Mycobacterium phage Sibs6

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 6.18

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345MBT3|A0A345MBT3_9CAUD Minor tail protein OS=Mycobacterium phage Sibs6 OX=2283259 GN=29 PE=4 SV=1
MM1 pKa = 7.61GFTLRR6 pKa = 11.84LFGIPVLSLEE16 pKa = 4.13ITGDD20 pKa = 3.64GSAEE24 pKa = 3.94EE25 pKa = 4.62YY26 pKa = 10.64INLTGGSFEE35 pKa = 4.65LAPEE39 pKa = 3.98EE40 pKa = 4.15PEE42 pKa = 3.9YY43 pKa = 11.13DD44 pKa = 3.29EE45 pKa = 5.61EE46 pKa = 4.95YY47 pKa = 10.82YY48 pKa = 10.93EE49 pKa = 4.22EE50 pKa = 4.6DD51 pKa = 3.1RR52 pKa = 11.84SGFGFGVSS60 pKa = 3.28

Molecular weight: 6.63 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345MBR7|A0A345MBR7_9CAUD Terminase OS=Mycobacterium phage Sibs6 OX=2283259 GN=13 PE=4 SV=1
MM1 pKa = 7.41AVHH4 pKa = 6.12YY5 pKa = 10.0PEE7 pKa = 5.3SLLPAPKK14 pKa = 10.21VCAHH18 pKa = 7.06CEE20 pKa = 3.77QPMPEE25 pKa = 4.45GKK27 pKa = 9.84RR28 pKa = 11.84PHH30 pKa = 6.72AVYY33 pKa = 10.55CDD35 pKa = 3.78RR36 pKa = 11.84RR37 pKa = 11.84CKK39 pKa = 9.08TAAAEE44 pKa = 4.25TRR46 pKa = 11.84RR47 pKa = 11.84PPRR50 pKa = 11.84DD51 pKa = 2.83NHH53 pKa = 5.74ARR55 pKa = 11.84YY56 pKa = 9.51LKK58 pKa = 10.02EE59 pKa = 3.8RR60 pKa = 11.84EE61 pKa = 3.59RR62 pKa = 11.84RR63 pKa = 11.84LAYY66 pKa = 10.09SRR68 pKa = 11.84EE69 pKa = 4.04YY70 pKa = 10.44QKK72 pKa = 11.09RR73 pKa = 11.84NPDD76 pKa = 2.99VPKK79 pKa = 9.28RR80 pKa = 11.84AKK82 pKa = 9.75RR83 pKa = 11.84KK84 pKa = 9.28RR85 pKa = 11.84RR86 pKa = 11.84ALIAGRR92 pKa = 11.84EE93 pKa = 4.24TFTITQRR100 pKa = 11.84DD101 pKa = 3.51WLRR104 pKa = 11.84LVRR107 pKa = 11.84RR108 pKa = 11.84HH109 pKa = 5.26NHH111 pKa = 4.71RR112 pKa = 11.84CFYY115 pKa = 10.86CEE117 pKa = 3.77APGPMSMDD125 pKa = 2.84HH126 pKa = 5.95VVPISRR132 pKa = 11.84GGRR135 pKa = 11.84HH136 pKa = 6.09SIGNIVPACISCNSSKK152 pKa = 10.49RR153 pKa = 11.84DD154 pKa = 3.35RR155 pKa = 11.84TVVEE159 pKa = 3.66WRR161 pKa = 11.84LSKK164 pKa = 10.37RR165 pKa = 11.84GHH167 pKa = 6.03IARR170 pKa = 11.84VAA172 pKa = 3.13

Molecular weight: 20.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

15813

23

826

166.5

18.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.941 ± 0.419

0.689 ± 0.129

6.59 ± 0.231

6.596 ± 0.268

3.2 ± 0.2

8.771 ± 0.578

2.062 ± 0.183

4.907 ± 0.252

4.186 ± 0.288

8.487 ± 0.242

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.074 ± 0.133

3.219 ± 0.137

5.533 ± 0.242

3.168 ± 0.167

6.539 ± 0.412

5.774 ± 0.264

6.28 ± 0.321

7.076 ± 0.217

2.017 ± 0.126

2.89 ± 0.182
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski