Cellvibrio sp. PSBB006
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 4268 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1Y0FSD2|A0A1Y0FSD2_9GAMM Agmatine deiminase OS=Cellvibrio sp. PSBB006 OX=1987723 GN=CBR65_01995 PE=4 SV=1
MM1 pKa = 7.25 FKK3 pKa = 10.54 VLPILLSVFLLISCGGGGGGGSSDD27 pKa = 3.35 GGEE30 pKa = 4.35 TRR32 pKa = 11.84 SSTSSSSSSSEE43 pKa = 3.78 SSSSVTSSSQSSSSDD58 pKa = 3.02 SSSSVAPNEE67 pKa = 4.15 APTASAGEE75 pKa = 4.01 DD76 pKa = 3.28 QIVQGGFTVQLTAAGSADD94 pKa = 3.72 PEE96 pKa = 4.89 GEE98 pKa = 4.1 TLTYY102 pKa = 10.61 EE103 pKa = 4.18 WMQVTEE109 pKa = 4.21 HH110 pKa = 6.69 NIMYY114 pKa = 8.01 VTADD118 pKa = 3.4 GTITEE123 pKa = 4.48 EE124 pKa = 4.81 MDD126 pKa = 3.41 TQDD129 pKa = 3.59 LFVYY133 pKa = 10.46 IPATEE138 pKa = 4.05 AGKK141 pKa = 10.59 EE142 pKa = 4.08 YY143 pKa = 10.14 TFSLTVSDD151 pKa = 4.12 GTHH154 pKa = 5.54 TSEE157 pKa = 5.21 ADD159 pKa = 3.51 TVTLTVADD167 pKa = 4.3 CFEE170 pKa = 5.17 GDD172 pKa = 2.81 GDD174 pKa = 4.75 VFIEE178 pKa = 5.32 CIDD181 pKa = 3.92 PSWFGISSYY190 pKa = 9.88 QEE192 pKa = 3.39 IDD194 pKa = 3.23 GNAGNNFYY202 pKa = 11.38 SNGIDD207 pKa = 3.31 NHH209 pKa = 6.3 VNWSLVDD216 pKa = 4.1 LEE218 pKa = 5.36 DD219 pKa = 4.69 GEE221 pKa = 4.85 HH222 pKa = 6.55 NNVIDD227 pKa = 4.7 VKK229 pKa = 10.2 FLRR232 pKa = 11.84 NDD234 pKa = 2.8 SSGNFTIAAPTPEE247 pKa = 4.47 NADD250 pKa = 3.35 MPAIDD255 pKa = 4.01 MTDD258 pKa = 3.94 FANGYY263 pKa = 9.73 IKK265 pKa = 10.57 FDD267 pKa = 3.41 VRR269 pKa = 11.84 AVGTTDD275 pKa = 3.62 PLTLFLSIQCGYY287 pKa = 9.66 PCGTSTPWTVEE298 pKa = 3.95 TLPDD302 pKa = 3.99 SEE304 pKa = 4.4 WTSVTIWVEE313 pKa = 3.68 NLIDD317 pKa = 4.86 LEE319 pKa = 4.56 LDD321 pKa = 3.3 VSKK324 pKa = 10.91 ISTALLINPQWVNQADD340 pKa = 3.9 VNFQLDD346 pKa = 3.64 NIRR349 pKa = 11.84 WEE351 pKa = 4.24 KK352 pKa = 10.78 ASCDD356 pKa = 3.58 DD357 pKa = 3.71 APNVVFSNCLIQDD370 pKa = 3.75 DD371 pKa = 4.71 RR372 pKa = 11.84 SASGWDD378 pKa = 3.44 EE379 pKa = 4.88 DD380 pKa = 3.81 GGFYY384 pKa = 10.92 GLPDD388 pKa = 4.87 PDD390 pKa = 4.26 NHH392 pKa = 6.64 ISWLEE397 pKa = 3.79 IFSGDD402 pKa = 3.5 EE403 pKa = 3.67 GHH405 pKa = 6.68 NDD407 pKa = 3.32 VIEE410 pKa = 4.03 VSFNNDD416 pKa = 2.9 TQSSSFSIIPVPLASMDD433 pKa = 3.91 LSAYY437 pKa = 10.07 AAGEE441 pKa = 4.05 MVFDD445 pKa = 4.36 YY446 pKa = 11.47 KK447 pKa = 10.55 MVSNPHH453 pKa = 6.8 ADD455 pKa = 3.38 TNLFATVQCGDD466 pKa = 3.51 WPCAGGEE473 pKa = 3.99 FLLDD477 pKa = 3.37 KK478 pKa = 11.33 SEE480 pKa = 4.29 FGVWKK485 pKa = 10.19 EE486 pKa = 3.82 MRR488 pKa = 11.84 ISVADD493 pKa = 4.17 MIDD496 pKa = 3.25 NDD498 pKa = 4.3 LDD500 pKa = 3.49 IMQVSTWFTLIPTYY514 pKa = 10.23 GQPQSGIRR522 pKa = 11.84 VEE524 pKa = 4.05 LDD526 pKa = 3.12 NIRR529 pKa = 11.84 WEE531 pKa = 4.21 EE532 pKa = 3.78
Molecular weight: 57.66 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.715
IPC2_protein 3.617
IPC_protein 3.656
Toseland 3.427
ProMoST 3.808
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.579
Rodwell 3.478
Grimsley 3.338
Solomon 3.643
Lehninger 3.592
Nozaki 3.757
DTASelect 3.999
Thurlkill 3.478
EMBOSS 3.579
Sillero 3.77
Patrickios 0.909
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.733
Protein with the highest isoelectric point:
>tr|A0A1Y0FYY1|A0A1Y0FYY1_9GAMM Uncharacterized protein OS=Cellvibrio sp. PSBB006 OX=1987723 GN=CBR65_15130 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLKK11 pKa = 9.95 RR12 pKa = 11.84 VRR14 pKa = 11.84 NHH16 pKa = 5.46 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.35 NGRR28 pKa = 11.84 LVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.14 GRR39 pKa = 11.84 KK40 pKa = 8.34 QLTII44 pKa = 3.64
Molecular weight: 5.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
4268
0
4268
1542520
38
5127
361.4
40.03
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.426 ± 0.041
0.935 ± 0.013
5.799 ± 0.035
5.878 ± 0.034
3.981 ± 0.024
7.174 ± 0.054
2.304 ± 0.024
5.898 ± 0.027
4.098 ± 0.039
10.345 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.308 ± 0.018
4.242 ± 0.05
4.376 ± 0.025
4.445 ± 0.033
5.397 ± 0.038
6.396 ± 0.042
5.538 ± 0.04
6.895 ± 0.028
1.465 ± 0.018
3.099 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here