa = 3.38 RR495 pKa = 11.84 LNYY498 pKa = 9.73 HH499 pKa = 7.03 ADD501 pKa = 3.2 HH502 pKa = 6.79 LGDD505 pKa = 3.89 YY506 pKa = 10.71 DD507 pKa = 6.17 LEE509 pKa = 4.55 TLCEE513 pKa = 4.02 EE514 pKa = 4.32 SVLMGVINSIKK525 pKa = 10.39 LINLDD530 pKa = 3.0 MRR532 pKa = 11.84 LNHH535 pKa = 6.87 IEE537 pKa = 4.16 EE538 pKa = 4.25 QVKK541 pKa = 9.97 EE542 pKa = 3.88 IPKK545 pKa = 9.97 IINKK549 pKa = 9.78 LEE551 pKa = 4.5 SIDD554 pKa = 4.02 RR555 pKa = 11.84 VLAKK559 pKa = 10.14 TNTALSTIEE568 pKa = 3.82 GHH570 pKa = 6.27 LVSMMIMIPGKK581 pKa = 10.71 GKK583 pKa = 10.48 GEE585 pKa = 3.69 RR586 pKa = 11.84 KK587 pKa = 9.75 GKK589 pKa = 10.07 NNPEE593 pKa = 4.17 LKK595 pKa = 10.22 PVIGRR600 pKa = 11.84 DD601 pKa = 3.04 ILEE604 pKa = 4.16 QQSLFSFDD612 pKa = 3.07 NVKK615 pKa = 10.48 NFRR618 pKa = 11.84 DD619 pKa = 3.83 GSLTNEE625 pKa = 4.21 PYY627 pKa = 10.58 GAAVQLRR634 pKa = 11.84 EE635 pKa = 4.25 DD636 pKa = 4.91 LILPEE641 pKa = 4.92 LNFEE645 pKa = 4.33 EE646 pKa = 4.79 TNASQFVPMADD657 pKa = 3.3 DD658 pKa = 3.53 SSRR661 pKa = 11.84 DD662 pKa = 3.63 VIKK665 pKa = 9.91 TLIRR669 pKa = 11.84 THH671 pKa = 5.98 IKK673 pKa = 10.44 DD674 pKa = 3.3 RR675 pKa = 11.84 EE676 pKa = 4.2 LRR678 pKa = 11.84 SEE680 pKa = 4.69 LIGYY684 pKa = 7.78 LNKK687 pKa = 10.56 AEE689 pKa = 4.19 NDD691 pKa = 3.48 EE692 pKa = 4.69 EE693 pKa = 4.13 IQEE696 pKa = 4.22 IANTVNDD703 pKa = 4.81 IIDD706 pKa = 3.68 GNII709 pKa = 2.98
Molecular weight: 78.3 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.64
IPC2_protein 4.533
IPC_protein 4.507
Toseland 4.342
ProMoST 4.622
Dawson 4.457
Bjellqvist 4.609
Wikipedia 4.329
Rodwell 4.342
Grimsley 4.253
Solomon 4.457
Lehninger 4.418
Nozaki 4.558
DTASelect 4.736
Thurlkill 4.342
EMBOSS 4.355
Sillero 4.622
Patrickios 4.075
IPC_peptide 4.457
IPC2_peptide 4.609
IPC2.peptide.svr19 4.552
Protein with the highest isoelectric point:
>sp|Q9IK91|PHOSP_NIPAV Phosphoprotein OS=Nipah virus OX=121791 GN=P/V/C PE=1 SV=1
MM1 pKa = 7.51 EE2 pKa = 6.3 PDD4 pKa = 3.13 IKK6 pKa = 10.9 SISSEE11 pKa = 3.97 SMEE14 pKa = 4.14 GVSDD18 pKa = 4.46 FSPSSWEE25 pKa = 3.6 HH26 pKa = 5.58 GGYY29 pKa = 9.7 LDD31 pKa = 4.68 KK32 pKa = 11.44 VEE34 pKa = 4.88 PEE36 pKa = 3.47 IDD38 pKa = 3.8 EE39 pKa = 4.53 NGSMIPKK46 pKa = 9.67 YY47 pKa = 10.46 KK48 pKa = 10.14 IYY50 pKa = 10.47 TPGANEE56 pKa = 3.76 RR57 pKa = 11.84 KK58 pKa = 9.16 YY59 pKa = 11.08 NNYY62 pKa = 9.42 MYY64 pKa = 9.67 LICYY68 pKa = 8.93 GFVEE72 pKa = 4.58 DD73 pKa = 5.22 VEE75 pKa = 4.54 RR76 pKa = 11.84 TPEE79 pKa = 3.53 TGKK82 pKa = 10.18 RR83 pKa = 11.84 KK84 pKa = 9.95 KK85 pKa = 9.99 IRR87 pKa = 11.84 TIAAYY92 pKa = 8.88 PLGVGKK98 pKa = 10.17 SASHH102 pKa = 6.01 PQDD105 pKa = 3.81 LLEE108 pKa = 4.51 EE109 pKa = 4.4 LCSLKK114 pKa = 9.7 VTVRR118 pKa = 11.84 RR119 pKa = 11.84 TAGSTEE125 pKa = 3.97 KK126 pKa = 10.43 IVFGSSGPLNHH137 pKa = 6.8 LVPWKK142 pKa = 10.41 KK143 pKa = 10.57 VLTSGSIFNAVKK155 pKa = 9.82 VCRR158 pKa = 11.84 NVDD161 pKa = 3.48 QIQLDD166 pKa = 3.4 KK167 pKa = 11.11 HH168 pKa = 4.46 QALRR172 pKa = 11.84 IFFLSITKK180 pKa = 10.61 LNDD183 pKa = 2.92 SGIYY187 pKa = 8.39 MIPRR191 pKa = 11.84 TMLEE195 pKa = 3.67 FRR197 pKa = 11.84 RR198 pKa = 11.84 NNAIAFNLLVYY209 pKa = 10.11 LKK211 pKa = 10.14 IDD213 pKa = 3.74 ADD215 pKa = 3.98 LSKK218 pKa = 10.54 MGIQGSLDD226 pKa = 3.2 KK227 pKa = 11.43 DD228 pKa = 3.83 GFKK231 pKa = 10.66 VASFMLHH238 pKa = 6.3 LGNFVRR244 pKa = 11.84 RR245 pKa = 11.84 AGKK248 pKa = 10.17 YY249 pKa = 9.66 YY250 pKa = 10.69 SVDD253 pKa = 3.09 YY254 pKa = 10.53 CRR256 pKa = 11.84 RR257 pKa = 11.84 KK258 pKa = 8.96 IDD260 pKa = 3.4 RR261 pKa = 11.84 MKK263 pKa = 10.9 LQFSLGSIGGLSLHH277 pKa = 6.47 IKK279 pKa = 9.92 INGVISKK286 pKa = 10.48 RR287 pKa = 11.84 LFAQMGFQKK296 pKa = 10.44 NLCFSLMDD304 pKa = 3.9 INPWLNRR311 pKa = 11.84 LTWNNSCEE319 pKa = 4.12 ISRR322 pKa = 11.84 VAAVLQPSIPRR333 pKa = 11.84 EE334 pKa = 3.57 FMIYY338 pKa = 10.7 DD339 pKa = 3.94 DD340 pKa = 4.73 VFIDD344 pKa = 3.35 NTGRR348 pKa = 11.84 ILKK351 pKa = 9.94 GG352 pKa = 3.12
Molecular weight: 39.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 8.736
IPC2_protein 8.639
IPC_protein 8.536
Toseland 9.194
ProMoST 9.033
Dawson 9.502
Bjellqvist 9.311
Wikipedia 9.677
Rodwell 9.736
Grimsley 9.56
Solomon 9.545
Lehninger 9.516
Nozaki 9.428
DTASelect 9.238
Thurlkill 9.37
EMBOSS 9.648
Sillero 9.502
Patrickios 4.762
IPC_peptide 9.545
IPC2_peptide 7.98
IPC2.peptide.svr19 7.764
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
8
0
8
5891
352
2244
736.4
82.6
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.025 ± 0.637
1.731 ± 0.274
6.281 ± 0.535
6.569 ± 0.644
3.191 ± 0.369
6.043 ± 0.699
1.765 ± 0.381
8.046 ± 0.559
6.315 ± 0.268
8.98 ± 0.77
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.326 ± 0.217
5.805 ± 0.316
4.651 ± 0.474
3.768 ± 0.263
5.008 ± 0.324
8.861 ± 0.221
5.483 ± 0.253
5.839 ± 0.277
0.985 ± 0.131
3.327 ± 0.362
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here